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The map label for this gene is recA

Identifier: 15603682

GI number: 15603682

Start: 2047013

End: 2048077

Strand: Reverse

Name: recA

Synonym: PM1817

Alternate gene names: 15603682

Gene position: 2048077-2047013 (Counterclockwise)

Preceding gene: 15603696

Following gene: 161723305

Centisome position: 90.72

GC content: 43.47

Gene sequence:

>1065_bases
ATGGCAACAAAAGAAGAAAAAAATAAAGCACTGGCAGCAGCACTTGGGCAGATTGAAAAACAATTTGGGAAAGGCTCTAT
TATGAAATTGGGCGATACCCAAGCCTTGGATGTTGAAGCCGTGTCAACAGGCTCCTTAAGCCTCGACGTCGCACTTGGCA
TTGGCGGGTTACCAATGGGCCGTATTGTTGAAATTTTCGGACCTGAATCTTCCGGTAAAACCACCTTAACCCTTTCTGTC
ATTGCGCAAGCACAAAAAGAAGGCAAAACCTGTGCCTTTATTGATGCCGAACACGCATTAGATCCAATTTATGCTGCCAA
ACTGGGCGTCAATGTGAATGAACTGTTAGTTTCCCAACCTGATAACGGTGAACAAGCACTCGAAATTTGCGATGCGTTAG
TGCGTTCAGGTGCAGTAGATGTGATCATTGTAGACTCAGTCGCAGCATTAACCCCGAAAGCGGAAATTGAAGGAGAAATG
GGTGACTCACACATGGGCTTACAAGCACGTTTGATGTCACAAGCATTACGTAAACTCACTGGACAAATCAAAAACTCAAA
TTGCTTAGTGGTCTTTATTAACCAAATCCGTATGAAAATTGGTGTTATGTTCGGTAACCCAGAAACCACAACAGGGGGTA
ATGCACTGAAATTCTATGCCTCTGTACGTTTAGATATTCGCCGTACGGGTGCGATTAAAGAGGGTGAAGAAGTGATCGGT
AACGAAACCCGTGTCAAAGTGGTGAAAAACAAAGTGGCGGCACCATTCCGTCAAGTAGATTTCCAAATTCTTTACGGTCA
AGGTATTTCTAAAACAGGTGAGCTCATCGAGTTAGGCGTAAAACACAAATTAGTGGATAAAGCAGGTGCATGGTACGCTT
ACAATGGTGAAAAAATCGGTCAAGGTAAAGCTAACGCGATGAAATGGTTAGAAGAGCATCCTGAAGAAGCATTGGCTTTA
GAAACGAAATTACGCAATGAGTTGCTCGCGAATCCAGAAAAAGTACTCGCGGCAGACATTGCGGAAAAAAATGAAAGCTC
TACGGGCTTAGAAGCGGATTATTAA

Upstream 100 bases:

>100_bases
GAGAAATCCCACAAATTTGATAAAAAGCACTTGATACTGTTTATTCATACAGTTAATATACACGACAAATTCTGATGATG
ATTTTATTGAGGATTCAAAC

Downstream 100 bases:

>100_bases
TCTGTTTTTAGCGGTTTAATCGAAAAAAGTGCGGTGAGTTTTAACAAAATTTCACCGCACTTTTTGCTGAGTGATATCTA
AACATTGTAAAGAAGCAGGT

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MATKEEKNKALAAALGQIEKQFGKGSIMKLGDTQALDVEAVSTGSLSLDVALGIGGLPMGRIVEIFGPESSGKTTLTLSV
IAQAQKEGKTCAFIDAEHALDPIYAAKLGVNVNELLVSQPDNGEQALEICDALVRSGAVDVIIVDSVAALTPKAEIEGEM
GDSHMGLQARLMSQALRKLTGQIKNSNCLVVFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGEEVIG
NETRVKVVKNKVAAPFRQVDFQILYGQGISKTGELIELGVKHKLVDKAGAWYAYNGEKIGQGKANAMKWLEEHPEEALAL
ETKLRNELLANPEKVLAADIAEKNESSTGLEADY

Sequences:

>Translated_354_residues
MATKEEKNKALAAALGQIEKQFGKGSIMKLGDTQALDVEAVSTGSLSLDVALGIGGLPMGRIVEIFGPESSGKTTLTLSV
IAQAQKEGKTCAFIDAEHALDPIYAAKLGVNVNELLVSQPDNGEQALEICDALVRSGAVDVIIVDSVAALTPKAEIEGEM
GDSHMGLQARLMSQALRKLTGQIKNSNCLVVFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGEEVIG
NETRVKVVKNKVAAPFRQVDFQILYGQGISKTGELIELGVKHKLVDKAGAWYAYNGEKIGQGKANAMKWLEEHPEEALAL
ETKLRNELLANPEKVLAADIAEKNESSTGLEADY
>Mature_353_residues
ATKEEKNKALAAALGQIEKQFGKGSIMKLGDTQALDVEAVSTGSLSLDVALGIGGLPMGRIVEIFGPESSGKTTLTLSVI
AQAQKEGKTCAFIDAEHALDPIYAAKLGVNVNELLVSQPDNGEQALEICDALVRSGAVDVIIVDSVAALTPKAEIEGEMG
DSHMGLQARLMSQALRKLTGQIKNSNCLVVFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGEEVIGN
ETRVKVVKNKVAAPFRQVDFQILYGQGISKTGELIELGVKHKLVDKAGAWYAYNGEKIGQGKANAMKWLEEHPEEALALE
TKLRNELLANPEKVLAADIAEKNESSTGLEADY

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family

Homologues:

Organism=Escherichia coli, GI1789051, Length=351, Percent_Identity=75.4985754985755, Blast_Score=548, Evalue=1e-157,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RECA_PASMU (P95526)

Other databases:

- EMBL:   X99324
- EMBL:   AE004439
- RefSeq:   NP_246756.1
- ProteinModelPortal:   P95526
- PRIDE:   P95526
- GeneID:   1245164
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1817
- NMPDR:   fig|272843.1.peg.1818
- HOGENOM:   HBG339889
- OMA:   YGEGINT
- ProtClustDB:   PRK09354
- BioCyc:   PMUL272843:PM1817-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00268
- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587
- PANTHER:   PTHR22942:SF1
- PRINTS:   PR00142
- SMART:   SM00382
- TIGRFAMs:   TIGR02012

Pfam domain/function: PF00154 RecA

EC number: NA

Molecular weight: Translated: 37907; Mature: 37776

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00321 RECA_1; PS50162 RECA_2; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKEEKNKALAAALGQIEKQFGKGSIMKLGDTQALDVEAVSTGSLSLDVALGIGGLPMG
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCEEEEEEECCCCCCHH
RIVEIFGPESSGKTTLTLSVIAQAQKEGKTCAFIDAEHALDPIYAAKLGVNVNELLVSQP
HEEEEECCCCCCCEEEEHHHHHHHHHCCCEEEEEEHHHCCCCHHHHHHCCCHHHHEEECC
DNGEQALEICDALVRSGAVDVIIVDSVAALTPKAEIEGEMGDSHMGLQARLMSQALRKLT
CCHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHH
GQIKNSNCLVVFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGEEVIG
HHCCCCCEEEEEEEEEEEEEEEEECCCCCCCCCCEEEEEEEEEEEEEECCCCCCCHHHHC
NETRVKVVKNKVAAPFRQVDFQILYGQGISKTGELIELGVKHKLVDKAGAWYAYNGEKIG
CCCEEEEHHHHHCCCHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCC
QGKANAMKWLEEHPEEALALETKLRNELLANPEKVLAADIAEKNESSTGLEADY
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ATKEEKNKALAAALGQIEKQFGKGSIMKLGDTQALDVEAVSTGSLSLDVALGIGGLPMG
CCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCEEEEEEECCCCCCHH
RIVEIFGPESSGKTTLTLSVIAQAQKEGKTCAFIDAEHALDPIYAAKLGVNVNELLVSQP
HEEEEECCCCCCCEEEEHHHHHHHHHCCCEEEEEEHHHCCCCHHHHHHCCCHHHHEEECC
DNGEQALEICDALVRSGAVDVIIVDSVAALTPKAEIEGEMGDSHMGLQARLMSQALRKLT
CCHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHH
GQIKNSNCLVVFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTGAIKEGEEVIG
HHCCCCCEEEEEEEEEEEEEEEEECCCCCCCCCCEEEEEEEEEEEEEECCCCCCCHHHHC
NETRVKVVKNKVAAPFRQVDFQILYGQGISKTGELIELGVKHKLVDKAGAWYAYNGEKIG
CCCEEEEHHHHHCCCHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCC
QGKANAMKWLEEHPEEALALETKLRNELLANPEKVLAADIAEKNESSTGLEADY
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100