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The map label for this gene is thiE

Identifier: 15603125

GI number: 15603125

Start: 1453439

End: 1454104

Strand: Reverse

Name: thiE

Synonym: PM1260

Alternate gene names: 15603125

Gene position: 1454104-1453439 (Counterclockwise)

Preceding gene: 15603126

Following gene: 15603124

Centisome position: 64.41

GC content: 47.3

Gene sequence:

>666_bases
ATGAAACCAGTCCACGCCTTTATGCGCTTATATTTCATCGCTGGTACGCAAGATTGTTTACATCTTGACGGTGATCCTGC
CCAAAATTTACTCAACATTTTACAACAAGCGCTGCAAAGTGGCATTACTTGCTATCAATTCCGTGAGAAAGGCAAAAAAG
CCCTACAAGACCCCGACAAAATCAAAGCATTAGCCATTCAATGTCGCGACCTCTGCCGTCAATATCAGGTGCCATTTGTG
GTCAATGATGATGTGCAACTAGCCATTGACATTGGCGCAGATGGAATTCATGTGGGGCAGACGGATATGGCGGTGGCGGA
TGTTGCCGCACTTTGCCATAGTCATTGTTTTATTGGCACCTCCGTCAATACCCTTGAACAAGGCATCGCAGCACAAGCCA
ATCCGTTGATTGATTACTTTGGTACAGGTCCTATTTTTCCCACACAATCAAAAGAAGATCCTAAACCGGTGGTCGGTGTG
GATTTTGTGTCAACTATTCGGGCACACGGCATCGACAAACCAATCGTGGCAATTGGCGGTGTGACGACACAGACTGCCGA
GGAATTACGCCGACGTGGCGCGAATGGCGTAGCGGTGATTTCCGCGATCACACAATCCGCCGATATTGCTAAAACTGTAA
AGGAGTTACTGGGTAATGCGCAATAA

Upstream 100 bases:

>100_bases
CGCTGCGATTAGTCATCCGCTCAATATCGGTCATGGTCATGGACCGACAAACCATTGGGCTTATGCTGAATGTAATCAAA
AAAGCCGTTAAGGAGAAAAG

Downstream 100 bases:

>100_bases
TCCACGTAATGTAGCCCTTGCCACAATGGTTGGAACTGCCATTGAGTATTTTGATAATTACATTTATGCCATGGCAGCAG
TCTTGGTGTTTAACCAACAA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MKPVHAFMRLYFIAGTQDCLHLDGDPAQNLLNILQQALQSGITCYQFREKGKKALQDPDKIKALAIQCRDLCRQYQVPFV
VNDDVQLAIDIGADGIHVGQTDMAVADVAALCHSHCFIGTSVNTLEQGIAAQANPLIDYFGTGPIFPTQSKEDPKPVVGV
DFVSTIRAHGIDKPIVAIGGVTTQTAEELRRRGANGVAVISAITQSADIAKTVKELLGNAQ

Sequences:

>Translated_221_residues
MKPVHAFMRLYFIAGTQDCLHLDGDPAQNLLNILQQALQSGITCYQFREKGKKALQDPDKIKALAIQCRDLCRQYQVPFV
VNDDVQLAIDIGADGIHVGQTDMAVADVAALCHSHCFIGTSVNTLEQGIAAQANPLIDYFGTGPIFPTQSKEDPKPVVGV
DFVSTIRAHGIDKPIVAIGGVTTQTAEELRRRGANGVAVISAITQSADIAKTVKELLGNAQ
>Mature_221_residues
MKPVHAFMRLYFIAGTQDCLHLDGDPAQNLLNILQQALQSGITCYQFREKGKKALQDPDKIKALAIQCRDLCRQYQVPFV
VNDDVQLAIDIGADGIHVGQTDMAVADVAALCHSHCFIGTSVNTLEQGIAAQANPLIDYFGTGPIFPTQSKEDPKPVVGV
DFVSTIRAHGIDKPIVAIGGVTTQTAEELRRRGANGVAVISAITQSADIAKTVKELLGNAQ

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=193, Percent_Identity=30.0518134715026, Blast_Score=88, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6325042, Length=211, Percent_Identity=28.436018957346, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_PASMU (P57930)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246197.1
- ProteinModelPortal:   P57930
- GeneID:   1244607
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1260
- NMPDR:   fig|272843.1.peg.1260
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- ProtClustDB:   PRK00043
- BioCyc:   PMUL272843:PM1260-MONOMER
- BRENDA:   2.5.1.3
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23736; Mature: 23736

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPVHAFMRLYFIAGTQDCLHLDGDPAQNLLNILQQALQSGITCYQFREKGKKALQDPDK
CCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHH
IKALAIQCRDLCRQYQVPFVVNDDVQLAIDIGADGIHVGQTDMAVADVAALCHSHCFIGT
HHHHHHHHHHHHHHCCCCEEECCCEEEEEEECCCCEEECCHHHHHHHHHHHHHHHCEECC
SVNTLEQGIAAQANPLIDYFGTGPIFPTQSKEDPKPVVGVDFVSTIRAHGIDKPIVAIGG
CHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCEECHHHHHHHHHCCCCCCEEEECC
VTTQTAEELRRRGANGVAVISAITQSADIAKTVKELLGNAQ
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKPVHAFMRLYFIAGTQDCLHLDGDPAQNLLNILQQALQSGITCYQFREKGKKALQDPDK
CCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHH
IKALAIQCRDLCRQYQVPFVVNDDVQLAIDIGADGIHVGQTDMAVADVAALCHSHCFIGT
HHHHHHHHHHHHHHCCCCEEECCCEEEEEEECCCCEEECCHHHHHHHHHHHHHHHCEECC
SVNTLEQGIAAQANPLIDYFGTGPIFPTQSKEDPKPVVGVDFVSTIRAHGIDKPIVAIGG
CHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCEECHHHHHHHHHCCCCCCEEEECC
VTTQTAEELRRRGANGVAVISAITQSADIAKTVKELLGNAQ
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100