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The map label for this gene is gcvA [H]

Identifier: 13470425

GI number: 13470425

Start: 88658

End: 89569

Strand: Reverse

Name: gcvA [H]

Synonym: mll0129

Alternate gene names: 13470425

Gene position: 89569-88658 (Counterclockwise)

Preceding gene: 13470426

Following gene: 13470424

Centisome position: 1.27

GC content: 61.95

Gene sequence:

>912_bases
ATGAGTTGGGAGCTACCGCCATTAGGGGCTATCAGAGTCTTTGAAGCCGCCGCGCGGCTGGGCAGCTTTACCAAGGCGGC
TGAAGAACTCGGCATGACCCAGTCGGCCGCCAGCTATCAGATCAAGGTTCTGGAAGAACGCGCAGGAACGCCGCTTTTCA
TAAGAAAAACAAGGCAGATCGCCTTGACCGAAGCCGGACAGCAGCTGGCGCCGCATGCATCCGGCGCGTTCTCGGCCCTG
GCGGATGCCTGGGTCGCCACCAAGGGTGGAGCGACAGGCGTGCTGTCTGTCACGACCATGGAAACCTTCGCAACGAACTG
GCTAGCCGTGTGGCTGGGAACATTCCAGCTGATGCATCCCGATCTTGCCGTGAAGGTCAATACATCGCCCCGGCTGGTCG
ATTTCACACGCGAGGACATGGATATCGGCATCCGGACCGGCACCGGAAATTGGTCCGGCCTGACCGCGCACTATCTGTTC
AAGGCCGACTACACGCCCATGCTCAGTCCAAAACTGGCTGAGAGCGTGGGCGGCATCCGCCGCCCCGAGGATCTCTACAA
GGTGCCGCTATGCTGCGCCGACGATCCCTGGTGGAAAATCTGGTTCGAGGCAGCCGGCGTACCCTTCGAGCCCGATCGCG
TCATTGCGGGCCCGATGCTGGGTTCGCAGGCCTACGACGCAATGGCGGCATTGACCGATCGGGGCGCGGCGATCCTCACC
CGCAACATCTACAGCGCCCTGCTGGCGAACGGTCAGTTGATACAGCCATTTGACGTCCTGGGATCCGACGGTGACGGCTA
TTGGTTGGTGCACCTGGAAAGCCGCCGCAACGTGCCGAAGATCAAGGTGTTCCGCGACTGGGTGCTGGCCCAGACCGCCG
AGATCCGGGCGCAAGAAGGGCGCCAGACTTAG

Upstream 100 bases:

>100_bases
TGGTCCTTTCGGGTTCGGGTTAATGACCAGATCATGCCTGTTCACGGCCTTGGATTCCAATCGAACGTCGCATACTATGC
ATCAGGAGGATTGATGCATC

Downstream 100 bases:

>100_bases
GCCTACAAGGTTCACTCGCAACAGAGGACCAGATGATCGATCACATAACCATCGAAGTGCCCGACCTGGAGAGGAGCAAG
CTCTTCTACGAACGCGCTTT

Product: transcriptional activator AmpR

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSWELPPLGAIRVFEAAARLGSFTKAAEELGMTQSAASYQIKVLEERAGTPLFIRKTRQIALTEAGQQLAPHASGAFSAL
ADAWVATKGGATGVLSVTTMETFATNWLAVWLGTFQLMHPDLAVKVNTSPRLVDFTREDMDIGIRTGTGNWSGLTAHYLF
KADYTPMLSPKLAESVGGIRRPEDLYKVPLCCADDPWWKIWFEAAGVPFEPDRVIAGPMLGSQAYDAMAALTDRGAAILT
RNIYSALLANGQLIQPFDVLGSDGDGYWLVHLESRRNVPKIKVFRDWVLAQTAEIRAQEGRQT

Sequences:

>Translated_303_residues
MSWELPPLGAIRVFEAAARLGSFTKAAEELGMTQSAASYQIKVLEERAGTPLFIRKTRQIALTEAGQQLAPHASGAFSAL
ADAWVATKGGATGVLSVTTMETFATNWLAVWLGTFQLMHPDLAVKVNTSPRLVDFTREDMDIGIRTGTGNWSGLTAHYLF
KADYTPMLSPKLAESVGGIRRPEDLYKVPLCCADDPWWKIWFEAAGVPFEPDRVIAGPMLGSQAYDAMAALTDRGAAILT
RNIYSALLANGQLIQPFDVLGSDGDGYWLVHLESRRNVPKIKVFRDWVLAQTAEIRAQEGRQT
>Mature_302_residues
SWELPPLGAIRVFEAAARLGSFTKAAEELGMTQSAASYQIKVLEERAGTPLFIRKTRQIALTEAGQQLAPHASGAFSALA
DAWVATKGGATGVLSVTTMETFATNWLAVWLGTFQLMHPDLAVKVNTSPRLVDFTREDMDIGIRTGTGNWSGLTAHYLFK
ADYTPMLSPKLAESVGGIRRPEDLYKVPLCCADDPWWKIWFEAAGVPFEPDRVIAGPMLGSQAYDAMAALTDRGAAILTR
NIYSALLANGQLIQPFDVLGSDGDGYWLVHLESRRNVPKIKVFRDWVLAQTAEIRAQEGRQT

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=35.5932203389831, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=30.6896551724138, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=26.2798634812287, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787128, Length=135, Percent_Identity=36.2962962962963, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786401, Length=182, Percent_Identity=28.021978021978, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI145693193, Length=181, Percent_Identity=29.2817679558011, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787589, Length=138, Percent_Identity=34.0579710144928, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081978, Length=188, Percent_Identity=27.6595744680851, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI157672245, Length=140, Percent_Identity=33.5714285714286, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788748, Length=164, Percent_Identity=27.4390243902439, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786713, Length=63, Percent_Identity=50.7936507936508, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33174; Mature: 33043

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWELPPLGAIRVFEAAARLGSFTKAAEELGMTQSAASYQIKVLEERAGTPLFIRKTRQI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEECCEE
ALTEAGQQLAPHASGAFSALADAWVATKGGATGVLSVTTMETFATNWLAVWLGTFQLMHP
EEHHCCHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCC
DLAVKVNTSPRLVDFTREDMDIGIRTGTGNWSGLTAHYLFKADYTPMLSPKLAESVGGIR
CEEEEECCCCCEEEEECCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCHHHHHHHCCCC
RPEDLYKVPLCCADDPWWKIWFEAAGVPFEPDRVIAGPMLGSQAYDAMAALTDRGAAILT
CCHHHHCCCEEECCCCCEEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHCCCHHHHH
RNIYSALLANGQLIQPFDVLGSDGDGYWLVHLESRRNVPKIKVFRDWVLAQTAEIRAQEG
HHHHHHHHCCCCEECHHHHCCCCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHCC
RQT
CCC
>Mature Secondary Structure 
SWELPPLGAIRVFEAAARLGSFTKAAEELGMTQSAASYQIKVLEERAGTPLFIRKTRQI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEECCEE
ALTEAGQQLAPHASGAFSALADAWVATKGGATGVLSVTTMETFATNWLAVWLGTFQLMHP
EEHHCCHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCC
DLAVKVNTSPRLVDFTREDMDIGIRTGTGNWSGLTAHYLFKADYTPMLSPKLAESVGGIR
CEEEEECCCCCEEEEECCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCHHHHHHHCCCC
RPEDLYKVPLCCADDPWWKIWFEAAGVPFEPDRVIAGPMLGSQAYDAMAALTDRGAAILT
CCHHHHCCCEEECCCCCEEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHCCCHHHHH
RNIYSALLANGQLIQPFDVLGSDGDGYWLVHLESRRNVPKIKVFRDWVLAQTAEIRAQEG
HHHHHHHHCCCCEECHHHHCCCCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHCC
RQT
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]