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The map label for this gene is nuoB [H]

Identifier: 13471406

GI number: 13471406

Start: 1146622

End: 1147224

Strand: Reverse

Name: nuoB [H]

Synonym: mll1371

Alternate gene names: 13471406

Gene position: 1147224-1146622 (Counterclockwise)

Preceding gene: 13471407

Following gene: 13471405

Centisome position: 16.3

GC content: 63.18

Gene sequence:

>603_bases
ATGGAAAAAAGGAGCGCTGGAATGGGATTGAACGACAGTTCAGGCACCCTCGTCGCGCCGAAGCCGAAGGGCATCATCGA
TCCCAACACCGGCAGGCCGGTGGGGGAGGATGATCCCTTCTTCCTCGAAATCAACAATGAGCTCGCCGACAAGGGGTTCC
TGGTCACCTCGACCGAGGCGCTGATCACCTGGGCGCGCAGCGGCTCGCTGATGTTCATGACCTTCGGTCTGGCATGCTGC
GCCGTCGAGATGATCCACACCTCGATGCCGCGCTATGACTCGGAGCGCTTCGGTGTCGCGCCGCGCGCATCGCCGCGCCA
GTCCGACATCATGATCGTCGCCGGCACGCTGACCAACAAGATGGCGCCGGCGCTGCGCAAGGTCTACGACCAGATGCCGG
AGCCGCGCTACGTCATCTCGATGGGCTCCTGCGCCAATGGCGGCGGCTACTACCACTATTCCTACTCGGTGGTGCGCGGC
TGCGACCGCATCGTGCCGGTCGACATCTATGTGCCCGGCTGCCCGCCGAGCGCCGAAGCTTTGCTCTACGGCATTCTTCT
CCTGCAGAAGAAAATCCGCCGCACCGGCACGATCGAACGGTAA

Upstream 100 bases:

>100_bases
TCCTGTTTCCCTGGGCCGTGTCCTTCTCGAAGATCGGCATGCTCGGCTTCTGGTCGATGATGGTGTTTTTGGCAGTGCTG
ACCATCGGCTTTGCCTATGA

Downstream 100 bases:

>100_bases
ATCCATGGCCGCATCCCTTAGCGAACTGTCGACCTATCTCGGCGAAAGGCTGATTGGTCGCGTCAACGACGCTGATATCG
CCTATGGCGAGCTCACCATG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MEKRSAGMGLNDSSGTLVAPKPKGIIDPNTGRPVGEDDPFFLEINNELADKGFLVTSTEALITWARSGSLMFMTFGLACC
AVEMIHTSMPRYDSERFGVAPRASPRQSDIMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRG
CDRIVPVDIYVPGCPPSAEALLYGILLLQKKIRRTGTIER

Sequences:

>Translated_200_residues
MEKRSAGMGLNDSSGTLVAPKPKGIIDPNTGRPVGEDDPFFLEINNELADKGFLVTSTEALITWARSGSLMFMTFGLACC
AVEMIHTSMPRYDSERFGVAPRASPRQSDIMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRG
CDRIVPVDIYVPGCPPSAEALLYGILLLQKKIRRTGTIER
>Mature_200_residues
MEKRSAGMGLNDSSGTLVAPKPKGIIDPNTGRPVGEDDPFFLEINNELADKGFLVTSTEALITWARSGSLMFMTFGLACC
AVEMIHTSMPRYDSERFGVAPRASPRQSDIMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRG
CDRIVPVDIYVPGCPPSAEALLYGILLLQKKIRRTGTIER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=77.5510204081633, Blast_Score=248, Evalue=2e-66,
Organism=Escherichia coli, GI1788624, Length=175, Percent_Identity=45.1428571428571, Blast_Score=157, Evalue=4e-40,
Organism=Escherichia coli, GI1789074, Length=126, Percent_Identity=44.4444444444444, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=41.4634146341463, Blast_Score=102, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17509685, Length=173, Percent_Identity=60.6936416184971, Blast_Score=223, Evalue=3e-59,
Organism=Drosophila melanogaster, GI18859983, Length=139, Percent_Identity=76.9784172661871, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24642371, Length=139, Percent_Identity=76.9784172661871, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24651058, Length=154, Percent_Identity=70.7792207792208, Blast_Score=240, Evalue=4e-64,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 21864; Mature: 21864

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKRSAGMGLNDSSGTLVAPKPKGIIDPNTGRPVGEDDPFFLEINNELADKGFLVTSTEA
CCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEHHH
LITWARSGSLMFMTFGLACCAVEMIHTSMPRYDSERFGVAPRASPRQSDIMIVAGTLTNK
EEEEECCCCEEHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCEEEEEECHHHH
MAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPSAEA
HHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHH
LLYGILLLQKKIRRTGTIER
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEKRSAGMGLNDSSGTLVAPKPKGIIDPNTGRPVGEDDPFFLEINNELADKGFLVTSTEA
CCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEHHH
LITWARSGSLMFMTFGLACCAVEMIHTSMPRYDSERFGVAPRASPRQSDIMIVAGTLTNK
EEEEECCCCEEHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCEEEEEECHHHH
MAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPSAEA
HHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHH
LLYGILLLQKKIRRTGTIER
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]