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The map label for this gene is dsbA [H]

Identifier: 15603666

GI number: 15603666

Start: 2025945

End: 2026565

Strand: Direct

Name: dsbA [H]

Synonym: PM1801

Alternate gene names: 15603666

Gene position: 2025945-2026565 (Clockwise)

Preceding gene: 15603665

Following gene: 15603667

Centisome position: 89.74

GC content: 39.61

Gene sequence:

>621_bases
ATGAAAAAATTAGTTTTAGCGGTGACGTCATTCCTTTTTGCTGTTTCTGTACAAGCGACAAATTTAACCGAGGGTAAGCA
GTATGTGACGTTAAATCAGGCACCTGTACAACAAGCAGAAGTCATCGAGTTTTTCTCTTTTTATTGTCCACATTGTTATT
CGTTTGAATACGAATACCAAATCCCAAATAAAGTGAAACAGCAGTTGCCAGAAGGTGTGAGCTTGAAACAATATCATGTC
AACTTTTTAGGCGGTGAAATGGGGAAAAATCTTACTCGTGCATGGGCATTGGCTATGGCGACCGGTGTACAAGATAAAGT
AAAAGAACCTTTATTTTTCGCGGCACAGCAAAATAAATTACGCAGTATGGATGATATTCGTCAAATCTTTTTGGCTAATG
GTCTCAGTGCAGAACAATTTGATGGCGGAATTAACAGTTTTGCCGTCACAGCGTTAACCAATAAGCAAGTGACAGCGGCT
GAACACATGAAAGTGCGTGGTGTGCCAGATTTTTACGTAAACGGGCGTTATCGTGTTAATCCTGAAGGACTGAAAAACGA
CAGCCATGAAGCGTTTGTTGCTGATTATGTCGAAACAGTAAAAGGTTTATTGCAAAAATAA

Upstream 100 bases:

>100_bases
GGATAAAAATGCGCCGATCCCGGGTATCAAGAAAGATTACGAAGATGATTTTAAGACTGCGTTGCTGAAAGCGCGTGGCA
TTATTAAATAAGGATTTGCA

Downstream 100 bases:

>100_bases
CAAAAAATTGGTTTAATGCTTCGTTTTTTGAACGAATAGCTTATTGTAGAGGATATTATGGCAGAAAGTTTTAGTGTTAC
ACGTCGTTTTTTTGATGATA

Product: DsbA

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MKKLVLAVTSFLFAVSVQATNLTEGKQYVTLNQAPVQQAEVIEFFSFYCPHCYSFEYEYQIPNKVKQQLPEGVSLKQYHV
NFLGGEMGKNLTRAWALAMATGVQDKVKEPLFFAAQQNKLRSMDDIRQIFLANGLSAEQFDGGINSFAVTALTNKQVTAA
EHMKVRGVPDFYVNGRYRVNPEGLKNDSHEAFVADYVETVKGLLQK

Sequences:

>Translated_206_residues
MKKLVLAVTSFLFAVSVQATNLTEGKQYVTLNQAPVQQAEVIEFFSFYCPHCYSFEYEYQIPNKVKQQLPEGVSLKQYHV
NFLGGEMGKNLTRAWALAMATGVQDKVKEPLFFAAQQNKLRSMDDIRQIFLANGLSAEQFDGGINSFAVTALTNKQVTAA
EHMKVRGVPDFYVNGRYRVNPEGLKNDSHEAFVADYVETVKGLLQK
>Mature_206_residues
MKKLVLAVTSFLFAVSVQATNLTEGKQYVTLNQAPVQQAEVIEFFSFYCPHCYSFEYEYQIPNKVKQQLPEGVSLKQYHV
NFLGGEMGKNLTRAWALAMATGVQDKVKEPLFFAAQQNKLRSMDDIRQIFLANGLSAEQFDGGINSFAVTALTNKQVTAA
EHMKVRGVPDFYVNGRYRVNPEGLKNDSHEAFVADYVETVKGLLQK

Specific function: Involved in disulfide-bond formation. Required for HI transformation. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=207, Percent_Identity=46.8599033816425, Blast_Score=211, Evalue=3e-56,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 23159; Mature: 23159

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVLAVTSFLFAVSVQATNLTEGKQYVTLNQAPVQQAEVIEFFSFYCPHCYSFEYEYQ
CCHHHHHHHHHHHHHHHEECCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEE
IPNKVKQQLPEGVSLKQYHVNFLGGEMGKNLTRAWALAMATGVQDKVKEPLFFAAQQNKL
CCHHHHHHCCCCCCCHHHHHHHHCHHHCHHHHHHHHHHHHHCCHHHHHCCEEEHHHHHHH
RSMDDIRQIFLANGLSAEQFDGGINSFAVTALTNKQVTAAEHMKVRGVPDFYVNGRYRVN
CCHHHHHHHHHHCCCCHHHHCCCCCCEEEEEECCCCCHHHHHEEECCCCCEEECCEEEEC
PEGLKNDSHEAFVADYVETVKGLLQK
CCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLVLAVTSFLFAVSVQATNLTEGKQYVTLNQAPVQQAEVIEFFSFYCPHCYSFEYEYQ
CCHHHHHHHHHHHHHHHEECCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEE
IPNKVKQQLPEGVSLKQYHVNFLGGEMGKNLTRAWALAMATGVQDKVKEPLFFAAQQNKL
CCHHHHHHCCCCCCCHHHHHHHHCHHHCHHHHHHHHHHHHHCCHHHHHCCEEEHHHHHHH
RSMDDIRQIFLANGLSAEQFDGGINSFAVTALTNKQVTAAEHMKVRGVPDFYVNGRYRVN
CCHHHHHHHHHHCCCCHHHHCCCCCCEEEEEECCCCCHHHHHEEECCCCCEEECCEEEEC
PEGLKNDSHEAFVADYVETVKGLLQK
CCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1438213; 7542800 [H]