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The map label for this gene is hpaF [H]

Identifier: 15603397

GI number: 15603397

Start: 1731227

End: 1731610

Strand: Direct

Name: hpaF [H]

Synonym: PM1532

Alternate gene names: 15603397

Gene position: 1731227-1731610 (Clockwise)

Preceding gene: 15603396

Following gene: 15603398

Centisome position: 76.69

GC content: 41.41

Gene sequence:

>384_bases
ATGCCACACTTTATTGTTGAAGCCACCGAAAATTTGCGTGATGACGTGGATTGGCAACCATTATTTAAACAGGTGCACGA
TTATCTTGCCAGTACCGGTATTTTCCCTTTAGGCGGCATTCGTAGCCGTGTTCATTGGGTGGATGTGTATCGTATGGCGG
ATGGGATAGAAGATGATGCTTTTGTACATATCACGTTAAAGATTGGTGCGGGACGTGATGAAGCCAGTTTAAAACCTGTC
GGCGAACAGGTGTTTACACTGTTAACGAATTACTTTCAACCGCTATTTGAGAAACGCTATTTTGCGCTGTCATTTGAAAT
GGCGGAATTGCATCCGACCTTAAATTTCAAGAAAAACAATGTGCATTTGAGATTTAAGAAATAA

Upstream 100 bases:

>100_bases
TAACGCCGTCTTCCCGGTGTAATCCGCACAAGTGCGGTTGGTTTCTGACACTTTTTGCTAAACAGACCGCACTTTTCTCT
TCATAAAAAGGAAAATATGC

Downstream 100 bases:

>100_bases
GCCAATTGAGGCGATTTAACAGAGTGTCATGGAGGCGCCATTATGCCAGTAGCAACCAATACGACGTGGAATCAGACCGC
ACTTATCTGCTTCTTAGGCA

Product: HpaF

Products: NA

Alternate protein names: 5-carboxymethyl-2-hydroxymuconic acid isomerase; CHM isomerase; CHMI [H]

Number of amino acids: Translated: 127; Mature: 126

Protein sequence:

>127_residues
MPHFIVEATENLRDDVDWQPLFKQVHDYLASTGIFPLGGIRSRVHWVDVYRMADGIEDDAFVHITLKIGAGRDEASLKPV
GEQVFTLLTNYFQPLFEKRYFALSFEMAELHPTLNFKKNNVHLRFKK

Sequences:

>Translated_127_residues
MPHFIVEATENLRDDVDWQPLFKQVHDYLASTGIFPLGGIRSRVHWVDVYRMADGIEDDAFVHITLKIGAGRDEASLKPV
GEQVFTLLTNYFQPLFEKRYFALSFEMAELHPTLNFKKNNVHLRFKK
>Mature_126_residues
PHFIVEATENLRDDVDWQPLFKQVHDYLASTGIFPLGGIRSRVHWVDVYRMADGIEDDAFVHITLKIGAGRDEASLKPVG
EQVFTLLTNYFQPLFEKRYFALSFEMAELHPTLNFKKNNVHLRFKK

Specific function: Transforms 5-carboxymethyl-2-hydroxy-muconic acid (CHM) into 5-oxo-pent-3-ene-1,2,5-tricarboxylic acid (OPET) [H]

COG id: COG3232

COG function: function code E; 5-carboxymethyl-2-hydroxymuconate isomerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004220
- InterPro:   IPR014347 [H]

Pfam domain/function: PF02962 CHMI [H]

EC number: =5.3.3.10 [H]

Molecular weight: Translated: 14796; Mature: 14665

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHFIVEATENLRDDVDWQPLFKQVHDYLASTGIFPLGGIRSRVHWVDVYRMADGIEDDA
CCCEEEECCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
FVHITLKIGAGRDEASLKPVGEQVFTLLTNYFQPLFEKRYFALSFEMAELHPTLNFKKNN
EEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHEEEEEHHHCCCCCCEECCC
VHLRFKK
EEEEEEC
>Mature Secondary Structure 
PHFIVEATENLRDDVDWQPLFKQVHDYLASTGIFPLGGIRSRVHWVDVYRMADGIEDDA
CCEEEECCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
FVHITLKIGAGRDEASLKPVGEQVFTLLTNYFQPLFEKRYFALSFEMAELHPTLNFKKNN
EEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHEEEEEHHHCCCCCCEECCC
VHLRFKK
EEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2194841; 2261999; 8547259 [H]