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The map label for this gene is pldA [H]

Identifier: 15603291

GI number: 15603291

Start: 1609960

End: 1610880

Strand: Direct

Name: pldA [H]

Synonym: PM1426

Alternate gene names: 15603291

Gene position: 1609960-1610880 (Clockwise)

Preceding gene: 15603290

Following gene: 15603292

Centisome position: 71.32

GC content: 39.96

Gene sequence:

>921_bases
GTGCCGCTCATGGTGAAATTGTTCACTTTAGTTAAGGAAGTAATCAGAATGTACAAAGCAAAAGGAATTAACCTGTACAA
AAAATGCCTTATCTTAGCAGTGTTAGGCGTCTTTGTTAGCCATGGTACACAGGCAAATGAAAGTGACGCGCGTCCCTATA
AAAGTAAAGCAGAAGTCTTATTAACTAAATCTGATGCCTTCATCGGCTTACTTGGCTACGAAGAAAACTATCTGATGGGG
ACTTATTCAAACAGGCATTTTCTTAAACGCGAAAAAACGCAAAAAGATGAGATTAAGTTCAAAATTAGCTTAGCACTTCC
CCTTTGGCGGGGTATTTTGGGCAATAACTCCGTATTAGCCGCTTCTTATACACAAAAGTCCTGGTTTCAGCTAAGTAATG
TTGATGATTCTTCTCCTTTCCGCGAAACCAATTACGAACCTCAGCTATTTTTGGCTTGGAAAACGCAATATTCCTTACCT
TTTGGCTGGACATTACAGGATGTGGAAACCGGGATAAATCATCAATCCAATGGGCGCGATGATGCGGAAAAACTCTCTCG
CAGTTGGAATCGCCTCTATGTGCGTGCCTCTGCCATAAAACAGAATTGGACAGTTGAAATCAAACCTTGGTGGCGTATTC
CTGAGAAAGCCAAAAATGATGATAACCCAGACATCACTAAATACCGCGGTCATTTTGATGTAGCGTTGGGGTACTACTAT
CACGATCATCAATTTAAACTTTCAGGTCACTATAATCCGATCTCAAACAAAGGGGGATTAGAGGCAAGTTATAGTTACCC
AATCACAAAAAACATTCGTTTCTACACTCAATACTATAATGGCTATGGTGAATCCTTAATTGATTATCAACAACGTATCC
AACGTATTGGTATCGGCATTTCCTTGAACAATGTTTTCTAG

Upstream 100 bases:

>100_bases
TGGTTAGCAAGCCGTGTGGGTGGTTTCCATTTTCGTTATCAAAATAACAATTGGACAAGCAATGATGGAAAAGACTTTTG
GGCGTGTTTAGAAGAAGCTT

Downstream 100 bases:

>100_bases
TTCTCTCCTCTCAAAAGACAATAAAAGTGCGGTGGATTTTCACGAAATTCCGTTAAAACAGACCGCACTTGACGTGTTGC
ATGCTGTTTTTGGTTACCAA

Product: phospholipase A

Products: NA

Alternate protein names: Detergent-resistant phospholipase A; DR-phospholipase A; Outer membrane phospholipase A; OM PLA; OMPLA; Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MPLMVKLFTLVKEVIRMYKAKGINLYKKCLILAVLGVFVSHGTQANESDARPYKSKAEVLLTKSDAFIGLLGYEENYLMG
TYSNRHFLKREKTQKDEIKFKISLALPLWRGILGNNSVLAASYTQKSWFQLSNVDDSSPFRETNYEPQLFLAWKTQYSLP
FGWTLQDVETGINHQSNGRDDAEKLSRSWNRLYVRASAIKQNWTVEIKPWWRIPEKAKNDDNPDITKYRGHFDVALGYYY
HDHQFKLSGHYNPISNKGGLEASYSYPITKNIRFYTQYYNGYGESLIDYQQRIQRIGIGISLNNVF

Sequences:

>Translated_306_residues
MPLMVKLFTLVKEVIRMYKAKGINLYKKCLILAVLGVFVSHGTQANESDARPYKSKAEVLLTKSDAFIGLLGYEENYLMG
TYSNRHFLKREKTQKDEIKFKISLALPLWRGILGNNSVLAASYTQKSWFQLSNVDDSSPFRETNYEPQLFLAWKTQYSLP
FGWTLQDVETGINHQSNGRDDAEKLSRSWNRLYVRASAIKQNWTVEIKPWWRIPEKAKNDDNPDITKYRGHFDVALGYYY
HDHQFKLSGHYNPISNKGGLEASYSYPITKNIRFYTQYYNGYGESLIDYQQRIQRIGIGISLNNVF
>Mature_305_residues
PLMVKLFTLVKEVIRMYKAKGINLYKKCLILAVLGVFVSHGTQANESDARPYKSKAEVLLTKSDAFIGLLGYEENYLMGT
YSNRHFLKREKTQKDEIKFKISLALPLWRGILGNNSVLAASYTQKSWFQLSNVDDSSPFRETNYEPQLFLAWKTQYSLPF
GWTLQDVETGINHQSNGRDDAEKLSRSWNRLYVRASAIKQNWTVEIKPWWRIPEKAKNDDNPDITKYRGHFDVALGYYYH
DHQFKLSGHYNPISNKGGLEASYSYPITKNIRFYTQYYNGYGESLIDYQQRIQRIGIGISLNNVF

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=285, Percent_Identity=49.4736842105263, Blast_Score=259, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 35581; Mature: 35450

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLMVKLFTLVKEVIRMYKAKGINLYKKCLILAVLGVFVSHGTQANESDARPYKSKAEVL
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
LTKSDAFIGLLGYEENYLMGTYSNRHFLKREKTQKDEIKFKISLALPLWRGILGNNSVLA
EEECCCEEEEEECCCCEEEEECCCCHHHHHHCCCCCCEEEEEEEECHHHHHHCCCCCEEE
ASYTQKSWFQLSNVDDSSPFRETNYEPQLFLAWKTQYSLPFGWTLQDVETGINHQSNGRD
EECCCCCCEEECCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHCCCCCCCCCC
DAEKLSRSWNRLYVRASAIKQNWTVEIKPWWRIPEKAKNDDNPDITKYRGHFDVALGYYY
HHHHHHHHHHEEEEEEEHHCCCCEEEEEECCCCCHHCCCCCCCCCEEECCEEEEEEEEEE
HDHQFKLSGHYNPISNKGGLEASYSYPITKNIRFYTQYYNGYGESLIDYQQRIQRIGIGI
ECCEEEECCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCE
SLNNVF
EECCCC
>Mature Secondary Structure 
PLMVKLFTLVKEVIRMYKAKGINLYKKCLILAVLGVFVSHGTQANESDARPYKSKAEVL
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
LTKSDAFIGLLGYEENYLMGTYSNRHFLKREKTQKDEIKFKISLALPLWRGILGNNSVLA
EEECCCEEEEEECCCCEEEEECCCCHHHHHHCCCCCCEEEEEEEECHHHHHHCCCCCEEE
ASYTQKSWFQLSNVDDSSPFRETNYEPQLFLAWKTQYSLPFGWTLQDVETGINHQSNGRD
EECCCCCCEEECCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHCCCCCCCCCC
DAEKLSRSWNRLYVRASAIKQNWTVEIKPWWRIPEKAKNDDNPDITKYRGHFDVALGYYY
HHHHHHHHHHEEEEEEEHHCCCCEEEEEECCCCCHHCCCCCCCCCEEECCEEEEEEEEEE
HDHQFKLSGHYNPISNKGGLEASYSYPITKNIRFYTQYYNGYGESLIDYQQRIQRIGIGI
ECCEEEECCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCE
SLNNVF
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]