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The map label for this gene is asnA

Identifier: 15603439

GI number: 15603439

Start: 1782633

End: 1783625

Strand: Direct

Name: asnA

Synonym: PM1574

Alternate gene names: 15603439

Gene position: 1782633-1783625 (Clockwise)

Preceding gene: 15603432

Following gene: 15603442

Centisome position: 78.97

GC content: 39.88

Gene sequence:

>993_bases
ATGAAGAAATCATTTATTTTACAACAACAAGAAATTAGTTTTGCGAAGAACACGTTCACAGAAAAATTAGCAGAACATTT
GGGTATTGTTGAAGTTCAAGGTCCTATCCTAAGCCAAGTTGGTAATGGTATTCAAGATAATCTTTCAGGTGCAGAGAAAG
CGGTGCAAGTAAATGTGAAACAGATCACAGATGCCACCTTTGAGGTTGTTCACTCTTTAGCCAAATGGAAACGTCATACA
CTTGCGCGCTTTAATTTTGCACAAGGTGAAGGGCTATTTGTGCATATGACGGCACTTCGTCCAGATGAAGATTCTTTAGA
TCAAACCCATTCTGTGTATGTAGATCAATGGGACTGGGAAAAAGTGATTTCAGCAGAGCAACGTAACCTTGCCTATTTGA
AAGAAACTGTTCGTGCTATTTATGCCGCGATTTTAGAAACGGAAGAAGCGGTAAGTAAAAAATTCGGTTTAGCCACCTTT
TTGCCAAAGGATATTCAGTTTGTACACAGTGAAGAATTAGTACAACGTTTTCCGAACATGAATGATAAAGAGCGTGAGAA
TGCGATTTGTAAAGAATATGGCGCGGTGTTCTTAATCGGGATTGGTGGCAAATTGTCTGATGGGAAACCACATGATGTAC
GTGCACCTGATTATGATGACTGGACAACGCCATCAGAAGGTGAATACAAAGGATTAAACGGGGATATTTTGGTTTGGAAC
CCAATTTTAGAACGTGCTTTTGAATTATCTTCCATGGGGATCCGTGTGGATGAAACCGCATTACGTAAACAGCTTGCTTT
AACCAACAATGAAGACCGTTTAAAATTTGATTGGCACCAAGATTTAGTCAATGGTCGTTTACCGTTATCTATCGGTGGTG
GGATTGGGCGCTCACGCTTAGTGATGTTGCTATTACAGAAAAAACACATTGGTGAAGTCCAATCCAGTGTGTGGCCAAAA
TGGGTGATGGAGCAGTTTGATAATATTCTTTAA

Upstream 100 bases:

>100_bases
AAGTATATCGCTTATTTCAAAAAAAGTATTGAGTTATAGGGCTTTTGTATTAAATAATATTCAGTGAATTTGATGTAGTA
TATATTTTAAGGTATCGAAA

Downstream 100 bases:

>100_bases
TTATCGTTATTTGTTTCCCTCTTGAAACAAAAGTGCGGTGGTTTTTTGCAAAATTTCCACCGCACTTTTTTCTTTCTTAT
TCTGAAAGGAAAGTAAAGCA

Product: asparagine synthetase AsnA

Products: NA

Alternate protein names: Asparagine synthetase A

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKKSFILQQQEISFAKNTFTEKLAEHLGIVEVQGPILSQVGNGIQDNLSGAEKAVQVNVKQITDATFEVVHSLAKWKRHT
LARFNFAQGEGLFVHMTALRPDEDSLDQTHSVYVDQWDWEKVISAEQRNLAYLKETVRAIYAAILETEEAVSKKFGLATF
LPKDIQFVHSEELVQRFPNMNDKERENAICKEYGAVFLIGIGGKLSDGKPHDVRAPDYDDWTTPSEGEYKGLNGDILVWN
PILERAFELSSMGIRVDETALRKQLALTNNEDRLKFDWHQDLVNGRLPLSIGGGIGRSRLVMLLLQKKHIGEVQSSVWPK
WVMEQFDNIL

Sequences:

>Translated_330_residues
MKKSFILQQQEISFAKNTFTEKLAEHLGIVEVQGPILSQVGNGIQDNLSGAEKAVQVNVKQITDATFEVVHSLAKWKRHT
LARFNFAQGEGLFVHMTALRPDEDSLDQTHSVYVDQWDWEKVISAEQRNLAYLKETVRAIYAAILETEEAVSKKFGLATF
LPKDIQFVHSEELVQRFPNMNDKERENAICKEYGAVFLIGIGGKLSDGKPHDVRAPDYDDWTTPSEGEYKGLNGDILVWN
PILERAFELSSMGIRVDETALRKQLALTNNEDRLKFDWHQDLVNGRLPLSIGGGIGRSRLVMLLLQKKHIGEVQSSVWPK
WVMEQFDNIL
>Mature_330_residues
MKKSFILQQQEISFAKNTFTEKLAEHLGIVEVQGPILSQVGNGIQDNLSGAEKAVQVNVKQITDATFEVVHSLAKWKRHT
LARFNFAQGEGLFVHMTALRPDEDSLDQTHSVYVDQWDWEKVISAEQRNLAYLKETVRAIYAAILETEEAVSKKFGLATF
LPKDIQFVHSEELVQRFPNMNDKERENAICKEYGAVFLIGIGGKLSDGKPHDVRAPDYDDWTTPSEGEYKGLNGDILVWN
PILERAFELSSMGIRVDETALRKQLALTNNEDRLKFDWHQDLVNGRLPLSIGGGIGRSRLVMLLLQKKHIGEVQSSVWPK
WVMEQFDNIL

Specific function: Asparagine biosynthesis. [C]

COG id: COG2502

COG function: function code E; Asparagine synthetase A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily

Homologues:

Organism=Escherichia coli, GI1790183, Length=330, Percent_Identity=63.9393939393939, Blast_Score=444, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASNA_PASMU (Q9CKN6)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246513.1
- ProteinModelPortal:   Q9CKN6
- SMR:   Q9CKN6
- GeneID:   1244921
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1574
- NMPDR:   fig|272843.1.peg.1575
- HOGENOM:   HBG288146
- OMA:   LNDNLNG
- ProtClustDB:   PRK05425
- BioCyc:   PMUL272843:PM1574-MONOMER
- BRENDA:   6.3.1.1
- GO:   GO:0005737
- HAMAP:   MF_00555
- InterPro:   IPR006195
- InterPro:   IPR004618
- PIRSF:   PIRSF001555
- TIGRFAMs:   TIGR00669

Pfam domain/function: PF03590 AsnA

EC number: =6.3.1.1

Molecular weight: Translated: 37463; Mature: 37463

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSFILQQQEISFAKNTFTEKLAEHLGIVEVQGPILSQVGNGIQDNLSGAEKAVQVNVK
CCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHCCCHHHHHHHHHHHHEEHHH
QITDATFEVVHSLAKWKRHTLARFNFAQGEGLFVHMTALRPDEDSLDQTHSVYVDQWDWE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHCCHHEEEECCCCHH
KVISAEQRNLAYLKETVRAIYAAILETEEAVSKKFGLATFLPKDIQFVHSEELVQRFPNM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHCCCC
NDKERENAICKEYGAVFLIGIGGKLSDGKPHDVRAPDYDDWTTPSEGEYKGLNGDILVWN
CCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEC
PILERAFELSSMGIRVDETALRKQLALTNNEDRLKFDWHQDLVNGRLPLSIGGGIGRSRL
HHHHHHHHHHHCCCEECHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCCEEECCCCCHHHH
VMLLLQKKHIGEVQSSVWPKWVMEQFDNIL
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MKKSFILQQQEISFAKNTFTEKLAEHLGIVEVQGPILSQVGNGIQDNLSGAEKAVQVNVK
CCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHCCCHHHHHHHHHHHHEEHHH
QITDATFEVVHSLAKWKRHTLARFNFAQGEGLFVHMTALRPDEDSLDQTHSVYVDQWDWE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHCCHHEEEECCCCHH
KVISAEQRNLAYLKETVRAIYAAILETEEAVSKKFGLATFLPKDIQFVHSEELVQRFPNM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHCCCC
NDKERENAICKEYGAVFLIGIGGKLSDGKPHDVRAPDYDDWTTPSEGEYKGLNGDILVWN
CCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEC
PILERAFELSSMGIRVDETALRKQLALTNNEDRLKFDWHQDLVNGRLPLSIGGGIGRSRL
HHHHHHHHHHHCCCEECHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCCEEECCCCCHHHH
VMLLLQKKHIGEVQSSVWPKWVMEQFDNIL
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100