Click here to switch to the map view.

The map label for this gene is yfiF [C]

Identifier: 15603722

GI number: 15603722

Start: 2099446

End: 2100525

Strand: Direct

Name: yfiF [C]

Synonym: PM1857

Alternate gene names: 15603722

Gene position: 2099446-2100525 (Clockwise)

Preceding gene: 15603720

Following gene: 15603724

Centisome position: 93.0

GC content: 45.93

Gene sequence:

>1080_bases
ATGAATAGTAAAGCGAAAGCGCCTGTCTTTCAACAAACCAGTAATAAGCAATTTCATGAACGCAGCGTTGGCGAAAAATC
TGCCAAAACTGCACCGCACTTTAAAGAAAAATTTGACGGACGTGGCGAGGCGAAGACCCGCGAAAAGTTGACGGGCAATA
AATTTTCGGCGAGAAGTAAACCTGTGCGTCGTCCACAGCAAGTCGAACAGCATATTTCTGAAACCGCCATGCGTACAGCA
TCTGGCGAAGAAGGCAAAGTGAAAGTTGTGGTGAAAAGTACGGGGGCCAGCGATAAACCTCGTGAGAAAAAAACTGGGGC
GCTTTCGCCACGCGCACCCGAAAAAATTAAGAAAAACCGTGCAGAAGAAATGAAAGTCTATGGCGAGAATGCTTGTCAGG
CATTGTTTGCACAACGTCCGGAAAGCATTGTGCGTATTTGGGCAACGGTAGAGATGGCGAAAAAGAGCGGTGAACTTTTC
AGTTATTTAGCAGAGCAGAAAAAAGCCTATCATGTGGTGGACAGCGCGGAACTCGCTTTAGTGAGCGGTACCGCGCATCA
TGGCGGCATTTGTATGTTAGTGAAAAAAGCCCGTCCTTTTACTTTAGCCGGATACTTAGACATTCCACGTAAACAAGATT
GTCTTGTGTTGATAGATGGCGTGCAAAATCCACATAATTTAGGCGGTGTTGTGCGTACTTGTGCGGTGTTTGGTGTGAAA
GGTGTGGTGATTGAACAAGCGTTAGCGGATAATTTAAACGCGCCTGCGGCAATGCGTGTGGCAGAAGGGGGAATGGAATA
TGTTCGGGTGTTGGAAACAACCGATGTGCCAAAGGCATTAGCGCAATTGCGTCAAGCAGGTTATCAACTTATTCATGTGA
CGGCGAATAAACAAGCTAAGCCGCTATCGAAATTACACTTTACTGAGAAAGTGGTGTTTGTTCTAAATGAACAAGGCGAT
GCTTCTCTTGCACAAGCCGAAGATGAACAGGTGGTGTTGTCTCTTGGTAATCCATTAAAAGCAGAATTAAATATTGCGGT
GGCAACGGGCATTTTATTAGCGCAGTGGGACTATCGTTGA

Upstream 100 bases:

>100_bases
AAAATGCGGTTTTTTCGTTATAATGCTCACACTTTCTTGCAACGCTAGGTGATTTTTTGCCCAGCTTTGCCTCCTCAACT
CATCAAATAGGCATATCGAA

Downstream 100 bases:

>100_bases
GGTTAACAGAATCGGGCATTTGCCCGATTTTTCATCATGTGCAAGGAAAAAGGTGTGGGATGTAACCTGAGGCGGGAAAA
GAAAAACGTATTCCTGCGAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MNSKAKAPVFQQTSNKQFHERSVGEKSAKTAPHFKEKFDGRGEAKTREKLTGNKFSARSKPVRRPQQVEQHISETAMRTA
SGEEGKVKVVVKSTGASDKPREKKTGALSPRAPEKIKKNRAEEMKVYGENACQALFAQRPESIVRIWATVEMAKKSGELF
SYLAEQKKAYHVVDSAELALVSGTAHHGGICMLVKKARPFTLAGYLDIPRKQDCLVLIDGVQNPHNLGGVVRTCAVFGVK
GVVIEQALADNLNAPAAMRVAEGGMEYVRVLETTDVPKALAQLRQAGYQLIHVTANKQAKPLSKLHFTEKVVFVLNEQGD
ASLAQAEDEQVVLSLGNPLKAELNIAVATGILLAQWDYR

Sequences:

>Translated_359_residues
MNSKAKAPVFQQTSNKQFHERSVGEKSAKTAPHFKEKFDGRGEAKTREKLTGNKFSARSKPVRRPQQVEQHISETAMRTA
SGEEGKVKVVVKSTGASDKPREKKTGALSPRAPEKIKKNRAEEMKVYGENACQALFAQRPESIVRIWATVEMAKKSGELF
SYLAEQKKAYHVVDSAELALVSGTAHHGGICMLVKKARPFTLAGYLDIPRKQDCLVLIDGVQNPHNLGGVVRTCAVFGVK
GVVIEQALADNLNAPAAMRVAEGGMEYVRVLETTDVPKALAQLRQAGYQLIHVTANKQAKPLSKLHFTEKVVFVLNEQGD
ASLAQAEDEQVVLSLGNPLKAELNIAVATGILLAQWDYR
>Mature_359_residues
MNSKAKAPVFQQTSNKQFHERSVGEKSAKTAPHFKEKFDGRGEAKTREKLTGNKFSARSKPVRRPQQVEQHISETAMRTA
SGEEGKVKVVVKSTGASDKPREKKTGALSPRAPEKIKKNRAEEMKVYGENACQALFAQRPESIVRIWATVEMAKKSGELF
SYLAEQKKAYHVVDSAELALVSGTAHHGGICMLVKKARPFTLAGYLDIPRKQDCLVLIDGVQNPHNLGGVVRTCAVFGVK
GVVIEQALADNLNAPAAMRVAEGGMEYVRVLETTDVPKALAQLRQAGYQLIHVTANKQAKPLSKLHFTEKVVFVLNEQGD
ASLAQAEDEQVVLSLGNPLKAELNIAVATGILLAQWDYR

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=310, Percent_Identity=39.3548387096774, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1790623, Length=238, Percent_Identity=27.3109243697479, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 39308; Mature: 39308

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKAKAPVFQQTSNKQFHERSVGEKSAKTAPHFKEKFDGRGEAKTREKLTGNKFSARSK
CCCCCCCCCHHCCCCCHHHHHHCCCHHCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCCC
PVRRPQQVEQHISETAMRTASGEEGKVKVVVKSTGASDKPREKKTGALSPRAPEKIKKNR
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
AEEMKVYGENACQALFAQRPESIVRIWATVEMAKKSGELFSYLAEQKKAYHVVDSAELAL
HHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHEEEECCCCEEE
VSGTAHHGGICMLVKKARPFTLAGYLDIPRKQDCLVLIDGVQNPHNLGGVVRTCAVFGVK
EECCCCCCCEEEEEECCCCEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCH
GVVIEQALADNLNAPAAMRVAEGGMEYVRVLETTDVPKALAQLRQAGYQLIHVTANKQAK
HHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCEEEEEEECCCCCC
PLSKLHFTEKVVFVLNEQGDASLAQAEDEQVVLSLGNPLKAELNIAVATGILLAQWDYR
CHHHHHHCEEEEEEEECCCCCHHHHCCCCEEEEECCCCCCEEEEEEEECCEEEEECCCC
>Mature Secondary Structure
MNSKAKAPVFQQTSNKQFHERSVGEKSAKTAPHFKEKFDGRGEAKTREKLTGNKFSARSK
CCCCCCCCCHHCCCCCHHHHHHCCCHHCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCCC
PVRRPQQVEQHISETAMRTASGEEGKVKVVVKSTGASDKPREKKTGALSPRAPEKIKKNR
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
AEEMKVYGENACQALFAQRPESIVRIWATVEMAKKSGELFSYLAEQKKAYHVVDSAELAL
HHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHEEEECCCCEEE
VSGTAHHGGICMLVKKARPFTLAGYLDIPRKQDCLVLIDGVQNPHNLGGVVRTCAVFGVK
EECCCCCCCEEEEEECCCCEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCH
GVVIEQALADNLNAPAAMRVAEGGMEYVRVLETTDVPKALAQLRQAGYQLIHVTANKQAK
HHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCEEEEEEECCCCCC
PLSKLHFTEKVVFVLNEQGDASLAQAEDEQVVLSLGNPLKAELNIAVATGILLAQWDYR
CHHHHHHCEEEEEEEECCCCCHHHHCCCCEEEEECCCCCCEEEEEEEECCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]