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The map label for this gene is ilvD2

Identifier: 13471195

GI number: 13471195

Start: 920263

End: 922023

Strand: Reverse

Name: ilvD2

Synonym: mll1102

Alternate gene names: 13471195

Gene position: 922023-920263 (Counterclockwise)

Preceding gene: 13471196

Following gene: 13471194

Centisome position: 13.1

GC content: 64.51

Gene sequence:

>1761_bases
ATGCGGCAGGCCGCACCGCCCGCGGCAAGGATGAGAATGGACGCCAAGGTCATTTCAAAGGCTAAGCTCCCTAGCCGCCA
TGTGACTGTCGGTCCGGCGCGTGCGCCGCACCGGTCCTATCTCTATGCCATGGGGCTTTCCGCCGCCGAGATCGCGCAGC
CGCTGGTCGGCGTCGCCTCCTGCTGGAACGAAGCCGCGCCCTGCAACATCTCGCTGATGCGCCAGGCGCAGGTGGTCAAG
AAGGGTGTCGCCGCCGCCAACGGCACACCGCGCGAATTCTGCACCATCACCGTCACCGACGGCATCGCCATGGGCCACCA
GGGCATGAAGTCGTCGCTGGTGTCGCGCGAGGTCATCGCCGATTCGGTCGAGCTCACGATGCGTGGCCATTGCTACGATG
CGCTGGTCGGCCTCGCCGGCTGCGACAAGTCGCTGCCCGGCATGATGATGGCCATGGTGCGGCTCAACGTGCCGTCGATC
TTCATCTATGGCGGTTCGATCCTGCCCGGCAGCTATCGCGGCCGTCAGATTACCGTGCAGGACGTGTTCGAGGCGGTCGG
CCAGCACTCCGTCGGCACGATCGGCGACGCCGAGCTGCTCGAGATCGAGCAGGCGGCCTGTCCGTCGGCCGGCTCCTGCG
GCGCCCAGTTCACCGCCAACACCATGGCAACCGTCGCCGAGGCGATCGGTCTGGCGCTGCCCTATTCCTGCGGCGCGCCG
GCGCCTTACGAAATGCGCGACCGCTTCAATTTCGCCTCCGGCGAAAAGATCATGGAGCTGATCGCCAAAAACATCCGGCC
GCGCGACATCATCACGCTGAAGGCGCTGGAGAACGCGGCGACCGTGGTTTCCGCCACCGGCGGTTCGACCAATGCGGCGC
TGCATCTGCCGGCGATCGCGCATGAGGCGGGCATCAAATTCGATCTCTTCGATGTCGCAAGGATTTTCGAGAAGACGCCC
TACATCGCCGACCTCAAGCCTGGCGGCAAATATGTCGCAAAGGACATGTTCGAGGCCGGCGGCATTCCGCTGTTGATGAA
GACGCTGCTCGACCACGGCTATCTGCATGGCGATTGCCTGACGGTGACTGGCCGCACTTTGGCCGAAAACATGGAGCACG
TTGCCTGGAATGAACACCAGGATGTGGTCCGCCCGGCCAACAGACCAATTACCCAAACCGGCGGTGTCGTGGGCTTGAAG
GGAAACCTTGCCCCCGAAGGCGCGATCGTGAAAGTCGCGGGCATGACGGAGTTGAAATTCTCCGGCCCGGCGCGCTGCTT
CGATTCGGAAGAGGAATGTTTCGAGGCGGTGACGCAACGCAACTACAGGGAAGGCGAGGTTCTCGTCATTCGCTACGAAG
GGCCGCGCGGTGGTCCCGGCATGCGCGAGATGCTGTCGACGACGGCAGCGCTCTACGGCCAGGGCATGGGCGGCAAGGTG
GCGCTGATCACCGACGGCCGCTTCTCGGGCGCGACGCGCGGCTTCTGCATCGGCCATGTCGGGCCGGAAGCGGCGGTGGG
CGGCCCGATCGGCCTCATCCGGGATGGCGACGTGATTTCGATCGACGCCGTGAACGGCACGATCGAAGTGGCGCTGTCGG
ATAGCGAACTGGCGGCGCGGGCCAAGACATGGAAGGCGCGCACGACCGACTATCAGTCGGGCGCGATATGGAAATACGCA
CAGACGGTCGGGTCTGCCAGGGATGGCGCGGTCACCCATCCGGGTGGTGCGAAAGAAACACACTGCTATGCGGATATCTG
A

Upstream 100 bases:

>100_bases
ACCCCTGCGCTAGAAGTCAGGCCGGGGAAATTGCCCATTTCCCGCCATAAACCCGGCGCACCTCGAACCTGTCTCGGGAC
ACGCGCCAAAAGGCTCGGCG

Downstream 100 bases:

>100_bases
GTTGTTGAGGTCGTCTGTCCTTCCGGCACTGGTCGCTGCGGCGATCTTTGGCGCCGTCGGCCAGGCTTTGGCCTTCGACG
ACAAGGTGTTCGACGACAAG

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD 2

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MRQAAPPAARMRMDAKVISKAKLPSRHVTVGPARAPHRSYLYAMGLSAAEIAQPLVGVASCWNEAAPCNISLMRQAQVVK
KGVAAANGTPREFCTITVTDGIAMGHQGMKSSLVSREVIADSVELTMRGHCYDALVGLAGCDKSLPGMMMAMVRLNVPSI
FIYGGSILPGSYRGRQITVQDVFEAVGQHSVGTIGDAELLEIEQAACPSAGSCGAQFTANTMATVAEAIGLALPYSCGAP
APYEMRDRFNFASGEKIMELIAKNIRPRDIITLKALENAATVVSATGGSTNAALHLPAIAHEAGIKFDLFDVARIFEKTP
YIADLKPGGKYVAKDMFEAGGIPLLMKTLLDHGYLHGDCLTVTGRTLAENMEHVAWNEHQDVVRPANRPITQTGGVVGLK
GNLAPEGAIVKVAGMTELKFSGPARCFDSEEECFEAVTQRNYREGEVLVIRYEGPRGGPGMREMLSTTAALYGQGMGGKV
ALITDGRFSGATRGFCIGHVGPEAAVGGPIGLIRDGDVISIDAVNGTIEVALSDSELAARAKTWKARTTDYQSGAIWKYA
QTVGSARDGAVTHPGGAKETHCYADI

Sequences:

>Translated_586_residues
MRQAAPPAARMRMDAKVISKAKLPSRHVTVGPARAPHRSYLYAMGLSAAEIAQPLVGVASCWNEAAPCNISLMRQAQVVK
KGVAAANGTPREFCTITVTDGIAMGHQGMKSSLVSREVIADSVELTMRGHCYDALVGLAGCDKSLPGMMMAMVRLNVPSI
FIYGGSILPGSYRGRQITVQDVFEAVGQHSVGTIGDAELLEIEQAACPSAGSCGAQFTANTMATVAEAIGLALPYSCGAP
APYEMRDRFNFASGEKIMELIAKNIRPRDIITLKALENAATVVSATGGSTNAALHLPAIAHEAGIKFDLFDVARIFEKTP
YIADLKPGGKYVAKDMFEAGGIPLLMKTLLDHGYLHGDCLTVTGRTLAENMEHVAWNEHQDVVRPANRPITQTGGVVGLK
GNLAPEGAIVKVAGMTELKFSGPARCFDSEEECFEAVTQRNYREGEVLVIRYEGPRGGPGMREMLSTTAALYGQGMGGKV
ALITDGRFSGATRGFCIGHVGPEAAVGGPIGLIRDGDVISIDAVNGTIEVALSDSELAARAKTWKARTTDYQSGAIWKYA
QTVGSARDGAVTHPGGAKETHCYADI
>Mature_586_residues
MRQAAPPAARMRMDAKVISKAKLPSRHVTVGPARAPHRSYLYAMGLSAAEIAQPLVGVASCWNEAAPCNISLMRQAQVVK
KGVAAANGTPREFCTITVTDGIAMGHQGMKSSLVSREVIADSVELTMRGHCYDALVGLAGCDKSLPGMMMAMVRLNVPSI
FIYGGSILPGSYRGRQITVQDVFEAVGQHSVGTIGDAELLEIEQAACPSAGSCGAQFTANTMATVAEAIGLALPYSCGAP
APYEMRDRFNFASGEKIMELIAKNIRPRDIITLKALENAATVVSATGGSTNAALHLPAIAHEAGIKFDLFDVARIFEKTP
YIADLKPGGKYVAKDMFEAGGIPLLMKTLLDHGYLHGDCLTVTGRTLAENMEHVAWNEHQDVVRPANRPITQTGGVVGLK
GNLAPEGAIVKVAGMTELKFSGPARCFDSEEECFEAVTQRNYREGEVLVIRYEGPRGGPGMREMLSTTAALYGQGMGGKV
ALITDGRFSGATRGFCIGHVGPEAAVGGPIGLIRDGDVISIDAVNGTIEVALSDSELAARAKTWKARTTDYQSGAIWKYA
QTVGSARDGAVTHPGGAKETHCYADI

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=606, Percent_Identity=38.4488448844884, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1786464, Length=480, Percent_Identity=35.4166666666667, Blast_Score=232, Evalue=6e-62,
Organism=Escherichia coli, GI2367371, Length=500, Percent_Identity=34.6, Blast_Score=223, Evalue=4e-59,
Organism=Escherichia coli, GI1788157, Length=506, Percent_Identity=28.2608695652174, Blast_Score=195, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6322476, Length=543, Percent_Identity=45.3038674033149, Blast_Score=460, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD2_RHILO (Q98LB3)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102764.1
- ProteinModelPortal:   Q98LB3
- SMR:   Q98LB3
- GeneID:   1225427
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll1102
- NMPDR:   fig|266835.1.peg.869
- HOGENOM:   HBG671001
- OMA:   WNEAAPC
- ProtClustDB:   PRK00911
- BRENDA:   4.2.1.9
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 61958; Mature: 61958

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQAAPPAARMRMDAKVISKAKLPSRHVTVGPARAPHRSYLYAMGLSAAEIAQPLVGVAS
CCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHEEECCCHHHHHHHHHHHHH
CWNEAAPCNISLMRQAQVVKKGVAAANGTPREFCTITVTDGIAMGHQGMKSSLVSREVIA
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHCCCHHHHHHHHHHHHH
DSVELTMRGHCYDALVGLAGCDKSLPGMMMAMVRLNVPSIFIYGGSILPGSYRGRQITVQ
CCEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCEECCCCCCCCEEEHH
DVFEAVGQHSVGTIGDAELLEIEQAACPSAGSCGAQFTANTMATVAEAIGLALPYSCGAP
HHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
APYEMRDRFNFASGEKIMELIAKNIRPRDIITLKALENAATVVSATGGSTNAALHLPAIA
CCHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCEEECCHHH
HEAGIKFDLFDVARIFEKTPYIADLKPGGKYVAKDMFEAGGIPLLMKTLLDHGYLHGDCL
HHCCCEEEHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEE
TVTGRTLAENMEHVAWNEHQDVVRPANRPITQTGGVVGLKGNLAPEGAIVKVAGMTELKF
EECCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCEEEEC
SGPARCFDSEEECFEAVTQRNYREGEVLVIRYEGPRGGPGMREMLSTTAALYGQGMGGKV
CCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEE
ALITDGRFSGATRGFCIGHVGPEAAVGGPIGLIRDGDVISIDAVNGTIEVALSDSELAAR
EEEECCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEEEECCCEEEEEECCHHHHHH
AKTWKARTTDYQSGAIWKYAQTVGSARDGAVTHPGGAKETHCYADI
HHHCCCCCCCCCCCCHHHHHHHHCCCCCCCEECCCCCCCCEEEECC
>Mature Secondary Structure
MRQAAPPAARMRMDAKVISKAKLPSRHVTVGPARAPHRSYLYAMGLSAAEIAQPLVGVAS
CCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHEEECCCHHHHHHHHHHHHH
CWNEAAPCNISLMRQAQVVKKGVAAANGTPREFCTITVTDGIAMGHQGMKSSLVSREVIA
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHCCCHHHHHHHHHHHHH
DSVELTMRGHCYDALVGLAGCDKSLPGMMMAMVRLNVPSIFIYGGSILPGSYRGRQITVQ
CCEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCEECCCCCCCCEEEHH
DVFEAVGQHSVGTIGDAELLEIEQAACPSAGSCGAQFTANTMATVAEAIGLALPYSCGAP
HHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
APYEMRDRFNFASGEKIMELIAKNIRPRDIITLKALENAATVVSATGGSTNAALHLPAIA
CCHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCEEECCHHH
HEAGIKFDLFDVARIFEKTPYIADLKPGGKYVAKDMFEAGGIPLLMKTLLDHGYLHGDCL
HHCCCEEEHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEE
TVTGRTLAENMEHVAWNEHQDVVRPANRPITQTGGVVGLKGNLAPEGAIVKVAGMTELKF
EECCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCEEEEC
SGPARCFDSEEECFEAVTQRNYREGEVLVIRYEGPRGGPGMREMLSTTAALYGQGMGGKV
CCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEE
ALITDGRFSGATRGFCIGHVGPEAAVGGPIGLIRDGDVISIDAVNGTIEVALSDSELAAR
EEEECCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEEEECCCEEEEEECCHHHHHH
AKTWKARTTDYQSGAIWKYAQTVGSARDGAVTHPGGAKETHCYADI
HHHCCCCCCCCCCCCHHHHHHHHCCCCCCCEECCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968