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The map label for this gene is ligA

Identifier: 15603581

GI number: 15603581

Start: 1923922

End: 1925943

Strand: Reverse

Name: ligA

Synonym: PM1716

Alternate gene names: 15603581

Gene position: 1925943-1923922 (Counterclockwise)

Preceding gene: 15603583

Following gene: 15603574

Centisome position: 85.31

GC content: 44.61

Gene sequence:

>2022_bases
ATGACAGACAGTATTAAATTAGAAATAGAACAATTACGCCAAACGCTACGTTATCATGAATATCAATATCATGTCTTAGA
TAATCCACAAATTCCTGATGCGGAATACGATCGTTTATTTCATCGTTTAAAAACGCTGGAACAACAATATCCACAATGGT
TTAGCCCGGACTCTCCTACACAACGAGTGGGCGCGAAACCCCTTTCTGCGTTTGCGCAAGTGCAACATGAAATGCCTATG
CTGTCTTTGGATAATGCATTTTCAGATGAAGAGTTGCATGCTTTTGTCAAACGTATTCAAGACCGTCTGGTTTTTTCCCC
TAAATTACTTGAATTTTGCTGCGAACCGAAATTAGATGGGTTGGCGGTCAGTATTTTGTATGTAGATGGAAAATTGACAC
AAGCAGCGACCCGTGGTGATGGGAGTACAGGAGAAGACATTACGTTAAATATTCGCACAGTACGTAATATCCCTTTGCAA
TTGTTAATGGAAAATCCACCGACGCGTTTGGAAGTGCGTGGAGAAGTCTTTATGTCGCAAGCGGGCTTTGAGGTATTAAA
TGAAAAAGCGTTAGCCAGAGGGGAGAAGACCTTTGCCAATCCACGTAATGCGGCAGCGGGATCGTTGCGTCAGTTAGATC
CGCGAATTACTAGCCAACGTCCGTTGCTATTAAACGCATATAGCATTGGTGTCGCAGAGGGGATTGATTTACCTGATACC
CATTTTGAACGCTTACAATGGTTAAAATCCATTGGTATTCCGGTGAATAATGAGATTCAGTTATGTGAAGGGACGGAAAA
TGTGCTCAATTTCTACCGCGCGATTATGCAAAAACGGAGCACTTTGGGCTATGACATTGATGGGACAGTGATTAAAGTCA
ACGATATTGCTTTACAAGAGGAATTAGGTTTTATTTCGAAAGCGCCACGTTGGGCAATTGCCTATAAATTCCCAGCACAA
GAAGAATTGACAGTATTAAATGCGGTGGAGTTTCAAGTGGGGAGAACCGGTGCAATTACACCCGTCGCGAAGTTACAACC
TGTGTTCGTCGCAGGCGTGACGGTCAGTAATGCGACACTGCATAATGGCGATGAAATTGCACGTTTGGATGTGGCAATTG
GTGATACGGTGATTATTCGCCGCGCGGGAGATGTGATTCCACAAATTATTGGTGTGCTACATGAAAAACGCCCAGCCAAT
GCAGAGAAGATCGTTTTTCCGACAGAATGTCCAGTTTGCGGTTCGGTGATTGTACGTATTGAAGGTGAGGCCGTTGCCCG
TTGTACGGGCGGTTTATTCTGTGCGGCACAACGTAAAGAAGCATTGAAACATTTCGTCTCACGTAAAGCCATGGATATTG
ATGGTGTTGGGGCAAAACTTATTGAACAGTTGGTGGACAGAGAACAAATTCATACCCCAGCGGATTTATTTAAATTGGAT
CTCAACACGTTGGCTCGTTTAGAGCGCATGGGGCTTAAATCTGCGCAAAATGCCCTCGACAGTTTACAGAAAGCGAAAAA
AACGACTTTAGCGCGTTTTATTTTTGCGCTAGGTATTCGTGAAGTGGGCGAGGCCACCGCACTCAACTTAGCCAATCATT
TTAAGACCTTAGAGGCATTAAAAGAAGCGACGCTGGAGCAACTACAAGAAGTGCAAGATGTGGGCGAAGTGGTCGCTAAT
CGGATTTTTGTGTTCTGGCGTGAACCGCATAATGTGGCAGTAGTCGAGGATCTGATTGCGCAAGGCATCCATTGGGAAAC
CGTAGAAGTCAAAGATGTTGGAGATAACCCATTTAAAGAGAAAACAGTGGTGTTAACTGGCACCTTAACGCAAATGGGGC
GGACTGAAGCGAAAGCCTTACTCCAGCAGTTAGGAGCAAAAGTCAGTGGCAGTGTTTCTGCCAAAACGGATCTGGTGGTG
GCAGGTGACAGCGCAGGTTCAAAACTGACAAAAGCCAATGAGCTTGGTGTCAAAGTGATTGATGAAAACACCTTCTTGGC
GTGGAGCAAACCGTACTTGTAA

Upstream 100 bases:

>100_bases
AAAATAATAGGTGTTAAATTTCTTTAATCAATAGGCTCAAAGTATTGTAAAATTACGCGTCGAATCTGCAAAAGTGCGGT
GATTTTTATCAGGATTTTAT

Downstream 100 bases:

>100_bases
GATGAAATAAAACCGTTTAGCGTTAACTAAACGGTTTTTTCTCTTTTTTGACCGCACTTGACACATCAAAGTGCGGTTGC
TTTTTTCCAAAATTAAGAAA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MTDSIKLEIEQLRQTLRYHEYQYHVLDNPQIPDAEYDRLFHRLKTLEQQYPQWFSPDSPTQRVGAKPLSAFAQVQHEMPM
LSLDNAFSDEELHAFVKRIQDRLVFSPKLLEFCCEPKLDGLAVSILYVDGKLTQAATRGDGSTGEDITLNIRTVRNIPLQ
LLMENPPTRLEVRGEVFMSQAGFEVLNEKALARGEKTFANPRNAAAGSLRQLDPRITSQRPLLLNAYSIGVAEGIDLPDT
HFERLQWLKSIGIPVNNEIQLCEGTENVLNFYRAIMQKRSTLGYDIDGTVIKVNDIALQEELGFISKAPRWAIAYKFPAQ
EELTVLNAVEFQVGRTGAITPVAKLQPVFVAGVTVSNATLHNGDEIARLDVAIGDTVIIRRAGDVIPQIIGVLHEKRPAN
AEKIVFPTECPVCGSVIVRIEGEAVARCTGGLFCAAQRKEALKHFVSRKAMDIDGVGAKLIEQLVDREQIHTPADLFKLD
LNTLARLERMGLKSAQNALDSLQKAKKTTLARFIFALGIREVGEATALNLANHFKTLEALKEATLEQLQEVQDVGEVVAN
RIFVFWREPHNVAVVEDLIAQGIHWETVEVKDVGDNPFKEKTVVLTGTLTQMGRTEAKALLQQLGAKVSGSVSAKTDLVV
AGDSAGSKLTKANELGVKVIDENTFLAWSKPYL

Sequences:

>Translated_673_residues
MTDSIKLEIEQLRQTLRYHEYQYHVLDNPQIPDAEYDRLFHRLKTLEQQYPQWFSPDSPTQRVGAKPLSAFAQVQHEMPM
LSLDNAFSDEELHAFVKRIQDRLVFSPKLLEFCCEPKLDGLAVSILYVDGKLTQAATRGDGSTGEDITLNIRTVRNIPLQ
LLMENPPTRLEVRGEVFMSQAGFEVLNEKALARGEKTFANPRNAAAGSLRQLDPRITSQRPLLLNAYSIGVAEGIDLPDT
HFERLQWLKSIGIPVNNEIQLCEGTENVLNFYRAIMQKRSTLGYDIDGTVIKVNDIALQEELGFISKAPRWAIAYKFPAQ
EELTVLNAVEFQVGRTGAITPVAKLQPVFVAGVTVSNATLHNGDEIARLDVAIGDTVIIRRAGDVIPQIIGVLHEKRPAN
AEKIVFPTECPVCGSVIVRIEGEAVARCTGGLFCAAQRKEALKHFVSRKAMDIDGVGAKLIEQLVDREQIHTPADLFKLD
LNTLARLERMGLKSAQNALDSLQKAKKTTLARFIFALGIREVGEATALNLANHFKTLEALKEATLEQLQEVQDVGEVVAN
RIFVFWREPHNVAVVEDLIAQGIHWETVEVKDVGDNPFKEKTVVLTGTLTQMGRTEAKALLQQLGAKVSGSVSAKTDLVV
AGDSAGSKLTKANELGVKVIDENTFLAWSKPYL
>Mature_672_residues
TDSIKLEIEQLRQTLRYHEYQYHVLDNPQIPDAEYDRLFHRLKTLEQQYPQWFSPDSPTQRVGAKPLSAFAQVQHEMPML
SLDNAFSDEELHAFVKRIQDRLVFSPKLLEFCCEPKLDGLAVSILYVDGKLTQAATRGDGSTGEDITLNIRTVRNIPLQL
LMENPPTRLEVRGEVFMSQAGFEVLNEKALARGEKTFANPRNAAAGSLRQLDPRITSQRPLLLNAYSIGVAEGIDLPDTH
FERLQWLKSIGIPVNNEIQLCEGTENVLNFYRAIMQKRSTLGYDIDGTVIKVNDIALQEELGFISKAPRWAIAYKFPAQE
ELTVLNAVEFQVGRTGAITPVAKLQPVFVAGVTVSNATLHNGDEIARLDVAIGDTVIIRRAGDVIPQIIGVLHEKRPANA
EKIVFPTECPVCGSVIVRIEGEAVARCTGGLFCAAQRKEALKHFVSRKAMDIDGVGAKLIEQLVDREQIHTPADLFKLDL
NTLARLERMGLKSAQNALDSLQKAKKTTLARFIFALGIREVGEATALNLANHFKTLEALKEATLEQLQEVQDVGEVVANR
IFVFWREPHNVAVVEDLIAQGIHWETVEVKDVGDNPFKEKTVVLTGTLTQMGRTEAKALLQQLGAKVSGSVSAKTDLVVA
GDSAGSKLTKANELGVKVIDENTFLAWSKPYL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=667, Percent_Identity=62.8185907046477, Blast_Score=867, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=583, Percent_Identity=22.1269296740995, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_PASMU (Q9CKA9)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246655.1
- HSSP:   O87703
- ProteinModelPortal:   Q9CKA9
- GeneID:   1245063
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1716
- NMPDR:   fig|272843.1.peg.1717
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   PMUL272843:PM1716-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74611; Mature: 74479

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 117-117 BINDING 115-115 BINDING 138-138 BINDING 175-175 BINDING 292-292 BINDING 316-316

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSIKLEIEQLRQTLRYHEYQYHVLDNPQIPDAEYDRLFHRLKTLEQQYPQWFSPDSPT
CCCCCEEEHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCH
QRVGAKPLSAFAQVQHEMPMLSLDNAFSDEELHAFVKRIQDRLVFSPKLLEFCCEPKLDG
HHHCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
LAVSILYVDGKLTQAATRGDGSTGEDITLNIRTVRNIPLQLLMENPPTRLEVRGEVFMSQ
EEEEEEEECCCHHHHHCCCCCCCCCEEEEEEEEECCCCEEEEECCCCCEEEECHHHHHHH
AGFEVLNEKALARGEKTFANPRNAAAGSLRQLDPRITSQRPLLLNAYSIGVAEGIDLPDT
HHHHHHHHHHHHCCCHHCCCCCCHHCCCHHHCCCCCCCCCCEEEEEEECCHHCCCCCCCH
HFERLQWLKSIGIPVNNEIQLCEGTENVLNFYRAIMQKRSTLGYDIDGTVIKVNDIALQE
HHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHH
ELGFISKAPRWAIAYKFPAQEELTVLNAVEFQVGRTGAITPVAKLQPVFVAGVTVSNATL
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEEEECCEE
HNGDEIARLDVAIGDTVIIRRAGDVIPQIIGVLHEKRPANAEKIVFPTECPVCGSVIVRI
CCCCCEEEEEEEECCEEEEECHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEE
EGEAVARCTGGLFCAAQRKEALKHFVSRKAMDIDGVGAKLIEQLVDREQIHTPADLFKLD
CCCCEEHHCCCEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
LNTLARLERMGLKSAQNALDSLQKAKKTTLARFIFALGIREVGEATALNLANHFKTLEAL
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KEATLEQLQEVQDVGEVVANRIFVFWREPHNVAVVEDLIAQGIHWETVEVKDVGDNPFKE
HHHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCC
KTVVLTGTLTQMGRTEAKALLQQLGAKVSGSVSAKTDLVVAGDSAGSKLTKANELGVKVI
CEEEEEECHHHHCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCHHCCCCEEE
DENTFLAWSKPYL
ECCEEEEECCCCC
>Mature Secondary Structure 
TDSIKLEIEQLRQTLRYHEYQYHVLDNPQIPDAEYDRLFHRLKTLEQQYPQWFSPDSPT
CCCCEEEHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCH
QRVGAKPLSAFAQVQHEMPMLSLDNAFSDEELHAFVKRIQDRLVFSPKLLEFCCEPKLDG
HHHCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
LAVSILYVDGKLTQAATRGDGSTGEDITLNIRTVRNIPLQLLMENPPTRLEVRGEVFMSQ
EEEEEEEECCCHHHHHCCCCCCCCCEEEEEEEEECCCCEEEEECCCCCEEEECHHHHHHH
AGFEVLNEKALARGEKTFANPRNAAAGSLRQLDPRITSQRPLLLNAYSIGVAEGIDLPDT
HHHHHHHHHHHHCCCHHCCCCCCHHCCCHHHCCCCCCCCCCEEEEEEECCHHCCCCCCCH
HFERLQWLKSIGIPVNNEIQLCEGTENVLNFYRAIMQKRSTLGYDIDGTVIKVNDIALQE
HHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHH
ELGFISKAPRWAIAYKFPAQEELTVLNAVEFQVGRTGAITPVAKLQPVFVAGVTVSNATL
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEEEECCEE
HNGDEIARLDVAIGDTVIIRRAGDVIPQIIGVLHEKRPANAEKIVFPTECPVCGSVIVRI
CCCCCEEEEEEEECCEEEEECHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEE
EGEAVARCTGGLFCAAQRKEALKHFVSRKAMDIDGVGAKLIEQLVDREQIHTPADLFKLD
CCCCEEHHCCCEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
LNTLARLERMGLKSAQNALDSLQKAKKTTLARFIFALGIREVGEATALNLANHFKTLEAL
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KEATLEQLQEVQDVGEVVANRIFVFWREPHNVAVVEDLIAQGIHWETVEVKDVGDNPFKE
HHHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCC
KTVVLTGTLTQMGRTEAKALLQQLGAKVSGSVSAKTDLVVAGDSAGSKLTKANELGVKVI
CEEEEEECHHHHCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCHHCCCCEEE
DENTFLAWSKPYL
ECCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100