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The map label for this gene is ulaA [H]

Identifier: 161723313

GI number: 161723313

Start: 900779

End: 902569

Strand: Direct

Name: ulaA [H]

Synonym: PM0764

Alternate gene names: 161723313

Gene position: 900779-902569 (Clockwise)

Preceding gene: 15602623

Following gene: 15602630

Centisome position: 39.9

GC content: 39.98

Gene sequence:

>1791_bases
ATGGAAACACTCTATAACCTATTCTTAAGCTTTAATAATCAAGTCCTTTCTAAAGCCCCCTTTCTACTTGGTATTGTTGC
TTGTATTGGGTACATTCTATTGAAAAAAGACACAACAACAGTTATCAAAGGAACAATAAAAACAATTGTTGGTTTTATGA
TTGTGCAGGCGGGATCTGGCTTTCTTGTCGCAAACTTCAAGCCAATAATTGAGGGGTTATCAAAATATCATAATTTGACG
GGGGCAGTGATTGACCCTTACACCAGTATGCAAGCCACCATCCAAACAATGGCAGATAACTATGCGTGGGTTGGCTACGC
GGTTATTCTGGCATTGTTTTTAAATATCTTATTAGTCGTATGCCGTAGAATTACAGGGATTCGAACTATCATGCTGACGG
GACATATTATGTTCCAACAAGCAGGTTTAGTCGCTGTATTCTATATGATTATTGGTGCCTCAATGTGGGAAACGGTCATT
TATACTGCCGTATTAATGGCGCTATATTGGGGAATCTCATCAAATATCATGTATAAACCCACCCAAGCAGTTACTGGTGG
TGCAGGTTTTTCTATTGGACATCAACAACAAATCGCTTCTTGGATCGCAGTCAAACTCGCGCCAAAACTTGGCGACAAAA
ATGATACCGTAGATAAAATGAAATTACCGAAATGGCTACATATTTTTCATGACAGTATTTCCGCAACTGCACTTGTTATG
ACTGTATTTTTCGGCATCATTTTACTCTCTTTTGGCTTAGATAACTTGCAACAAATGGCAGGCAAAACCCACTGGTTTAT
GTACATTTTTGAAATGGGCTTAAAATTTGCTGTTGCTATCCAAATCATCGTCACTGGTGTACGGATGTTTGTTGCTGAGC
TTTCAGAAGCATTCAAAGGAATATCTGAGCGCGTTATTCCAAACTCTGTACTCGCTATTGACTGTGCGGCCATTTATGCT
TTTTCGCCAAATGCAATGGTCTTTGGCTTTATGTGGGGGGCGATTGGACAATTTGTAGCTGTCGGTCTGCTCCTCGGTTT
TAGTTCACCTATTCTGATTATTCCAGGCTTCATTCCGATGTTTTTCTCAAATGCAACGATCGGTGTCTTTGCGAACCAAT
TTGGTGGCTGGAAATCCGTCATGAAAATTTGTTTCATTATGGGAATCATAGAAGTACTTGGTTCAGCTTGGGTTATTCAC
TTACTTGCCACCCAAGGTACAACGTTTAATGGTTGGATGGGAATGGCGGACTGGGCATTATTTTTCCCACCAATATTGCA
AGGCATTGTTAGCATACCGGGTTTCTTTTTTGTTTTACTGACATTAGCCATTGTTTATATGGTGTTTGCGTCAAAGCAAT
TACGTTCAGAAGAGGCCGCTGCCGCTGCACTTGGTAAAACCTTGGAACAAATGGATGGTTATGACAAGACAGAACATAGC
CCAATCACACCGGATGACAGCGTCCAACATGATGTTCTAAAAACAGCCGTTAACCCTATTCGAATCCTGGCAGTTTGTGG
TAGCGGGCAGGGTTCATCAATGATGATGAAAATGAAGATTAAAAGTTATCTGGACAAGCATAACATACCAAATACCTTGG
ATTCCTGTGCAGTGACCGATTATAAAGGCAAATTAAATGAAATCGATATTATCGTTGCTTCTCGTCATCTTGCCGATGAA
ATTGAAGTGGGTGAAGATAAATTTGTCTTGGGTGTACAAAACATGCTAAATCCAAATTCCTTTGGTCCAGAATTAATTGC
CTTAATCAAAAAATATCAGCAAACCACTTAA

Upstream 100 bases:

>100_bases
TTTTTTTGCTGTTATGCTTATACCTAGATTTCTTGGATCAGGCCTTATCCAGAAATCTTTCATGCAAATATACACAATGA
AAAAATCCAGAGGTGTCACT

Downstream 100 bases:

>100_bases
AGGAGACGAATCCTTATTCAATCATCAACTAAATAGGACATACCATGAATTTAAAACAGTCACTCATAGAAAATAATTCA
ATCAAACTTCATCAAACTGC

Product: PTS system ascorbate-specific transporter subunits IICB

Products: protein histidine; sugar phosphate

Alternate protein names: Ascorbate-specific PTS system EIIC component [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
METLYNLFLSFNNQVLSKAPFLLGIVACIGYILLKKDTTTVIKGTIKTIVGFMIVQAGSGFLVANFKPIIEGLSKYHNLT
GAVIDPYTSMQATIQTMADNYAWVGYAVILALFLNILLVVCRRITGIRTIMLTGHIMFQQAGLVAVFYMIIGASMWETVI
YTAVLMALYWGISSNIMYKPTQAVTGGAGFSIGHQQQIASWIAVKLAPKLGDKNDTVDKMKLPKWLHIFHDSISATALVM
TVFFGIILLSFGLDNLQQMAGKTHWFMYIFEMGLKFAVAIQIIVTGVRMFVAELSEAFKGISERVIPNSVLAIDCAAIYA
FSPNAMVFGFMWGAIGQFVAVGLLLGFSSPILIIPGFIPMFFSNATIGVFANQFGGWKSVMKICFIMGIIEVLGSAWVIH
LLATQGTTFNGWMGMADWALFFPPILQGIVSIPGFFFVLLTLAIVYMVFASKQLRSEEAAAAALGKTLEQMDGYDKTEHS
PITPDDSVQHDVLKTAVNPIRILAVCGSGQGSSMMMKMKIKSYLDKHNIPNTLDSCAVTDYKGKLNEIDIIVASRHLADE
IEVGEDKFVLGVQNMLNPNSFGPELIALIKKYQQTT

Sequences:

>Translated_596_residues
METLYNLFLSFNNQVLSKAPFLLGIVACIGYILLKKDTTTVIKGTIKTIVGFMIVQAGSGFLVANFKPIIEGLSKYHNLT
GAVIDPYTSMQATIQTMADNYAWVGYAVILALFLNILLVVCRRITGIRTIMLTGHIMFQQAGLVAVFYMIIGASMWETVI
YTAVLMALYWGISSNIMYKPTQAVTGGAGFSIGHQQQIASWIAVKLAPKLGDKNDTVDKMKLPKWLHIFHDSISATALVM
TVFFGIILLSFGLDNLQQMAGKTHWFMYIFEMGLKFAVAIQIIVTGVRMFVAELSEAFKGISERVIPNSVLAIDCAAIYA
FSPNAMVFGFMWGAIGQFVAVGLLLGFSSPILIIPGFIPMFFSNATIGVFANQFGGWKSVMKICFIMGIIEVLGSAWVIH
LLATQGTTFNGWMGMADWALFFPPILQGIVSIPGFFFVLLTLAIVYMVFASKQLRSEEAAAAALGKTLEQMDGYDKTEHS
PITPDDSVQHDVLKTAVNPIRILAVCGSGQGSSMMMKMKIKSYLDKHNIPNTLDSCAVTDYKGKLNEIDIIVASRHLADE
IEVGEDKFVLGVQNMLNPNSFGPELIALIKKYQQTT
>Mature_596_residues
METLYNLFLSFNNQVLSKAPFLLGIVACIGYILLKKDTTTVIKGTIKTIVGFMIVQAGSGFLVANFKPIIEGLSKYHNLT
GAVIDPYTSMQATIQTMADNYAWVGYAVILALFLNILLVVCRRITGIRTIMLTGHIMFQQAGLVAVFYMIIGASMWETVI
YTAVLMALYWGISSNIMYKPTQAVTGGAGFSIGHQQQIASWIAVKLAPKLGDKNDTVDKMKLPKWLHIFHDSISATALVM
TVFFGIILLSFGLDNLQQMAGKTHWFMYIFEMGLKFAVAIQIIVTGVRMFVAELSEAFKGISERVIPNSVLAIDCAAIYA
FSPNAMVFGFMWGAIGQFVAVGLLLGFSSPILIIPGFIPMFFSNATIGVFANQFGGWKSVMKICFIMGIIEVLGSAWVIH
LLATQGTTFNGWMGMADWALFFPPILQGIVSIPGFFFVLLTLAIVYMVFASKQLRSEEAAAAALGKTLEQMDGYDKTEHS
PITPDDSVQHDVLKTAVNPIRILAVCGSGQGSSMMMKMKIKSYLDKHNIPNTLDSCAVTDYKGKLNEIDIIVASRHLADE
IEVGEDKFVLGVQNMLNPNSFGPELIALIKKYQQTT

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active- transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3037

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ulaA family [H]

Homologues:

Organism=Escherichia coli, GI87082388, Length=467, Percent_Identity=62.7408993576017, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI1790638, Length=93, Percent_Identity=55.9139784946236, Blast_Score=115, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004703 [H]

Pfam domain/function: PF03611 EIIC-GAT [H]

EC number: 2.7.1.69

Molecular weight: Translated: 65393; Mature: 65393

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLYNLFLSFNNQVLSKAPFLLGIVACIGYILLKKDTTTVIKGTIKTIVGFMIVQAGSG
CHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
FLVANFKPIIEGLSKYHNLTGAVIDPYTSMQATIQTMADNYAWVGYAVILALFLNILLVV
EEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
CRRITGIRTIMLTGHIMFQQAGLVAVFYMIIGASMWETVIYTAVLMALYWGISSNIMYKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
TQAVTGGAGFSIGHQQQIASWIAVKLAPKLGDKNDTVDKMKLPKWLHIFHDSISATALVM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHH
TVFFGIILLSFGLDNLQQMAGKTHWFMYIFEMGLKFAVAIQIIVTGVRMFVAELSEAFKG
HHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISERVIPNSVLAIDCAAIYAFSPNAMVFGFMWGAIGQFVAVGLLLGFSSPILIIPGFIPM
HHHHCCCCCHHHEEHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHH
FFSNATIGVFANQFGGWKSVMKICFIMGIIEVLGSAWVIHLLATQGTTFNGWMGMADWAL
HHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
FFPPILQGIVSIPGFFFVLLTLAIVYMVFASKQLRSEEAAAAALGKTLEQMDGYDKTEHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PITPDDSVQHDVLKTAVNPIRILAVCGSGQGSSMMMKMKIKSYLDKHNIPNTLDSCAVTD
CCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHCEEEC
YKGKLNEIDIIVASRHLADEIEVGEDKFVLGVQNMLNPNSFGPELIALIKKYQQTT
CCCCCCEEEEEEECHHHHHHHCCCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
METLYNLFLSFNNQVLSKAPFLLGIVACIGYILLKKDTTTVIKGTIKTIVGFMIVQAGSG
CHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
FLVANFKPIIEGLSKYHNLTGAVIDPYTSMQATIQTMADNYAWVGYAVILALFLNILLVV
EEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
CRRITGIRTIMLTGHIMFQQAGLVAVFYMIIGASMWETVIYTAVLMALYWGISSNIMYKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
TQAVTGGAGFSIGHQQQIASWIAVKLAPKLGDKNDTVDKMKLPKWLHIFHDSISATALVM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHH
TVFFGIILLSFGLDNLQQMAGKTHWFMYIFEMGLKFAVAIQIIVTGVRMFVAELSEAFKG
HHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISERVIPNSVLAIDCAAIYAFSPNAMVFGFMWGAIGQFVAVGLLLGFSSPILIIPGFIPM
HHHHCCCCCHHHEEHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHH
FFSNATIGVFANQFGGWKSVMKICFIMGIIEVLGSAWVIHLLATQGTTFNGWMGMADWAL
HHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
FFPPILQGIVSIPGFFFVLLTLAIVYMVFASKQLRSEEAAAAALGKTLEQMDGYDKTEHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PITPDDSVQHDVLKTAVNPIRILAVCGSGQGSSMMMKMKIKSYLDKHNIPNTLDSCAVTD
CCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHCEEEC
YKGKLNEIDIIVASRHLADEIEVGEDKFVLGVQNMLNPNSFGPELIALIKKYQQTT
CCCCCCEEEEEEECHHHHHHHCCCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]