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The map label for this gene is rhtB [C]

Identifier: 13470847

GI number: 13470847

Start: 514433

End: 515044

Strand: Reverse

Name: rhtB [C]

Synonym: mll0653

Alternate gene names: 13470847

Gene position: 515044-514433 (Counterclockwise)

Preceding gene: 13470848

Following gene: 13470846

Centisome position: 7.32

GC content: 63.56

Gene sequence:

>612_bases
ATGACACCAACGGCATTTCTGGCTTATTGCGCCGCCGTCACACTCGCCGCCGCCACGCCGGGGCCTGCGATGTTCACGGT
CATCACCAATGGTGTGTCGCGCGGTTTCCTTCGCGCCTTCATGGCCGGTGTCGGGGTTGCCGCCGGCGATGCCGTGCTGG
TCACTCTGGCGCTGCTCGGCCTTGTGGCGCTGGCGCAAACATTCGAATGGATATTTCTCCTGCTGAAATATGCAGGCGCC
GCCTATCTGATCTTTCTCGGCATCAGGATGTGGCGCGCCTCGGCCACGCAATCGAATGAGCCGCGGACGGGTGAGGCGAG
ATTGTCCCGGTCCTTCTTCCTCGGCGCGTCCATTGCGCTGGGCAACCCCAAGGCGATCCTGTTCCACGCCTCGATCATGC
CGCTGATCCTCAACCTCGACACGCTGACATTCGTCGATGGCCTGCTGGTGGTGGCCACCGTCATCAGCGTCAATATCCTG
ACCATGGGGGTCTACGCAGCCCTTGCGGGCCGGGCTTCGGGCTGGTTCAGGACGCCGAAGCGCATGCGGCTGATGAACCG
GTTTGCCGGCAGCGCCATGATCGGCACCGGAGCGCTGATTGCCGCACGCTGA

Upstream 100 bases:

>100_bases
AGCGCGACCGCGCGGTGCGGCTCGGCGCCATCGAATGCAGCCGCGAGGAAATCGTCGCGATCTTCCGGCGCGTGCGGGTG
CCCAATGGAAAGGCCAAGCG

Downstream 100 bases:

>100_bases
GCGGTAAAACACCGCCCCGCTTGCGTTTGTCGCGCTTCCCCTCTATATGCGCGCTCATTCCACACACGGACTTTGGTGTC
TGCCCGGGAGAAATCCGGCT

Product: hypothetical protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MTPTAFLAYCAAVTLAAATPGPAMFTVITNGVSRGFLRAFMAGVGVAAGDAVLVTLALLGLVALAQTFEWIFLLLKYAGA
AYLIFLGIRMWRASATQSNEPRTGEARLSRSFFLGASIALGNPKAILFHASIMPLILNLDTLTFVDGLLVVATVISVNIL
TMGVYAALAGRASGWFRTPKRMRLMNRFAGSAMIGTGALIAAR

Sequences:

>Translated_203_residues
MTPTAFLAYCAAVTLAAATPGPAMFTVITNGVSRGFLRAFMAGVGVAAGDAVLVTLALLGLVALAQTFEWIFLLLKYAGA
AYLIFLGIRMWRASATQSNEPRTGEARLSRSFFLGASIALGNPKAILFHASIMPLILNLDTLTFVDGLLVVATVISVNIL
TMGVYAALAGRASGWFRTPKRMRLMNRFAGSAMIGTGALIAAR
>Mature_202_residues
TPTAFLAYCAAVTLAAATPGPAMFTVITNGVSRGFLRAFMAGVGVAAGDAVLVTLALLGLVALAQTFEWIFLLLKYAGAA
YLIFLGIRMWRASATQSNEPRTGEARLSRSFFLGASIALGNPKAILFHASIMPLILNLDTLTFVDGLLVVATVISVNILT
MGVYAALAGRASGWFRTPKRMRLMNRFAGSAMIGTGALIAAR

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=202, Percent_Identity=30.6930693069307, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1788099, Length=190, Percent_Identity=31.0526315789474, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI48994978, Length=134, Percent_Identity=32.8358208955224, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21378; Mature: 21247

Theoretical pI: Translated: 11.62; Mature: 11.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTAFLAYCAAVTLAAATPGPAMFTVITNGVSRGFLRAFMAGVGVAAGDAVLVTLALLG
CCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LVALAQTFEWIFLLLKYAGAAYLIFLGIRMWRASATQSNEPRTGEARLSRSFFLGASIAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEC
GNPKAILFHASIMPLILNLDTLTFVDGLLVVATVISVNILTMGVYAALAGRASGWFRTPK
CCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
RMRLMNRFAGSAMIGTGALIAAR
HHHHHHHHCCCHHCCCCEEEECC
>Mature Secondary Structure 
TPTAFLAYCAAVTLAAATPGPAMFTVITNGVSRGFLRAFMAGVGVAAGDAVLVTLALLG
CHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LVALAQTFEWIFLLLKYAGAAYLIFLGIRMWRASATQSNEPRTGEARLSRSFFLGASIAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEC
GNPKAILFHASIMPLILNLDTLTFVDGLLVVATVISVNILTMGVYAALAGRASGWFRTPK
CCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
RMRLMNRFAGSAMIGTGALIAAR
HHHHHHHHCCCHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]