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The map label for this gene is gloA [H]

Identifier: 13470455

GI number: 13470455

Start: 117555

End: 117995

Strand: Reverse

Name: gloA [H]

Synonym: mll0167

Alternate gene names: 13470455

Gene position: 117995-117555 (Counterclockwise)

Preceding gene: 13470459

Following gene: 13470454

Centisome position: 1.68

GC content: 61.45

Gene sequence:

>441_bases
ATGCGCTATCTGCACACCATGGTCCGCGTCGCCGACGTCGATGCCTCGCTCGATTTCTACTGCAACAAGCTCGGGCTGAA
GGAGGTCCGCCGCTACGAAAACGAGCAGGGCCGTTTCACGCTGATCTTCCTCGCCGCCTCCGAAGACGAGCAAAGCGGCA
TCAACGACAAGGCGCCGCTGATCGAACTCACCTACAACTGGGATCCGGAAGACTACAAGGGCGGGCGCAATTTCGGCCAC
CTCGCCTATGAGGTCGATGACATCTACGCCACCTGCCAGCATCTGATGGACAATGGCGTCACCATCAACCGGCCGCCGCG
CGACGGCAATATGGCCTTCATCCGGTCGCCGGACGGCATCTCCATCGAGCTTCTGCAGAAGGGCCCGGCGAAAGCCAAGG
CCGAACCCTGGGCTTCGATGGCCAACACCGGAAGCTGGTAG

Upstream 100 bases:

>100_bases
ACCGGATTTGTTCTTTGCCGGCGTCGCCGACCGCGGGAGATTGCCCTTTCGCCGGGCCGACTCTATGTTGCGCCGCACCC
CAACAGATCGAGGAAAACTC

Downstream 100 bases:

>100_bases
CAATCACGCCATCTTGAAGCGAGGCGGCCGGGGTAAAAGCCGGCCGCCTTTGCCGTTTCGCACCACGCTACCTATCTGAC
CGGTCATCACGCCTGGCCGG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MRYLHTMVRVADVDASLDFYCNKLGLKEVRRYENEQGRFTLIFLAASEDEQSGINDKAPLIELTYNWDPEDYKGGRNFGH
LAYEVDDIYATCQHLMDNGVTINRPPRDGNMAFIRSPDGISIELLQKGPAKAKAEPWASMANTGSW

Sequences:

>Translated_146_residues
MRYLHTMVRVADVDASLDFYCNKLGLKEVRRYENEQGRFTLIFLAASEDEQSGINDKAPLIELTYNWDPEDYKGGRNFGH
LAYEVDDIYATCQHLMDNGVTINRPPRDGNMAFIRSPDGISIELLQKGPAKAKAEPWASMANTGSW
>Mature_146_residues
MRYLHTMVRVADVDASLDFYCNKLGLKEVRRYENEQGRFTLIFLAASEDEQSGINDKAPLIELTYNWDPEDYKGGRNFGH
LAYEVDDIYATCQHLMDNGVTINRPPRDGNMAFIRSPDGISIELLQKGPAKAKAEPWASMANTGSW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=143, Percent_Identity=37.7622377622378, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1787940, Length=135, Percent_Identity=37.037037037037, Blast_Score=96, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6323639, Length=142, Percent_Identity=33.8028169014084, Blast_Score=80, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20129743, Length=144, Percent_Identity=34.0277777777778, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 16549; Mature: 16549

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYLHTMVRVADVDASLDFYCNKLGLKEVRRYENEQGRFTLIFLAASEDEQSGINDKAPL
CCHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCE
IELTYNWDPEDYKGGRNFGHLAYEVDDIYATCQHLMDNGVTINRPPRDGNMAFIRSPDGI
EEEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCC
SIELLQKGPAKAKAEPWASMANTGSW
EEEEECCCCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure
MRYLHTMVRVADVDASLDFYCNKLGLKEVRRYENEQGRFTLIFLAASEDEQSGINDKAPL
CCHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCE
IELTYNWDPEDYKGGRNFGHLAYEVDDIYATCQHLMDNGVTINRPPRDGNMAFIRSPDGI
EEEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCC
SIELLQKGPAKAKAEPWASMANTGSW
EEEEECCCCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]