Click here to switch to the map view.

The map label for this gene is argD [C]

Identifier: 13471281

GI number: 13471281

Start: 1012745

End: 1014124

Strand: Reverse

Name: argD [C]

Synonym: mll1207

Alternate gene names: 13471281

Gene position: 1014124-1012745 (Counterclockwise)

Preceding gene: 13471282

Following gene: 13471280

Centisome position: 14.41

GC content: 63.12

Gene sequence:

>1380_bases
ATGACCTATCAGAATTATTCGCTGAAGCAGCTTCAGCAGATCGACGCCGCGCATCATCTCCATCCCTTCACCGACCACAA
GGAATTGCGCGAGGCGGGTTCGCGCATCATCACCCACGCCAATGGCCCGTTCATCTACGATTCCGAAGGGACGGAATTGC
TCGACGGCATGGCCGGCCTGTGGTGCGTCAACATCGGCTATGGCCGCGACGAACTCGCCGAGGCCGCTTACGCGCAGATG
AAGGAACTGCCTTACTACAATTCCTTCTTCAAATGCTCGACGCCGACCCCGGTGCTGTTGTCCAAGAAACTGGCCGAGAT
CGCGCCGAAGAACGTCAATCAGGTCTTCTACGGCTCCTCCGGCTCGGAAGCCAACGACACCGCCTTGCGTCTTGTCCGCC
ACTACTGGGTGATCGAGGGCAAACCCGAGAAGAACCGCGTCATCTCGCGCAAGATGGCCTATCACGGCTCGACCATCGCC
GGCACGTCGCTCGGCGGCATGGATGCCATGCACAAGCAGCTCAACGGAGCGGTGCCCAACATCGTGCATGTGATGATGCC
CTATGCCTATGAGCTGGCGCTGCCGGGTGAGAGCGACCATGATTTCGGCCTGCGCGCGGCCAAATCGGTCGAGGATGCCA
TCCTCGAGGCCGGCGTCGACAAGGTCGCGGCCTTCATCGGCGAGCCGGTAATGGGCGCGGGCGGCGTCAAGATCCCGCCG
GCGAGCTATTGGCCTGAGGTGCAGCGCATCTGCCGCAAATACGATGTGCTGCTGATGCTGGACGAGGTCATCACCGGCTA
TGGCCGTACCGGCGAATGGTTCGCAGCCCAGACCTTCGGCATCGAACCGGACACCATCACCACGGCCAAGGCGCTGACCT
CGGGCTATCAGCCGCTGTCGGCGCTGCTGGTCGGCGACCGCATCACCTCGACGCTGGTCGAGAAGGGCGGCGAGTTCAAC
CACGGCTACACCTATTCCGGCCACCCCGTGGCCTGCGCCGTCGCCTTGAAGAACCTGGAGATCATCGAGCAGGAAGGCCT
GGTCGACCGCGTCAGGAACGACACCGGGCCCTATTTCGCCAAGGCGCTGCAGGAGTGTATCGCCGGGCATGACCTGGTCG
GCGAGGTGCGTTCGATCGGGCTGATGGGCGCGATCGAGATCGTCAAGGACAAGGCGACCAAGGAACGGTTCCTGCCGTCG
GGAAGTGCGGCGGTGACGGTGCGCGACCATGCCATCGCCAACGGCATGATGCTGCGCGCCACCGGTGACACGATGATCCT
GTCGCCGCCGCTGATCTGGACCCGCGACACGATCGACATGGCCTGCGAGCGCATAGGCAAGGCGCTGGATCTGGCGCAGG
CCGATCTGCGCAAGCGGTAA

Upstream 100 bases:

>100_bases
TTTTGCGGCTGTGCGCCCGCATCGGTTATTCGCCGCCCATCAGCCTCTGATGCATTCGCCCCGGCGCCTGCCGGACTGAA
TCCAGAGGAAAGAATCAGAC

Downstream 100 bases:

>100_bases
GGCAAACCTCGACCGGGCGTTTCTTCAAGGGAACGCCCGGCCTCGAGAAACATACTGCCCGATGGGGAACAAACTTCATC
CTCCGCGCGTAGTGTGTGGG

Product: putative aminotransferase

Products: N-Acetyl-L-glutamate 5-semialdehyde; L-Glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MTYQNYSLKQLQQIDAAHHLHPFTDHKELREAGSRIITHANGPFIYDSEGTELLDGMAGLWCVNIGYGRDELAEAAYAQM
KELPYYNSFFKCSTPTPVLLSKKLAEIAPKNVNQVFYGSSGSEANDTALRLVRHYWVIEGKPEKNRVISRKMAYHGSTIA
GTSLGGMDAMHKQLNGAVPNIVHVMMPYAYELALPGESDHDFGLRAAKSVEDAILEAGVDKVAAFIGEPVMGAGGVKIPP
ASYWPEVQRICRKYDVLLMLDEVITGYGRTGEWFAAQTFGIEPDTITTAKALTSGYQPLSALLVGDRITSTLVEKGGEFN
HGYTYSGHPVACAVALKNLEIIEQEGLVDRVRNDTGPYFAKALQECIAGHDLVGEVRSIGLMGAIEIVKDKATKERFLPS
GSAAVTVRDHAIANGMMLRATGDTMILSPPLIWTRDTIDMACERIGKALDLAQADLRKR

Sequences:

>Translated_459_residues
MTYQNYSLKQLQQIDAAHHLHPFTDHKELREAGSRIITHANGPFIYDSEGTELLDGMAGLWCVNIGYGRDELAEAAYAQM
KELPYYNSFFKCSTPTPVLLSKKLAEIAPKNVNQVFYGSSGSEANDTALRLVRHYWVIEGKPEKNRVISRKMAYHGSTIA
GTSLGGMDAMHKQLNGAVPNIVHVMMPYAYELALPGESDHDFGLRAAKSVEDAILEAGVDKVAAFIGEPVMGAGGVKIPP
ASYWPEVQRICRKYDVLLMLDEVITGYGRTGEWFAAQTFGIEPDTITTAKALTSGYQPLSALLVGDRITSTLVEKGGEFN
HGYTYSGHPVACAVALKNLEIIEQEGLVDRVRNDTGPYFAKALQECIAGHDLVGEVRSIGLMGAIEIVKDKATKERFLPS
GSAAVTVRDHAIANGMMLRATGDTMILSPPLIWTRDTIDMACERIGKALDLAQADLRKR
>Mature_458_residues
TYQNYSLKQLQQIDAAHHLHPFTDHKELREAGSRIITHANGPFIYDSEGTELLDGMAGLWCVNIGYGRDELAEAAYAQMK
ELPYYNSFFKCSTPTPVLLSKKLAEIAPKNVNQVFYGSSGSEANDTALRLVRHYWVIEGKPEKNRVISRKMAYHGSTIAG
TSLGGMDAMHKQLNGAVPNIVHVMMPYAYELALPGESDHDFGLRAAKSVEDAILEAGVDKVAAFIGEPVMGAGGVKIPPA
SYWPEVQRICRKYDVLLMLDEVITGYGRTGEWFAAQTFGIEPDTITTAKALTSGYQPLSALLVGDRITSTLVEKGGEFNH
GYTYSGHPVACAVALKNLEIIEQEGLVDRVRNDTGPYFAKALQECIAGHDLVGEVRSIGLMGAIEIVKDKATKERFLPSG
SAAVTVRDHAIANGMMLRATGDTMILSPPLIWTRDTIDMACERIGKALDLAQADLRKR

Specific function: Involved In Both The Arginine And Lysine Biosynthetic Pathways. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI24119277, Length=432, Percent_Identity=27.5462962962963, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI37574042, Length=432, Percent_Identity=28.7037037037037, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI226442705, Length=429, Percent_Identity=28.9044289044289, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI4557809, Length=436, Percent_Identity=27.7522935779816, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI13994255, Length=426, Percent_Identity=26.056338028169, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI226442709, Length=400, Percent_Identity=29, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI284507298, Length=334, Percent_Identity=29.940119760479, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI188536080, Length=323, Percent_Identity=23.5294117647059, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI38679950, Length=323, Percent_Identity=23.5294117647059, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI38679946, Length=323, Percent_Identity=23.5294117647059, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789759, Length=416, Percent_Identity=30.0480769230769, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI145693181, Length=413, Percent_Identity=28.0871670702179, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1788044, Length=418, Percent_Identity=29.9043062200957, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1786991, Length=449, Percent_Identity=28.2850779510022, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1789016, Length=417, Percent_Identity=28.0575539568345, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1787560, Length=410, Percent_Identity=29.7560975609756, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1786349, Length=316, Percent_Identity=25.3164556962025, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71992977, Length=427, Percent_Identity=27.6346604215457, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25144271, Length=431, Percent_Identity=29.0023201856148, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25144274, Length=252, Percent_Identity=34.9206349206349, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564660, Length=446, Percent_Identity=27.5784753363229, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17541228, Length=444, Percent_Identity=23.1981981981982, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323470, Length=446, Percent_Identity=29.1479820627803, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6324386, Length=474, Percent_Identity=28.6919831223629, Blast_Score=146, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6324432, Length=407, Percent_Identity=26.2899262899263, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321456, Length=292, Percent_Identity=27.7397260273973, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21356575, Length=414, Percent_Identity=29.4685990338164, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI161085790, Length=430, Percent_Identity=27.4418604651163, Blast_Score=141, Evalue=8e-34,
Organism=Drosophila melanogaster, GI28574759, Length=428, Percent_Identity=27.3364485981308, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21357415, Length=438, Percent_Identity=27.8538812785388, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI281366494, Length=479, Percent_Identity=23.3820459290188, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667139, Length=479, Percent_Identity=23.3820459290188, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667143, Length=479, Percent_Identity=23.3820459290188, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.11 [C]

Molecular weight: Translated: 50195; Mature: 50064

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYQNYSLKQLQQIDAAHHLHPFTDHKELREAGSRIITHANGPFIYDSEGTELLDGMAGL
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEECCCCEEECCCCHHHHHCCCCE
WCVNIGYGRDELAEAAYAQMKELPYYNSFFKCSTPTPVLLSKKLAEIAPKNVNQVFYGSS
EEEECCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEECCC
GSEANDTALRLVRHYWVIEGKPEKNRVISRKMAYHGSTIAGTSLGGMDAMHKQLNGAVPN
CCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCHH
IVHVMMPYAYELALPGESDHDFGLRAAKSVEDAILEAGVDKVAAFIGEPVMGAGGVKIPP
HHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
ASYWPEVQRICRKYDVLLMLDEVITGYGRTGEWFAAQTFGIEPDTITTAKALTSGYQPLS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCHHH
ALLVGDRITSTLVEKGGEFNHGYTYSGHPVACAVALKNLEIIEQEGLVDRVRNDTGPYFA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCHHHHHHCCCCHHHH
KALQECIAGHDLVGEVRSIGLMGAIEIVKDKATKERFLPSGSAAVTVRDHAIANGMMLRA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHCCEEEEE
TGDTMILSPPLIWTRDTIDMACERIGKALDLAQADLRKR
CCCEEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TYQNYSLKQLQQIDAAHHLHPFTDHKELREAGSRIITHANGPFIYDSEGTELLDGMAGL
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEECCCCEEECCCCHHHHHCCCCE
WCVNIGYGRDELAEAAYAQMKELPYYNSFFKCSTPTPVLLSKKLAEIAPKNVNQVFYGSS
EEEECCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEECCC
GSEANDTALRLVRHYWVIEGKPEKNRVISRKMAYHGSTIAGTSLGGMDAMHKQLNGAVPN
CCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCHH
IVHVMMPYAYELALPGESDHDFGLRAAKSVEDAILEAGVDKVAAFIGEPVMGAGGVKIPP
HHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
ASYWPEVQRICRKYDVLLMLDEVITGYGRTGEWFAAQTFGIEPDTITTAKALTSGYQPLS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCHHH
ALLVGDRITSTLVEKGGEFNHGYTYSGHPVACAVALKNLEIIEQEGLVDRVRNDTGPYFA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCHHHHHHCCCCHHHH
KALQECIAGHDLVGEVRSIGLMGAIEIVKDKATKERFLPSGSAAVTVRDHAIANGMMLRA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHCCEEEEE
TGDTMILSPPLIWTRDTIDMACERIGKALDLAQADLRKR
CCCEEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5-phosphate [C]

Metal ions: Ni2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.1 {N2-acetyl-L-ornithine}} 1.54 {N2-acetyl-L-ornithine}} 1.3 {N2-acetyl-L-ornithine}} 1.1 {N2-acetyl-L-ornithine}} 0.48 {N2-acetyl-L-ornithine}} 0.34 {N2-acetyl-L-ornithine}} 2.5 {2-oxoglutarate}} [C]

Substrates: N2-Acetyl-L-ornithine; 2-Oxoglutarate [C]

Specific reaction: N2-Acetyl-L-ornithine + 2-Oxoglutarate <==> N-Acetyl-L-glutamate 5-semialdehyde + L-Glutamate [C]

General reaction: Amino group transfer [C]

Inhibitor: Cu2+; Ornithine; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]