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The map label for this gene is proP [H]

Identifier: 13470586

GI number: 13470586

Start: 233793

End: 235682

Strand: Direct

Name: proP [H]

Synonym: mlr0338

Alternate gene names: 13470586

Gene position: 233793-235682 (Clockwise)

Preceding gene: 13470584

Following gene: 13470587

Centisome position: 3.32

GC content: 61.85

Gene sequence:

>1890_bases
ATGGCAATGGCATCAACCGCCGGCAGAACGAGCCGCGGCATGACGCGGGAAGAGAAGAAGGTCATCTTCGCTTCCTCGCT
CGGCACCGTTTTCGAATGGTACGATTTCTATCTCTACGGTTCATTGGCGGCCTTCATTGGCTCGACCTTTTTCAGCCCGA
CCATTCCGGAGGCGACGCGCAACATCTTCGCGCTGCTGGCGTTCGCGGCCGGCTTTCTTGTGCGCCCGTTCGGGGCTCTG
GTGTTTGGCCGTATCGGCGACCTCGTCGGCCGCAAATATACCTTCCTCGTCACCATGACGATCATGGGTCTGTCGACCTT
CCTGGTCGGCTTGCTGCCCGGTTATGCGACCTTGGGTATCGCGGCTCCGGTGATCCTGATCGCCCTGCGCATGCTACAGG
GCCTGGCCCTCGGCGGCGAATATGGCGGTGCGGCGACCTACGTCGCCGAGCACGCGCCTGACGACCGTCGCGGTTTCTAC
ACGTCGTGGATCCAGACGACGGCGACGCTTGGCCTGTTCCTGTCGCTGATCGTCATTCTGATTGTTCAGGGCTCCCTGAG
CAAGGAAACCTACGCCGCCTGGGGCTGGCGCATTCCGTTCATTGTTTCTTTCGTGCTGCTCGCCGTTTCGATCTGGATTC
GGCTATCGCTTTCGGAATCGCCGACCTTCCAGAAGATGAAGGACGAAGGAAAGGGTTCGAAGGCACCGCTTTCGGAAGCC
TTCGGTCAGTGGAAGAATGCCAAGATCGCACTGCTGGCGCTGCTTGGCCTCACTGCTGGCCAGGCCGTGGTCTGGTACAA
TGGCCAGTTCTATGCGCTGTTCTTCCTGCAGAACGTGCTGAAGGTCGATGCGCAGTCGGTCAACATCATGATCGCGATCG
CGCTGGCACTCGGCTCGATCTTCTTCGTCGTGTTCGGCTGGCTCTCCGACAAGATCGGCCGCAAGCCGATCATCATGGCT
GGGCTTGCGCTGGCCATCGTCTGCACCTTCCCGCTGTTCAAGGCGTTGACCTGGGCCGCCAATCCGGCGCTCGCCACCGC
GCAGCAGAACACGCGCGCCACGGTGACCGCGGCACCCGGCGACTGCAAGTTCCAGTTCAACCCGGTCGGCACCGCCAAGT
TCACGACGTCATGCGACATCGCCACTTCGTTCCTGACCAAGAACTCGGTTCCCTTTGACGTGGTGTCGACTGCCGCGGCC
GGAACGCCTGCTACCGTCAAGATCGGCAACGAGACGGTGGCGTCCTACGACGCGGTCGCCGCTGGCGACCAAGCCAAGGC
CAAGGACGCCGCCTTCGTCAAGGCCGTCAACATGTCGCTTAAGGACGGCGGGTATCCGCTGAAGCGTGCGGCGATCAAGG
TGGCGGACCAGAAGCTCGACGCCTTCATCGCGGCCAATCCTGAACTCAAGCTCGACGCGGCTACAATTCGCGGCGGCGAG
AAGGCAACCGTGCCGACCGAGCAGGCGGTCAAGGACAAGTTGGTGACGTCAGAGGAGGCCGCCGGCGCGCCAGAGGTCAC
CGTCTACAACATACCGGGCGGCGGTGCCTTCGCCATGTTCGCCGATCCGGCCGCCGTGAACTGGCCGATGACGATCGGCA
TCCTGTTCATCCTGGTGCTGTTCGTGACAATGGTTTACGGGCCGATCGCGGCGATCCTGGTCGAGATGTTCCCGACCCGC
ATTCGTTACACCGGCATGTCGCTGCCCTATCATATCGGCAATGGCTGGTTCGGCGGTCTGTTGCCGGCAACGGTCTTCGC
GCTCAGCGCCTACAGGGGCGATATCTACTTTGGTCTGTGGTATCCGGTGGTGATCGCGGCGATGACGCTGATCATCGGGA
TGATCTTCGTCAAGGATACGCTCGGCACCGACCTCCGCGCCAAGGAGTAG

Upstream 100 bases:

>100_bases
TTTTCCCGTGATGGCCCGGCATTTCCGAGTCCGCGTTCGGAGGAGAGCGCGAACATCGGCGCCCGGCATCGAGGACAGGG
TCTCACGGGAGGACAACTCT

Downstream 100 bases:

>100_bases
CCCACGGATCATCGACCAACAGAAAGCCCGGCGTGAGCGATCACGCCGGGCTTTTTCATGTTTCAACTTGAAGAAGCGGA
TCAGCTCTTTGGCTGATTTT

Product: integral membrane sugar transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MAMASTAGRTSRGMTREEKKVIFASSLGTVFEWYDFYLYGSLAAFIGSTFFSPTIPEATRNIFALLAFAAGFLVRPFGAL
VFGRIGDLVGRKYTFLVTMTIMGLSTFLVGLLPGYATLGIAAPVILIALRMLQGLALGGEYGGAATYVAEHAPDDRRGFY
TSWIQTTATLGLFLSLIVILIVQGSLSKETYAAWGWRIPFIVSFVLLAVSIWIRLSLSESPTFQKMKDEGKGSKAPLSEA
FGQWKNAKIALLALLGLTAGQAVVWYNGQFYALFFLQNVLKVDAQSVNIMIAIALALGSIFFVVFGWLSDKIGRKPIIMA
GLALAIVCTFPLFKALTWAANPALATAQQNTRATVTAAPGDCKFQFNPVGTAKFTTSCDIATSFLTKNSVPFDVVSTAAA
GTPATVKIGNETVASYDAVAAGDQAKAKDAAFVKAVNMSLKDGGYPLKRAAIKVADQKLDAFIAANPELKLDAATIRGGE
KATVPTEQAVKDKLVTSEEAAGAPEVTVYNIPGGGAFAMFADPAAVNWPMTIGILFILVLFVTMVYGPIAAILVEMFPTR
IRYTGMSLPYHIGNGWFGGLLPATVFALSAYRGDIYFGLWYPVVIAAMTLIIGMIFVKDTLGTDLRAKE

Sequences:

>Translated_629_residues
MAMASTAGRTSRGMTREEKKVIFASSLGTVFEWYDFYLYGSLAAFIGSTFFSPTIPEATRNIFALLAFAAGFLVRPFGAL
VFGRIGDLVGRKYTFLVTMTIMGLSTFLVGLLPGYATLGIAAPVILIALRMLQGLALGGEYGGAATYVAEHAPDDRRGFY
TSWIQTTATLGLFLSLIVILIVQGSLSKETYAAWGWRIPFIVSFVLLAVSIWIRLSLSESPTFQKMKDEGKGSKAPLSEA
FGQWKNAKIALLALLGLTAGQAVVWYNGQFYALFFLQNVLKVDAQSVNIMIAIALALGSIFFVVFGWLSDKIGRKPIIMA
GLALAIVCTFPLFKALTWAANPALATAQQNTRATVTAAPGDCKFQFNPVGTAKFTTSCDIATSFLTKNSVPFDVVSTAAA
GTPATVKIGNETVASYDAVAAGDQAKAKDAAFVKAVNMSLKDGGYPLKRAAIKVADQKLDAFIAANPELKLDAATIRGGE
KATVPTEQAVKDKLVTSEEAAGAPEVTVYNIPGGGAFAMFADPAAVNWPMTIGILFILVLFVTMVYGPIAAILVEMFPTR
IRYTGMSLPYHIGNGWFGGLLPATVFALSAYRGDIYFGLWYPVVIAAMTLIIGMIFVKDTLGTDLRAKE
>Mature_628_residues
AMASTAGRTSRGMTREEKKVIFASSLGTVFEWYDFYLYGSLAAFIGSTFFSPTIPEATRNIFALLAFAAGFLVRPFGALV
FGRIGDLVGRKYTFLVTMTIMGLSTFLVGLLPGYATLGIAAPVILIALRMLQGLALGGEYGGAATYVAEHAPDDRRGFYT
SWIQTTATLGLFLSLIVILIVQGSLSKETYAAWGWRIPFIVSFVLLAVSIWIRLSLSESPTFQKMKDEGKGSKAPLSEAF
GQWKNAKIALLALLGLTAGQAVVWYNGQFYALFFLQNVLKVDAQSVNIMIAIALALGSIFFVVFGWLSDKIGRKPIIMAG
LALAIVCTFPLFKALTWAANPALATAQQNTRATVTAAPGDCKFQFNPVGTAKFTTSCDIATSFLTKNSVPFDVVSTAAAG
TPATVKIGNETVASYDAVAAGDQAKAKDAAFVKAVNMSLKDGGYPLKRAAIKVADQKLDAFIAANPELKLDAATIRGGEK
ATVPTEQAVKDKLVTSEEAAGAPEVTVYNIPGGGAFAMFADPAAVNWPMTIGILFILVLFVTMVYGPIAAILVEMFPTRI
RYTGMSLPYHIGNGWFGGLLPATVFALSAYRGDIYFGLWYPVVIAAMTLIIGMIFVKDTLGTDLRAKE

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=326, Percent_Identity=36.1963190184049, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI1788292, Length=321, Percent_Identity=33.9563862928349, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1788942, Length=328, Percent_Identity=34.7560975609756, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789941, Length=345, Percent_Identity=34.7826086956522, Blast_Score=157, Evalue=3e-39,
Organism=Escherichia coli, GI87082231, Length=226, Percent_Identity=25.2212389380531, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87082404, Length=321, Percent_Identity=25.2336448598131, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 67289; Mature: 67158

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMASTAGRTSRGMTREEKKVIFASSLGTVFEWYDFYLYGSLAAFIGSTFFSPTIPEATR
CCCCCCCCCCCCCCCHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
NIFALLAFAAGFLVRPFGALVFGRIGDLVGRKYTFLVTMTIMGLSTFLVGLLPGYATLGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AAPVILIALRMLQGLALGGEYGGAATYVAEHAPDDRRGFYTSWIQTTATLGLFLSLIVIL
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IVQGSLSKETYAAWGWRIPFIVSFVLLAVSIWIRLSLSESPTFQKMKDEGKGSKAPLSEA
HHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHCCCCCCCCHHHH
FGQWKNAKIALLALLGLTAGQAVVWYNGQFYALFFLQNVLKVDAQSVNIMIAIALALGSI
HCCCCCCHHHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FFVVFGWLSDKIGRKPIIMAGLALAIVCTFPLFKALTWAANPALATAQQNTRATVTAAPG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCEEEEECCC
DCKFQFNPVGTAKFTTSCDIATSFLTKNSVPFDVVSTAAAGTPATVKIGNETVASYDAVA
CCEEEECCCCCCEEECCHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEECCCHHHCCHHHC
AGDQAKAKDAAFVKAVNMSLKDGGYPLKRAAIKVADQKLDAFIAANPELKLDAATIRGGE
CCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCEEEEEEEECCCC
KATVPTEQAVKDKLVTSEEAAGAPEVTVYNIPGGGAFAMFADPAAVNWPMTIGILFILVL
CCCCCHHHHHHHHHCCCHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHH
FVTMVYGPIAAILVEMFPTRIRYTGMSLPYHIGNGWFGGLLPATVFALSAYRGDIYFGLW
HHHHHHHHHHHHHHHHCCHHEEEECCCCCEECCCCCCHHHHHHHHHHHHHHCCCEEEHHH
YPVVIAAMTLIIGMIFVKDTLGTDLRAKE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AMASTAGRTSRGMTREEKKVIFASSLGTVFEWYDFYLYGSLAAFIGSTFFSPTIPEATR
CCCCCCCCCCCCCCHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
NIFALLAFAAGFLVRPFGALVFGRIGDLVGRKYTFLVTMTIMGLSTFLVGLLPGYATLGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AAPVILIALRMLQGLALGGEYGGAATYVAEHAPDDRRGFYTSWIQTTATLGLFLSLIVIL
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IVQGSLSKETYAAWGWRIPFIVSFVLLAVSIWIRLSLSESPTFQKMKDEGKGSKAPLSEA
HHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHCCCCCCCCHHHH
FGQWKNAKIALLALLGLTAGQAVVWYNGQFYALFFLQNVLKVDAQSVNIMIAIALALGSI
HCCCCCCHHHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FFVVFGWLSDKIGRKPIIMAGLALAIVCTFPLFKALTWAANPALATAQQNTRATVTAAPG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCEEEEECCC
DCKFQFNPVGTAKFTTSCDIATSFLTKNSVPFDVVSTAAAGTPATVKIGNETVASYDAVA
CCEEEECCCCCCEEECCHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEECCCHHHCCHHHC
AGDQAKAKDAAFVKAVNMSLKDGGYPLKRAAIKVADQKLDAFIAANPELKLDAATIRGGE
CCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCEEEEEEEECCCC
KATVPTEQAVKDKLVTSEEAAGAPEVTVYNIPGGGAFAMFADPAAVNWPMTIGILFILVL
CCCCCHHHHHHHHHCCCHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHH
FVTMVYGPIAAILVEMFPTRIRYTGMSLPYHIGNGWFGGLLPATVFALSAYRGDIYFGLW
HHHHHHHHHHHHHHHHCCHHEEEECCCCCEECCCCCCHHHHHHHHHHHHHHCCCEEEHHH
YPVVIAAMTLIIGMIFVKDTLGTDLRAKE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]