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The map label for this gene is aroE [C]

Identifier: 15603294

GI number: 15603294

Start: 1615496

End: 1616308

Strand: Reverse

Name: aroE [C]

Synonym: PM1429

Alternate gene names: 15603294

Gene position: 1616308-1615496 (Counterclockwise)

Preceding gene: 15603295

Following gene: 15603293

Centisome position: 71.6

GC content: 43.3

Gene sequence:

>813_bases
ATGATTAATAAAGAAACGCAGCTTTGTATTTCACTGGCGGGTAAACCGGGTAATTTTGGCACGGCGTTCCATAATTACTT
ATATCAAAAGCTAGGGCTAAACTATATTTATAAAGCGTTCACCACCTCCGATATTGAACATGCGGTAAAAGGTGTTCGTG
CGCTCGGCATTCGGGGCTGTGCGGTATCCATGCCCTTTAAAGAAAGCTGTATGCCGTTTCTCGATGAAATATCGGCATCA
GCTAAAGCCATTGAGTCGGTGAATACGATAGTGAATGAAGATGGCTATTTAAAAGCCTATAACACCGATTACATTGCGAT
CAGCAAATTGATTCAACAGTATCAACTTGATAAACATCACAAGGTGATTGTGCAGGGGAGCGGTGGCATGGCGAAAGCCG
TTGTTGCAGCATTTAAAAATGCGGGCTTTCACGATTTGAGTGTGTATGCACGTAATGCAACCACAGGACAGTATTTAGCG
AATCTATATGGATATCAATATATCAATCATTTAGAAGGCAATGAAGCATCGATTTTAGTGAATGCAACCCCGATGGGGAT
GTCTGGTGGAGAGGAAAATACGCTCGCTTTCCCTGAAAGTCTGATTGAGCAAGCCAGCAGCATATTTGATGTTGTTGCTT
TGCCCGCAGAAACACCATTAATTCAATATGCGAAACAGCAAAATAAGCAGACCATTTCAGGTGCGGAAGTGATTGTCTTG
CAAGCGGTCGAACAATTCGAACTCTACACGCATCAACGACCAAGCGAAACTTTGATTGCCGAAGCCGCTGAGTTTGCGCG
TTCAATCCGTTAA

Upstream 100 bases:

>100_bases
ACGATCATGCCTCTAAGGTGTAATTTAGCAGTGAGTTAAAAAGTGCGGTCATTTTCCGCACTTTTTTCTTACTGAAGACA
CGTGAGCACAAGGATATTTT

Downstream 100 bases:

>100_bases
TTTTTACATTCTGATTCAATAGGGCACAGTATTGTGCCTTTTTTGTGCTTTTTAGCGAATAAAAAAGCAATCTGATTATC
TTCCCTTTCCTTTCTTGTTT

Product: shikimate 5-dehydrogenase

Products: 3-dehydroshikimate; NADPH; H+

Alternate protein names: SDH-L [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MINKETQLCISLAGKPGNFGTAFHNYLYQKLGLNYIYKAFTTSDIEHAVKGVRALGIRGCAVSMPFKESCMPFLDEISAS
AKAIESVNTIVNEDGYLKAYNTDYIAISKLIQQYQLDKHHKVIVQGSGGMAKAVVAAFKNAGFHDLSVYARNATTGQYLA
NLYGYQYINHLEGNEASILVNATPMGMSGGEENTLAFPESLIEQASSIFDVVALPAETPLIQYAKQQNKQTISGAEVIVL
QAVEQFELYTHQRPSETLIAEAAEFARSIR

Sequences:

>Translated_270_residues
MINKETQLCISLAGKPGNFGTAFHNYLYQKLGLNYIYKAFTTSDIEHAVKGVRALGIRGCAVSMPFKESCMPFLDEISAS
AKAIESVNTIVNEDGYLKAYNTDYIAISKLIQQYQLDKHHKVIVQGSGGMAKAVVAAFKNAGFHDLSVYARNATTGQYLA
NLYGYQYINHLEGNEASILVNATPMGMSGGEENTLAFPESLIEQASSIFDVVALPAETPLIQYAKQQNKQTISGAEVIVL
QAVEQFELYTHQRPSETLIAEAAEFARSIR
>Mature_270_residues
MINKETQLCISLAGKPGNFGTAFHNYLYQKLGLNYIYKAFTTSDIEHAVKGVRALGIRGCAVSMPFKESCMPFLDEISAS
AKAIESVNTIVNEDGYLKAYNTDYIAISKLIQQYQLDKHHKVIVQGSGGMAKAVVAAFKNAGFHDLSVYARNATTGQYLA
NLYGYQYINHLEGNEASILVNATPMGMSGGEENTLAFPESLIEQASSIFDVVALPAETPLIQYAKQQNKQTISGAEVIVL
QAVEQFELYTHQRPSETLIAEAAEFARSIR

Specific function: The physiological substrate is not known. Has much lower activity towards shikimate than AroE [H]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787983, Length=247, Percent_Identity=30.3643724696356, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789675, Length=237, Percent_Identity=24.8945147679325, Blast_Score=79, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320332, Length=264, Percent_Identity=24.2424242424242, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR006151 [H]

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N [H]

EC number: 1.1.1.25

Molecular weight: Translated: 29592; Mature: 29592

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKETQLCISLAGKPGNFGTAFHNYLYQKLGLNYIYKAFTTSDIEHAVKGVRALGIRGC
CCCCHHEEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCE
AVSMPFKESCMPFLDEISASAKAIESVNTIVNEDGYLKAYNTDYIAISKLIQQYQLDKHH
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCC
KVIVQGSGGMAKAVVAAFKNAGFHDLSVYARNATTGQYLANLYGYQYINHLEGNEASILV
EEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
NATPMGMSGGEENTLAFPESLIEQASSIFDVVALPAETPLIQYAKQQNKQTISGAEVIVL
ECCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHH
QAVEQFELYTHQRPSETLIAEAAEFARSIR
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MINKETQLCISLAGKPGNFGTAFHNYLYQKLGLNYIYKAFTTSDIEHAVKGVRALGIRGC
CCCCHHEEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCE
AVSMPFKESCMPFLDEISASAKAIESVNTIVNEDGYLKAYNTDYIAISKLIQQYQLDKHH
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCC
KVIVQGSGGMAKAVVAAFKNAGFHDLSVYARNATTGQYLANLYGYQYINHLEGNEASILV
EEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
NATPMGMSGGEENTLAFPESLIEQASSIFDVVALPAETPLIQYAKQQNKQTISGAEVIVL
ECCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHH
QAVEQFELYTHQRPSETLIAEAAEFARSIR
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: shikimate; NADP+

Specific reaction: shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]