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The map label for this gene is glpK

Identifier: 13470885

GI number: 13470885

Start: 553001

End: 554491

Strand: Reverse

Name: glpK

Synonym: mll0700

Alternate gene names: 13470885

Gene position: 554491-553001 (Counterclockwise)

Preceding gene: 13470886

Following gene: 13470882

Centisome position: 7.88

GC content: 63.31

Gene sequence:

>1491_bases
ATGAGCGGTTTTGTGCTGGCAATCGACCAGGGAACGACATCGACCAGGGCGATCCTATTCGATGGCCAGATGAAAGTCGC
CGGCAGCGGCCAGAAGGAATTCGCGCAGCACTATCCGGCCTCCGGCTGGGTCGAGCATGACCCGGAAGAGATTTGGGCGA
GTGTTGTGACGACCGTGAAGGCTGCGCTGAAGAATGCCGGCAAAGCCGCGTCGGATGTCGCCGCCATCGGCATCACCAAT
CAGCGCGAGACCGTCGTCGTCTGGGACAGGGCGACGGGCAAGCCGATCCACAATGCCATCGTCTGGCAGGACCGCCGCAC
CGCGCCGCTCTGCCAGAAACTGAAGAAGCAGGGGCTGGAGAAGAAATTCACGCGCAAGACCGGCTTGCTGCTCGATCCCT
ATTTCTCGGGCACCAAGATCGCCTGGATACTCGACAAGGTGAAGGGCGGCAGGAAGCGCGCCGAGAAAGGCGAATTGCTG
GCCGGCACCATCGACAGTTTTCTGATCTGGCGGCTGACCGGCGGCAAGGTCCACGCCACCGACGCCACCAACGCCTCGCG
CACGCTGGTCTACAACATCGCGGAAAACGCCTGGGACGACGAGCTTTTGGCCATTCTCAACATTCCGGCGAAACTGCTGC
CGCAGGTCAAGGACTGCGCCGATGACTATGGAGTGACCGAGAGAGATCTGTTCGGTGCCGAGATAAGGATCCTCGGCGTT
GCCGGTGACCAGCATGCCGCGACCATCGGCCAGGCCTGCTTCGAGCCGGGCATGATGAAATCCACCTATGGCACCGGCTG
CTTCGCGCTGCTCAACACCGGCAGCGATCTGGTGCGTTCGAAGAACCGGCTTTTGACGACCATCGCCTATCGGCTGAACG
GCAAGACCACCTATGCGCTGGAAGGTTCGATCTTCATCGCAGGGGCTGCCGTGCAATGGCTGCGCGACGGCATCAAGGTG
ATCGGCAAGGCCGAGCAGAGCGGCAAACTGGCTGATGAGGCCGACCCGACGCAAAACGTCTATCTGGTGCCGGCTTTCGT
CGGCCTCGGCGCGCCGCACTGGGATGCCGAGGCGCGCGGTGCCATCTTCGGACTGACCCGCAATTCCGGACCGGCAGAAT
TTGCCCGCGCCGCTCTGGAGGCCGTTGCCTACCAGACCCGCGACCTGCTCGACGCCATGCGCAAGGACTGGAAGGGCACT
TCGGCCAAAACGGTGCTGAGGGTCGATGGCGGCATGGTGGCGTCGGATTGGACCATGCAGCGGCTGGCCGACATACTCGA
CGCGCCGGTCGATCGCCCGACCATCCTTGAGACGACCGCGCTGGGCGCGGCCTGGCTTGCCGGCTCGAAGGCTGGTGTCT
GGCCCAAGGCGAAGGAATTCGCCAAGAGCTGGGCGCTTGAGCGGCGGTTCAAGCCGGAGATGGATATCGCCACACGATCC
GCCAAGCTGGCAGGCTGGCGCGATGCTGTGCGCAGGACGCTAAGCGCATAA

Upstream 100 bases:

>100_bases
GCAGAAGTAAGACTGTCCTATGCGGGCGCTCGCGCCCGTGTGAAATTGGGGGAGGCGGCATGTTGCCGTCTCCCCTGTTT
GTGTGAAGGCGGAGGACGCA

Downstream 100 bases:

>100_bases
GAGAGACCCGCGCCGTTTCCGACGCGGGCCTTGTACTCAGCTGCCGATACCTGGGGTCAGTAAGGCGAACGGCACTCCTG
ACGCGGTCCGTAGTTCGGCT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSGFVLAIDQGTTSTRAILFDGQMKVAGSGQKEFAQHYPASGWVEHDPEEIWASVVTTVKAALKNAGKAASDVAAIGITN
QRETVVVWDRATGKPIHNAIVWQDRRTAPLCQKLKKQGLEKKFTRKTGLLLDPYFSGTKIAWILDKVKGGRKRAEKGELL
AGTIDSFLIWRLTGGKVHATDATNASRTLVYNIAENAWDDELLAILNIPAKLLPQVKDCADDYGVTERDLFGAEIRILGV
AGDQHAATIGQACFEPGMMKSTYGTGCFALLNTGSDLVRSKNRLLTTIAYRLNGKTTYALEGSIFIAGAAVQWLRDGIKV
IGKAEQSGKLADEADPTQNVYLVPAFVGLGAPHWDAEARGAIFGLTRNSGPAEFARAALEAVAYQTRDLLDAMRKDWKGT
SAKTVLRVDGGMVASDWTMQRLADILDAPVDRPTILETTALGAAWLAGSKAGVWPKAKEFAKSWALERRFKPEMDIATRS
AKLAGWRDAVRRTLSA

Sequences:

>Translated_496_residues
MSGFVLAIDQGTTSTRAILFDGQMKVAGSGQKEFAQHYPASGWVEHDPEEIWASVVTTVKAALKNAGKAASDVAAIGITN
QRETVVVWDRATGKPIHNAIVWQDRRTAPLCQKLKKQGLEKKFTRKTGLLLDPYFSGTKIAWILDKVKGGRKRAEKGELL
AGTIDSFLIWRLTGGKVHATDATNASRTLVYNIAENAWDDELLAILNIPAKLLPQVKDCADDYGVTERDLFGAEIRILGV
AGDQHAATIGQACFEPGMMKSTYGTGCFALLNTGSDLVRSKNRLLTTIAYRLNGKTTYALEGSIFIAGAAVQWLRDGIKV
IGKAEQSGKLADEADPTQNVYLVPAFVGLGAPHWDAEARGAIFGLTRNSGPAEFARAALEAVAYQTRDLLDAMRKDWKGT
SAKTVLRVDGGMVASDWTMQRLADILDAPVDRPTILETTALGAAWLAGSKAGVWPKAKEFAKSWALERRFKPEMDIATRS
AKLAGWRDAVRRTLSA
>Mature_495_residues
SGFVLAIDQGTTSTRAILFDGQMKVAGSGQKEFAQHYPASGWVEHDPEEIWASVVTTVKAALKNAGKAASDVAAIGITNQ
RETVVVWDRATGKPIHNAIVWQDRRTAPLCQKLKKQGLEKKFTRKTGLLLDPYFSGTKIAWILDKVKGGRKRAEKGELLA
GTIDSFLIWRLTGGKVHATDATNASRTLVYNIAENAWDDELLAILNIPAKLLPQVKDCADDYGVTERDLFGAEIRILGVA
GDQHAATIGQACFEPGMMKSTYGTGCFALLNTGSDLVRSKNRLLTTIAYRLNGKTTYALEGSIFIAGAAVQWLRDGIKVI
GKAEQSGKLADEADPTQNVYLVPAFVGLGAPHWDAEARGAIFGLTRNSGPAEFARAALEAVAYQTRDLLDAMRKDWKGTS
AKTVLRVDGGMVASDWTMQRLADILDAPVDRPTILETTALGAAWLAGSKAGVWPKAKEFAKSWALERRFKPEMDIATRSA
KLAGWRDAVRRTLSA

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=504, Percent_Identity=43.0555555555556, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI4504007, Length=504, Percent_Identity=43.0555555555556, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI42794763, Length=509, Percent_Identity=43.0255402750491, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI41393575, Length=504, Percent_Identity=42.4603174603175, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI88196792, Length=509, Percent_Identity=31.434184675835, Blast_Score=293, Evalue=3e-79,
Organism=Escherichia coli, GI1790361, Length=493, Percent_Identity=51.9269776876268, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1789987, Length=502, Percent_Identity=25.4980079681275, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1790005, Length=500, Percent_Identity=24.4, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787789, Length=484, Percent_Identity=24.3801652892562, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535599, Length=496, Percent_Identity=38.9112903225806, Blast_Score=347, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI71986140, Length=518, Percent_Identity=23.7451737451737, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI71986147, Length=300, Percent_Identity=25.3333333333333, Blast_Score=100, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321755, Length=473, Percent_Identity=37.2093023255814, Blast_Score=273, Evalue=6e-74,
Organism=Drosophila melanogaster, GI17864214, Length=508, Percent_Identity=41.9291338582677, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655555, Length=506, Percent_Identity=37.1541501976285, Blast_Score=337, Evalue=9e-93,
Organism=Drosophila melanogaster, GI24655560, Length=506, Percent_Identity=37.1541501976285, Blast_Score=337, Evalue=9e-93,
Organism=Drosophila melanogaster, GI24655570, Length=506, Percent_Identity=37.1541501976285, Blast_Score=337, Evalue=9e-93,
Organism=Drosophila melanogaster, GI24655574, Length=506, Percent_Identity=37.1541501976285, Blast_Score=337, Evalue=9e-93,
Organism=Drosophila melanogaster, GI24655565, Length=506, Percent_Identity=37.1541501976285, Blast_Score=337, Evalue=9e-93,
Organism=Drosophila melanogaster, GI24654573, Length=461, Percent_Identity=41.8655097613883, Blast_Score=331, Evalue=7e-91,
Organism=Drosophila melanogaster, GI19922058, Length=524, Percent_Identity=31.4885496183206, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=32.3828920570265, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24656367, Length=513, Percent_Identity=28.2651072124756, Blast_Score=194, Evalue=2e-49,
Organism=Drosophila melanogaster, GI21357867, Length=529, Percent_Identity=27.4102079395085, Blast_Score=192, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24656372, Length=394, Percent_Identity=27.4111675126904, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI45552909, Length=327, Percent_Identity=26.605504587156, Blast_Score=99, Evalue=9e-21,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_RHILO (Q98M73)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102454.1
- ProteinModelPortal:   Q98M73
- SMR:   Q98M73
- GeneID:   1225117
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll0700
- NMPDR:   fig|266835.1.peg.559
- HOGENOM:   HBG511469
- OMA:   GWVEHEP
- ProtClustDB:   CLSK863056
- BRENDA:   2.7.1.30
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 53804; Mature: 53673

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 12-12 BINDING 16-16 BINDING 82-82 BINDING 134-134 BINDING 243-243 BINDING 265-265 BINDING 308-308

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFVLAIDQGTTSTRAILFDGQMKVAGSGQKEFAQHYPASGWVEHDPEEIWASVVTTVK
CCCEEEEEECCCCCCEEEEEECEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
AALKNAGKAASDVAAIGITNQRETVVVWDRATGKPIHNAIVWQDRRTAPLCQKLKKQGLE
HHHHHCCCCHHCEEEEEECCCCCEEEEEECCCCCCHHHHEEECCCCCCHHHHHHHHCCHH
KKFTRKTGLLLDPYFSGTKIAWILDKVKGGRKRAEKGELLAGTIDSFLIWRLTGGKVHAT
HHHHHHCCCEECCCCCCCEEEEEEHHHCCCHHHCCCCCEEHCCCCCEEEEEEECCEEEEC
DATNASRTLVYNIAENAWDDELLAILNIPAKLLPQVKDCADDYGVTERDLFGAEIRILGV
CCCCCCCEEEEEECCCCCCHHHHHHHCCCHHHCCHHHHHHHHCCCCCHHCCCCEEEEEEE
AGDQHAATIGQACFEPGMMKSTYGTGCFALLNTGSDLVRSKNRLLTTIAYRLNGKTTYAL
CCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHCCCCEEEEEEEEECCCEEEEE
EGSIFIAGAAVQWLRDGIKVIGKAEQSGKLADEADPTQNVYLVPAFVGLGAPHWDAEARG
ECCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCCCCC
AIFGLTRNSGPAEFARAALEAVAYQTRDLLDAMRKDWKGTSAKTVLRVDGGMVASDWTMQ
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEECCHHHH
RLADILDAPVDRPTILETTALGAAWLAGSKAGVWPKAKEFAKSWALERRFKPEMDIATRS
HHHHHHHCCCCCCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH
AKLAGWRDAVRRTLSA
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGFVLAIDQGTTSTRAILFDGQMKVAGSGQKEFAQHYPASGWVEHDPEEIWASVVTTVK
CCEEEEEECCCCCCEEEEEECEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
AALKNAGKAASDVAAIGITNQRETVVVWDRATGKPIHNAIVWQDRRTAPLCQKLKKQGLE
HHHHHCCCCHHCEEEEEECCCCCEEEEEECCCCCCHHHHEEECCCCCCHHHHHHHHCCHH
KKFTRKTGLLLDPYFSGTKIAWILDKVKGGRKRAEKGELLAGTIDSFLIWRLTGGKVHAT
HHHHHHCCCEECCCCCCCEEEEEEHHHCCCHHHCCCCCEEHCCCCCEEEEEEECCEEEEC
DATNASRTLVYNIAENAWDDELLAILNIPAKLLPQVKDCADDYGVTERDLFGAEIRILGV
CCCCCCCEEEEEECCCCCCHHHHHHHCCCHHHCCHHHHHHHHCCCCCHHCCCCEEEEEEE
AGDQHAATIGQACFEPGMMKSTYGTGCFALLNTGSDLVRSKNRLLTTIAYRLNGKTTYAL
CCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHCCCCEEEEEEEEECCCEEEEE
EGSIFIAGAAVQWLRDGIKVIGKAEQSGKLADEADPTQNVYLVPAFVGLGAPHWDAEARG
ECCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCCCCC
AIFGLTRNSGPAEFARAALEAVAYQTRDLLDAMRKDWKGTSAKTVLRVDGGMVASDWTMQ
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEECCHHHH
RLADILDAPVDRPTILETTALGAAWLAGSKAGVWPKAKEFAKSWALERRFKPEMDIATRS
HHHHHHHCCCCCCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH
AKLAGWRDAVRRTLSA
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968