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The map label for this gene is hemB [H]

Identifier: 15603557

GI number: 15603557

Start: 1900218

End: 1901243

Strand: Direct

Name: hemB [H]

Synonym: PM1692

Alternate gene names: 15603557

Gene position: 1900218-1901243 (Clockwise)

Preceding gene: 15603556

Following gene: 15603560

Centisome position: 84.17

GC content: 43.08

Gene sequence:

>1026_bases
ATGACACAACAAATTTTTGCCAATTTCCCTCAACGTCGCCTACGCCGTATGCGGAAACAAGCTTTCAGTCGCCGTTTAAT
GGCAGAAAATAAACTCAGCGTAGAGGATTTAATTTATCCCGTTTTTATTATTGAAGGCGAAAAGCAAAGGGAACCCGTAC
CTTCCATGCCTGGTGTAGAACGCTTAACCATTGATGAGCTATTAATTGAAGCCGAACTTTTAGTCAAATATGGGGTACCG
GTGATTGCGCTTTTCCCGGTGATTGCACAAGAGAAAAAATCCTTAATGGCAGAAGAAGCTTATCATCCTGATGGCTTAGT
ACAGCGCGCGGTAAAAGCGTTAAAGGACAAATTCCCAACTGAACTTGGCATACTTACTGATATTGCCCTCGATCCCTATA
CTACACACGGACAAGACGGGATAATTGATCAACATGGCTATGTGTTAAATGATGTCACTAGCGATATTTTGGTCAAACAA
GCGCTTTCCCATGCGAAAGCGGGCGCAGATATTGTAGCACCAAGCGATATGATGGACGGTAGAATTGGCAAAATTCGCCA
TGCCCTCGAGGAAAAGGGGTTTATCAATACACTCATTATGGCGTATTCTGCCAAATATGCTTCTCATTATTATGGACCTT
TCCGCGATGCCGTCGGTTCTGCCACCAATTTAAAAGGGGGCGATAAAAAAACTTATCAATTAGATCCGGCCAATAGTGAT
GAAGGACTACAAGAAGTGGCATTAGATTTACAAGAAGGGGCAGATATGGTGATGGTCAAACCCGGCATGCCGTATTTAGA
TTTAGTTTATCGCGTGAAAACCCATTTTGGCGTGCCAACTTTCGCTTACCAAGTATCGGGTGAGTATGCCATGCATCAAG
CTGCGATCCAAAACGGCTGGCTCAAAGAAAAAGAATGTATTATGGAATCTTTATTGTGCTTCAAACGTGCTGGCGCCGAT
GGGATTTTGACGTATTACGCCAAACAAGTAGCAGAATGGCTATATTGGGAAAAACAACATCAATAA

Upstream 100 bases:

>100_bases
GCCCTAGAGAGGATGAAGAAACGCAATAGTTCACGAGGCAGACCAACGTGTCTGCCTTATTTTTAGCGCGAAGACTGACT
TAAACACGAGGACGCTATTC

Downstream 100 bases:

>100_bases
TGTCTCTTGCAATAAAAAGTGCGGTCAAAATTTCATACGTTTTGACCGCACTTTGCTTGTAATAAAGGGAAGGACTTAAG
CCTCAATGCCTTCAAATAAT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MTQQIFANFPQRRLRRMRKQAFSRRLMAENKLSVEDLIYPVFIIEGEKQREPVPSMPGVERLTIDELLIEAELLVKYGVP
VIALFPVIAQEKKSLMAEEAYHPDGLVQRAVKALKDKFPTELGILTDIALDPYTTHGQDGIIDQHGYVLNDVTSDILVKQ
ALSHAKAGADIVAPSDMMDGRIGKIRHALEEKGFINTLIMAYSAKYASHYYGPFRDAVGSATNLKGGDKKTYQLDPANSD
EGLQEVALDLQEGADMVMVKPGMPYLDLVYRVKTHFGVPTFAYQVSGEYAMHQAAIQNGWLKEKECIMESLLCFKRAGAD
GILTYYAKQVAEWLYWEKQHQ

Sequences:

>Translated_341_residues
MTQQIFANFPQRRLRRMRKQAFSRRLMAENKLSVEDLIYPVFIIEGEKQREPVPSMPGVERLTIDELLIEAELLVKYGVP
VIALFPVIAQEKKSLMAEEAYHPDGLVQRAVKALKDKFPTELGILTDIALDPYTTHGQDGIIDQHGYVLNDVTSDILVKQ
ALSHAKAGADIVAPSDMMDGRIGKIRHALEEKGFINTLIMAYSAKYASHYYGPFRDAVGSATNLKGGDKKTYQLDPANSD
EGLQEVALDLQEGADMVMVKPGMPYLDLVYRVKTHFGVPTFAYQVSGEYAMHQAAIQNGWLKEKECIMESLLCFKRAGAD
GILTYYAKQVAEWLYWEKQHQ
>Mature_340_residues
TQQIFANFPQRRLRRMRKQAFSRRLMAENKLSVEDLIYPVFIIEGEKQREPVPSMPGVERLTIDELLIEAELLVKYGVPV
IALFPVIAQEKKSLMAEEAYHPDGLVQRAVKALKDKFPTELGILTDIALDPYTTHGQDGIIDQHGYVLNDVTSDILVKQA
LSHAKAGADIVAPSDMMDGRIGKIRHALEEKGFINTLIMAYSAKYASHYYGPFRDAVGSATNLKGGDKKTYQLDPANSDE
GLQEVALDLQEGADMVMVKPGMPYLDLVYRVKTHFGVPTFAYQVSGEYAMHQAAIQNGWLKEKECIMESLLCFKRAGADG
ILTYYAKQVAEWLYWEKQHQ

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=309, Percent_Identity=40.453074433657, Blast_Score=228, Evalue=6e-60,
Organism=Escherichia coli, GI87081728, Length=321, Percent_Identity=41.7445482866044, Blast_Score=231, Evalue=6e-62,
Organism=Saccharomyces cerevisiae, GI6321398, Length=315, Percent_Identity=36.8253968253968, Blast_Score=186, Evalue=7e-48,
Organism=Drosophila melanogaster, GI21358291, Length=318, Percent_Identity=38.3647798742138, Blast_Score=214, Evalue=7e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 38390; Mature: 38259

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQIFANFPQRRLRRMRKQAFSRRLMAENKLSVEDLIYPVFIIEGEKQREPVPSMPGVE
CCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEEEEEEECCHHCCCCCCCCCCC
RLTIDELLIEAELLVKYGVPVIALFPVIAQEKKSLMAEEAYHPDGLVQRAVKALKDKFPT
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCH
ELGILTDIALDPYTTHGQDGIIDQHGYVLNDVTSDILVKQALSHAKAGADIVAPSDMMDG
HHHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCC
RIGKIRHALEEKGFINTLIMAYSAKYASHYYGPFRDAVGSATNLKGGDKKTYQLDPANSD
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEECCCCCC
EGLQEVALDLQEGADMVMVKPGMPYLDLVYRVKTHFGVPTFAYQVSGEYAMHQAAIQNGW
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCC
LKEKECIMESLLCFKRAGADGILTYYAKQVAEWLYWEKQHQ
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQQIFANFPQRRLRRMRKQAFSRRLMAENKLSVEDLIYPVFIIEGEKQREPVPSMPGVE
CCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEEEEEEECCHHCCCCCCCCCCC
RLTIDELLIEAELLVKYGVPVIALFPVIAQEKKSLMAEEAYHPDGLVQRAVKALKDKFPT
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCH
ELGILTDIALDPYTTHGQDGIIDQHGYVLNDVTSDILVKQALSHAKAGADIVAPSDMMDG
HHHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCC
RIGKIRHALEEKGFINTLIMAYSAKYASHYYGPFRDAVGSATNLKGGDKKTYQLDPANSD
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEECCCCCC
EGLQEVALDLQEGADMVMVKPGMPYLDLVYRVKTHFGVPTFAYQVSGEYAMHQAAIQNGW
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCC
LKEKECIMESLLCFKRAGADGILTYYAKQVAEWLYWEKQHQ
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]