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The map label for this gene is folA [H]

Identifier: 15603758

GI number: 15603758

Start: 2134822

End: 2135316

Strand: Reverse

Name: folA [H]

Synonym: PM1893

Alternate gene names: 15603758

Gene position: 2135316-2134822 (Counterclockwise)

Preceding gene: 15603759

Following gene: 15603754

Centisome position: 94.59

GC content: 41.41

Gene sequence:

>495_bases
ATGACCTTTAGCCTTATCGTCGCTATGACGAAAAACCATGTCATTGGTAAAGACAATCAAATGCCTTGGCATCTGCCTGC
CGATTTAGCGTGGTTTCGCCAAAATACCACGGGTAAACCGGTGATTATGGGACGAAAAACCTTTGAAAGCATTGGGCGTC
CATTACCAAAACGCACTAATTTAGTGCTTTCTCGTCAGCCATACCTACATGAGGGCGTGATTTGGCAAAACAGCCTTGAA
AGTGCGGTGGATTTTGTCAAAGATTCACCTGAAATCCTGTTGATTGGCGGGGGCGAATTATTTAAGCAATATTTACATAA
AGCCGACAAGCTCTATCTCACTGAAATCCAAACAGAATTAGAAGGCGATACCTTCTTTCCTGAAATTGTATGGCAAGACT
GGCAAATTGAATATGAAGCATGGCACCAAGCCGATGAAAAAAATCCTTATGACTGTCGCTTTTTAATTTTATCGCGTAAC
ACTAAAAATGAATGA

Upstream 100 bases:

>100_bases
TGCATTAGTTTTCGGACTCGCATTGCCGGGCTTACTGTTCTATCGGCAAAAACCTGTGGTATAGTAAACACACCTTTAGT
TTGCCTGTCAGGAACTCACT

Downstream 100 bases:

>100_bases
CATAACACGTTCAATAAGAACGATGTAAAGGGCGCAAGCATTAAGCAAGCGCCCTTTTCTTCATTTATCCAGCGGATCCT
CACAAAAAAAATCTCAGCAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MTFSLIVAMTKNHVIGKDNQMPWHLPADLAWFRQNTTGKPVIMGRKTFESIGRPLPKRTNLVLSRQPYLHEGVIWQNSLE
SAVDFVKDSPEILLIGGGELFKQYLHKADKLYLTEIQTELEGDTFFPEIVWQDWQIEYEAWHQADEKNPYDCRFLILSRN
TKNE

Sequences:

>Translated_164_residues
MTFSLIVAMTKNHVIGKDNQMPWHLPADLAWFRQNTTGKPVIMGRKTFESIGRPLPKRTNLVLSRQPYLHEGVIWQNSLE
SAVDFVKDSPEILLIGGGELFKQYLHKADKLYLTEIQTELEGDTFFPEIVWQDWQIEYEAWHQADEKNPYDCRFLILSRN
TKNE
>Mature_163_residues
TFSLIVAMTKNHVIGKDNQMPWHLPADLAWFRQNTTGKPVIMGRKTFESIGRPLPKRTNLVLSRQPYLHEGVIWQNSLES
AVDFVKDSPEILLIGGGELFKQYLHKADKLYLTEIQTELEGDTFFPEIVWQDWQIEYEAWHQADEKNPYDCRFLILSRNT
KNE

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=159, Percent_Identity=34.5911949685535, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1786233, Length=157, Percent_Identity=52.2292993630573, Blast_Score=173, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17505899, Length=147, Percent_Identity=34.0136054421769, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324810, Length=134, Percent_Identity=32.089552238806, Blast_Score=68, Evalue=8e-13,
Organism=Drosophila melanogaster, GI116008026, Length=147, Percent_Identity=34.0136054421769, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24647458, Length=147, Percent_Identity=34.0136054421769, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19193; Mature: 19062

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSLIVAMTKNHVIGKDNQMPWHLPADLAWFRQNTTGKPVIMGRKTFESIGRPLPKRTN
CCEEEEEEEECCCCCCCCCCCCEECCCHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCCC
LVLSRQPYLHEGVIWQNSLESAVDFVKDSPEILLIGGGELFKQYLHKADKLYLTEIQTEL
EEEECCCCCCCCEEEHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCEEEEHHHHHC
EGDTFFPEIVWQDWQIEYEAWHQADEKNPYDCRFLILSRNTKNE
CCCCCCHHHCCCCCEEEHHHHCCCCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure 
TFSLIVAMTKNHVIGKDNQMPWHLPADLAWFRQNTTGKPVIMGRKTFESIGRPLPKRTN
CEEEEEEEECCCCCCCCCCCCEECCCHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCCC
LVLSRQPYLHEGVIWQNSLESAVDFVKDSPEILLIGGGELFKQYLHKADKLYLTEIQTEL
EEEECCCCCCCCEEEHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCEEEEHHHHHC
EGDTFFPEIVWQDWQIEYEAWHQADEKNPYDCRFLILSRNTKNE
CCCCCCHHHCCCCCEEEHHHHCCCCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]