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The map label for this gene is truD

Identifier: 15603475

GI number: 15603475

Start: 1820362

End: 1821369

Strand: Direct

Name: truD

Synonym: PM1610

Alternate gene names: 15603475

Gene position: 1820362-1821369 (Clockwise)

Preceding gene: 15603474

Following gene: 15603476

Centisome position: 80.64

GC content: 45.83

Gene sequence:

>1008_bases
ATGGAACTGGCTTATTTACATACTCGTCCAGAGCAATACGCACGTTTGAAAGCCGAATGTGCGGATTTTATTGTCAAAGA
AAACCTCGGTTATGAAATGTCTGGCGACGGGGAATTTGTGGCGGTAAAAGTACGCAAAACGGATTGCAACACGCTGTTTG
TCGGTGAAAAACTCGCCCAATTCGTCGGTATTTCTGAACGTAATATGGGTTATGCGGGCTTAAAAGATCGCAAAGCCGTG
ACAGAGCAATGGTTCTGCTTACACATGCCGGGGCAACCCACACCTGATTTCCGCTCATTCCAGTTAGAGGGGGTCGATAT
CCTCGAAGTGACGCGCCATCATCGCAAAATTCGTACGGGGAGTCTTGAGGGGAATCATTTTGAGATTTTGCTACGTGGTG
CAAAAGAGACCGATGAACTCAACGTGCGTTTAAACAATATTAAACAATGTGGTTTTCCAAACTATTTTACCGAGCAGCGT
TTTGGGCGCGATGGGCATAACCTGACGCAAGCCTTACGTTGGGCACAAGGTGAAATTAACGTCAAAGATCGCAAAAAACG
CAGTTTTTATCTTTCCGCTGCACGCAGTGAAGTCTTCAATCTTGTCGTATCAGAACGCATTGCATTACAGCTTGCGCAAC
AAGTACTACGTGGCGATATGTTACAACTACAAGGCTCACACAGTTGGTTTCAAGCCGACGAAAAAGAAGATCTGAATGCC
TTACAAGCCCGTTTAGAACAACAAGATATTTTACTCACGGCACCGCTAATTGGTGAACAAAATCCTCCTGCCACTGACAT
TGAAAATCAATTGGTTGAACAACATCAAGCCTTGTTAACCCTGATGGCGAAAGAACGTATGAAAGCGGCTCGTCGCCCAA
TGTTAATGCAAGCGCAAGCGTTGCAATGGGCATTTGTTGCAGAAGGACTCAAACTCGCCTTCTACTTACCCGCGGGCAGT
TATGCCACTGCCTTGGTGAGAGAAGTGGTGAATGTGCAAGAAGATTAA

Upstream 100 bases:

>100_bases
AACAAGTGAACGTGAAAGCGACCACCAGTGAACGTTTAGGCTTTACCGGACGCGGTGAAGGCATTGCCTGTGAAGCTGTG
GCGTTAGTGGTGAAAAGTTA

Downstream 100 bases:

>100_bases
TTTCAACCCAACATTTAAAAGGAATGGCTATGCAAAGATGGAGCAAAACACTGCTCAGTATTATGTTGCTTGTGAGTACA
ATGAATGTCTTCGCTCAACG

Product: tRNA pseudouridine synthase D

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MELAYLHTRPEQYARLKAECADFIVKENLGYEMSGDGEFVAVKVRKTDCNTLFVGEKLAQFVGISERNMGYAGLKDRKAV
TEQWFCLHMPGQPTPDFRSFQLEGVDILEVTRHHRKIRTGSLEGNHFEILLRGAKETDELNVRLNNIKQCGFPNYFTEQR
FGRDGHNLTQALRWAQGEINVKDRKKRSFYLSAARSEVFNLVVSERIALQLAQQVLRGDMLQLQGSHSWFQADEKEDLNA
LQARLEQQDILLTAPLIGEQNPPATDIENQLVEQHQALLTLMAKERMKAARRPMLMQAQALQWAFVAEGLKLAFYLPAGS
YATALVREVVNVQED

Sequences:

>Translated_335_residues
MELAYLHTRPEQYARLKAECADFIVKENLGYEMSGDGEFVAVKVRKTDCNTLFVGEKLAQFVGISERNMGYAGLKDRKAV
TEQWFCLHMPGQPTPDFRSFQLEGVDILEVTRHHRKIRTGSLEGNHFEILLRGAKETDELNVRLNNIKQCGFPNYFTEQR
FGRDGHNLTQALRWAQGEINVKDRKKRSFYLSAARSEVFNLVVSERIALQLAQQVLRGDMLQLQGSHSWFQADEKEDLNA
LQARLEQQDILLTAPLIGEQNPPATDIENQLVEQHQALLTLMAKERMKAARRPMLMQAQALQWAFVAEGLKLAFYLPAGS
YATALVREVVNVQED
>Mature_335_residues
MELAYLHTRPEQYARLKAECADFIVKENLGYEMSGDGEFVAVKVRKTDCNTLFVGEKLAQFVGISERNMGYAGLKDRKAV
TEQWFCLHMPGQPTPDFRSFQLEGVDILEVTRHHRKIRTGSLEGNHFEILLRGAKETDELNVRLNNIKQCGFPNYFTEQR
FGRDGHNLTQALRWAQGEINVKDRKKRSFYLSAARSEVFNLVVSERIALQLAQQVLRGDMLQLQGSHSWFQADEKEDLNA
LQARLEQQDILLTAPLIGEQNPPATDIENQLVEQHQALLTLMAKERMKAARRPMLMQAQALQWAFVAEGLKLAFYLPAGS
YATALVREVVNVQED

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain

Homologues:

Organism=Escherichia coli, GI1789102, Length=338, Percent_Identity=44.9704142011834, Blast_Score=290, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUD_PASMU (Q9CKK4)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246549.1
- ProteinModelPortal:   Q9CKK4
- SMR:   Q9CKK4
- GeneID:   1244957
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1610
- NMPDR:   fig|272843.1.peg.1611
- HOGENOM:   HBG541221
- OMA:   ALTRQWF
- ProtClustDB:   PRK00984
- BioCyc:   PMUL272843:PM1610-MONOMER
- HAMAP:   MF_01082
- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760
- PANTHER:   PTHR13326
- TIGRFAMs:   TIGR00094

Pfam domain/function: PF01142 TruD; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 38225; Mature: 38225

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50984 TRUD; PS01268 UPF0024

Important sites: ACT_SITE 76-76

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELAYLHTRPEQYARLKAECADFIVKENLGYEMSGDGEFVAVKVRKTDCNTLFVGEKLAQ
CCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEEECCCCEEEHHHHHHH
FVGISERNMGYAGLKDRKAVTEQWFCLHMPGQPTPDFRSFQLEGVDILEVTRHHRKIRTG
HHCCCCCCCCCCCCCHHHHHHHCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHCCC
SLEGNHFEILLRGAKETDELNVRLNNIKQCGFPNYFTEQRFGRDGHNLTQALRWAQGEIN
CCCCCEEEEEEECCCCCCEEEEEEECHHHCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCC
VKDRKKRSFYLSAARSEVFNLVVSERIALQLAQQVLRGDMLQLQGSHSWFQADEKEDLNA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHH
LQARLEQQDILLTAPLIGEQNPPATDIENQLVEQHQALLTLMAKERMKAARRPMLMQAQA
HHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LQWAFVAEGLKLAFYLPAGSYATALVREVVNVQED
HHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MELAYLHTRPEQYARLKAECADFIVKENLGYEMSGDGEFVAVKVRKTDCNTLFVGEKLAQ
CCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEEECCCCEEEHHHHHHH
FVGISERNMGYAGLKDRKAVTEQWFCLHMPGQPTPDFRSFQLEGVDILEVTRHHRKIRTG
HHCCCCCCCCCCCCCHHHHHHHCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHCCC
SLEGNHFEILLRGAKETDELNVRLNNIKQCGFPNYFTEQRFGRDGHNLTQALRWAQGEIN
CCCCCEEEEEEECCCCCCEEEEEEECHHHCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCC
VKDRKKRSFYLSAARSEVFNLVVSERIALQLAQQVLRGDMLQLQGSHSWFQADEKEDLNA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHH
LQARLEQQDILLTAPLIGEQNPPATDIENQLVEQHQALLTLMAKERMKAARRPMLMQAQA
HHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LQWAFVAEGLKLAFYLPAGSYATALVREVVNVQED
HHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100