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The map label for this gene is ushA [H]

Identifier: 15603058

GI number: 15603058

Start: 1387630

End: 1389279

Strand: Direct

Name: ushA [H]

Synonym: PM1193

Alternate gene names: 15603058

Gene position: 1387630-1389279 (Clockwise)

Preceding gene: 15603057

Following gene: 15603059

Centisome position: 61.47

GC content: 41.09

Gene sequence:

>1650_bases
ATGAAAAAATTAACAAAATTGAGTGCAATCGCCCTGTCATTAGGGTTAATCAGTCAAGCTTACGCTTATACCCAAGACAA
AACCTACAATATTACGGTATTACATGCCAACGACACACACGGTCATTTCTGGAAAAACAGTCACGGCGAGTATGGTTTTG
CTGCACATAAAACTTTAATTGATAACATTCGTAAAGAAGTCGAAGGCAAAGGTGGCTCAGTGATTTTGCTGCATGCAGGT
GATTTCAACACAGGCGTACCTGAATCTGATATGCAAAATGCGAAACCTGATATCGAGGGTATGAACATGATTGGTTATGA
CGTAGCAGTACTCGGTAACCATGAATTCGATTTCCCGATGCAATTGCTTGAAATGCAAGAAAAATGGGCGAAGTTCCCCC
TCATTTCAGCTAACGTCATCAATAAAAAAACCAACAAACCTTTAGTCAAACCTTATGTGATACTAAACAAAGAGGATGTC
AAAATTGCTGTCGTTGGTTTGACCACAGAAGATACCGCTAAATTAGGTAATCCTGATGTGGTTGATCACGTGATCTTTAA
CAACCCAATTGAAACCGCCAAAAACACCTTGGCTGAAATTAACCAAACCGAAAAACCGGATATCCGTATTGCGTTAACCC
ACATGGGCTACTATTTTGATGGTAAACACGGACATAATGCGCCGGGCGATGTCACCATGGCACGTACCCTAGATAAAGGC
GCGTTTGATTTAATTATCGGTGGTCATACACACGACACGGTGTGTATTGATGAACAAGGTCAATTCAAACTGAAATACAC
GCCGGGTGAAGCATGTAAACCTGATTTCCAAAACGGCACTTGGATTGTCCAAGCGGGCGAATGGGGAAAATACATTGGTC
GTGCAGATTTTGAATTCAAAAATGGCGAAACCAAATTGGTGAAATACGAGTTAATTCCAATTAACTTAAAACAAAAAATC
AAATTGGAAGACGGCAAATCAGAATACAAACTGTATCAGTCTGAAATTGCTGAAGATCCAGCCGTCTTTGCCCATTTGAA
GAAATACCAAGACGAAGGCGATCGTTTATTAGGGGTGAAAGTGGGGGAAGTAAAAGGGAAATTTATCGGTGATCGTAAAA
TTATCCGCTTCCACCAAACTAACTTAGGACGTTTAATTGCTCAGTCACAAATGGAGCGTGTGAAAGCGGATGTGGGGATC
ATGAACTCAGGCGGGATTCGTACCGACATCAACGAAGGAGAAACGACATATAAAGATCTCCTTACTGTACAACCTTTCGG
TAATATGATTGCCACTGTCGATTTCACGGGGCAAGAGTTATTAGACTACCTCAATGTGGTGGCATTAAAACAAGTAGATA
GTGGTGCTTACCCACAATTTGCTGGGCTTTCTATGGTAGTTGACCGCACCGCACAAAAAGTATCGGATGTCAAAGTCGGT
GGCAAAGCACTGGATTTAAACAAAACCTATAAAGTATCTGTACCAGACTATTGCGCCGGTGGTGGTGATGGCTATCCTGT
CTTGAAAAAACACCCAAGTTATGTCAATACGGGTTTTATCGATGCGGAGATGCTGAAAAAATACTTTGAAGAAAACAAAG
TGTTAGATGCGTCTAAATACGATCCAAAAGATGACATCATCTTCAAATAA

Upstream 100 bases:

>100_bases
ATTTTTAGGTTAAAGCTCACAAATTTAATGCAAAGTACTTGGCTTGACACAAAATTTTAAGTATATAGTTAGCGGATTTT
TTTCTCTTAAGAGGTTTACC

Downstream 100 bases:

>100_bases
GATGACGAAAAAAAGCGTCTATGATGCCCCGCAATTTTTCGAGTTATATCAAAAATTGCGGGAAAATCCCCTCAGCTTAA
ATGAAGTGGTAGAAAAACCG

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MKKLTKLSAIALSLGLISQAYAYTQDKTYNITVLHANDTHGHFWKNSHGEYGFAAHKTLIDNIRKEVEGKGGSVILLHAG
DFNTGVPESDMQNAKPDIEGMNMIGYDVAVLGNHEFDFPMQLLEMQEKWAKFPLISANVINKKTNKPLVKPYVILNKEDV
KIAVVGLTTEDTAKLGNPDVVDHVIFNNPIETAKNTLAEINQTEKPDIRIALTHMGYYFDGKHGHNAPGDVTMARTLDKG
AFDLIIGGHTHDTVCIDEQGQFKLKYTPGEACKPDFQNGTWIVQAGEWGKYIGRADFEFKNGETKLVKYELIPINLKQKI
KLEDGKSEYKLYQSEIAEDPAVFAHLKKYQDEGDRLLGVKVGEVKGKFIGDRKIIRFHQTNLGRLIAQSQMERVKADVGI
MNSGGIRTDINEGETTYKDLLTVQPFGNMIATVDFTGQELLDYLNVVALKQVDSGAYPQFAGLSMVVDRTAQKVSDVKVG
GKALDLNKTYKVSVPDYCAGGGDGYPVLKKHPSYVNTGFIDAEMLKKYFEENKVLDASKYDPKDDIIFK

Sequences:

>Translated_549_residues
MKKLTKLSAIALSLGLISQAYAYTQDKTYNITVLHANDTHGHFWKNSHGEYGFAAHKTLIDNIRKEVEGKGGSVILLHAG
DFNTGVPESDMQNAKPDIEGMNMIGYDVAVLGNHEFDFPMQLLEMQEKWAKFPLISANVINKKTNKPLVKPYVILNKEDV
KIAVVGLTTEDTAKLGNPDVVDHVIFNNPIETAKNTLAEINQTEKPDIRIALTHMGYYFDGKHGHNAPGDVTMARTLDKG
AFDLIIGGHTHDTVCIDEQGQFKLKYTPGEACKPDFQNGTWIVQAGEWGKYIGRADFEFKNGETKLVKYELIPINLKQKI
KLEDGKSEYKLYQSEIAEDPAVFAHLKKYQDEGDRLLGVKVGEVKGKFIGDRKIIRFHQTNLGRLIAQSQMERVKADVGI
MNSGGIRTDINEGETTYKDLLTVQPFGNMIATVDFTGQELLDYLNVVALKQVDSGAYPQFAGLSMVVDRTAQKVSDVKVG
GKALDLNKTYKVSVPDYCAGGGDGYPVLKKHPSYVNTGFIDAEMLKKYFEENKVLDASKYDPKDDIIFK
>Mature_549_residues
MKKLTKLSAIALSLGLISQAYAYTQDKTYNITVLHANDTHGHFWKNSHGEYGFAAHKTLIDNIRKEVEGKGGSVILLHAG
DFNTGVPESDMQNAKPDIEGMNMIGYDVAVLGNHEFDFPMQLLEMQEKWAKFPLISANVINKKTNKPLVKPYVILNKEDV
KIAVVGLTTEDTAKLGNPDVVDHVIFNNPIETAKNTLAEINQTEKPDIRIALTHMGYYFDGKHGHNAPGDVTMARTLDKG
AFDLIIGGHTHDTVCIDEQGQFKLKYTPGEACKPDFQNGTWIVQAGEWGKYIGRADFEFKNGETKLVKYELIPINLKQKI
KLEDGKSEYKLYQSEIAEDPAVFAHLKKYQDEGDRLLGVKVGEVKGKFIGDRKIIRFHQTNLGRLIAQSQMERVKADVGI
MNSGGIRTDINEGETTYKDLLTVQPFGNMIATVDFTGQELLDYLNVVALKQVDSGAYPQFAGLSMVVDRTAQKVSDVKVG
GKALDLNKTYKVSVPDYCAGGGDGYPVLKKHPSYVNTGFIDAEMLKKYFEENKVLDASKYDPKDDIIFK

Specific function: Degradation of extracellular 5'-nucleotides for nutritional needs [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=571, Percent_Identity=25.569176882662, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1786687, Length=552, Percent_Identity=54.5289855072464, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI1790658, Length=563, Percent_Identity=22.0248667850799, Blast_Score=102, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19922446, Length=524, Percent_Identity=28.0534351145038, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24654424, Length=524, Percent_Identity=28.0534351145038, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI161076508, Length=456, Percent_Identity=26.9736842105263, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI19921980, Length=456, Percent_Identity=26.9736842105263, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24652512, Length=456, Percent_Identity=26.9736842105263, Blast_Score=119, Evalue=8e-27,
Organism=Drosophila melanogaster, GI28573524, Length=561, Percent_Identity=26.2032085561497, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19922444, Length=544, Percent_Identity=25, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24641187, Length=545, Percent_Identity=23.302752293578, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI221329836, Length=541, Percent_Identity=23.4750462107209, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 61096; Mature: 61096

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTKLSAIALSLGLISQAYAYTQDKTYNITVLHANDTHGHFWKNSHGEYGFAAHKTLI
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
DNIRKEVEGKGGSVILLHAGDFNTGVPESDMQNAKPDIEGMNMIGYDVAVLGNHEFDFPM
HHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCCCEEECEEEEEECCCCCCCHH
QLLEMQEKWAKFPLISANVINKKTNKPLVKPYVILNKEDVKIAVVGLTTEDTAKLGNPDV
HHHHHHHHHHCCCEEECHHHCCCCCCCCCCEEEEEECCCEEEEEEEECCCHHHHCCCCCH
VDHVIFNNPIETAKNTLAEINQTEKPDIRIALTHMGYYFDGKHGHNAPGDVTMARTLDKG
HHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCEEECCCCCCCCCCCEEEEEECCCC
AFDLIIGGHTHDTVCIDEQGQFKLKYTPGEACKPDFQNGTWIVQAGEWGKYIGRADFEFK
CEEEEECCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEC
NGETKLVKYELIPINLKQKIKLEDGKSEYKLYQSEIAEDPAVFAHLKKYQDEGDRLLGVK
CCCEEEEEEEEEEECCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEE
VGEVKGKFIGDRKIIRFHQTNLGRLIAQSQMERVKADVGIMNSGGIRTDINEGETTYKDL
EECCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCEECCCCEEECCCCCCCCHHHE
LTVQPFGNMIATVDFTGQELLDYLNVVALKQVDSGAYPQFAGLSMVVDRTAQKVSDVKVG
EEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
GKALDLNKTYKVSVPDYCAGGGDGYPVLKKHPSYVNTGFIDAEMLKKYFEENKVLDASKY
CEEEECCCEEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCEECCCCC
DPKDDIIFK
CCCCCCCCC
>Mature Secondary Structure
MKKLTKLSAIALSLGLISQAYAYTQDKTYNITVLHANDTHGHFWKNSHGEYGFAAHKTLI
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
DNIRKEVEGKGGSVILLHAGDFNTGVPESDMQNAKPDIEGMNMIGYDVAVLGNHEFDFPM
HHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCCCEEECEEEEEECCCCCCCHH
QLLEMQEKWAKFPLISANVINKKTNKPLVKPYVILNKEDVKIAVVGLTTEDTAKLGNPDV
HHHHHHHHHHCCCEEECHHHCCCCCCCCCCEEEEEECCCEEEEEEEECCCHHHHCCCCCH
VDHVIFNNPIETAKNTLAEINQTEKPDIRIALTHMGYYFDGKHGHNAPGDVTMARTLDKG
HHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCEEECCCCCCCCCCCEEEEEECCCC
AFDLIIGGHTHDTVCIDEQGQFKLKYTPGEACKPDFQNGTWIVQAGEWGKYIGRADFEFK
CEEEEECCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEC
NGETKLVKYELIPINLKQKIKLEDGKSEYKLYQSEIAEDPAVFAHLKKYQDEGDRLLGVK
CCCEEEEEEEEEEECCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEE
VGEVKGKFIGDRKIIRFHQTNLGRLIAQSQMERVKADVGIMNSGGIRTDINEGETTYKDL
EECCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCEECCCCEEECCCCCCCCHHHE
LTVQPFGNMIATVDFTGQELLDYLNVVALKQVDSGAYPQFAGLSMVVDRTAQKVSDVKVG
EEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
GKALDLNKTYKVSVPDYCAGGGDGYPVLKKHPSYVNTGFIDAEMLKKYFEENKVLDASKY
CEEEECCCEEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCEECCCCC
DPKDDIIFK
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA