Click here to switch to the map view.

The map label for this gene is ytcB [H]

Identifier: 13471300

GI number: 13471300

Start: 1038398

End: 1039321

Strand: Reverse

Name: ytcB [H]

Synonym: mll1234

Alternate gene names: 13471300

Gene position: 1039321-1038398 (Counterclockwise)

Preceding gene: 13471307

Following gene: 13471297

Centisome position: 14.77

GC content: 62.55

Gene sequence:

>924_bases
ATGCGAATCTTGCTCACGGGATCGTCGGGTTGGCTGGGCAGCGCGCTGGCGCCGCGCCTGCGCGCGCTTGGCCATGAGGT
GATCGGCCTCGACCCTGTCCCTTCGGTTGAAACGCAGATGATCGGCTCGATCGCCGATCGCGATCTGGTCATGCAGGTGA
TCGGGGATAATCGAATCGAAGCCATCATTCACAGCGGCGCGCTGCACAAGCCCAATATAGAGAGCCGCGCCAACAGCGAT
TTCGTCGCCACCAATGTGCAAGGCACGCTGAACCTGCTCGACGCAGCGGTGGCGAGCGGCGTGCAGCGCTTCGTCTTCAC
CTCGACCACCTCCCTGATGATCTCGCAGGCGATACGCGACGGCTTCAAGGGCGGTGCGCGCAAGGCCGCCTGGCTGACCG
AGGCGATGTCGCCCGAGCCGCGCAACATCTATGGCGTCACCAAACTTTCGGCCGAACATCTTTGCCGCCTCTACCATCTG
CGGCATGGCCTGCCGGTGGTCGTGCTGCGCACGGCCCGTTTCTTCCCCGAAGCCGATGACATGGCGCATACGATCGAACA
ATCAGATGCCAACACCAAGGCAAACGAATTGCTGTTCCGCCGGCTGACGGTGGAAGACGCGGCGGAAGCCCATGTCGCTG
CCCTGGAGAAGGCGCCGCAGCTTGGCTTCGATACTTTTATCATCTGTGCGCCGACGCCGTTTCGGCCCGACGATTGCGAT
GCGTTGATCAGGGATGCGCCAGCCGTCGTCGCACGCCATTTCCCGGACTTTCCGACGCTTTATGCGCGAAAGGGCTGGAC
GATGTTTTCCTCCATCGACCGTGTCTATGACGCGTCGCGAGCAAGAGACCGGCTGGGTTTTGTCTGCAAGACGAGCTTTG
CGGACGTGCTGGCGGCGCTAAGGGCGGAGGACGAGGCCGCCTAG

Upstream 100 bases:

>100_bases
CGGAGCGGCACCATTACCGGCCGATAGCTGATTTTACCGGGTCGCTCGCGCCTGTCATCCGTGCTTAACAGCGTCGCCAT
CAGAAATCATGGTGTGACGC

Downstream 100 bases:

>100_bases
CGCATTTGGATCAGGCGACCTTTGCCGCTTGCGCCATTTCGATCGTCAGGTCGCCGTAGCCGGTCGCGCGCCGCTCATAG
GCAAGGCCGAGCAGGGCGGC

Product: UDP-glucose 4-epimerase

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRILLTGSSGWLGSALAPRLRALGHEVIGLDPVPSVETQMIGSIADRDLVMQVIGDNRIEAIIHSGALHKPNIESRANSD
FVATNVQGTLNLLDAAVASGVQRFVFTSTTSLMISQAIRDGFKGGARKAAWLTEAMSPEPRNIYGVTKLSAEHLCRLYHL
RHGLPVVVLRTARFFPEADDMAHTIEQSDANTKANELLFRRLTVEDAAEAHVAALEKAPQLGFDTFIICAPTPFRPDDCD
ALIRDAPAVVARHFPDFPTLYARKGWTMFSSIDRVYDASRARDRLGFVCKTSFADVLAALRAEDEAA

Sequences:

>Translated_307_residues
MRILLTGSSGWLGSALAPRLRALGHEVIGLDPVPSVETQMIGSIADRDLVMQVIGDNRIEAIIHSGALHKPNIESRANSD
FVATNVQGTLNLLDAAVASGVQRFVFTSTTSLMISQAIRDGFKGGARKAAWLTEAMSPEPRNIYGVTKLSAEHLCRLYHL
RHGLPVVVLRTARFFPEADDMAHTIEQSDANTKANELLFRRLTVEDAAEAHVAALEKAPQLGFDTFIICAPTPFRPDDCD
ALIRDAPAVVARHFPDFPTLYARKGWTMFSSIDRVYDASRARDRLGFVCKTSFADVLAALRAEDEAA
>Mature_307_residues
MRILLTGSSGWLGSALAPRLRALGHEVIGLDPVPSVETQMIGSIADRDLVMQVIGDNRIEAIIHSGALHKPNIESRANSD
FVATNVQGTLNLLDAAVASGVQRFVFTSTTSLMISQAIRDGFKGGARKAAWLTEAMSPEPRNIYGVTKLSAEHLCRLYHL
RHGLPVVVLRTARFFPEADDMAHTIEQSDANTKANELLFRRLTVEDAAEAHVAALEKAPQLGFDTFIICAPTPFRPDDCD
ALIRDAPAVVARHFPDFPTLYARKGWTMFSSIDRVYDASRARDRLGFVCKTSFADVLAALRAEDEAA

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Escherichia coli, GI1786974, Length=190, Percent_Identity=27.3684210526316, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 33555; Mature: 33555

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILLTGSSGWLGSALAPRLRALGHEVIGLDPVPSVETQMIGSIADRDLVMQVIGDNRIE
CEEEEECCCCCCHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
AIIHSGALHKPNIESRANSDFVATNVQGTLNLLDAAVASGVQRFVFTSTTSLMISQAIRD
HHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFKGGARKAAWLTEAMSPEPRNIYGVTKLSAEHLCRLYHLRHGLPVVVLRTARFFPEADD
HHCCCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCHHH
MAHTIEQSDANTKANELLFRRLTVEDAAEAHVAALEKAPQLGFDTFIICAPTPFRPDDCD
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHH
ALIRDAPAVVARHFPDFPTLYARKGWTMFSSIDRVYDASRARDRLGFVCKTSFADVLAAL
HHHHCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAEDEAA
HHCCCCC
>Mature Secondary Structure
MRILLTGSSGWLGSALAPRLRALGHEVIGLDPVPSVETQMIGSIADRDLVMQVIGDNRIE
CEEEEECCCCCCHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
AIIHSGALHKPNIESRANSDFVATNVQGTLNLLDAAVASGVQRFVFTSTTSLMISQAIRD
HHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFKGGARKAAWLTEAMSPEPRNIYGVTKLSAEHLCRLYHLRHGLPVVVLRTARFFPEADD
HHCCCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCHHH
MAHTIEQSDANTKANELLFRRLTVEDAAEAHVAALEKAPQLGFDTFIICAPTPFRPDDCD
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHH
ALIRDAPAVVARHFPDFPTLYARKGWTMFSSIDRVYDASRARDRLGFVCKTSFADVLAAL
HHHHCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAEDEAA
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]