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The map label for this gene is tyrS

Identifier: 13470335

GI number: 13470335

Start: 6203

End: 7456

Strand: Reverse

Name: tyrS

Synonym: mll0007

Alternate gene names: 13470335

Gene position: 7456-6203 (Counterclockwise)

Preceding gene: 13470340

Following gene: 13470333

Centisome position: 0.11

GC content: 63.4

Gene sequence:

>1254_bases
ATGCCTGCCTTCAAATCCGATTTCCTGCGCACGATGAGCGAGCGCGGCTTCATCCACCAGATTTCGGATGAGACCGGCCT
CGACCAGCTTTTCGCCAAGGAAACGGTCACCGCCTATGTCGGCTACGACGCCACCGCCACCAGCCTGCATATCGGCAATC
TGATCTCGGCGACCATGCTTTACTGGCTGCAGGAAACCGGGCACCGGCCGATCGCGCTGATGGGCGGCGGCACGTCGATG
ATCGGCGACCCGTCCTTCCGCGACGACCAGCGCAGCCTTTTGACGCCCGAGGCGATCGCCACCAACATCGAAGGCATCAA
GCGCATTTTCGGCCGCATCCTGCGCTTCGGCGACGGCCCGAACGACGCCATCATGGTCAACAATGCCGACTGGCTGATGA
AGCTCAACTATGTCGAGTTCCTGCGCGATGTCGGCCGGCATTTTTCCGTCAACCGCATGCTGACCTTCGACAGCGTCAAG
CTCAGGCTCGACCGCGAGCAGTCGCTGTCGTTCCTCGAATTCAACTACATGATCCTGCAGGGCTACGATTTCGTCGAGCT
CGCCCGGCGCCAGAACTGCCGCCTGCAGATGGGCGGGTCGGACCAGTGGGGCAATATCATCAACGGCGTCGATCTCGGCC
ATCGCATGGGCACGCCGCAGCTTTACGCGCTGACCACGCCGCTGCTCACGACGTCGTCGGGCGCCAAGATGGGCAAGTCG
GCGAAGGGTGCGGTGTGGCTCAACGGCGACCTCTTCTCGCCCTATGATTTCTGGCAGTACTGGCGCAACACCGAGGACGC
CGATGTCGAGCGCTTCCTGAAGATATTCACCCGCCTGCCGCTGCCCGAAATCGCGCGCCTGGCCGCACTCGGCGGCTCCG
AAATCAACGAGGCCAAGAAGATCCTGGCCACGGAGACGACGGCGATCGTCCATGGCCGCGAAGCGGCAAACCAGGCCGAG
GAAACCGCGCGCAAGACCTTCGAGGAAGGGGCGCTCGCCGACACATTGCCGACCGTCGGGGCCGACAAGGCAGCGCTCGA
AGCCGGCATCGGCATCCTGTCGCTGCTGGTCACGGCGGGGCTGGCATCGTCCAATGGCGAGGCACGGCGGCACATACAGG
GCGGCGCGGTTCGCATCAACGACCAGTCGGTCAGCGACGACCGCCGCATGGTCACGCTTCAGGATCTGAGTCCGGAAAAC
GTCGTCAAGCTTTCACTCGGCAAGAAAAAGCACGTTCTGGTGCGGCCGGCCTGA

Upstream 100 bases:

>100_bases
GCAAAACCGGATTCCACTTTTGCTGATCGCGCTTGGATGCTAAACGCGCGCCTTCATATAGGCCGCATCAGACCGGCTGG
ACGCTTTCAGGAAACAAAAT

Downstream 100 bases:

>100_bases
GGCCGTTCATCGATATTCGAAGATCGACCTGAAGATGCCCGGTGCGATCACCGACAGCGGATTGATATCCAGCACCGGCT
TGTTGGCATTGCCCCTGAGC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MPAFKSDFLRTMSERGFIHQISDETGLDQLFAKETVTAYVGYDATATSLHIGNLISATMLYWLQETGHRPIALMGGGTSM
IGDPSFRDDQRSLLTPEAIATNIEGIKRIFGRILRFGDGPNDAIMVNNADWLMKLNYVEFLRDVGRHFSVNRMLTFDSVK
LRLDREQSLSFLEFNYMILQGYDFVELARRQNCRLQMGGSDQWGNIINGVDLGHRMGTPQLYALTTPLLTTSSGAKMGKS
AKGAVWLNGDLFSPYDFWQYWRNTEDADVERFLKIFTRLPLPEIARLAALGGSEINEAKKILATETTAIVHGREAANQAE
ETARKTFEEGALADTLPTVGADKAALEAGIGILSLLVTAGLASSNGEARRHIQGGAVRINDQSVSDDRRMVTLQDLSPEN
VVKLSLGKKKHVLVRPA

Sequences:

>Translated_417_residues
MPAFKSDFLRTMSERGFIHQISDETGLDQLFAKETVTAYVGYDATATSLHIGNLISATMLYWLQETGHRPIALMGGGTSM
IGDPSFRDDQRSLLTPEAIATNIEGIKRIFGRILRFGDGPNDAIMVNNADWLMKLNYVEFLRDVGRHFSVNRMLTFDSVK
LRLDREQSLSFLEFNYMILQGYDFVELARRQNCRLQMGGSDQWGNIINGVDLGHRMGTPQLYALTTPLLTTSSGAKMGKS
AKGAVWLNGDLFSPYDFWQYWRNTEDADVERFLKIFTRLPLPEIARLAALGGSEINEAKKILATETTAIVHGREAANQAE
ETARKTFEEGALADTLPTVGADKAALEAGIGILSLLVTAGLASSNGEARRHIQGGAVRINDQSVSDDRRMVTLQDLSPEN
VVKLSLGKKKHVLVRPA
>Mature_416_residues
PAFKSDFLRTMSERGFIHQISDETGLDQLFAKETVTAYVGYDATATSLHIGNLISATMLYWLQETGHRPIALMGGGTSMI
GDPSFRDDQRSLLTPEAIATNIEGIKRIFGRILRFGDGPNDAIMVNNADWLMKLNYVEFLRDVGRHFSVNRMLTFDSVKL
RLDREQSLSFLEFNYMILQGYDFVELARRQNCRLQMGGSDQWGNIINGVDLGHRMGTPQLYALTTPLLTTSSGAKMGKSA
KGAVWLNGDLFSPYDFWQYWRNTEDADVERFLKIFTRLPLPEIARLAALGGSEINEAKKILATETTAIVHGREAANQAEE
TARKTFEEGALADTLPTVGADKAALEAGIGILSLLVTAGLASSNGEARRHIQGGAVRINDQSVSDDRRMVTLQDLSPENV
VKLSLGKKKHVLVRPA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=436, Percent_Identity=33.4862385321101, Blast_Score=230, Evalue=2e-60,
Organism=Escherichia coli, GI1787925, Length=419, Percent_Identity=39.8568019093079, Blast_Score=316, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI25145802, Length=405, Percent_Identity=33.0864197530864, Blast_Score=178, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI25145805, Length=330, Percent_Identity=30.9090909090909, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6325160, Length=441, Percent_Identity=31.2925170068027, Blast_Score=214, Evalue=3e-56,
Organism=Drosophila melanogaster, GI20130383, Length=437, Percent_Identity=36.8421052631579, Blast_Score=264, Evalue=7e-71,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_RHILO (Q98NS5)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_101900.1
- ProteinModelPortal:   Q98NS5
- SMR:   Q98NS5
- GeneID:   1224563
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll0007
- NMPDR:   fig|266835.1.peg.5
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- ProtClustDB:   PRK05912
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 46133; Mature: 46002

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 39-39 BINDING 176-176 BINDING 180-180 BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAFKSDFLRTMSERGFIHQISDETGLDQLFAKETVTAYVGYDATATSLHIGNLISATML
CCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHH
YWLQETGHRPIALMGGGTSMIGDPSFRDDQRSLLTPEAIATNIEGIKRIFGRILRFGDGP
HHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
NDAIMVNNADWLMKLNYVEFLRDVGRHFSVNRMLTFDSVKLRLDREQSLSFLEFNYMILQ
CCEEEEECCHHEEEEHHHHHHHHHHHCCCCCEEEEECCEEEEECHHHCCCEEEEEEEEEE
GYDFVELARRQNCRLQMGGSDQWGNIINGVDLGHRMGTPQLYALTTPLLTTSSGAKMGKS
CCCHHHHHHCCCCEEEECCCCCCCCHHCCCCCHHHCCCCEEEEEECCCEECCCCCCCCCC
AKGAVWLNGDLFSPYDFWQYWRNTEDADVERFLKIFTRLPLPEIARLAALGGSEINEAKK
CCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHH
ILATETTAIVHGREAANQAEETARKTFEEGALADTLPTVGADKAALEAGIGILSLLVTAG
HHHHHHHHEEECHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
LASSNGEARRHIQGGAVRINDQSVSDDRRMVTLQDLSPENVVKLSLGKKKHVLVRPA
CCCCCCHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCCEEEEECCCCCEEEEECC
>Mature Secondary Structure 
PAFKSDFLRTMSERGFIHQISDETGLDQLFAKETVTAYVGYDATATSLHIGNLISATML
CCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHH
YWLQETGHRPIALMGGGTSMIGDPSFRDDQRSLLTPEAIATNIEGIKRIFGRILRFGDGP
HHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
NDAIMVNNADWLMKLNYVEFLRDVGRHFSVNRMLTFDSVKLRLDREQSLSFLEFNYMILQ
CCEEEEECCHHEEEEHHHHHHHHHHHCCCCCEEEEECCEEEEECHHHCCCEEEEEEEEEE
GYDFVELARRQNCRLQMGGSDQWGNIINGVDLGHRMGTPQLYALTTPLLTTSSGAKMGKS
CCCHHHHHHCCCCEEEECCCCCCCCHHCCCCCHHHCCCCEEEEEECCCEECCCCCCCCCC
AKGAVWLNGDLFSPYDFWQYWRNTEDADVERFLKIFTRLPLPEIARLAALGGSEINEAKK
CCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHH
ILATETTAIVHGREAANQAEETARKTFEEGALADTLPTVGADKAALEAGIGILSLLVTAG
HHHHHHHHEEECHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
LASSNGEARRHIQGGAVRINDQSVSDDRRMVTLQDLSPENVVKLSLGKKKHVLVRPA
CCCCCCHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCCEEEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968