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The map label for this gene is dacA [H]

Identifier: 15603792

GI number: 15603792

Start: 2166653

End: 2167837

Strand: Direct

Name: dacA [H]

Synonym: PM1927

Alternate gene names: 15603792

Gene position: 2166653-2167837 (Clockwise)

Preceding gene: 15603791

Following gene: 15603793

Centisome position: 95.98

GC content: 38.82

Gene sequence:

>1185_bases
ATGTTTAAAACAGTTGTAACGAAAAGTAAAATCGCATTGATAACGGGCTTATGTATTGCCCCAAGTTTGTTGGCGCAAGA
TATTGATTATGGGATTGCGGTGCCACAGTTGAATGCGCAAACCTTTATTTTAATGGATTATAATTCGGGTGCGGTTATTG
CTGCATTGAATCCTGATCAACGCCAATATCCTGCCTCATTAACAAAAATGATGACCAGTTATGTCGTTGGTGATGCTTTA
AAACAAGGTAAAATAAATAATGCGGATAGAGTGACGATTGGCGAAAGTGCATGGGGAAAGAATTTTCCTGGCTCGTCAAA
AATGTTCTTAAATTTAAATCAGCAAGTGTCTGTGTCTGATTTAAACCGCGGTGTGATTATTGTGTCAGGTAATGATGCCT
CTGTGGCCATTGCAGAGCATGTTGCTGGTTCTGTACCGGCCTTTGTTGACGCTATGAATAAATATGCGCAACAATTTGGC
TTAAAAAATACGCATTTTACCACGGTACATGGTTTAGATGAGGATAATCAATATTCCTCTGCCCGCGATATGGCGATTAT
TGGTGCGCATTTAATCCGTGATTTACCGGAAGAATATAAAATTTACGCGGAAAAGGATTTTACTTTTAATAAGATTAAAC
AGAATAACCGTAATGGCTTGTTATGGGATAAAAGCATTCAAGTGGATGGCATTAAAACGGGGCATACGGATAAAGCAGGT
TATAACTTAGTTGCTTCAGCGACCAACGCAAACAATATGCGTTTGATTTCGGTGGTGATGGGTGTACCAACGATTAAAGG
ACGTGAAGTCGAAAGTAAAAAATTATTACAGTGGGGATTCGCGAATTTTGATACTTTTAAAACCCTTGAAGCGGGTAAAG
CGATTTCTGAGCAATCTGTTTATTATGGTGACATGGGGAAAGTGTCTTTAGGGGTATTACAAGATGGATTTATTACGATC
CCGAAAGGCAAACAAACTGAGTTAAAAGCCCGTTATGAACTCAATAAAAAATACTTGCAAGCACCGTTAGCGAAAGGTGA
AGTGGTGGGTCAAGTCGTTTATCAATTAGAGGGTAAAGACATCGCGAAAATTGATTTACAAGTGATGCAAGAAGTCCAAG
AAGGCGGTATTTTTGGTAAGGCTTGGGATTGGTTAGTCCTAACTGTAAAAAGTTTGTTTGATTAA

Upstream 100 bases:

>100_bases
TCAATGACGTCAAAGCACAATTAAGAAAATTGAATTACTCGAAACCATTAATTGTGTACACCTTTGACGATTAACCTTAT
TTAAGATGGATACGGAGAGT

Downstream 100 bases:

>100_bases
TCAAAAACTCCCTCCATATTGAAAAGCAAGAAACCGCAATTTGATGACCGCACTTTTTAGTGAAAAGTGCGGTTCATCTT
TGATAAAGAGTAGGATGTAA

Product: DacA

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MFKTVVTKSKIALITGLCIAPSLLAQDIDYGIAVPQLNAQTFILMDYNSGAVIAALNPDQRQYPASLTKMMTSYVVGDAL
KQGKINNADRVTIGESAWGKNFPGSSKMFLNLNQQVSVSDLNRGVIIVSGNDASVAIAEHVAGSVPAFVDAMNKYAQQFG
LKNTHFTTVHGLDEDNQYSSARDMAIIGAHLIRDLPEEYKIYAEKDFTFNKIKQNNRNGLLWDKSIQVDGIKTGHTDKAG
YNLVASATNANNMRLISVVMGVPTIKGREVESKKLLQWGFANFDTFKTLEAGKAISEQSVYYGDMGKVSLGVLQDGFITI
PKGKQTELKARYELNKKYLQAPLAKGEVVGQVVYQLEGKDIAKIDLQVMQEVQEGGIFGKAWDWLVLTVKSLFD

Sequences:

>Translated_394_residues
MFKTVVTKSKIALITGLCIAPSLLAQDIDYGIAVPQLNAQTFILMDYNSGAVIAALNPDQRQYPASLTKMMTSYVVGDAL
KQGKINNADRVTIGESAWGKNFPGSSKMFLNLNQQVSVSDLNRGVIIVSGNDASVAIAEHVAGSVPAFVDAMNKYAQQFG
LKNTHFTTVHGLDEDNQYSSARDMAIIGAHLIRDLPEEYKIYAEKDFTFNKIKQNNRNGLLWDKSIQVDGIKTGHTDKAG
YNLVASATNANNMRLISVVMGVPTIKGREVESKKLLQWGFANFDTFKTLEAGKAISEQSVYYGDMGKVSLGVLQDGFITI
PKGKQTELKARYELNKKYLQAPLAKGEVVGQVVYQLEGKDIAKIDLQVMQEVQEGGIFGKAWDWLVLTVKSLFD
>Mature_394_residues
MFKTVVTKSKIALITGLCIAPSLLAQDIDYGIAVPQLNAQTFILMDYNSGAVIAALNPDQRQYPASLTKMMTSYVVGDAL
KQGKINNADRVTIGESAWGKNFPGSSKMFLNLNQQVSVSDLNRGVIIVSGNDASVAIAEHVAGSVPAFVDAMNKYAQQFG
LKNTHFTTVHGLDEDNQYSSARDMAIIGAHLIRDLPEEYKIYAEKDFTFNKIKQNNRNGLLWDKSIQVDGIKTGHTDKAG
YNLVASATNANNMRLISVVMGVPTIKGREVESKKLLQWGFANFDTFKTLEAGKAISEQSVYYGDMGKVSLGVLQDGFITI
PKGKQTELKARYELNKKYLQAPLAKGEVVGQVVYQLEGKDIAKIDLQVMQEVQEGGIFGKAWDWLVLTVKSLFD

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=392, Percent_Identity=56.6326530612245, Blast_Score=423, Evalue=1e-119,
Organism=Escherichia coli, GI1787062, Length=380, Percent_Identity=51.5789473684211, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI87082022, Length=381, Percent_Identity=44.3569553805774, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI87082054, Length=234, Percent_Identity=29.4871794871795, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43249; Mature: 43249

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKTVVTKSKIALITGLCIAPSLLAQDIDYGIAVPQLNAQTFILMDYNSGAVIAALNPDQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEEECCCEEEEEECCCC
RQYPASLTKMMTSYVVGDALKQGKINNADRVTIGESAWGKNFPGSSKMFLNLNQQVSVSD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCEEEHEE
LNRGVIIVSGNDASVAIAEHVAGSVPAFVDAMNKYAQQFGLKNTHFTTVHGLDEDNQYSS
CCCCEEEEECCCCEEEEHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
ARDMAIIGAHLIRDLPEEYKIYAEKDFTFNKIKQNNRNGLLWDKSIQVDGIKTGHTDKAG
HHHHHHHHHHHHHHCCHHHEEEEECCCCHHHHHCCCCCCEEEECCEEECCCCCCCCCCCC
YNLVASATNANNMRLISVVMGVPTIKGREVESKKLLQWGFANFDTFKTLEAGKAISEQSV
CEEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCCCCCCE
YYGDMGKVSLGVLQDGFITIPKGKQTELKARYELNKKYLQAPLAKGEVVGQVVYQLEGKD
EECCCCCEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHEEECCCC
IAKIDLQVMQEVQEGGIFGKAWDWLVLTVKSLFD
EEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MFKTVVTKSKIALITGLCIAPSLLAQDIDYGIAVPQLNAQTFILMDYNSGAVIAALNPDQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEEECCCEEEEEECCCC
RQYPASLTKMMTSYVVGDALKQGKINNADRVTIGESAWGKNFPGSSKMFLNLNQQVSVSD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCEEEHEE
LNRGVIIVSGNDASVAIAEHVAGSVPAFVDAMNKYAQQFGLKNTHFTTVHGLDEDNQYSS
CCCCEEEEECCCCEEEEHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
ARDMAIIGAHLIRDLPEEYKIYAEKDFTFNKIKQNNRNGLLWDKSIQVDGIKTGHTDKAG
HHHHHHHHHHHHHHCCHHHEEEEECCCCHHHHHCCCCCCEEEECCEEECCCCCCCCCCCC
YNLVASATNANNMRLISVVMGVPTIKGREVESKKLLQWGFANFDTFKTLEAGKAISEQSV
CEEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCCCCCCE
YYGDMGKVSLGVLQDGFITIPKGKQTELKARYELNKKYLQAPLAKGEVVGQVVYQLEGKD
EECCCCCEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHEEECCCC
IAKIDLQVMQEVQEGGIFGKAWDWLVLTVKSLFD
EEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]