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The map label for this gene is ispDF

Identifier: 13470629

GI number: 13470629

Start: 279544

End: 280767

Strand: Reverse

Name: ispDF

Synonym: mll0395

Alternate gene names: 13470629

Gene position: 280767-279544 (Counterclockwise)

Preceding gene: 13470638

Following gene: 13470628

Centisome position: 3.99

GC content: 65.69

Gene sequence:

>1224_bases
ATGACTGAGGCAAGTGAAAACGCGAGCGCGACAGGTGGGGTTGCGGTGGTGATCGTCGCCGCCGGCCGCGGCGCTCGTGC
CGGGCAGGCCAACGGACCCAAGCAATACCAGAACATTGGCGGCCGCGCCGTCATTGCGCACACGCTGGAGATATTTCTGG
CCCATCCGCGAACCGACCGGATCGTCGTCGCCATCCACGCCGACGACCACGAATTGTTCCGGCAAGCGGCGGGCAGCCAG
GCTGAGCGCGTCACCGCCATCATTGGCGGACCGACCCGGCAGGAGTCCGTCCGGCTCGGGCTGCTGGCGCTGAAGGAGCA
TGCGCCGGGCCAGGTTCTAATTCACGACGCCGTCCGCCCGTTCGTCGATGCGGACCTGATCGACCGAACCATTTCCGCCA
TCGGCGAGAACGAGGGGGCGTTGCCCGCCCTCCCCGTCGCAGACACGCTGAAGCGCGAATCGGCCGCCGGAGTGGTCGCG
GAAACCGTTTCCCGAAGCGGGCTGCACGCGGCGCAAACGCCGCAAGGTTTTCCCTACGGGCCGATCCTTGCCGCACACGA
CAAGGCCTTTCAACTGGGTAGGCTGGACTTCACTGATGACGCGGCCATCGCCGAATGGGCGCATATTCCAGTCAAGCTCG
TTCCGGGCTCGCCGGACAATGTCAAACTCACCTGGGCACGGGACATCGCCATGGCGCACCAGCGGCTTTCCAGCGAACGC
ACACATTTCCCCGACATCCGCACCGGCAACGGCTACGATGTCCACGCCTTTGAGCCCGGCGATCACGTCACTTTGTGCGG
CGTCGCCATTCCACATGACAAGAAACTGTCCGGTCATTCCGATGCCGATGTCGGCCTGCATGCCTTGACCGACGCGCTGC
TGGCGACCTGCGGAGCCGGTGACATCGGTACGCATTTTCCGCCGTCCGATCCGCAGTGGAAAGGTGCGGCGTCGCGGATC
TTCGTCGAACACGCGGCGAAAGTCGTGCGCCAGCGCGGCGGACGCATCGCCAATGCCGACATCACGCTGATCTGCGAGGC
GCCGCGCGTCGGCCCGCATCGCGAGGCGATGACGGCAGCCCTGTCGCGGATGCTGGGCATTTCCGCCGACCGCATCTCGA
TCAAGGCGACAACCAACGAAAAACTCGGCTTCGTCGGCCGCGAGGAAGGCATTGCGGCGATCGCCACCGCCAGCGTGGTG
TTCCCCGGCGAGGTGCCGGAATGA

Upstream 100 bases:

>100_bases
CCTCAGGGCATGTGAAGGATGCTTTGGAATTCACATTTGACGCACGATCTTTTTGGAAAACCAATTACACTTTTGGGGAT
CGTGCGCTAAGCCAATCGCC

Downstream 100 bases:

>100_bases
GCAACGCCGAACTCGCAAACTCCCTGCTGCTGGCCTGCCAGCAACGCGGCATCATGCTGGCGACGGCTGAAAGCTGCACC
GGCGGCCTGATCATCGCCGC

Product: bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase protein

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MTEASENASATGGVAVVIVAAGRGARAGQANGPKQYQNIGGRAVIAHTLEIFLAHPRTDRIVVAIHADDHELFRQAAGSQ
AERVTAIIGGPTRQESVRLGLLALKEHAPGQVLIHDAVRPFVDADLIDRTISAIGENEGALPALPVADTLKRESAAGVVA
ETVSRSGLHAAQTPQGFPYGPILAAHDKAFQLGRLDFTDDAAIAEWAHIPVKLVPGSPDNVKLTWARDIAMAHQRLSSER
THFPDIRTGNGYDVHAFEPGDHVTLCGVAIPHDKKLSGHSDADVGLHALTDALLATCGAGDIGTHFPPSDPQWKGAASRI
FVEHAAKVVRQRGGRIANADITLICEAPRVGPHREAMTAALSRMLGISADRISIKATTNEKLGFVGREEGIAAIATASVV
FPGEVPE

Sequences:

>Translated_407_residues
MTEASENASATGGVAVVIVAAGRGARAGQANGPKQYQNIGGRAVIAHTLEIFLAHPRTDRIVVAIHADDHELFRQAAGSQ
AERVTAIIGGPTRQESVRLGLLALKEHAPGQVLIHDAVRPFVDADLIDRTISAIGENEGALPALPVADTLKRESAAGVVA
ETVSRSGLHAAQTPQGFPYGPILAAHDKAFQLGRLDFTDDAAIAEWAHIPVKLVPGSPDNVKLTWARDIAMAHQRLSSER
THFPDIRTGNGYDVHAFEPGDHVTLCGVAIPHDKKLSGHSDADVGLHALTDALLATCGAGDIGTHFPPSDPQWKGAASRI
FVEHAAKVVRQRGGRIANADITLICEAPRVGPHREAMTAALSRMLGISADRISIKATTNEKLGFVGREEGIAAIATASVV
FPGEVPE
>Mature_406_residues
TEASENASATGGVAVVIVAAGRGARAGQANGPKQYQNIGGRAVIAHTLEIFLAHPRTDRIVVAIHADDHELFRQAAGSQA
ERVTAIIGGPTRQESVRLGLLALKEHAPGQVLIHDAVRPFVDADLIDRTISAIGENEGALPALPVADTLKRESAAGVVAE
TVSRSGLHAAQTPQGFPYGPILAAHDKAFQLGRLDFTDDAAIAEWAHIPVKLVPGSPDNVKLTWARDIAMAHQRLSSERT
HFPDIRTGNGYDVHAFEPGDHVTLCGVAIPHDKKLSGHSDADVGLHALTDALLATCGAGDIGTHFPPSDPQWKGAASRIF
VEHAAKVVRQRGGRIANADITLICEAPRVGPHREAMTAALSRMLGISADRISIKATTNEKLGFVGREEGIAAIATASVVF
PGEVPE

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family

Homologues:

Organism=Homo sapiens, GI157412259, Length=260, Percent_Identity=30.7692307692308, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1789103, Length=155, Percent_Identity=46.4516129032258, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1789104, Length=227, Percent_Identity=37.4449339207048, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPDF_RHILO (Q98MX9)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102198.1
- ProteinModelPortal:   Q98MX9
- SMR:   Q98MX9
- GeneID:   1224861
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll0395
- NMPDR:   fig|266835.1.peg.303
- HOGENOM:   HBG672839
- OMA:   IVLIHDA
- ProtClustDB:   PRK09382
- BRENDA:   2.7.7.60
- BRENDA:   4.6.1.12
- HAMAP:   MF_01520
- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00453
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF01128 IspD; PF02542 YgbB; SSF69765 YgbB_synth

EC number: =2.7.7.60; =4.6.1.12

Molecular weight: Translated: 42835; Mature: 42704

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS01295 ISPD; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEASENASATGGVAVVIVAAGRGARAGQANGPKQYQNIGGRAVIAHTLEIFLAHPRTDR
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHCCCEEEHHEEEEHHEECCCCCE
IVVAIHADDHELFRQAAGSQAERVTAIIGGPTRQESVRLGLLALKEHAPGQVLIHDAVRP
EEEEEECCCHHHHHHHCCCCHHEEEEEECCCCCCHHHEEEEEEECCCCCCEEEEHHHHHH
FVDADLIDRTISAIGENEGALPALPVADTLKRESAAGVVAETVSRSGLHAAQTPQGFPYG
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCC
PILAAHDKAFQLGRLDFTDDAAIAEWAHIPVKLVPGSPDNVKLTWARDIAMAHQRLSSER
CEEECCCCCEEECCCCCCCCHHHHHHHCCCEEEECCCCCCEEEEEHHHHHHHHHHHHCCC
THFPDIRTGNGYDVHAFEPGDHVTLCGVAIPHDKKLSGHSDADVGLHALTDALLATCGAG
CCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
DIGTHFPPSDPQWKGAASRIFVEHAAKVVRQRGGRIANADITLICEAPRVGPHREAMTAA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCEEEEECCCCCCCHHHHHHHH
LSRMLGISADRISIKATTNEKLGFVGREEGIAAIATASVVFPGEVPE
HHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEEEECCCCCCC
>Mature Secondary Structure 
TEASENASATGGVAVVIVAAGRGARAGQANGPKQYQNIGGRAVIAHTLEIFLAHPRTDR
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHCCCEEEHHEEEEHHEECCCCCE
IVVAIHADDHELFRQAAGSQAERVTAIIGGPTRQESVRLGLLALKEHAPGQVLIHDAVRP
EEEEEECCCHHHHHHHCCCCHHEEEEEECCCCCCHHHEEEEEEECCCCCCEEEEHHHHHH
FVDADLIDRTISAIGENEGALPALPVADTLKRESAAGVVAETVSRSGLHAAQTPQGFPYG
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCC
PILAAHDKAFQLGRLDFTDDAAIAEWAHIPVKLVPGSPDNVKLTWARDIAMAHQRLSSER
CEEECCCCCEEECCCCCCCCHHHHHHHCCCEEEECCCCCCEEEEEHHHHHHHHHHHHCCC
THFPDIRTGNGYDVHAFEPGDHVTLCGVAIPHDKKLSGHSDADVGLHALTDALLATCGAG
CCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
DIGTHFPPSDPQWKGAASRIFVEHAAKVVRQRGGRIANADITLICEAPRVGPHREAMTAA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCEEEEECCCCCCCHHHHHHHH
LSRMLGISADRISIKATTNEKLGFVGREEGIAAIATASVVFPGEVPE
HHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968