Click here to switch to the map view.

The map label for this gene is ispF

Identifier: 15603474

GI number: 15603474

Start: 1819886

End: 1820362

Strand: Direct

Name: ispF

Synonym: PM1609

Alternate gene names: 15603474

Gene position: 1819886-1820362 (Clockwise)

Preceding gene: 15603473

Following gene: 15603475

Centisome position: 80.62

GC content: 46.96

Gene sequence:

>477_bases
ATGATACGAATTGGTCATGGTTTTGATGTACACGCCTTTGGTGGCGAAGGTCCAATCATTATTGGCGGCGTGGCAATTCC
TTATGAAAAAGGGTTACTTGCTCATTCTGATGGTGATGTGGCATTACATGCTTTAACCGATGCGCTACTTGGTGCGGTCG
CATTAGGTGATATTGGCAAACTGTTCCCAGATACTGACATGCAATACAAAGGCGCAGATAGCCGTGGGTTATTGCGCGAA
GCTTACACACAAGTACAAGCCAAAGGTTACAAAGTGGGTAATGTAGATGTCACTATCATCGCACAAGCCCCGAAAATGCG
TCCGCATATTGATGCGATGCGAGCGGCAATTGCCGAAGATTTAGCCTGTGATATTGAACAAGTGAACGTGAAAGCGACCA
CCAGTGAACGTTTAGGCTTTACCGGACGCGGTGAAGGCATTGCCTGTGAAGCTGTGGCGTTAGTGGTGAAAAGTTAA

Upstream 100 bases:

>100_bases
CGCACTTAGTGGCAGGGCGAAGCGATAATATTAAAGTGACCCGTCCAGAAGATTTCGCGTTAGCAGAATTTTATTTAAGC
AGAACAAAATAGGAAAAAAG

Downstream 100 bases:

>100_bases
TGGAACTGGCTTATTTACATACTCGTCCAGAGCAATACGCACGTTTGAAAGCCGAATGTGCGGATTTTATTGTCAAAGAA
AACCTCGGTTATGAAATGTC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MIRIGHGFDVHAFGGEGPIIIGGVAIPYEKGLLAHSDGDVALHALTDALLGAVALGDIGKLFPDTDMQYKGADSRGLLRE
AYTQVQAKGYKVGNVDVTIIAQAPKMRPHIDAMRAAIAEDLACDIEQVNVKATTSERLGFTGRGEGIACEAVALVVKS

Sequences:

>Translated_158_residues
MIRIGHGFDVHAFGGEGPIIIGGVAIPYEKGLLAHSDGDVALHALTDALLGAVALGDIGKLFPDTDMQYKGADSRGLLRE
AYTQVQAKGYKVGNVDVTIIAQAPKMRPHIDAMRAAIAEDLACDIEQVNVKATTSERLGFTGRGEGIACEAVALVVKS
>Mature_158_residues
MIRIGHGFDVHAFGGEGPIIIGGVAIPYEKGLLAHSDGDVALHALTDALLGAVALGDIGKLFPDTDMQYKGADSRGLLRE
AYTQVQAKGYKVGNVDVTIIAQAPKMRPHIDAMRAAIAEDLACDIEQVNVKATTSERLGFTGRGEGIACEAVALVVKS

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=82.1656050955414, Blast_Score=240, Evalue=3e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_PASMU (P57954)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246548.1
- ProteinModelPortal:   P57954
- SMR:   P57954
- GeneID:   1244956
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1609
- NMPDR:   fig|272843.1.peg.1610
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   PMUL272843:PM1609-MONOMER
- BRENDA:   4.6.1.12
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16555; Mature: 16555

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIGHGFDVHAFGGEGPIIIGGVAIPYEKGLLAHSDGDVALHALTDALLGAVALGDIGK
CEEECCCCEEEEECCCCCEEEEEEECCCCCCEEEECCCCEEHHHHHHHHHHHHHHHHHHH
LFPDTDMQYKGADSRGLLREAYTQVQAKGYKVGNVDVTIIAQAPKMRPHIDAMRAAIAED
HCCCCCCEECCCCCCHHHHHHHHHHHHCCEEECCEEEEEEEECCCCCCHHHHHHHHHHHH
LACDIEQVNVKATTSERLGFTGRGEGIACEAVALVVKS
HHCCHHHEEEEEECCCCCCCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MIRIGHGFDVHAFGGEGPIIIGGVAIPYEKGLLAHSDGDVALHALTDALLGAVALGDIGK
CEEECCCCEEEEECCCCCEEEEEEECCCCCCEEEECCCCEEHHHHHHHHHHHHHHHHHHH
LFPDTDMQYKGADSRGLLREAYTQVQAKGYKVGNVDVTIIAQAPKMRPHIDAMRAAIAED
HCCCCCCEECCCCCCHHHHHHHHHHHHCCEEECCEEEEEEEECCCCCCHHHHHHHHHHHH
LACDIEQVNVKATTSERLGFTGRGEGIACEAVALVVKS
HHCCHHHEEEEEECCCCCCCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100