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The map label for this gene is ilvI [H]

Identifier: 13471456

GI number: 13471456

Start: 1195159

End: 1196940

Strand: Reverse

Name: ilvI [H]

Synonym: mll1433

Alternate gene names: 13471456

Gene position: 1196940-1195159 (Counterclockwise)

Preceding gene: 13471459

Following gene: 13471455

Centisome position: 17.01

GC content: 62.96

Gene sequence:

>1782_bases
ATGAGCAACGGACAAAGTGAACGGCGCGAGATGACAGGCGCCGAAATGGTGGTGCAGGCGCTGAAGGACAATGGCGTCAA
GCATGTCTTCGGCTACCCGGGCGGCGCGGTTCTCCCGATCTATGACGAGATCTTCCAGCAGGACGACGTCGAGCACATTC
TCGTGCGGCACGAGCAAGGTGCCGGGCATGCGGCCGAGGGCTATGCGCGCTCGACCGGCAAGGCCGGCGTCATGCTGGTG
ACGTCAGGCCCCGGCGCGACCAATGCGGTGACGCCGCTGCAGGATGCGCTGATGGATTCGATCCCGCTGGTCTGCCTGAC
GGGCCAGGTGCCGACCTCCTTGATCGGCTCCGACGCGTTCCAGGAATGCGACACGGTCGGTATTACGCGCCCCTGCACCA
AGCACAACTGGCTGGTGAAGGACGTCAACGAGCTCGCCGCGACCATCCATGAGGCTTTCCACGTCGCGACCACGGGCCGT
CCGGGGCCGGTTGTGGTCGACATCCCCAAGGACGTGCAGTTCGCCAAGGGTTTCTACGTCCCGCCGCAGATCGCGCCGCG
CACCAGCTACCAGCCCAAGGTTCAGGGCGACCTGGAGAAGATCAAGGCCGCGGTCGAGCTGATGGCCAGCGCCAAGAAGC
CGATCATCTATTCGGGCGGCGGCGTGATCAATTCCGGTCCGGAAGCGAGCCATCTGCTGCGCGAACTGGTCGACCTTACC
GGTTTCCCGATCACCTCGACGCTGATGGGGCTCGGCGCCTATCCGGCGTCGGGCAAGAACTGGGTCGGCATGCTCGGCAT
GCACGGCACCTACGAGGCCAACATGGCCATGCATGATTGCGACGTGATGATCTGCATCGGCGCGCGCTTCGACGACCGCA
TCACCGGCCGGCTCAATGCGTTCTCGCCGAACTCGAAGAAGATCCACATCGACATCGACCCGTCCTCGATCAACAAGAAC
GTGCACACCGAGGTGCCGATCATCGGCGATGTCGGCCGGGTGCTGGAGGATATGGTGCGGCTGTGGCGGGCAACCGCCAA
GGCCGACAAGAAGGCGCTCTATCCCTGGTGGGAGCAGATCGCAAAATGGCGTGGGCGCGATTCACTCGCCTACAAGATGA
ACCACGACGTGATCATGCCGCAATACGCCATCCAGCGGCTCTACGAACTGACCAAGGACAAGGACACCTACATCACCACC
GAAGTCGGCCAACACCAGATGTGGGCGGCGCAGCACTATCATTTCGAAAAGCCGAACCGCTGGATGACCTCAGGCGGTCT
GGGCACGATGGGCTATGGCCTGCCGGCGGCACTCGGCGTGCAGATCGCGCATCCCGATGCGCTGGTCATCGACATTGCCG
GCGACGCCTCGGTGCAGATGACGATGCAGGAGATGAGTGCCGCGGTGCAGTACAAGGCGCCGATCAAGATCTTCATCCTC
AACAACCAGTATATGGGCATGGTGCGGCAATGGCAGCAGCTCTTGCACGGCAACCGGCTGTCGCATTCCTACACCGAGGC
GATGCCTGACTTTGTCAAGCTGGCGGAGGCCTATGGCGGCCATGGCATACGCTGCGAGAAGCCGGACGAGCTGGACGACG
CCATCAAGGAGATGATCTCGGTCCGGAAACCCGTGCTGTTCGACTGCCGCGTGGCGAACCTCGCCAACTGCTTCCCGATG
ATCCCGTCCGGCAAGGCGCATAACGAGATGCTGTTGCCGGACGAGGCCACCGACGAGGCCGTGGCCAACGCCATCGACGC
CAAGGGCAGGGAACTGGTTTAA

Upstream 100 bases:

>100_bases
AGGCTCCCTCGGGGGCCTTTTTTATTGCCCGAAACACGACTAAACGACCAACAACACCCAAATGGCAACGCCCAGATGGC
GACAGTACGGGACCAGACCG

Downstream 100 bases:

>100_bases
TCGACGGGCCAAGGCAGGGCTTGTCGAAGAAGCCGTGCGAAAACAAAGAATTAGATCAAGAATTTGAGATCCATGTCATG
AACGCACAGCACCTTCAACC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MSNGQSERREMTGAEMVVQALKDNGVKHVFGYPGGAVLPIYDEIFQQDDVEHILVRHEQGAGHAAEGYARSTGKAGVMLV
TSGPGATNAVTPLQDALMDSIPLVCLTGQVPTSLIGSDAFQECDTVGITRPCTKHNWLVKDVNELAATIHEAFHVATTGR
PGPVVVDIPKDVQFAKGFYVPPQIAPRTSYQPKVQGDLEKIKAAVELMASAKKPIIYSGGGVINSGPEASHLLRELVDLT
GFPITSTLMGLGAYPASGKNWVGMLGMHGTYEANMAMHDCDVMICIGARFDDRITGRLNAFSPNSKKIHIDIDPSSINKN
VHTEVPIIGDVGRVLEDMVRLWRATAKADKKALYPWWEQIAKWRGRDSLAYKMNHDVIMPQYAIQRLYELTKDKDTYITT
EVGQHQMWAAQHYHFEKPNRWMTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASVQMTMQEMSAAVQYKAPIKIFIL
NNQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGGHGIRCEKPDELDDAIKEMISVRKPVLFDCRVANLANCFPM
IPSGKAHNEMLLPDEATDEAVANAIDAKGRELV

Sequences:

>Translated_593_residues
MSNGQSERREMTGAEMVVQALKDNGVKHVFGYPGGAVLPIYDEIFQQDDVEHILVRHEQGAGHAAEGYARSTGKAGVMLV
TSGPGATNAVTPLQDALMDSIPLVCLTGQVPTSLIGSDAFQECDTVGITRPCTKHNWLVKDVNELAATIHEAFHVATTGR
PGPVVVDIPKDVQFAKGFYVPPQIAPRTSYQPKVQGDLEKIKAAVELMASAKKPIIYSGGGVINSGPEASHLLRELVDLT
GFPITSTLMGLGAYPASGKNWVGMLGMHGTYEANMAMHDCDVMICIGARFDDRITGRLNAFSPNSKKIHIDIDPSSINKN
VHTEVPIIGDVGRVLEDMVRLWRATAKADKKALYPWWEQIAKWRGRDSLAYKMNHDVIMPQYAIQRLYELTKDKDTYITT
EVGQHQMWAAQHYHFEKPNRWMTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASVQMTMQEMSAAVQYKAPIKIFIL
NNQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGGHGIRCEKPDELDDAIKEMISVRKPVLFDCRVANLANCFPM
IPSGKAHNEMLLPDEATDEAVANAIDAKGRELV
>Mature_592_residues
SNGQSERREMTGAEMVVQALKDNGVKHVFGYPGGAVLPIYDEIFQQDDVEHILVRHEQGAGHAAEGYARSTGKAGVMLVT
SGPGATNAVTPLQDALMDSIPLVCLTGQVPTSLIGSDAFQECDTVGITRPCTKHNWLVKDVNELAATIHEAFHVATTGRP
GPVVVDIPKDVQFAKGFYVPPQIAPRTSYQPKVQGDLEKIKAAVELMASAKKPIIYSGGGVINSGPEASHLLRELVDLTG
FPITSTLMGLGAYPASGKNWVGMLGMHGTYEANMAMHDCDVMICIGARFDDRITGRLNAFSPNSKKIHIDIDPSSINKNV
HTEVPIIGDVGRVLEDMVRLWRATAKADKKALYPWWEQIAKWRGRDSLAYKMNHDVIMPQYAIQRLYELTKDKDTYITTE
VGQHQMWAAQHYHFEKPNRWMTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASVQMTMQEMSAAVQYKAPIKIFILN
NQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGGHGIRCEKPDELDDAIKEMISVRKPVLFDCRVANLANCFPMI
PSGKAHNEMLLPDEATDEAVANAIDAKGRELV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=573, Percent_Identity=25.130890052356, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI21361361, Length=566, Percent_Identity=24.3816254416961, Blast_Score=152, Evalue=8e-37,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=50.7042253521127, Blast_Score=583, Evalue=1e-167,
Organism=Escherichia coli, GI1790104, Length=572, Percent_Identity=41.4335664335664, Blast_Score=443, Evalue=1e-125,
Organism=Escherichia coli, GI1786717, Length=567, Percent_Identity=33.5097001763668, Blast_Score=311, Evalue=8e-86,
Organism=Escherichia coli, GI1787096, Length=552, Percent_Identity=29.5289855072464, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1788716, Length=565, Percent_Identity=25.1327433628319, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=553, Percent_Identity=27.124773960217, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17531301, Length=553, Percent_Identity=27.124773960217, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=528, Percent_Identity=26.7045454545455, Blast_Score=138, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=45.360824742268, Blast_Score=513, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6320816, Length=492, Percent_Identity=24.7967479674797, Blast_Score=116, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6321524, Length=548, Percent_Identity=21.8978102189781, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323073, Length=542, Percent_Identity=22.3247232472325, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922626, Length=553, Percent_Identity=26.4014466546112, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 65060; Mature: 64929

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNGQSERREMTGAEMVVQALKDNGVKHVFGYPGGAVLPIYDEIFQQDDVEHILVRHEQG
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEHHHHHHCCCCCHHHHHEEECCC
AGHAAEGYARSTGKAGVMLVTSGPGATNAVTPLQDALMDSIPLVCLTGQVPTSLIGSDAF
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCCHHHHCCHHH
QECDTVGITRPCTKHNWLVKDVNELAATIHEAFHVATTGRPGPVVVDIPKDVQFAKGFYV
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCEEEECCCCCHHHCCCCC
PPQIAPRTSYQPKVQGDLEKIKAAVELMASAKKPIIYSGGGVINSGPEASHLLRELVDLT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHC
GFPITSTLMGLGAYPASGKNWVGMLGMHGTYEANMAMHDCDVMICIGARFDDRITGRLNA
CCCHHHHHHHCCCCCCCCCCCEEEECCCCCEECCEEEECCCEEEEECCCCCCCCCCEEEC
FSPNSKKIHIDIDPSSINKNVHTEVPIIGDVGRVLEDMVRLWRATAKADKKALYPWWEQI
CCCCCCEEEEEECHHHCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
AKWRGRDSLAYKMNHDVIMPQYAIQRLYELTKDKDTYITTEVGQHQMWAAQHYHFEKPNR
HHHCCCCCEEEECCCCEECHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCC
WMTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASVQMTMQEMSAAVQYKAPIKIFIL
EEECCCCCCCCCCCHHHHCEEEECCCEEEEEECCCCCHHHHHHHHHHHHEECCCEEEEEE
NNQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGGHGIRCEKPDELDDAIKEMIS
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
VRKPVLFDCRVANLANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRELV
HCCCEEEEEEHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SNGQSERREMTGAEMVVQALKDNGVKHVFGYPGGAVLPIYDEIFQQDDVEHILVRHEQG
CCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEHHHHHHCCCCCHHHHHEEECCC
AGHAAEGYARSTGKAGVMLVTSGPGATNAVTPLQDALMDSIPLVCLTGQVPTSLIGSDAF
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCCHHHHCCHHH
QECDTVGITRPCTKHNWLVKDVNELAATIHEAFHVATTGRPGPVVVDIPKDVQFAKGFYV
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCEEEECCCCCHHHCCCCC
PPQIAPRTSYQPKVQGDLEKIKAAVELMASAKKPIIYSGGGVINSGPEASHLLRELVDLT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHC
GFPITSTLMGLGAYPASGKNWVGMLGMHGTYEANMAMHDCDVMICIGARFDDRITGRLNA
CCCHHHHHHHCCCCCCCCCCCEEEECCCCCEECCEEEECCCEEEEECCCCCCCCCCEEEC
FSPNSKKIHIDIDPSSINKNVHTEVPIIGDVGRVLEDMVRLWRATAKADKKALYPWWEQI
CCCCCCEEEEEECHHHCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
AKWRGRDSLAYKMNHDVIMPQYAIQRLYELTKDKDTYITTEVGQHQMWAAQHYHFEKPNR
HHHCCCCCEEEECCCCEECHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCC
WMTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASVQMTMQEMSAAVQYKAPIKIFIL
EEECCCCCCCCCCCHHHHCEEEECCCEEEEEECCCCCHHHHHHHHHHHHEECCCEEEEEE
NNQYMGMVRQWQQLLHGNRLSHSYTEAMPDFVKLAEAYGGHGIRCEKPDELDDAIKEMIS
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
VRKPVLFDCRVANLANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRELV
HCCCEEEEEEHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]