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The map label for this gene is alaXM [H]

Identifier: 13470598

GI number: 13470598

Start: 250634

End: 251380

Strand: Direct

Name: alaXM [H]

Synonym: mlr0354

Alternate gene names: 13470598

Gene position: 250634-251380 (Clockwise)

Preceding gene: 13470597

Following gene: 13470599

Centisome position: 3.56

GC content: 61.31

Gene sequence:

>747_bases
ATGATGGCGCACAAGACGGAAGCGTTGTTTCGCGACGATGCCTATCTCGCCACGGCGGATGCGGCGGTTGCTGCCGTCAA
TGATCGTGGCGGCATCATTTTCGATCGGACGATCTTCTACGCCACCTCGGGTGGCCAGCCGGGCGATACCGGCCATCTCG
AACGTGCCGACGGCAGCCGCATCGCCATTGCCGCCACCATCACAGGTGAGACCAAGGACGAGATCATCCACGTACCGGCG
CCGCAGCAGGCGGCCCTGGCGGTTGGCGAGAAGGTCAAACTCGCCATCGACTGGGAACGTCGGCACCTGTTGATGCGCAT
GCACGCGGCTTGCCATCTGCTTACCGTCGTTTGTCCGTTTCCAATCACCGGTGCGGCGGTTTCCGAGGACGATAGCCGTG
TAGATTTCGACATTCCGGACGCCAGCTTCTCGAAAGAAGAGGTGACCGCGAGCCTGATGGATCTGGTGCGCGCCAATCAT
CCGATTTTCACCAGGCTGATCAGCGATGAGGAACTCGCCGCTAACCCCGGCCTGGTGAAGTCAAAAAATGTCCGGCCGCC
GGTCGGCACCGGCAAGATACGCCTGGTTTGCATTGGTGAGAATGGCGCGGTCGATAGCCAGCCCTGTGGTGGCACACATG
TGAAAAGCACCGGCGAGATCGGCGAAATCCACATCGGCAAGATCGAGAAGAAGGGCCGCGAGAACAGGCGCTTCCGCATC
CGCTTCGGCCCGATGCCGGCAGCCTGA

Upstream 100 bases:

>100_bases
CGAAGCTGTTCAATCCGGAATTCCTGCGCGAGAAGAATCTCCCGGTGCCGGGGTGGATGGAACTGCAGAGCAAGATTTCG
GTGCCTTTCGAAAAGGTCGA

Downstream 100 bases:

>100_bases
ACACGCAACACTTGCTTACCCGGGATAGAGAGAACCAGAATGGCCCAGGACAGTCCTTTCACCGTCGATGCGGACTGGCT
GCAGGCGCGCCTGGGCGAGC

Product: alanyl-tRNA synthetase

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; AlaXp; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MMAHKTEALFRDDAYLATADAAVAAVNDRGGIIFDRTIFYATSGGQPGDTGHLERADGSRIAIAATITGETKDEIIHVPA
PQQAALAVGEKVKLAIDWERRHLLMRMHAACHLLTVVCPFPITGAAVSEDDSRVDFDIPDASFSKEEVTASLMDLVRANH
PIFTRLISDEELAANPGLVKSKNVRPPVGTGKIRLVCIGENGAVDSQPCGGTHVKSTGEIGEIHIGKIEKKGRENRRFRI
RFGPMPAA

Sequences:

>Translated_248_residues
MMAHKTEALFRDDAYLATADAAVAAVNDRGGIIFDRTIFYATSGGQPGDTGHLERADGSRIAIAATITGETKDEIIHVPA
PQQAALAVGEKVKLAIDWERRHLLMRMHAACHLLTVVCPFPITGAAVSEDDSRVDFDIPDASFSKEEVTASLMDLVRANH
PIFTRLISDEELAANPGLVKSKNVRPPVGTGKIRLVCIGENGAVDSQPCGGTHVKSTGEIGEIHIGKIEKKGRENRRFRI
RFGPMPAA
>Mature_248_residues
MMAHKTEALFRDDAYLATADAAVAAVNDRGGIIFDRTIFYATSGGQPGDTGHLERADGSRIAIAATITGETKDEIIHVPA
PQQAALAVGEKVKLAIDWERRHLLMRMHAACHLLTVVCPFPITGAAVSEDDSRVDFDIPDASFSKEEVTASLMDLVRANH
PIFTRLISDEELAANPGLVKSKNVRPPVGTGKIRLVCIGENGAVDSQPCGGTHVKSTGEIGEIHIGKIEKKGRENRRFRI
RFGPMPAA

Specific function: Functions in trans to edit the amino acid moiety from mischarged Ser-tRNA(Ala). Recognition depends, at least in part, on the acceptor stem of tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=216, Percent_Identity=29.6296296296296, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI217416402, Length=216, Percent_Identity=29.6296296296296, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1789048, Length=230, Percent_Identity=31.304347826087, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17536681, Length=249, Percent_Identity=27.710843373494, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24582809, Length=239, Percent_Identity=27.6150627615063, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI45552267, Length=239, Percent_Identity=27.6150627615063, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 26700; Mature: 26700

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAHKTEALFRDDAYLATADAAVAAVNDRGGIIFDRTIFYATSGGQPGDTGHLERADGSR
CCCCCCHHHHCCCCEEEECCEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCEECCCCCE
IAIAATITGETKDEIIHVPAPQQAALAVGEKVKLAIDWERRHLLMRMHAACHLLTVVCPF
EEEEEEECCCCCCCEEECCCCCHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHEEEEECCC
PITGAAVSEDDSRVDFDIPDASFSKEEVTASLMDLVRANHPIFTRLISDEELAANPGLVK
CCCCCEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHCCCCCCEE
SKNVRPPVGTGKIRLVCIGENGAVDSQPCGGTHVKSTGEIGEIHIGKIEKKGRENRRFRI
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEE
RFGPMPAA
EECCCCCC
>Mature Secondary Structure
MMAHKTEALFRDDAYLATADAAVAAVNDRGGIIFDRTIFYATSGGQPGDTGHLERADGSR
CCCCCCHHHHCCCCEEEECCEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCEECCCCCE
IAIAATITGETKDEIIHVPAPQQAALAVGEKVKLAIDWERRHLLMRMHAACHLLTVVCPF
EEEEEEECCCCCCCEEECCCCCHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHEEEEECCC
PITGAAVSEDDSRVDFDIPDASFSKEEVTASLMDLVRANHPIFTRLISDEELAANPGLVK
CCCCCEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHCCCCCCEE
SKNVRPPVGTGKIRLVCIGENGAVDSQPCGGTHVKSTGEIGEIHIGKIEKKGRENRRFRI
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEE
RFGPMPAA
EECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]