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The map label for this gene is nhaA

Identifier: 15603549

GI number: 15603549

Start: 1893034

End: 1894173

Strand: Direct

Name: nhaA

Synonym: PM1684

Alternate gene names: 15603549

Gene position: 1893034-1894173 (Clockwise)

Preceding gene: 15603548

Following gene: 15603550

Centisome position: 83.86

GC content: 42.81

Gene sequence:

>1140_bases
ATGTTAACACCAATTCAAAACTTTCTGAAACAAGAAGCGGCAGGTGGTATTCTGCTGTTCATTTTTGCAACCTTAGCCAT
TATTTTAGCCAATACCCCATTAAGTTATTTATACTTTGATTTTTTGCAAACACCAGTGAGTGTCCAAATTGGTGCATTCA
TCATCAATAAACCGCTTTTAATGTGGGTCAACGATGGTCTTATGGCCGTGTTTTTCATGCTCGTCGGAATGGAAGTTAAA
CGTGAATTACTAGAAGGCTCGTTATCCAGTTATCAACGTGCTGTTTTTCCCGCCATTGCGGCAACAGGCGGTATGGTTGT
TCCTGCAATTGTCTTTCTTGTGTTTAACGCAACACATCCTGAATTTCAAGAGGGATGGGCGATCCCGATGGCAACGGATA
TCGCTTTTGCCCTTGGCGTCATCGCCTTATTAGGTAAACGTGTTCCACTCGCGCTGAAAATTTTCTTGTTGGCACTGGCG
ATTATTGATGACTTAGGGGCGATTGTTGTTATTGCGCTATTTTTCTCTCATGATTTAAGTCCACAAGCCTTTATCTTCGC
GGGGATTGCTGTGGCAATTTTGATCACAATGAACCGTTTAAAAATTACTGCACTCTCGGCTTACGGCATTGTCGGTATTA
TTCTTTGGGCATCCGTACTCAAATCAGGTGTACACGCCACTTTGGCGGGAGTGATTATCGGTTTTTGTATCCCATTAAAC
GGTAAGAAAGGCGAGCGCCCACTAGATGACTTTGAACATACTTTATCACCTTGGAGTGCCTTTGCGATTCTCCCACTGTT
TGCTTTCTGTAATGCTGGGGTTTCATTAATTGGTATGGGAATGGATAACTTAACCTCTACGTTACCAATGGGGATTGCCC
TTGGCTTACTCTTAGGTAAACCTCTGGGGATTTTCTCATTCTGTTTTGTGGCAGTAAAATTAGGCATTGCTAAGCTGTCT
GAAGGCATTAATTTCAAACAAATTTTTGCTGTGTCTGTACTCTGTGGAATTGGATTTACCATGTCCATGTTCCTTGCTGG
TCTCGCCTTTGGTGGAGAAAGTGATAGCGAAAATGTCACTGCACTCGCCCGTTTAGGTATTCTGATTGGTTCTGGTTTCT
CCGCGGTGTTAGGTTATTGA

Upstream 100 bases:

>100_bases
AAATGAAAAATTACGACGTAATCGATCATTTTATTTCATTTTTCAGCACAAAAAGTGATATAAATATAATCATCATAAAA
AAAAGATATTGAGGGGAAAA

Downstream 100 bases:

>100_bases
CTGTTGAAAATGTCCACAAAAGACCCCATTGAGGCACAATAAATCAAATAAAAAACACCACCAACAAATATAAGAGTGGT
GTTTTTTTATCCATTAGAAA

Product: pH-dependent sodium/proton antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MLTPIQNFLKQEAAGGILLFIFATLAIILANTPLSYLYFDFLQTPVSVQIGAFIINKPLLMWVNDGLMAVFFMLVGMEVK
RELLEGSLSSYQRAVFPAIAATGGMVVPAIVFLVFNATHPEFQEGWAIPMATDIAFALGVIALLGKRVPLALKIFLLALA
IIDDLGAIVVIALFFSHDLSPQAFIFAGIAVAILITMNRLKITALSAYGIVGIILWASVLKSGVHATLAGVIIGFCIPLN
GKKGERPLDDFEHTLSPWSAFAILPLFAFCNAGVSLIGMGMDNLTSTLPMGIALGLLLGKPLGIFSFCFVAVKLGIAKLS
EGINFKQIFAVSVLCGIGFTMSMFLAGLAFGGESDSENVTALARLGILIGSGFSAVLGY

Sequences:

>Translated_379_residues
MLTPIQNFLKQEAAGGILLFIFATLAIILANTPLSYLYFDFLQTPVSVQIGAFIINKPLLMWVNDGLMAVFFMLVGMEVK
RELLEGSLSSYQRAVFPAIAATGGMVVPAIVFLVFNATHPEFQEGWAIPMATDIAFALGVIALLGKRVPLALKIFLLALA
IIDDLGAIVVIALFFSHDLSPQAFIFAGIAVAILITMNRLKITALSAYGIVGIILWASVLKSGVHATLAGVIIGFCIPLN
GKKGERPLDDFEHTLSPWSAFAILPLFAFCNAGVSLIGMGMDNLTSTLPMGIALGLLLGKPLGIFSFCFVAVKLGIAKLS
EGINFKQIFAVSVLCGIGFTMSMFLAGLAFGGESDSENVTALARLGILIGSGFSAVLGY
>Mature_379_residues
MLTPIQNFLKQEAAGGILLFIFATLAIILANTPLSYLYFDFLQTPVSVQIGAFIINKPLLMWVNDGLMAVFFMLVGMEVK
RELLEGSLSSYQRAVFPAIAATGGMVVPAIVFLVFNATHPEFQEGWAIPMATDIAFALGVIALLGKRVPLALKIFLLALA
IIDDLGAIVVIALFFSHDLSPQAFIFAGIAVAILITMNRLKITALSAYGIVGIILWASVLKSGVHATLAGVIIGFCIPLN
GKKGERPLDDFEHTLSPWSAFAILPLFAFCNAGVSLIGMGMDNLTSTLPMGIALGLLLGKPLGIFSFCFVAVKLGIAKLS
EGINFKQIFAVSVLCGIGFTMSMFLAGLAFGGESDSENVTALARLGILIGSGFSAVLGY

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family

Homologues:

Organism=Escherichia coli, GI1786201, Length=379, Percent_Identity=58.311345646438, Blast_Score=398, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAA_PASMU (Q9CKD8)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246623.1
- HSSP:   P13738
- ProteinModelPortal:   Q9CKD8
- SMR:   Q9CKD8
- GeneID:   1245031
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1684
- NMPDR:   fig|272843.1.peg.1685
- HOGENOM:   HBG665619
- OMA:   LIHWIND
- ProtClustDB:   PRK09560
- BioCyc:   PMUL272843:PM1684-MONOMER
- HAMAP:   MF_01844
- InterPro:   IPR023171
- InterPro:   IPR004670
- TIGRFAMs:   TIGR00773

Pfam domain/function: PF06965 Na_H_antiport_1

EC number: NA

Molecular weight: Translated: 40190; Mature: 40190

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xecf89ac)-; HASH(0xeaf1e08)-; HASH(0xebb3bac)-; HASH(0xe8fff04)-; HASH(0xd0c3998)-; HASH(0x8b17138)-; HASH(0xd289484)-; HASH(0xe9a8254)-; HASH(0xeacbf70)-; HASH(0xf254ca0)-; HASH(0xe9afce4)-; HASH(0x8b1a2dc)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPIQNFLKQEAAGGILLFIFATLAIILANTPLSYLYFDFLQTPVSVQIGAFIINKPLL
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCEE
MWVNDGLMAVFFMLVGMEVKRELLEGSLSSYQRAVFPAIAATGGMVVPAIVFLVFNATHP
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
EFQEGWAIPMATDIAFALGVIALLGKRVPLALKIFLLALAIIDDLGAIVVIALFFSHDLS
CHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PQAFIFAGIAVAILITMNRLKITALSAYGIVGIILWASVLKSGVHATLAGVIIGFCIPLN
CHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GKKGERPLDDFEHTLSPWSAFAILPLFAFCNAGVSLIGMGMDNLTSTLPMGIALGLLLGK
CCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCHHEEECCHHHHHHHHHHHHHHHHHHCC
PLGIFSFCFVAVKLGIAKLSEGINFKQIFAVSVLCGIGFTMSMFLAGLAFGGESDSENVT
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
ALARLGILIGSGFSAVLGY
HHHHHHHHHCCCHHHHHCH
>Mature Secondary Structure
MLTPIQNFLKQEAAGGILLFIFATLAIILANTPLSYLYFDFLQTPVSVQIGAFIINKPLL
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCEE
MWVNDGLMAVFFMLVGMEVKRELLEGSLSSYQRAVFPAIAATGGMVVPAIVFLVFNATHP
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
EFQEGWAIPMATDIAFALGVIALLGKRVPLALKIFLLALAIIDDLGAIVVIALFFSHDLS
CHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PQAFIFAGIAVAILITMNRLKITALSAYGIVGIILWASVLKSGVHATLAGVIIGFCIPLN
CHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GKKGERPLDDFEHTLSPWSAFAILPLFAFCNAGVSLIGMGMDNLTSTLPMGIALGLLLGK
CCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCHHEEECCHHHHHHHHHHHHHHHHHHCC
PLGIFSFCFVAVKLGIAKLSEGINFKQIFAVSVLCGIGFTMSMFLAGLAFGGESDSENVT
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
ALARLGILIGSGFSAVLGY
HHHHHHHHHCCCHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11248100