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The map label for this gene is ispD

Identifier: 15603473

GI number: 15603473

Start: 1819161

End: 1819877

Strand: Direct

Name: ispD

Synonym: PM1608

Alternate gene names: 15603473

Gene position: 1819161-1819877 (Clockwise)

Preceding gene: 15603472

Following gene: 15603474

Centisome position: 80.58

GC content: 45.19

Gene sequence:

>717_bases
ATGACAACGCCGATTGCTAAATCTACACGTCGTATTGTTGCTGTTGTGCCTGCGGCGGGCATAGGTAGCCGCATGCAAAT
GGATAAACCGAAGCAGTATCTGCACATTCATGGCAAAACGATCTTAGAACATACTTTATCTGTGCTGTTAGGTTATCCAT
TAATTGAAAAAATTATTTTAGCGGTGGCAGCAAACGATCCCTATATTTCCACTTGCCCTTTATTAACACACCCGAAAATT
CAGCTGGTCGAAGGGGGCAGCAGTCGTGCTGACTCAGTGCTCAATGGGCTTAACGCGGTCAAAAGTGCGGTACAAAATAG
CGAAGATTTTTGGGTGATGGTGCATGATGCGGCACGCCCTTGTTTAACACATCAAGATTTAGATAAATTGGTGCAAGTGG
AAGATCAAAACGGGGCGATTCTGGCTATTCCCGCCACCGATACCATTAAACGTGCTTTACACAATCAGCAAATTCATTAT
ACCGAAGATCGCTCGCAACTTTGGCTAGCGCAGACGCCACAATTTTTTCCCATAGCAACCTTAGCACAAGCCTTAGAACA
GGCATTGGCGCAAGGTTTACAGGTGACGGATGAAGCCTCTGCCATGGAATTTGCTGGATTTCGACCGCACTTAGTGGCAG
GGCGAAGCGATAATATTAAAGTGACCCGTCCAGAAGATTTCGCGTTAGCAGAATTTTATTTAAGCAGAACAAAATAG

Upstream 100 bases:

>100_bases
TAAAACAAGGGGTAGAAGCTATCGAAGAACGTGCCAGATTTCAGCATGATATGGTGAAACCCGATGAAATTTTTTATCAC
ATTGTCAAAGAGCAGAAATA

Downstream 100 bases:

>100_bases
GAAAAAAGATGATACGAATTGGTCATGGTTTTGATGTACACGCCTTTGGTGGCGAAGGTCCAATCATTATTGGCGGCGTG
GCAATTCCTTATGAAAAAGG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MTTPIAKSTRRIVAVVPAAGIGSRMQMDKPKQYLHIHGKTILEHTLSVLLGYPLIEKIILAVAANDPYISTCPLLTHPKI
QLVEGGSSRADSVLNGLNAVKSAVQNSEDFWVMVHDAARPCLTHQDLDKLVQVEDQNGAILAIPATDTIKRALHNQQIHY
TEDRSQLWLAQTPQFFPIATLAQALEQALAQGLQVTDEASAMEFAGFRPHLVAGRSDNIKVTRPEDFALAEFYLSRTK

Sequences:

>Translated_238_residues
MTTPIAKSTRRIVAVVPAAGIGSRMQMDKPKQYLHIHGKTILEHTLSVLLGYPLIEKIILAVAANDPYISTCPLLTHPKI
QLVEGGSSRADSVLNGLNAVKSAVQNSEDFWVMVHDAARPCLTHQDLDKLVQVEDQNGAILAIPATDTIKRALHNQQIHY
TEDRSQLWLAQTPQFFPIATLAQALEQALAQGLQVTDEASAMEFAGFRPHLVAGRSDNIKVTRPEDFALAEFYLSRTK
>Mature_237_residues
TTPIAKSTRRIVAVVPAAGIGSRMQMDKPKQYLHIHGKTILEHTLSVLLGYPLIEKIILAVAANDPYISTCPLLTHPKIQ
LVEGGSSRADSVLNGLNAVKSAVQNSEDFWVMVHDAARPCLTHQDLDKLVQVEDQNGAILAIPATDTIKRALHNQQIHYT
EDRSQLWLAQTPQFFPIATLAQALEQALAQGLQVTDEASAMEFAGFRPHLVAGRSDNIKVTRPEDFALAEFYLSRTK

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=228, Percent_Identity=30.2631578947368, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI157671913, Length=133, Percent_Identity=33.0827067669173, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789104, Length=229, Percent_Identity=52.4017467248908, Blast_Score=236, Evalue=9e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_PASMU (P57953)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246547.1
- ProteinModelPortal:   P57953
- SMR:   P57953
- GeneID:   1244955
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1608
- NMPDR:   fig|272843.1.peg.1609
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- ProtClustDB:   PRK00155
- BioCyc:   PMUL272843:PM1608-MONOMER
- BRENDA:   2.7.7.60
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 26195; Mature: 26064

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPIAKSTRRIVAVVPAAGIGSRMQMDKPKQYLHIHGKTILEHTLSVLLGYPLIEKIIL
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHH
AVAANDPYISTCPLLTHPKIQLVEGGSSRADSVLNGLNAVKSAVQNSEDFWVMVHDAARP
HHCCCCCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
CLTHQDLDKLVQVEDQNGAILAIPATDTIKRALHNQQIHYTEDRSQLWLAQTPQFFPIAT
CCCHHHHHHHHEEECCCCEEEEECCHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCHHH
LAQALEQALAQGLQVTDEASAMEFAGFRPHLVAGRSDNIKVTRPEDFALAEFYLSRTK
HHHHHHHHHHCCCCCCCCCCHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TTPIAKSTRRIVAVVPAAGIGSRMQMDKPKQYLHIHGKTILEHTLSVLLGYPLIEKIIL
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHH
AVAANDPYISTCPLLTHPKIQLVEGGSSRADSVLNGLNAVKSAVQNSEDFWVMVHDAARP
HHCCCCCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
CLTHQDLDKLVQVEDQNGAILAIPATDTIKRALHNQQIHYTEDRSQLWLAQTPQFFPIAT
CCCHHHHHHHHEEECCCCEEEEECCHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCHHH
LAQALEQALAQGLQVTDEASAMEFAGFRPHLVAGRSDNIKVTRPEDFALAEFYLSRTK
HHHHHHHHHHCCCCCCCCCCHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100