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The map label for this gene is eno [H]

Identifier: 15603736

GI number: 15603736

Start: 2112181

End: 2113482

Strand: Reverse

Name: eno [H]

Synonym: PM1871

Alternate gene names: 15603736

Gene position: 2113482-2112181 (Counterclockwise)

Preceding gene: 15603737

Following gene: 15603732

Centisome position: 93.62

GC content: 42.32

Gene sequence:

>1302_bases
ATGGCAAAAATCGTTAAAGTGATCGGCCGTGAAATCATCGATTCTCGTGGTAACCCAACTGTTGAAGCAGAAGTCCATTT
AGAAGGTGGCTTTGTGGGTCTAGCCGCCGCACCATCAGGCGCATCAACTGGTTCACGCGAAGCATTAGAATTACGTGACG
GTGACAAAGCACGTTTCTTAGGTAAAGGTGTGTTAAAAGCTGTTGCTGCAGTGAACAACGAAATTGCACAAGCTCTCGTT
GGTAAAGATGCGACAAACCAAGCTGAAATCGACCAAATTATGATCGATTTAGATGGCACAGAAAACAAATCTAAATTCGG
TGCAAACGCCATTTTAGCGGTTTCTTTAGCTAACGCAAAAGCAGCGGCGGCGGCAAAAGGTATGCCATTATTTGCTTGGA
TTGCAGAACTTAACGGTACGCCAGGTCAATACTCTATGCCATTACCAATGATGAACATCATCAACGGTGGTGAGCACGCA
GACAACAACGTTGATATCCAAGAATTTATGATTCAACCAGTGGGTGCGAAAACCTTAAAAGAAGCCCTTCGTATCGGTGC
GGAAGTATTCCACAACCTTGCGAAAGTATTAAAAGGCAAAGGCTTAAGCACAGCAGTCGGTGACGAAGGTGGTTTTGCAC
CTAACTTAGAATCTAACGCAGCAGCGCTTGCTTGTATCAAAGAAGCCGTAGAAAAAGCGGGTTATGTGTTAGGTAAAGAT
GTCACATTAGCAATGGACTGTGCATCATCTGAGTTCTATAACAAAGAAAATGGTATGTACGAAATGAAAGGTGAAGGTAA
ATCATTCACTTCTCAAGAATTCACACACTACTTAGAAGAATTATGTAAAGAATACCCAATCGTGTCTATCGAAGATGGTC
AAGATGAGTCAGACTGGGAAGGTTTCGCCTACCAAACTAAAGTATTAGGCGACAAAGTTCAATTAGTTGGTGATGACTTA
TTCGTAACGAATACCAAAATCTTAAAAGAAGGTATCGAAAAAGGTATCGCGAATTCGATCTTAATCAAATTCAACCAAAT
CGGTTCATTAACTGAAACGTTAGCGGCAATCAAAATGGCGAAAGACGCAGGTTACACGGCGGTGATTTCACACCGTTCTG
GTGAAACAGAAGATGCCACTATTGCAGATTTAGCCGTTGGTACCGCAGCAGGTCAAATCAAAACGGGTTCAATGAGCCGT
TCTGACCGTATCGCGAAATACAACCAATTAATCCGTATCGAAGAAGCATTAGGTGATAAAGCACCATTCTTAGGTTTAAA
AGCGGTTAAAGGTCAAGCATAA

Upstream 100 bases:

>100_bases
ATCAATTAATCGACATTTTTCACAAGACAAAGCAGATGAATTATTTTAAGATACGAACCATTCGTTTTAGGTTAATTTTC
ATTTAAAGAGGAAAACAAAA

Downstream 100 bases:

>100_bases
TTCGCTAATAACATCGCAAAAAAAAAGCACCGCACTTTTATCAAGTGCGGTGTTTTTTTATGAATTTCTTGAAAATGATG
ATTTTTCCTACTTAACCTGT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTGSREALELRDGDKARFLGKGVLKAVAAVNNEIAQALV
GKDATNQAEIDQIMIDLDGTENKSKFGANAILAVSLANAKAAAAAKGMPLFAWIAELNGTPGQYSMPLPMMNIINGGEHA
DNNVDIQEFMIQPVGAKTLKEALRIGAEVFHNLAKVLKGKGLSTAVGDEGGFAPNLESNAAALACIKEAVEKAGYVLGKD
VTLAMDCASSEFYNKENGMYEMKGEGKSFTSQEFTHYLEELCKEYPIVSIEDGQDESDWEGFAYQTKVLGDKVQLVGDDL
FVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSR
SDRIAKYNQLIRIEEALGDKAPFLGLKAVKGQA

Sequences:

>Translated_433_residues
MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTGSREALELRDGDKARFLGKGVLKAVAAVNNEIAQALV
GKDATNQAEIDQIMIDLDGTENKSKFGANAILAVSLANAKAAAAAKGMPLFAWIAELNGTPGQYSMPLPMMNIINGGEHA
DNNVDIQEFMIQPVGAKTLKEALRIGAEVFHNLAKVLKGKGLSTAVGDEGGFAPNLESNAAALACIKEAVEKAGYVLGKD
VTLAMDCASSEFYNKENGMYEMKGEGKSFTSQEFTHYLEELCKEYPIVSIEDGQDESDWEGFAYQTKVLGDKVQLVGDDL
FVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSR
SDRIAKYNQLIRIEEALGDKAPFLGLKAVKGQA
>Mature_432_residues
AKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTGSREALELRDGDKARFLGKGVLKAVAAVNNEIAQALVG
KDATNQAEIDQIMIDLDGTENKSKFGANAILAVSLANAKAAAAAKGMPLFAWIAELNGTPGQYSMPLPMMNIINGGEHAD
NNVDIQEFMIQPVGAKTLKEALRIGAEVFHNLAKVLKGKGLSTAVGDEGGFAPNLESNAAALACIKEAVEKAGYVLGKDV
TLAMDCASSEFYNKENGMYEMKGEGKSFTSQEFTHYLEELCKEYPIVSIEDGQDESDWEGFAYQTKVLGDKVQLVGDDLF
VTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRS
DRIAKYNQLIRIEEALGDKAPFLGLKAVKGQA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=53.5962877030162, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=53.5962877030162, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=51.2761020881671, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=51.7401392111369, Blast_Score=407, Evalue=1e-114,
Organism=Homo sapiens, GI301897479, Length=429, Percent_Identity=48.4848484848485, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=26.7857142857143, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=26.7857142857143, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=26.7857142857143, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1789141, Length=434, Percent_Identity=86.4055299539171, Blast_Score=738, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71995829, Length=433, Percent_Identity=52.8868360277136, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=433, Percent_Identity=52.8868360277136, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=46.3541666666667, Blast_Score=171, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=49.6535796766744, Blast_Score=378, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6323985, Length=435, Percent_Identity=47.1264367816092, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324974, Length=435, Percent_Identity=47.1264367816092, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324969, Length=435, Percent_Identity=47.1264367816092, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=48.9607390300231, Blast_Score=351, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24580918, Length=439, Percent_Identity=51.4806378132118, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580916, Length=439, Percent_Identity=51.4806378132118, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580920, Length=439, Percent_Identity=51.4806378132118, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580914, Length=439, Percent_Identity=51.4806378132118, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI281360527, Length=433, Percent_Identity=51.9630484988453, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI17137654, Length=433, Percent_Identity=51.9630484988453, Blast_Score=384, Evalue=1e-107,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45852; Mature: 45720

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTGSREALELRDGDKARFL
CHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAVAAVNNEIAQALVGKDATNQAEIDQIMIDLDGTENKSKFGANAILAVSLANAK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHCCCCEEEEEEECCCH
AAAAAKGMPLFAWIAELNGTPGQYSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLK
HHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHH
EALRIGAEVFHNLAKVLKGKGLSTAVGDEGGFAPNLESNAAALACIKEAVEKAGYVLGKD
HHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
VTLAMDCASSEFYNKENGMYEMKGEGKSFTSQEFTHYLEELCKEYPIVSIEDGQDESDWE
EEEEEECCCCHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCC
GFAYQTKVLGDKVQLVGDDLFVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMA
CEEEEHHHCCCEEEEECCCEEEEHHHHHHHHHHHCCCCCEEEEEHHHCHHHHHHHHHHHH
KDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDRIAKYNQLIRIEEALGDK
HCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCC
APFLGLKAVKGQA
CCCCCEECCCCCC
>Mature Secondary Structure 
AKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTGSREALELRDGDKARFL
HHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAVAAVNNEIAQALVGKDATNQAEIDQIMIDLDGTENKSKFGANAILAVSLANAK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHCCCCEEEEEEECCCH
AAAAAKGMPLFAWIAELNGTPGQYSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLK
HHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHH
EALRIGAEVFHNLAKVLKGKGLSTAVGDEGGFAPNLESNAAALACIKEAVEKAGYVLGKD
HHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
VTLAMDCASSEFYNKENGMYEMKGEGKSFTSQEFTHYLEELCKEYPIVSIEDGQDESDWE
EEEEEECCCCHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCC
GFAYQTKVLGDKVQLVGDDLFVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMA
CEEEEHHHCCCEEEEECCCEEEEHHHHHHHHHHHCCCCCEEEEEHHHCHHHHHHHHHHHH
KDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDRIAKYNQLIRIEEALGDK
HCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCC
APFLGLKAVKGQA
CCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA