Click here to switch to the map view.

The map label for this gene is vatB [H]

Identifier: 15603062

GI number: 15603062

Start: 1391483

End: 1392106

Strand: Direct

Name: vatB [H]

Synonym: PM1197

Alternate gene names: 15603062

Gene position: 1391483-1392106 (Clockwise)

Preceding gene: 15603061

Following gene: 15603063

Centisome position: 61.64

GC content: 39.1

Gene sequence:

>624_bases
ATGCGATTTCCTGATAAAGATCAGATTTTCCCTATCAAACATTATCACCGACTGTGCTTTTTAAAAAACTTAGTTCGTCA
TCCTAATATTATTGTGGGGGATTTTACCTACTACGATGATGCAGAGAATGTGGAAAATTTTGAGAAAAATGTGAAATACC
ATTTTGACTTTTTAGGCGATAAATTGATCATCGGTAAATTTTGCATGATTGGATCGGATGTGAAATTTATTATGAATGGC
GCCAATCATCAAATGAATGCCATCTCCACTTACCCATTTGGGATTTTTGGTTATGAATGGGCAAAAGCGTTACCGGAGAA
AGCCGAATTATCGAGCAAAGGGGATACGGTTATTGGGAATGAGGTTTGGATTGGCTATAACGCCACGATTATGCCAGGCG
TAAAAATTGGCGATGGCGCGATCATTGGCACAAATTCGGTTGTCACCAAGAATGTGCCACCTTATGCGATTGTCGCAGGA
AATCCCGCTCGCGTGATTAGAAAACGTTTCTCACCTGAAAAAATTGAACAATTATTAGCATTAAAATGGTGGGATTGGGA
GATTGAGAAAATCACGCGTAATCTTGACTTTTTAACTGGCAAAATCAGTGACCCGATTGAGTAA

Upstream 100 bases:

>100_bases
CCCGCAGGCGCGTAAAGCAATAGAAACGGCAGTGAATTGGGCAAAAACACAATGCGATGTAACAACAGAAAAATAACAAC
ACAACAAGGTTAGGTATGCT

Downstream 100 bases:

>100_bases
TCAAGGAAACAATATGCAAACACTTATTTGGAATACGTTAAATTCAGCACAAAAACAAGCTGCACTTTCTCGCCCAGCTC
AAGCCGTTGGTGAACAAATT

Product: VatB

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MRFPDKDQIFPIKHYHRLCFLKNLVRHPNIIVGDFTYYDDAENVENFEKNVKYHFDFLGDKLIIGKFCMIGSDVKFIMNG
ANHQMNAISTYPFGIFGYEWAKALPEKAELSSKGDTVIGNEVWIGYNATIMPGVKIGDGAIIGTNSVVTKNVPPYAIVAG
NPARVIRKRFSPEKIEQLLALKWWDWEIEKITRNLDFLTGKISDPIE

Sequences:

>Translated_207_residues
MRFPDKDQIFPIKHYHRLCFLKNLVRHPNIIVGDFTYYDDAENVENFEKNVKYHFDFLGDKLIIGKFCMIGSDVKFIMNG
ANHQMNAISTYPFGIFGYEWAKALPEKAELSSKGDTVIGNEVWIGYNATIMPGVKIGDGAIIGTNSVVTKNVPPYAIVAG
NPARVIRKRFSPEKIEQLLALKWWDWEIEKITRNLDFLTGKISDPIE
>Mature_207_residues
MRFPDKDQIFPIKHYHRLCFLKNLVRHPNIIVGDFTYYDDAENVENFEKNVKYHFDFLGDKLIIGKFCMIGSDVKFIMNG
ANHQMNAISTYPFGIFGYEWAKALPEKAELSSKGDTVIGNEVWIGYNATIMPGVKIGDGAIIGTNSVVTKNVPPYAIVAG
NPARVIRKRFSPEKIEQLLALKWWDWEIEKITRNLDFLTGKISDPIE

Specific function: Inactivates the A compounds of virginiamycin-like antibiotics, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=69, Percent_Identity=47.8260869565217, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1786664, Length=51, Percent_Identity=56.8627450980392, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1788345, Length=64, Percent_Identity=43.75, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 23646; Mature: 23646

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFPDKDQIFPIKHYHRLCFLKNLVRHPNIIVGDFTYYDDAENVENFEKNVKYHFDFLGD
CCCCCCCCEECHHHHHHHHHHHHHHHCCCEEEECCEECCCHHHHHHHHHCCEEEEECCCC
KLIIGKFCMIGSDVKFIMNGANHQMNAISTYPFGIFGYEWAKALPEKAELSSKGDTVIGN
EEEEHHHHHCCCCCEEEECCCCCCCCEEECCCCCCHHHHHHHHCCCHHHCCCCCCEEECC
EVWIGYNATIMPGVKIGDGAIIGTNSVVTKNVPPYAIVAGNPARVIRKRFSPEKIEQLLA
EEEEECCCEEECCEEECCCEEEECCCEEECCCCCEEEEECCHHHHHHHCCCHHHHHHHHH
LKWWDWEIEKITRNLDFLTGKISDPIE
HHCCCCHHHHHHHHHHHEECCCCCCCC
>Mature Secondary Structure
MRFPDKDQIFPIKHYHRLCFLKNLVRHPNIIVGDFTYYDDAENVENFEKNVKYHFDFLGD
CCCCCCCCEECHHHHHHHHHHHHHHHCCCEEEECCEECCCHHHHHHHHHCCEEEEECCCC
KLIIGKFCMIGSDVKFIMNGANHQMNAISTYPFGIFGYEWAKALPEKAELSSKGDTVIGN
EEEEHHHHHCCCCCEEEECCCCCCCCEEECCCCCCHHHHHHHHCCCHHHCCCCCCEEECC
EVWIGYNATIMPGVKIGDGAIIGTNSVVTKNVPPYAIVAGNPARVIRKRFSPEKIEQLLA
EEEEECCCEEECCEEECCCEEEECCCEEECCCCCEEEEECCHHHHHHHCCCHHHHHHHHH
LKWWDWEIEKITRNLDFLTGKISDPIE
HHCCCCHHHHHHHHHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8344533; 3149758 [H]