Click here to switch to the map view.

The map label for this gene is tagI [H]

Identifier: 15603466

GI number: 15603466

Start: 1813399

End: 1813950

Strand: Reverse

Name: tagI [H]

Synonym: PM1601

Alternate gene names: 15603466

Gene position: 1813950-1813399 (Counterclockwise)

Preceding gene: 15603468

Following gene: 15603465

Centisome position: 80.35

GC content: 40.58

Gene sequence:

>552_bases
ATGAAAACACGTTGCAGTTGGGTAGGTAATAGCACAATTTATCGTGATTATCACGATCACGAATGGGGCAAACCAGAATA
TGATAGTCTCAAATTATTTGAAAAAATTTGCCTAGAAGGGCAGCAAGCTGGCTTATCTTGGCTGACGGTCTTAAAAAAAC
GTGAGAATTACCGCCACGCTTTTCATCAATTTAACCCAGAAAAAATCGCGAAGATGACCACACTTGATGTGGATAAGTTA
ATGCAGGATCAAGGACTCATTCGTCATCGCAAAAAACTTGAAGCGATTATTACTAACGCCAAAGCGTATTTAGCCATGCA
AAAGTGCGGTGAGAATTTTAGCGATTTTATTTGGTCGTTTGTCAATCACCAACCCCAGTTCAATGACGTCCCTAACTTAG
CAAGCGTCCCCACGAAAACACCAACTTCCATTGCTATGTCTAACGCGTTGAAAAAACGGGGCTTTGTTTTTGTTGGAGAA
ACCACTTGTTATGCCTTTATGCAGTCGATGGGATTGGTCAATGATCACCTGAATGATTGTTGCTGTAAATAA

Upstream 100 bases:

>100_bases
ATCAAAAGGAAAATGGAATGATCTTTTCTTGCACTGTTGCTAAGCAAACGCTTGCTTGAAAAGACAGAAAAAAGAGAGCG
TACTGACAAAGGAGAAACAC

Downstream 100 bases:

>100_bases
TATGCCTTTCCCCTGATTTTCCAGTATAATCAGGCTAATTTTTGATTATGTATGGTTTATCCGATGAAAAAGAATTCTTA
CACACGCCTTTCTATCGCTA

Product: TagI

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase; TAG [H]

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MKTRCSWVGNSTIYRDYHDHEWGKPEYDSLKLFEKICLEGQQAGLSWLTVLKKRENYRHAFHQFNPEKIAKMTTLDVDKL
MQDQGLIRHRKKLEAIITNAKAYLAMQKCGENFSDFIWSFVNHQPQFNDVPNLASVPTKTPTSIAMSNALKKRGFVFVGE
TTCYAFMQSMGLVNDHLNDCCCK

Sequences:

>Translated_183_residues
MKTRCSWVGNSTIYRDYHDHEWGKPEYDSLKLFEKICLEGQQAGLSWLTVLKKRENYRHAFHQFNPEKIAKMTTLDVDKL
MQDQGLIRHRKKLEAIITNAKAYLAMQKCGENFSDFIWSFVNHQPQFNDVPNLASVPTKTPTSIAMSNALKKRGFVFVGE
TTCYAFMQSMGLVNDHLNDCCCK
>Mature_183_residues
MKTRCSWVGNSTIYRDYHDHEWGKPEYDSLKLFEKICLEGQQAGLSWLTVLKKRENYRHAFHQFNPEKIAKMTTLDVDKL
MQDQGLIRHRKKLEAIITNAKAYLAMQKCGENFSDFIWSFVNHQPQFNDVPNLASVPTKTPTSIAMSNALKKRGFVFVGE
TTCYAFMQSMGLVNDHLNDCCCK

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=179, Percent_Identity=64.2458100558659, Blast_Score=254, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 21164; Mature: 21164

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRCSWVGNSTIYRDYHDHEWGKPEYDSLKLFEKICLEGQQAGLSWLTVLKKRENYRHA
CCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
FHQFNPEKIAKMTTLDVDKLMQDQGLIRHRKKLEAIITNAKAYLAMQKCGENFSDFIWSF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VNHQPQFNDVPNLASVPTKTPTSIAMSNALKKRGFVFVGETTCYAFMQSMGLVNDHLNDC
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCHHHHHHHHH
CCK
HCC
>Mature Secondary Structure
MKTRCSWVGNSTIYRDYHDHEWGKPEYDSLKLFEKICLEGQQAGLSWLTVLKKRENYRHA
CCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
FHQFNPEKIAKMTTLDVDKLMQDQGLIRHRKKLEAIITNAKAYLAMQKCGENFSDFIWSF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VNHQPQFNDVPNLASVPTKTPTSIAMSNALKKRGFVFVGETTCYAFMQSMGLVNDHLNDC
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCHHHHHHHHH
CCK
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]