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The map label for this gene is lptD [H]

Identifier: 15603465

GI number: 15603465

Start: 1810987

End: 1813347

Strand: Reverse

Name: lptD [H]

Synonym: PM1600

Alternate gene names: 15603465

Gene position: 1813347-1810987 (Counterclockwise)

Preceding gene: 15603466

Following gene: 15603456

Centisome position: 80.33

GC content: 41.63

Gene sequence:

>2361_bases
ATGGTTTATCCGATGAAAAAGAATTCTTACACACGCCTTTCTATCGCTATCTTGAGTACCCTTTATAGTGTATCCAGTTT
AGCTGACTTGAAATCACAATGTTTAGCTGGTGTCCCACATTTTACGGGCGAATTGATCACGGGTAATCCAAATGACTTGC
CTGTTTACATCGAAGCTGATCAGGCAAGATTAAACCAATCTGTTAGTGCCAGCTATGAAGGCAATGTAGAAATTCAACAA
GGTAACCGCCAACTCCGTGCCCAACGTGCAGACATCAGTCAACAAAAACAGCCCAATGGATTACAGCGTTTAGCGCATGT
CCGTGGAGGATTTGAGTATCAAGATCATCAAATCCAGCTAAAAGGTGAAGAAGCGCAAGTGCAGCTCAATACGAAAAACA
CTGATATCAAAAACGCAGATTACCAACTAGTCGATCGTCAAGGACGAGGCAAAGCAGAATCCATTGCGCTACGCGAAGAT
TATCGTCTCATGACAAATGCGGTGTTTACATCTTGCTTACCACAAGATAATGCATGGTCAATTGAAGCGAAAGAAATGCG
ACAACATATTCAAGAAGAATACGCGGAAATGTGGCATGCACGTTTTAAAGTGCATGGTGTACCGATTTTTTACACCCCTT
ATTTACAGCTACCGATTGGGGATCGCCGTCGTTCAGGTTTATTATTACCAAATTTTGGGACGTCAAATCGTGACGGTTAT
TTCTATGAGCAACCGTTTTATTGGAATATTGCACCAAACCTTGATGCCACCATGACCCCCAAATATATGTCAAAACGTGG
TTGGCAACTTAATGGTGAATTCCGCTATTTAAGCCCAATCGGGGAAGGAAAAATTGCAGGCGAATATTTAAAGCAAGATC
GACTAACCGATTACCGCAATCGCGATCGTTCTCGCCACTTATTTTATTGGACACATCATTCCAGTTTCTTACAAAATTGG
CGTTTAAATTTGGATTATACCCGTGTTAGCGATCAACGTTATTTTTCAGACTTTGATTCAGCTTATGGCTCAAGCACCGA
CGGCTATGCGGATCAAAAATTCCGTGTTGCCTATTACCAACCGCATTACAATATTGCGATTTCGGCTAAACAATTCCAAA
TTTTCAACGAAGTGGATATTGGTCCATACCGCGCTCTGCCGCAAGTCGATTTCAACTATTACCAAAATGCCCTGTTCGAT
AGCCCTTTGAATTTCAAACTCTTTTCACAAGTAGTTCACTTTGATAATAAAAGTCCATTAATGCCAAAAGCATGGCGTTT
TCATGCTGAACCGAGCATCAATTTACCGCTATCGAATCGTTATGGTAGTTTAAACATTGAAACGAAACTCTATGCAACCC
ACTACCAACAAACGCAAGGGCGTGCGGCAGAAGCAGAACAAGTAGAACGAAAAATCAATCGTGTCTTGCCACAAGTCAAA
GTGGACTTACAAACGGTTTTGGCTAGCCAGCAAACGTTTATTGAAGGTTACACACAAACTTTAGAACCTCATGTGCAATA
TTTATATCGCCCTTACAAAGACCAAAGCAATATTGGTTCAAAATTAAATAATGCCTACTTAGGTTTTGGTTATGACTCTT
CTCTCTTACAGCAAGACTATTTCGGCTTATTCCGTGATCGTCGTTACAGTGGATTAGACCGCATCGCCTCCGCCAATCAA
TTCACGGTAGGTGGTACCACGCGTATTTATGATAAAAAAGGCAATGAACGCTTTAATTTCTCTGCGGGGCAAATTTACTA
CTTGCAAGACTCGCGCATTGATAACAGTAAAGAAAACAGCACCTATGGGCGCTCCTCCTCTTGGTCACTTGCCTCCAACT
GGAAAATCAATGATCAATGGCGTTGGCAAGGCAGCTATCAATATGATACACGCTTAAATAAGTCCTCATTGGGCAACTTT
ACTCTAGAATATAACCCAACGGGAAATAATATTATTCAATTAAGTTACCGTTATGCAAGTCAACAATATATTGACCAAAA
CCTCACCTCAGCGGCAAACCGCTACGGACAAGACATAAAACAGCTTGGTCTAACTGTGGCATGGGCGCTCACGGATCAAT
GGGCGGTGGTAGCACGTCATTATCAAGATCTTGCTTTGAGAAAGCCTGTTGAGCAATATTTGGGACTAGAATACAGCACT
TGCTGTTGGGCGATTAATGTTGGTGCCCGACGTAGTGTGACCAGCAAAGAAAACCAAACGGCGAATCAAATTTTTTATGA
CAAAAGTATTGGTATCAATATTGAGCTACGGGGCTTAGGTCGTGATGATCACAAAGGCAATATTGAGAAAATGCTACAAC
GAGGCAAACTACCGTATTTACAAGCGTTTAGCCTCTATTAA

Upstream 100 bases:

>100_bases
GTCGATGGGATTGGTCAATGATCACCTGAATGATTGTTGCTGTAAATAATATGCCTTTCCCCTGATTTTCCAGTATAATC
AGGCTAATTTTTGATTATGT

Downstream 100 bases:

>100_bases
TCTCATGAAACTGCCGTCTCGTGTCATATGAGATAATATACTGCAAGGACTGATGTTGATAGCCGTTTCAACACAGTCCT
TTCTCATTTGTGGTTACTCT

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 786; Mature: 786

Protein sequence:

>786_residues
MVYPMKKNSYTRLSIAILSTLYSVSSLADLKSQCLAGVPHFTGELITGNPNDLPVYIEADQARLNQSVSASYEGNVEIQQ
GNRQLRAQRADISQQKQPNGLQRLAHVRGGFEYQDHQIQLKGEEAQVQLNTKNTDIKNADYQLVDRQGRGKAESIALRED
YRLMTNAVFTSCLPQDNAWSIEAKEMRQHIQEEYAEMWHARFKVHGVPIFYTPYLQLPIGDRRRSGLLLPNFGTSNRDGY
FYEQPFYWNIAPNLDATMTPKYMSKRGWQLNGEFRYLSPIGEGKIAGEYLKQDRLTDYRNRDRSRHLFYWTHHSSFLQNW
RLNLDYTRVSDQRYFSDFDSAYGSSTDGYADQKFRVAYYQPHYNIAISAKQFQIFNEVDIGPYRALPQVDFNYYQNALFD
SPLNFKLFSQVVHFDNKSPLMPKAWRFHAEPSINLPLSNRYGSLNIETKLYATHYQQTQGRAAEAEQVERKINRVLPQVK
VDLQTVLASQQTFIEGYTQTLEPHVQYLYRPYKDQSNIGSKLNNAYLGFGYDSSLLQQDYFGLFRDRRYSGLDRIASANQ
FTVGGTTRIYDKKGNERFNFSAGQIYYLQDSRIDNSKENSTYGRSSSWSLASNWKINDQWRWQGSYQYDTRLNKSSLGNF
TLEYNPTGNNIIQLSYRYASQQYIDQNLTSAANRYGQDIKQLGLTVAWALTDQWAVVARHYQDLALRKPVEQYLGLEYST
CCWAINVGARRSVTSKENQTANQIFYDKSIGINIELRGLGRDDHKGNIEKMLQRGKLPYLQAFSLY

Sequences:

>Translated_786_residues
MVYPMKKNSYTRLSIAILSTLYSVSSLADLKSQCLAGVPHFTGELITGNPNDLPVYIEADQARLNQSVSASYEGNVEIQQ
GNRQLRAQRADISQQKQPNGLQRLAHVRGGFEYQDHQIQLKGEEAQVQLNTKNTDIKNADYQLVDRQGRGKAESIALRED
YRLMTNAVFTSCLPQDNAWSIEAKEMRQHIQEEYAEMWHARFKVHGVPIFYTPYLQLPIGDRRRSGLLLPNFGTSNRDGY
FYEQPFYWNIAPNLDATMTPKYMSKRGWQLNGEFRYLSPIGEGKIAGEYLKQDRLTDYRNRDRSRHLFYWTHHSSFLQNW
RLNLDYTRVSDQRYFSDFDSAYGSSTDGYADQKFRVAYYQPHYNIAISAKQFQIFNEVDIGPYRALPQVDFNYYQNALFD
SPLNFKLFSQVVHFDNKSPLMPKAWRFHAEPSINLPLSNRYGSLNIETKLYATHYQQTQGRAAEAEQVERKINRVLPQVK
VDLQTVLASQQTFIEGYTQTLEPHVQYLYRPYKDQSNIGSKLNNAYLGFGYDSSLLQQDYFGLFRDRRYSGLDRIASANQ
FTVGGTTRIYDKKGNERFNFSAGQIYYLQDSRIDNSKENSTYGRSSSWSLASNWKINDQWRWQGSYQYDTRLNKSSLGNF
TLEYNPTGNNIIQLSYRYASQQYIDQNLTSAANRYGQDIKQLGLTVAWALTDQWAVVARHYQDLALRKPVEQYLGLEYST
CCWAINVGARRSVTSKENQTANQIFYDKSIGINIELRGLGRDDHKGNIEKMLQRGKLPYLQAFSLY
>Mature_786_residues
MVYPMKKNSYTRLSIAILSTLYSVSSLADLKSQCLAGVPHFTGELITGNPNDLPVYIEADQARLNQSVSASYEGNVEIQQ
GNRQLRAQRADISQQKQPNGLQRLAHVRGGFEYQDHQIQLKGEEAQVQLNTKNTDIKNADYQLVDRQGRGKAESIALRED
YRLMTNAVFTSCLPQDNAWSIEAKEMRQHIQEEYAEMWHARFKVHGVPIFYTPYLQLPIGDRRRSGLLLPNFGTSNRDGY
FYEQPFYWNIAPNLDATMTPKYMSKRGWQLNGEFRYLSPIGEGKIAGEYLKQDRLTDYRNRDRSRHLFYWTHHSSFLQNW
RLNLDYTRVSDQRYFSDFDSAYGSSTDGYADQKFRVAYYQPHYNIAISAKQFQIFNEVDIGPYRALPQVDFNYYQNALFD
SPLNFKLFSQVVHFDNKSPLMPKAWRFHAEPSINLPLSNRYGSLNIETKLYATHYQQTQGRAAEAEQVERKINRVLPQVK
VDLQTVLASQQTFIEGYTQTLEPHVQYLYRPYKDQSNIGSKLNNAYLGFGYDSSLLQQDYFGLFRDRRYSGLDRIASANQ
FTVGGTTRIYDKKGNERFNFSAGQIYYLQDSRIDNSKENSTYGRSSSWSLASNWKINDQWRWQGSYQYDTRLNKSSLGNF
TLEYNPTGNNIIQLSYRYASQQYIDQNLTSAANRYGQDIKQLGLTVAWALTDQWAVVARHYQDLALRKPVEQYLGLEYST
CCWAINVGARRSVTSKENQTANQIFYDKSIGINIELRGLGRDDHKGNIEKMLQRGKLPYLQAFSLY

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=796, Percent_Identity=45.8542713567839, Blast_Score=679, Evalue=0.0,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 91139; Mature: 91139

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYPMKKNSYTRLSIAILSTLYSVSSLADLKSQCLAGVPHFTGELITGNPNDLPVYIEAD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECC
QARLNQSVSASYEGNVEIQQGNRQLRAQRADISQQKQPNGLQRLAHVRGGFEYQDHQIQL
HHHCCCCCCCCCCCCEEEECCCHHHHHHHHCCHHHCCCCHHHHHHHHHCCCCCCCCEEEE
KGEEAQVQLNTKNTDIKNADYQLVDRQGRGKAESIALREDYRLMTNAVFTSCLPQDNAWS
ECCEEEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCE
IEAKEMRQHIQEEYAEMWHARFKVHGVPIFYTPYLQLPIGDRRRSGLLLPNFGTSNRDGY
EEHHHHHHHHHHHHHHHHHHHEEEECEEEEECCEEEECCCCCCCCCEEECCCCCCCCCCE
FYEQPFYWNIAPNLDATMTPKYMSKRGWQLNGEFRYLSPIGEGKIAGEYLKQDRLTDYRN
EECCCEEEEECCCCCCCCCCHHHHCCCCEECCCEEEECCCCCCCHHHHHHHHHHHHHHHC
RDRSRHLFYWTHHSSFLQNWRLNLDYTRVSDQRYFSDFDSAYGSSTDGYADQKFRVAYYQ
CCCCCEEEEEECCHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEC
PHYNIAISAKQFQIFNEVDIGPYRALPQVDFNYYQNALFDSPLNFKLFSQVVHFDNKSPL
CCEEEEEEEHEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
MPKAWRFHAEPSINLPLSNRYGSLNIETKLYATHYQQTQGRAAEAEQVERKINRVLPQVK
CCCCEEEECCCCCCCCCCCCCCCEEEEEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHH
VDLQTVLASQQTFIEGYTQTLEPHVQYLYRPYKDQSNIGSKLNNAYLGFGYDSSLLQQDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEECCCCHHHHHHHH
FGLFRDRRYSGLDRIASANQFTVGGTTRIYDKKGNERFNFSAGQIYYLQDSRIDNSKENS
HHHHHHHHHCCHHHHCCCCEEEECCEEEEEECCCCCEEEECCCEEEEEECCCCCCCCCCC
TYGRSSSWSLASNWKINDQWRWQGSYQYDTRLNKSSLGNFTLEYNPTGNNIIQLSYRYAS
CCCCCCCCCEECCCEECCEEEECCCEEECCCCCCCCCCCEEEEEECCCCCEEEEEEEHHH
QQYIDQNLTSAANRYGQDIKQLGLTVAWALTDQWAVVARHYQDLALRKPVEQYLGLEYST
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCHHHHHHCCCCCC
CCWAINVGARRSVTSKENQTANQIFYDKSIGINIELRGLGRDDHKGNIEKMLQRGKLPYL
EEEEEECCCCCCCCCCCCCCHHHEEEECCCCEEEEEEECCCCCCCCCHHHHHHHCCCCCH
QAFSLY
HHCCCC
>Mature Secondary Structure
MVYPMKKNSYTRLSIAILSTLYSVSSLADLKSQCLAGVPHFTGELITGNPNDLPVYIEAD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECC
QARLNQSVSASYEGNVEIQQGNRQLRAQRADISQQKQPNGLQRLAHVRGGFEYQDHQIQL
HHHCCCCCCCCCCCCEEEECCCHHHHHHHHCCHHHCCCCHHHHHHHHHCCCCCCCCEEEE
KGEEAQVQLNTKNTDIKNADYQLVDRQGRGKAESIALREDYRLMTNAVFTSCLPQDNAWS
ECCEEEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCE
IEAKEMRQHIQEEYAEMWHARFKVHGVPIFYTPYLQLPIGDRRRSGLLLPNFGTSNRDGY
EEHHHHHHHHHHHHHHHHHHHEEEECEEEEECCEEEECCCCCCCCCEEECCCCCCCCCCE
FYEQPFYWNIAPNLDATMTPKYMSKRGWQLNGEFRYLSPIGEGKIAGEYLKQDRLTDYRN
EECCCEEEEECCCCCCCCCCHHHHCCCCEECCCEEEECCCCCCCHHHHHHHHHHHHHHHC
RDRSRHLFYWTHHSSFLQNWRLNLDYTRVSDQRYFSDFDSAYGSSTDGYADQKFRVAYYQ
CCCCCEEEEEECCHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEC
PHYNIAISAKQFQIFNEVDIGPYRALPQVDFNYYQNALFDSPLNFKLFSQVVHFDNKSPL
CCEEEEEEEHEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
MPKAWRFHAEPSINLPLSNRYGSLNIETKLYATHYQQTQGRAAEAEQVERKINRVLPQVK
CCCCEEEECCCCCCCCCCCCCCCEEEEEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHH
VDLQTVLASQQTFIEGYTQTLEPHVQYLYRPYKDQSNIGSKLNNAYLGFGYDSSLLQQDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEECCCCHHHHHHHH
FGLFRDRRYSGLDRIASANQFTVGGTTRIYDKKGNERFNFSAGQIYYLQDSRIDNSKENS
HHHHHHHHHCCHHHHCCCCEEEECCEEEEEECCCCCEEEECCCEEEEEECCCCCCCCCCC
TYGRSSSWSLASNWKINDQWRWQGSYQYDTRLNKSSLGNFTLEYNPTGNNIIQLSYRYAS
CCCCCCCCCEECCCEECCEEEECCCEEECCCCCCCCCCCEEEEEECCCCCEEEEEEEHHH
QQYIDQNLTSAANRYGQDIKQLGLTVAWALTDQWAVVARHYQDLALRKPVEQYLGLEYST
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCHHHHHHCCCCCC
CCWAINVGARRSVTSKENQTANQIFYDKSIGINIELRGLGRDDHKGNIEKMLQRGKLPYL
EEEEEECCCCCCCCCCCCCCHHHEEEECCCCEEEEEEECCCCCCCCCHHHHHHHCCCCCH
QAFSLY
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]