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The map label for this gene is bcr_2 [H]

Identifier: 15603398

GI number: 15603398

Start: 1731653

End: 1732852

Strand: Direct

Name: bcr_2 [H]

Synonym: PM1533

Alternate gene names: 15603398

Gene position: 1731653-1732852 (Clockwise)

Preceding gene: 15603397

Following gene: 15603399

Centisome position: 76.71

GC content: 44.42

Gene sequence:

>1200_bases
ATGCCAGTAGCAACCAATACGACGTGGAATCAGACCGCACTTATCTGCTTCTTAGGCACATTGGTGGGCTTTGGACCGCT
TTCCATTGATATGTATCTGCCGTCTTTACCGACCATTGCGAAAGCTTTACATACCAGTATTGAATGGGTACAACTGTCCG
TTAGTACTTTTCTGACGGGGTTTTGTGTGGGGATGTTGTTTTATGGTCCCTTATCCGATAAATATGGGCGCCGTATTATT
TTGCTGATTGGCATGACGATTTATATTGTGGCAAGCATTGCGTGCAGTATTGCTACTAGCATTGAACAGCTGTTAGTCGC
CCGTTTTTTACAAGCCTTTGGCGGTGGGGTAGGTCCCGTATTAGGGCGGGCGATTGTGCGTGATAGTTTCCCGCCACAGC
GTATTACGCACGTGCTTTCTATGATGCAGTTAGTCACGATGCTTGCCCCTCTCTTAGCGCCTTTTATCGGCGGTATTGTG
TTGTTATGGTTTGGCTGGGAAACTCAGTTCTTACTTTTAGCGTTAATTGGGGTGGTTTGTTGGTTAATTGCTTATTTTTT
CTTAGCGGAAACCAATCAAGACCGCCATCAACGTCCGTTAAATCTTGGCACATTTTTTCAAGCATATTGTGGGATTTTGA
AACAACCACAATCTTTCGGCAATATTCTTTGTTTAAGTGCCATGTTTGGCGGCATGTTTGCTTATGTAGCCGGTACGCCC
TTTGTTTACATTGATTATTTCCATATTCCGCCTCAGGACTACGGATTTTACTTTGGCATCAATGTGGTTGGTATTGTGTT
AGCAACTTTAATTAATAACTATTGTGTTAAACGTTATGCCGTCCAGCGTGTGTTAATGGCGGAAATGGGCTTAGTGGCAT
TGGCTGGCATGGCGTTTTTTCTGGCAGACCCCGATTCCTTATTTGCCATAATGTTACCGCTTTTTATTTTTGTCGGGTTA
ACGGGGGCGCTCACGCCTAATGTGATGACCAGTTTATTAAAACAGCATAGCCATGCAGCAGGTGCGGCGATGGCACTTGT
GGCTTCGATGCAGTTTGCCGGTGGTTTTGTGGCAAGTGGCGCATTGTCTTACTTTTTTAACAACAGTCCGCAAACCATGC
TTTGGGTCATGAGTACTTGCGCAGGTGTTGCCATTGTGGGCTTTATGATTACGTTAAAACCAAATTCAATTTCATCTTAA

Upstream 100 bases:

>100_bases
ATTGCATCCGACCTTAAATTTCAAGAAAAACAATGTGCATTTGAGATTTAAGAAATAAGCCAATTGAGGCGATTTAACAG
AGTGTCATGGAGGCGCCATT

Downstream 100 bases:

>100_bases
AGAGGGATTTTCTATGTTATCCGAAGCGATTATTCAACAGGCGGCACAACGTCTTAATGAAGCAGAAAAAACAGGCACGC
AGATTTCACAATTTTCGTTA

Product: Bcr

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MPVATNTTWNQTALICFLGTLVGFGPLSIDMYLPSLPTIAKALHTSIEWVQLSVSTFLTGFCVGMLFYGPLSDKYGRRII
LLIGMTIYIVASIACSIATSIEQLLVARFLQAFGGGVGPVLGRAIVRDSFPPQRITHVLSMMQLVTMLAPLLAPFIGGIV
LLWFGWETQFLLLALIGVVCWLIAYFFLAETNQDRHQRPLNLGTFFQAYCGILKQPQSFGNILCLSAMFGGMFAYVAGTP
FVYIDYFHIPPQDYGFYFGINVVGIVLATLINNYCVKRYAVQRVLMAEMGLVALAGMAFFLADPDSLFAIMLPLFIFVGL
TGALTPNVMTSLLKQHSHAAGAAMALVASMQFAGGFVASGALSYFFNNSPQTMLWVMSTCAGVAIVGFMITLKPNSISS

Sequences:

>Translated_399_residues
MPVATNTTWNQTALICFLGTLVGFGPLSIDMYLPSLPTIAKALHTSIEWVQLSVSTFLTGFCVGMLFYGPLSDKYGRRII
LLIGMTIYIVASIACSIATSIEQLLVARFLQAFGGGVGPVLGRAIVRDSFPPQRITHVLSMMQLVTMLAPLLAPFIGGIV
LLWFGWETQFLLLALIGVVCWLIAYFFLAETNQDRHQRPLNLGTFFQAYCGILKQPQSFGNILCLSAMFGGMFAYVAGTP
FVYIDYFHIPPQDYGFYFGINVVGIVLATLINNYCVKRYAVQRVLMAEMGLVALAGMAFFLADPDSLFAIMLPLFIFVGL
TGALTPNVMTSLLKQHSHAAGAAMALVASMQFAGGFVASGALSYFFNNSPQTMLWVMSTCAGVAIVGFMITLKPNSISS
>Mature_398_residues
PVATNTTWNQTALICFLGTLVGFGPLSIDMYLPSLPTIAKALHTSIEWVQLSVSTFLTGFCVGMLFYGPLSDKYGRRIIL
LIGMTIYIVASIACSIATSIEQLLVARFLQAFGGGVGPVLGRAIVRDSFPPQRITHVLSMMQLVTMLAPLLAPFIGGIVL
LWFGWETQFLLLALIGVVCWLIAYFFLAETNQDRHQRPLNLGTFFQAYCGILKQPQSFGNILCLSAMFGGMFAYVAGTPF
VYIDYFHIPPQDYGFYFGINVVGIVLATLINNYCVKRYAVQRVLMAEMGLVALAGMAFFLADPDSLFAIMLPLFIFVGLT
GALTPNVMTSLLKQHSHAAGAAMALVASMQFAGGFVASGALSYFFNNSPQTMLWVMSTCAGVAIVGFMITLKPNSISS

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=402, Percent_Identity=30.3482587064677, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI48994889, Length=362, Percent_Identity=30.6629834254144, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1790146, Length=201, Percent_Identity=34.8258706467662, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1787065, Length=253, Percent_Identity=27.6679841897233, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87082312, Length=358, Percent_Identity=26.536312849162, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790794, Length=253, Percent_Identity=22.1343873517787, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790195, Length=163, Percent_Identity=25.7668711656442, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87081983, Length=165, Percent_Identity=25.4545454545455, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325414, Length=152, Percent_Identity=29.6052631578947, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6319657, Length=196, Percent_Identity=27.0408163265306, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6321577, Length=152, Percent_Identity=28.9473684210526, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319480, Length=269, Percent_Identity=22.3048327137546, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321838, Length=227, Percent_Identity=26.8722466960352, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43352; Mature: 43221

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVATNTTWNQTALICFLGTLVGFGPLSIDMYLPSLPTIAKALHTSIEWVQLSVSTFLTG
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
FCVGMLFYGPLSDKYGRRIILLIGMTIYIVASIACSIATSIEQLLVARFLQAFGGGVGPV
HHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LGRAIVRDSFPPQRITHVLSMMQLVTMLAPLLAPFIGGIVLLWFGWETQFLLLALIGVVC
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
WLIAYFFLAETNQDRHQRPLNLGTFFQAYCGILKQPQSFGNILCLSAMFGGMFAYVAGTP
HHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
FVYIDYFHIPPQDYGFYFGINVVGIVLATLINNYCVKRYAVQRVLMAEMGLVALAGMAFF
EEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADPDSLFAIMLPLFIFVGLTGALTPNVMTSLLKQHSHAAGAAMALVASMQFAGGFVASG
HCCCHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHH
ALSYFFNNSPQTMLWVMSTCAGVAIVGFMITLKPNSISS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCC
>Mature Secondary Structure 
PVATNTTWNQTALICFLGTLVGFGPLSIDMYLPSLPTIAKALHTSIEWVQLSVSTFLTG
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
FCVGMLFYGPLSDKYGRRIILLIGMTIYIVASIACSIATSIEQLLVARFLQAFGGGVGPV
HHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LGRAIVRDSFPPQRITHVLSMMQLVTMLAPLLAPFIGGIVLLWFGWETQFLLLALIGVVC
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
WLIAYFFLAETNQDRHQRPLNLGTFFQAYCGILKQPQSFGNILCLSAMFGGMFAYVAGTP
HHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
FVYIDYFHIPPQDYGFYFGINVVGIVLATLINNYCVKRYAVQRVLMAEMGLVALAGMAFF
EEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADPDSLFAIMLPLFIFVGLTGALTPNVMTSLLKQHSHAAGAAMALVASMQFAGGFVASG
HCCCHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHH
ALSYFFNNSPQTMLWVMSTCAGVAIVGFMITLKPNSISS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]