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The map label for this gene is trmJ [H]

Identifier: 13470359

GI number: 13470359

Start: 32191

End: 33102

Strand: Direct

Name: trmJ [H]

Synonym: mlr0038

Alternate gene names: 13470359

Gene position: 32191-33102 (Clockwise)

Preceding gene: 13470355

Following gene: 13470360

Centisome position: 0.46

GC content: 66.01

Gene sequence:

>912_bases
ATGACCGGAAAAGACTTTCAGCGTGGCCCCGGCGGTCCGGCGATCATCCTCGTCGAGCCGCAGCTTGGCGAGAACATCGG
CATGGTCGCGCGCGCCATGGCCAATTTCGGCCTGTCGGAACTGCGTCTGGTCAATCCGCGCGACGGCTGGCCGAGCGAGA
AGGCGCGCGCCGCCGCGAGCCGCGCCGACCATGTCATCGATGCCGTGAAGGTGTTCGACGATCTGGCCTCGGCGCTTGCC
GACCTCAATTTCGTTTTCGCCACCACCGCCAGGCAGCGCGATGGCTTCAAGGCGGTGCGCGGCCCCGTTGAGGCGGGCAG
GGTGCTGCGCGCCCGCCATGGCATGGGGCAGCGCACCGGCATCCTGTTCGGCCGCGAGCGCTTCGGCCTCTACAATGACG
AGGTCGGACTTGCCGACGAGATCGTCACCTTTCCCGTCGATCCTGATTTCTCCTCGCTCAACATCGCTCAGGCCGCGCTG
CTGATGTCCTATGAGTGGATGAAGTCCGGCCTCGAGGACGAGACCAAAACCAATTTTTCAGGGCCGGAGATGAAGCCGGC
CAGCAAGGAAGAATTGCATGGTCTGTTCGCCTATCTCGAAGGCGCGTTGGAAGCGCGCGGCTATTTCCGGCCGGCGCCGA
AGAAGCCGAAAATGGTCGACAATCTGCGCGCCGTGCTGACGCGCGCCGGCTTTGCCGAGCCGGAGCTGAAGGTGCTGCGC
GGCATCATTTCCTCGCTGGACAGGTTCTCGCCGGCAATGCCGCGCGGCGATGGCTCGCCGGGCGATGATCCAAGGCGGCT
TCCGGCCGCCGCGCGCGCGGGGAAGGCAGACGCCGATCGCCAGGTGCGCAAGCCTGCCGGTGATGATGAGGACGCGCCGC
CGCTGGGCGGCAAGGGAACCGACAATGACTGA

Upstream 100 bases:

>100_bases
CGAGACAAGGCTATTTGTCGCCAGGTCACCGACAACTCTTCAAAAAGCCGTATCGATATGGCAGGACGCGGTGACCTCGT
AGCGCAATCATCTGGAACAC

Downstream 100 bases:

>100_bases
CCAGCCGATCCTGATGTTCGATTCCGGCGTCGGCGGTTTGACCGTGCTGCGCGAGGCGCGCGTGCTGATGCCGGACCGGC
GCTTCGTCTATGTCGCGGAC

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTGKDFQRGPGGPAIILVEPQLGENIGMVARAMANFGLSELRLVNPRDGWPSEKARAAASRADHVIDAVKVFDDLASALA
DLNFVFATTARQRDGFKAVRGPVEAGRVLRARHGMGQRTGILFGRERFGLYNDEVGLADEIVTFPVDPDFSSLNIAQAAL
LMSYEWMKSGLEDETKTNFSGPEMKPASKEELHGLFAYLEGALEARGYFRPAPKKPKMVDNLRAVLTRAGFAEPELKVLR
GIISSLDRFSPAMPRGDGSPGDDPRRLPAAARAGKADADRQVRKPAGDDEDAPPLGGKGTDND

Sequences:

>Translated_303_residues
MTGKDFQRGPGGPAIILVEPQLGENIGMVARAMANFGLSELRLVNPRDGWPSEKARAAASRADHVIDAVKVFDDLASALA
DLNFVFATTARQRDGFKAVRGPVEAGRVLRARHGMGQRTGILFGRERFGLYNDEVGLADEIVTFPVDPDFSSLNIAQAAL
LMSYEWMKSGLEDETKTNFSGPEMKPASKEELHGLFAYLEGALEARGYFRPAPKKPKMVDNLRAVLTRAGFAEPELKVLR
GIISSLDRFSPAMPRGDGSPGDDPRRLPAAARAGKADADRQVRKPAGDDEDAPPLGGKGTDND
>Mature_302_residues
TGKDFQRGPGGPAIILVEPQLGENIGMVARAMANFGLSELRLVNPRDGWPSEKARAAASRADHVIDAVKVFDDLASALAD
LNFVFATTARQRDGFKAVRGPVEAGRVLRARHGMGQRTGILFGRERFGLYNDEVGLADEIVTFPVDPDFSSLNIAQAALL
MSYEWMKSGLEDETKTNFSGPEMKPASKEELHGLFAYLEGALEARGYFRPAPKKPKMVDNLRAVLTRAGFAEPELKVLRG
IISSLDRFSPAMPRGDGSPGDDPRRLPAAARAGKADADRQVRKPAGDDEDAPPLGGKGTDND

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=239, Percent_Identity=33.4728033472803, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790865, Length=151, Percent_Identity=39.0728476821192, Blast_Score=103, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 32731; Mature: 32600

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKDFQRGPGGPAIILVEPQLGENIGMVARAMANFGLSELRLVNPRDGWPSEKARAAAS
CCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHEEEECCCCCCCCHHHHHHHH
RADHVIDAVKVFDDLASALADLNFVFATTARQRDGFKAVRGPVEAGRVLRARHGMGQRTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHCCCCCCCC
ILFGRERFGLYNDEVGLADEIVTFPVDPDFSSLNIAQAALLMSYEWMKSGLEDETKTNFS
EEECHHHCCCCCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCC
GPEMKPASKEELHGLFAYLEGALEARGYFRPAPKKPKMVDNLRAVLTRAGFAEPELKVLR
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
GIISSLDRFSPAMPRGDGSPGDDPRRLPAAARAGKADADRQVRKPAGDDEDAPPLGGKGT
HHHHHHHHHCCCCCCCCCCCCCCCHHCCHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCC
DND
CCC
>Mature Secondary Structure 
TGKDFQRGPGGPAIILVEPQLGENIGMVARAMANFGLSELRLVNPRDGWPSEKARAAAS
CCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHEEEECCCCCCCCHHHHHHHH
RADHVIDAVKVFDDLASALADLNFVFATTARQRDGFKAVRGPVEAGRVLRARHGMGQRTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHCCCCCCCC
ILFGRERFGLYNDEVGLADEIVTFPVDPDFSSLNIAQAALLMSYEWMKSGLEDETKTNFS
EEECHHHCCCCCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCC
GPEMKPASKEELHGLFAYLEGALEARGYFRPAPKKPKMVDNLRAVLTRAGFAEPELKVLR
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
GIISSLDRFSPAMPRGDGSPGDDPRRLPAAARAGKADADRQVRKPAGDDEDAPPLGGKGT
HHHHHHHHHCCCCCCCCCCCCCCCHHCCHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCC
DND
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]