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The map label for this gene is ptrB [C]

Identifier: 13471282

GI number: 13471282

Start: 1014452

End: 1016515

Strand: Reverse

Name: ptrB [C]

Synonym: mll1209

Alternate gene names: 13471282

Gene position: 1016515-1014452 (Counterclockwise)

Preceding gene: 13471283

Following gene: 13471281

Centisome position: 14.45

GC content: 64.49

Gene sequence:

>2064_bases
ATGACCAAGCCCATCACCAAGCCAGAAGCCGGCGCCGCGGATCCCTTCCTCTGGCTGGAGGACAGGACCGCCAAACAGTC
GCTCGACTGGGTACATGGCCAGAACGAGATCGCGGTCGGCGAATTGCAGGGCGATCCGTCCTATCAGGCGTCGTTCCAGA
CGGCGCTCGACCTGATGACGGCCGAGGACAATATCGCCGTCGGCGCGGCGATCGCCGGCCATGTCTATAATTTCTGGCAG
GACAAGACCAATGCGCTCGGCCAGTGGCGCCGCACGACGGTCGCTTCCTACAAGACCGAGAAGCCGGTCTGGGAAACCAT
CATCGACTTCGACCAGCTTGCGGCGAAGGAGGGGATAAAATGGGTGTTCAGCGGCGCCAGCCGGCTCTATCCGGACTTCA
GCCGCTGCCTGCTCTCGATGTCGCCCGATGGTGGCGACGCCAGCGAGATGCGCGAATTCGACATCGAAACCAGATCGTTC
ATCGAGGGCGGGTTTTGTGCGCCGGCTTCCAAGTCGAATTTCGGCTGGCTGGACAAGGATACGGTCATCGTCTCGGCCGC
CTTCGAGGATGCCGACAAGACCGAGTCCGGCTATCCGCGCGTGATCAAGCTGTGGAAACGCGGCACGGCGCTGGAAGAGG
CGACGCCGATCTTCGAAGCGCAGAAAGACGATCTCGCCGCTGGCGCGGGCGTCGAGTTCGACGGCGACAAGCGACACGTG
TTTCTGGCGCGGGCAATCAATTTCTTCGCATCGCACAGTTTCCTGCATCTCCCATCCGGTGAGAACAGGCGCATTCCGCT
GCCTGACGATGCCACCGATACATCGCTTTTCAAGGACCAGCTGGTGTTCGGGGTGCGCACGCCGTGGACGGCGCCGGACG
GCACGGTGTGCCAGCCGGACGGGCTCTATTCGCTCGATTTCGCCCACTGGATCGAAACCGGCAGTTTCGGCGCCATCGAA
ATCCTGCTGGCGCCGGCGCATCGCGTGTCCATCGCCGGGCTCGCCAGAACGCAGGGCCGGCTGTTCATCAGCCTGATGGA
CAATGTGCGCGGCAAGGTCCTCGTCTGCGAGCGCAGGGATGGCGCATGGTCGCTGAAGCCGCTCGCGTTGCCCGAGAACG
GCACTGTCGGCATCAGCCATGCCGAGCATTTCGGCTCCAGCGTGTCCTTCTCCTTCACGGATTTCCTGACGCCGAGCTCG
ATCATCTGGTCGGATGATGATGGCGAGACGCTGGAGACGGTGAAGTCGCAGCCGGCGCGCTTCGACGCGTCGCCGCTGAT
CTCGGAGCAGTTCGAGGCGCGCTCGAAGGACGGCACGATGATCCCTTATTTCGTCGTCCGGCGACGCGACCAGAAGGGGC
CGGTGCCGACGCTGCTCTACGGCTATGGCGGCTTCGAAGTGCCGCTCTTGCCCGGCTATGCCGGGGTGCGCGGCAGGCTG
TGGCTGGAGAAGGGCAATGCCTATGTGCAGGCCTGCATCCGTGGCGGCGGCGAGTTCGGCCCGGCCTGGCATCAGGCGGC
GCTGAAGGGCAATCGCCAGAACGGTTTTGACGATTTCGCCGCTGTGGCTGAGGACGTCGTCAGACGTGGCATCGCGACAG
CAAGCTCGCTCGGCATCCAGGGCGGCTCGAATGGCGGCCTGCTGACCGGCGTTTCCCTGACGCAGCGCCCGGAACTCTTT
GGCGCCGTCATCATCGAGGTGCCGCTGCTCGACATGTTGCGCTACACCGAATTGCCGCCCGGCGCCTCATGGATGGCAGA
ATATGGCGATCCGTCGAAGCCGGAAGACGCCAGGTGGCTGTCCGCCTATTCGCCCTACCAGCATGTCAGGGCGGGCGCCG
CCTATCCGCCGGTGCTTCTGACCACCTCGACCGCCGATGACCGCGTCCATCCCGGCCATGCGCGCAAGATGGCGGCTCGC
CTGCAGGAGGCCGGCCACGACAGGACATTGTTCTTCGAGGAAACCGAAGGCGGGCATGGCGGACGGGGCGATCGCCGGCC
GCAGGCGGCGCAGACGGCGATGAAATATGTCTTCCTGCAGCGGGCACTGAGCGCCAAGGCTTGA

Upstream 100 bases:

>100_bases
GCGAGAAGGCCCGAATCCTCTGGCCAGCTGCCGCAACTCTTGAATATCGGACGCGGCATGTCGATGTTGCATGCCCACAA
GCATGCCTATTGGAGGTTTC

Downstream 100 bases:

>100_bases
AGTGCGTCGCGACGCACTTCATGCCTTTGTTTTATGCATGTCGTTCTCCTAAAACCGGGGCCACTTTTAGGCGACATGCA
TAGAAGTATTCCGTCATGGG

Product: endopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MTKPITKPEAGAADPFLWLEDRTAKQSLDWVHGQNEIAVGELQGDPSYQASFQTALDLMTAEDNIAVGAAIAGHVYNFWQ
DKTNALGQWRRTTVASYKTEKPVWETIIDFDQLAAKEGIKWVFSGASRLYPDFSRCLLSMSPDGGDASEMREFDIETRSF
IEGGFCAPASKSNFGWLDKDTVIVSAAFEDADKTESGYPRVIKLWKRGTALEEATPIFEAQKDDLAAGAGVEFDGDKRHV
FLARAINFFASHSFLHLPSGENRRIPLPDDATDTSLFKDQLVFGVRTPWTAPDGTVCQPDGLYSLDFAHWIETGSFGAIE
ILLAPAHRVSIAGLARTQGRLFISLMDNVRGKVLVCERRDGAWSLKPLALPENGTVGISHAEHFGSSVSFSFTDFLTPSS
IIWSDDDGETLETVKSQPARFDASPLISEQFEARSKDGTMIPYFVVRRRDQKGPVPTLLYGYGGFEVPLLPGYAGVRGRL
WLEKGNAYVQACIRGGGEFGPAWHQAALKGNRQNGFDDFAAVAEDVVRRGIATASSLGIQGGSNGGLLTGVSLTQRPELF
GAVIIEVPLLDMLRYTELPPGASWMAEYGDPSKPEDARWLSAYSPYQHVRAGAAYPPVLLTTSTADDRVHPGHARKMAAR
LQEAGHDRTLFFEETEGGHGGRGDRRPQAAQTAMKYVFLQRALSAKA

Sequences:

>Translated_687_residues
MTKPITKPEAGAADPFLWLEDRTAKQSLDWVHGQNEIAVGELQGDPSYQASFQTALDLMTAEDNIAVGAAIAGHVYNFWQ
DKTNALGQWRRTTVASYKTEKPVWETIIDFDQLAAKEGIKWVFSGASRLYPDFSRCLLSMSPDGGDASEMREFDIETRSF
IEGGFCAPASKSNFGWLDKDTVIVSAAFEDADKTESGYPRVIKLWKRGTALEEATPIFEAQKDDLAAGAGVEFDGDKRHV
FLARAINFFASHSFLHLPSGENRRIPLPDDATDTSLFKDQLVFGVRTPWTAPDGTVCQPDGLYSLDFAHWIETGSFGAIE
ILLAPAHRVSIAGLARTQGRLFISLMDNVRGKVLVCERRDGAWSLKPLALPENGTVGISHAEHFGSSVSFSFTDFLTPSS
IIWSDDDGETLETVKSQPARFDASPLISEQFEARSKDGTMIPYFVVRRRDQKGPVPTLLYGYGGFEVPLLPGYAGVRGRL
WLEKGNAYVQACIRGGGEFGPAWHQAALKGNRQNGFDDFAAVAEDVVRRGIATASSLGIQGGSNGGLLTGVSLTQRPELF
GAVIIEVPLLDMLRYTELPPGASWMAEYGDPSKPEDARWLSAYSPYQHVRAGAAYPPVLLTTSTADDRVHPGHARKMAAR
LQEAGHDRTLFFEETEGGHGGRGDRRPQAAQTAMKYVFLQRALSAKA
>Mature_686_residues
TKPITKPEAGAADPFLWLEDRTAKQSLDWVHGQNEIAVGELQGDPSYQASFQTALDLMTAEDNIAVGAAIAGHVYNFWQD
KTNALGQWRRTTVASYKTEKPVWETIIDFDQLAAKEGIKWVFSGASRLYPDFSRCLLSMSPDGGDASEMREFDIETRSFI
EGGFCAPASKSNFGWLDKDTVIVSAAFEDADKTESGYPRVIKLWKRGTALEEATPIFEAQKDDLAAGAGVEFDGDKRHVF
LARAINFFASHSFLHLPSGENRRIPLPDDATDTSLFKDQLVFGVRTPWTAPDGTVCQPDGLYSLDFAHWIETGSFGAIEI
LLAPAHRVSIAGLARTQGRLFISLMDNVRGKVLVCERRDGAWSLKPLALPENGTVGISHAEHFGSSVSFSFTDFLTPSSI
IWSDDDGETLETVKSQPARFDASPLISEQFEARSKDGTMIPYFVVRRRDQKGPVPTLLYGYGGFEVPLLPGYAGVRGRLW
LEKGNAYVQACIRGGGEFGPAWHQAALKGNRQNGFDDFAAVAEDVVRRGIATASSLGIQGGSNGGLLTGVSLTQRPELFG
AVIIEVPLLDMLRYTELPPGASWMAEYGDPSKPEDARWLSAYSPYQHVRAGAAYPPVLLTTSTADDRVHPGHARKMAARL
QEAGHDRTLFFEETEGGHGGRGDRRPQAAQTAMKYVFLQRALSAKA

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=364, Percent_Identity=35.1648351648352, Blast_Score=201, Evalue=3e-51,
Organism=Homo sapiens, GI284172438, Length=223, Percent_Identity=30.4932735426009, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI284172431, Length=223, Percent_Identity=30.4932735426009, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI284172420, Length=223, Percent_Identity=30.4932735426009, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI284172413, Length=223, Percent_Identity=30.4932735426009, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI70778815, Length=223, Percent_Identity=30.4932735426009, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI108860686, Length=223, Percent_Identity=30.4932735426009, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI108860692, Length=217, Percent_Identity=29.9539170506912, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1788150, Length=347, Percent_Identity=29.6829971181556, Blast_Score=150, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24583414, Length=363, Percent_Identity=34.435261707989, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI221510989, Length=320, Percent_Identity=33.4375, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 75184; Mature: 75053

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPITKPEAGAADPFLWLEDRTAKQSLDWVHGQNEIAVGELQGDPSYQASFQTALDLMT
CCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHC
AEDNIAVGAAIAGHVYNFWQDKTNALGQWRRTTVASYKTEKPVWETIIDFDQLAAKEGIK
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCE
WVFSGASRLYPDFSRCLLSMSPDGGDASEMREFDIETRSFIEGGFCAPASKSNFGWLDKD
EEECCCHHCCCCHHHHHEECCCCCCCHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCC
TVIVSAAFEDADKTESGYPRVIKLWKRGTALEEATPIFEAQKDDLAAGAGVEFDGDKRHV
CEEEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHCCCHHCCCCCCCCCCCCEECCCCCEE
FLARAINFFASHSFLHLPSGENRRIPLPDDATDTSLFKDQLVFGVRTPWTAPDGTVCQPD
HHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCCCCCCEECCC
GLYSLDFAHWIETGSFGAIEILLAPAHRVSIAGLARTQGRLFISLMDNVRGKVLVCERRD
CCEEECHHHHHCCCCCCEEEEEEECCHHEEEHHHECCCCCEEEEEHHCCCCEEEEEECCC
GAWSLKPLALPENGTVGISHAEHFGSSVSFSFTDFLTPSSIIWSDDDGETLETVKSQPAR
CCCCCCEEECCCCCCEECCHHHHCCCCCEEEHHHCCCCCCEEEECCCCHHHHHHHCCCCC
FDASPLISEQFEARSKDGTMIPYFVVRRRDQKGPVPTLLYGYGGFEVPLLPGYAGVRGRL
CCCCCHHHHHHHHCCCCCCCCHHHEEEECCCCCCCCEEEEECCCEECEECCCCCCCCEEE
WLEKGNAYVQACIRGGGEFGPAWHQAALKGNRQNGFDDFAAVAEDVVRRGIATASSLGIQ
EEECCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GGSNGGLLTGVSLTQRPELFGAVIIEVPLLDMLRYTELPPGASWMAEYGDPSKPEDARWL
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHH
SAYSPYQHVRAGAAYPPVLLTTSTADDRVHPGHARKMAARLQEAGHDRTLFFEETEGGHG
HHCCCHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCC
GRGDRRPQAAQTAMKYVFLQRALSAKA
CCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKPITKPEAGAADPFLWLEDRTAKQSLDWVHGQNEIAVGELQGDPSYQASFQTALDLMT
CCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHC
AEDNIAVGAAIAGHVYNFWQDKTNALGQWRRTTVASYKTEKPVWETIIDFDQLAAKEGIK
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCE
WVFSGASRLYPDFSRCLLSMSPDGGDASEMREFDIETRSFIEGGFCAPASKSNFGWLDKD
EEECCCHHCCCCHHHHHEECCCCCCCHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCC
TVIVSAAFEDADKTESGYPRVIKLWKRGTALEEATPIFEAQKDDLAAGAGVEFDGDKRHV
CEEEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHCCCHHCCCCCCCCCCCCEECCCCCEE
FLARAINFFASHSFLHLPSGENRRIPLPDDATDTSLFKDQLVFGVRTPWTAPDGTVCQPD
HHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCCCCCCEECCC
GLYSLDFAHWIETGSFGAIEILLAPAHRVSIAGLARTQGRLFISLMDNVRGKVLVCERRD
CCEEECHHHHHCCCCCCEEEEEEECCHHEEEHHHECCCCCEEEEEHHCCCCEEEEEECCC
GAWSLKPLALPENGTVGISHAEHFGSSVSFSFTDFLTPSSIIWSDDDGETLETVKSQPAR
CCCCCCEEECCCCCCEECCHHHHCCCCCEEEHHHCCCCCCEEEECCCCHHHHHHHCCCCC
FDASPLISEQFEARSKDGTMIPYFVVRRRDQKGPVPTLLYGYGGFEVPLLPGYAGVRGRL
CCCCCHHHHHHHHCCCCCCCCHHHEEEECCCCCCCCEEEEECCCEECEECCCCCCCCEEE
WLEKGNAYVQACIRGGGEFGPAWHQAALKGNRQNGFDDFAAVAEDVVRRGIATASSLGIQ
EEECCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GGSNGGLLTGVSLTQRPELFGAVIIEVPLLDMLRYTELPPGASWMAEYGDPSKPEDARWL
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHH
SAYSPYQHVRAGAAYPPVLLTTSTADDRVHPGHARKMAARLQEAGHDRTLFFEETEGGHG
HHCCCHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCC
GRGDRRPQAAQTAMKYVFLQRALSAKA
CCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.23 {tosyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]