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The map label for this gene is ksgA

Identifier: 15603074

GI number: 15603074

Start: 1401239

End: 1402105

Strand: Direct

Name: ksgA

Synonym: PM1209

Alternate gene names: 15603074

Gene position: 1401239-1402105 (Clockwise)

Preceding gene: 15603073

Following gene: 15603075

Centisome position: 62.07

GC content: 41.64

Gene sequence:

>867_bases
ATGAATTCAAAAAAACATTTAGGTCATACCGCCCGTAAACGTTTTGGGCAAAACTTTTTACATGATAATAATGTCATTCA
TGGCATTGTTTCCGCGATTTATCCCCAAAAAGATCAATTTTTAGTAGAGATCGGTCCCGGTTTAGGGGCATTAACTGAGC
CTGTGGGCGAATTGGTCGATCATTTAACCGTGGTGGAATTAGACCGTGATTTAGCTGAGCGTTTACGCCACCACCCGTTC
TTGCACCATAAAATCACCGTGATTGAAACAGATGCGATGCAGTTTGATTTTGGACAGCTTTATCGTGATGCAAATTTAGC
AGAAAAAAAGCAAAAAATGCGGGTGTTTGGTAACTTACCTTACAACATTTCGACCCCATTAATGTTCCACTTATTTAAAT
ATCACGATTGCATTCAAGACATGCACTTTATGTTGCAAAAAGAAGTGGTAAAACGTTTATGTGCAGGTCCAAACAGCAAA
GCTTATGGGCGCTTAACCATTATGGCGCAATATTTCTGCCAAGTGATGCCGGTGCTTGAAGTACCGCCAACGGCATTTAA
ACCCGCGCCGAAAGTGGATTCTGCTGTTGTACGCTTAGTACCACACAAAGTATTACCGCATCCCGTGAAAGACCTATATT
GGTTAAACCGCGTATGTAGCCAAGCTTTTAATCAACGCCGTAAAACTTTACGCAATGCGTTATCAACTTTATTTAGTGCG
GATCAATTAACGGAATTGGGTATCGATCTCACCGCACGCGCGGAAAACTTATCGATCGCCGATTATGCACGCTTAGCTAA
CTGGTTAACAGACAATCCACCAGCCGATGTTAACAAAGACGAAATGATTGAACATCTAGACGATTAA

Upstream 100 bases:

>100_bases
AATCAATAAAACTTAAAATCCCAGCCCAAATGCTGGGATTTTGCTTTATAATAGACCGCACTTTTGATATCGCATCGTGC
GAACCATTAATCGAGAAAAT

Downstream 100 bases:

>100_bases
TGGCATAAGTGCGGTGTAAAAATGGCAACATATTTAGTAGGCGATTTACAGGGTTGTTACGATGAATTGCAACGTTTACT
GGAAAAAGTGCAGTTCGATT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MNSKKHLGHTARKRFGQNFLHDNNVIHGIVSAIYPQKDQFLVEIGPGLGALTEPVGELVDHLTVVELDRDLAERLRHHPF
LHHKITVIETDAMQFDFGQLYRDANLAEKKQKMRVFGNLPYNISTPLMFHLFKYHDCIQDMHFMLQKEVVKRLCAGPNSK
AYGRLTIMAQYFCQVMPVLEVPPTAFKPAPKVDSAVVRLVPHKVLPHPVKDLYWLNRVCSQAFNQRRKTLRNALSTLFSA
DQLTELGIDLTARAENLSIADYARLANWLTDNPPADVNKDEMIEHLDD

Sequences:

>Translated_288_residues
MNSKKHLGHTARKRFGQNFLHDNNVIHGIVSAIYPQKDQFLVEIGPGLGALTEPVGELVDHLTVVELDRDLAERLRHHPF
LHHKITVIETDAMQFDFGQLYRDANLAEKKQKMRVFGNLPYNISTPLMFHLFKYHDCIQDMHFMLQKEVVKRLCAGPNSK
AYGRLTIMAQYFCQVMPVLEVPPTAFKPAPKVDSAVVRLVPHKVLPHPVKDLYWLNRVCSQAFNQRRKTLRNALSTLFSA
DQLTELGIDLTARAENLSIADYARLANWLTDNPPADVNKDEMIEHLDD
>Mature_288_residues
MNSKKHLGHTARKRFGQNFLHDNNVIHGIVSAIYPQKDQFLVEIGPGLGALTEPVGELVDHLTVVELDRDLAERLRHHPF
LHHKITVIETDAMQFDFGQLYRDANLAEKKQKMRVFGNLPYNISTPLMFHLFKYHDCIQDMHFMLQKEVVKRLCAGPNSK
AYGRLTIMAQYFCQVMPVLEVPPTAFKPAPKVDSAVVRLVPHKVLPHPVKDLYWLNRVCSQAFNQRRKTLRNALSTLFSA
DQLTELGIDLTARAENLSIADYARLANWLTDNPPADVNKDEMIEHLDD

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=284, Percent_Identity=29.9295774647887, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI7657198, Length=257, Percent_Identity=28.4046692607004, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1786236, Length=275, Percent_Identity=67.6363636363636, Blast_Score=388, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25141369, Length=292, Percent_Identity=30.8219178082192, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI25146882, Length=276, Percent_Identity=30.4347826086957, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=249, Percent_Identity=27.3092369477912, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21358017, Length=231, Percent_Identity=32.034632034632, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21357273, Length=289, Percent_Identity=28.719723183391, Blast_Score=95, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_PASMU (Q9CLL5)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246146.1
- ProteinModelPortal:   Q9CLL5
- SMR:   Q9CLL5
- GeneID:   1244556
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1209
- NMPDR:   fig|272843.1.peg.1209
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- ProtClustDB:   PRK00274
- BioCyc:   PMUL272843:PM1209-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 32954; Mature: 32954

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 18-18 BINDING 20-20 BINDING 45-45 BINDING 66-66 BINDING 91-91 BINDING 118-118

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure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HHHHHHCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100