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The map label for this gene is trmD

Identifier: 15603162

GI number: 15603162

Start: 1491031

End: 1491768

Strand: Direct

Name: trmD

Synonym: PM1297

Alternate gene names: 15603162

Gene position: 1491031-1491768 (Clockwise)

Preceding gene: 161723309

Following gene: 15603163

Centisome position: 66.05

GC content: 44.85

Gene sequence:

>738_bases
ATGTTAGTTGGGATTATTAGTTTGTTCCCCGAAATGTTCAAAGCCATTACGGAATTTGGGGTCACAGGTAGAGCTGTCAA
ACAGAATCTCTTACAGGTGCGTTGTTGGAATCCCCGTGATTTTACGCACGATAAGCATAAAACAGTGGATGATCGCCCTT
ATGGCGGTGGTCCAGGTATGCTGATGATGGTGCAGCCTTTACGGGATGCGATTCAAGCAGCAAAAGCTGAAGTAGGAGAG
GGGGCGAAAGTGATTTACCTTTCGCCACAAGGACGAAAACTCGATCAAGCAGGCGTAAAAGAACTCGCACAGCATCAGAA
ATTGATTTTACTGTGTGGACGTTACGAAGGCATTGACGAGCGATTAATCGAAACAGAAGTGGATGAAGAATGGTCAGTCG
GTGATTATGTACTGACTGGCGGTGAATTGCCTGCTATGACATTAATTGATGCAGTAGCACGATTTATCCCAGGTGTGCTT
GGTAAACAAGCCTCAGCCGATGAAGATTCGTTTGCTGAAGGCTTGCTTGATTGTCCACATTATACGCGTCCTGAAGTGTT
AGATGGTTTAGCTGTACCACCTGTGCTGATGTCTGGTAATCATGAAGAAATTCGTAAATGGCGATTGAGACAATCGCTTG
AGCGTACATGGTTAAGACGCCCTGAGCTATTAGAAAGCCTAGCTCTGACTGACGAACAACGTAAACTGTTGAAGCAAATT
AAAGCAGAACACAGTTAA

Upstream 100 bases:

>100_bases
ATCTCAACACGAAGACAATTGTCGTGGATTGGGACGCTGGTTTCTAACCTCAGGTATGCCGTTTAGCTTTGGCTGTAGCA
TTTTGATATAAGGGTCACTT

Downstream 100 bases:

>100_bases
TATCAGTTCAATCTAGGATTAGAAAGGTTAATTATTATGAGTAACATCATTAAACAACTTGAACAAGAACAGTTAAAACA
AAACTTACCGAGCTTCCGCC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MLVGIISLFPEMFKAITEFGVTGRAVKQNLLQVRCWNPRDFTHDKHKTVDDRPYGGGPGMLMMVQPLRDAIQAAKAEVGE
GAKVIYLSPQGRKLDQAGVKELAQHQKLILLCGRYEGIDERLIETEVDEEWSVGDYVLTGGELPAMTLIDAVARFIPGVL
GKQASADEDSFAEGLLDCPHYTRPEVLDGLAVPPVLMSGNHEEIRKWRLRQSLERTWLRRPELLESLALTDEQRKLLKQI
KAEHS

Sequences:

>Translated_245_residues
MLVGIISLFPEMFKAITEFGVTGRAVKQNLLQVRCWNPRDFTHDKHKTVDDRPYGGGPGMLMMVQPLRDAIQAAKAEVGE
GAKVIYLSPQGRKLDQAGVKELAQHQKLILLCGRYEGIDERLIETEVDEEWSVGDYVLTGGELPAMTLIDAVARFIPGVL
GKQASADEDSFAEGLLDCPHYTRPEVLDGLAVPPVLMSGNHEEIRKWRLRQSLERTWLRRPELLESLALTDEQRKLLKQI
KAEHS
>Mature_245_residues
MLVGIISLFPEMFKAITEFGVTGRAVKQNLLQVRCWNPRDFTHDKHKTVDDRPYGGGPGMLMMVQPLRDAIQAAKAEVGE
GAKVIYLSPQGRKLDQAGVKELAQHQKLILLCGRYEGIDERLIETEVDEEWSVGDYVLTGGELPAMTLIDAVARFIPGVL
GKQASADEDSFAEGLLDCPHYTRPEVLDGLAVPPVLMSGNHEEIRKWRLRQSLERTWLRRPELLESLALTDEQRKLLKQI
KAEHS

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=245, Percent_Identity=80.4081632653061, Blast_Score=400, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_PASMU (Q9CLE1)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246235.1
- ProteinModelPortal:   Q9CLE1
- SMR:   Q9CLE1
- GeneID:   1244644
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1297
- NMPDR:   fig|272843.1.peg.1298
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   PMUL272843:PM1297-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27495; Mature: 27495

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVGIISLFPEMFKAITEFGVTGRAVKQNLLQVRCWNPRDFTHDKHKTVDDRPYGGGPGM
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCH
LMMVQPLRDAIQAAKAEVGEGAKVIYLSPQGRKLDQAGVKELAQHQKLILLCGRYEGIDE
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHH
RLIETEVDEEWSVGDYVLTGGELPAMTLIDAVARFIPGVLGKQASADEDSFAEGLLDCPH
HHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
YTRPEVLDGLAVPPVLMSGNHEEIRKWRLRQSLERTWLRRPELLESLALTDEQRKLLKQI
CCCHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHH
KAEHS
HHCCC
>Mature Secondary Structure
MLVGIISLFPEMFKAITEFGVTGRAVKQNLLQVRCWNPRDFTHDKHKTVDDRPYGGGPGM
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCH
LMMVQPLRDAIQAAKAEVGEGAKVIYLSPQGRKLDQAGVKELAQHQKLILLCGRYEGIDE
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHH
RLIETEVDEEWSVGDYVLTGGELPAMTLIDAVARFIPGVLGKQASADEDSFAEGLLDCPH
HHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
YTRPEVLDGLAVPPVLMSGNHEEIRKWRLRQSLERTWLRRPELLESLALTDEQRKLLKQI
CCCHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHH
KAEHS
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100