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The map label for this gene is glnA [H]

Identifier: 15603040

GI number: 15603040

Start: 1371025

End: 1372443

Strand: Direct

Name: glnA [H]

Synonym: PM1175

Alternate gene names: 15603040

Gene position: 1371025-1372443 (Clockwise)

Preceding gene: 15603037

Following gene: 15603041

Centisome position: 60.73

GC content: 41.72

Gene sequence:

>1419_bases
ATGTCAGACACCACCGCTATCGCCAACGTATTCAAGCTGATTGAAGAATACGATATCAAATTTGTTTTACTTCGCTTTAC
CGATATTAAGGGGAAAGAACACGGTGTTTCGCTTCCTGTTAATCTTGTTGATGAAGATTTATTTGAAGACGGTAAAATGT
TCGACGGTTCTTCCGTTGAAGGATGGAAGGCAATCAATAAAGCAGATATGCTCTTGATGCCAATGCCAGAAACAGCTGTG
GTTGATCCTTTTGCTCAAATTCCTACCCTTTCCCTCCGTTGCAGTATCTACGAACCTTCTACTATGCAAAGCTACGATCG
TGATCCACGTTCTATTGCGATTCGTGCAGAAAACTATATGCGTTCAACGGGAATAGCCGATGAAGCCCTCTTTGGGCCTG
AACCAGAATTTTTCTTATTTGATGATGTTCGTTTCGATGTCTCGATGAACCGTAGCAGTTATTCTGTTGATGATATTGAG
GCTGCGTGGAATACGAATAAAAAATATGAAGACGGTAACAACGCTTATCGTCCACTCAAAAAAGGCGGTTATTGTGCGGT
CGCCCCGATTGATAGCGCACATGATATCCGCTCTGAAATGTGCTTAATCTTAGAAGAAATGGGCTTAGTGATTGAAGCAC
ACCACCATGAAGTCGCCACAGCAGGACAAAATGAAATTGCAACGCGCTTTAATACCTTAACAACTAAAGCCGACGAAACC
CAAATTTATAAATATGTAGTACAAAACGTCGCCTACGAGCACGGTAAAACTGCCTGCTTTATGCCAAAACCCATCACTGG
CGATAACGGCTCCGGTATGCATTGTAATATGTCATTAAGCAAAGACGGCAAAAACGTTTTCCAAGGGGATAAATACGCTG
GACTGTCTGAAACCGCACTTTATTATATTGGTGGGATTATTAAACATGCAAAAGCGTTAAATGCGTTCACTAATCCTTCA
ACCAACTCTTATAAACGCTTAGTGCCGGGCTTTGAAGCACCTGTCCTATTAGCGTATTCTGCAAGTAACCGTTCAGCCTC
AATTCGCATCCCTGCGGTCACCAATCCAAAAGCGATTCGTATTGAAGCGCGTTTCCCAGATCCATTGGCTAATCCTTATC
TTGCGTTTGCTGCATTATTAATGGCGGGTCTAGACGGGATTATCAATAAAATTCACCCTGGCGATGCGATGGACAAAAAC
CTGTATGATCTGCCACCAGAAGAACTACAAAATATCCCTGCGGTAGCAAGCTCATTAGAAGAAGCTTTAAATGCATTAGA
ACAAGATTATGAATTTTTAACCAAAGGAAATGTGTTTACTCAAGCTTTTATTGATGCGTTTATTACAATTAAGCGGAAAG
AAGTAGAACGTTTAAATATGACCCCACACCCCGTGGAATTCGAAATGTACTATGCCTAA

Upstream 100 bases:

>100_bases
AGGGATATATCCGAAAAACACGAATAAAATACTAGATTTATAGTATAACTTTTATTATATTGAATTCTTTTAAATACGCT
TCTAACACTAAGGATCCTCT

Downstream 100 bases:

>100_bases
CAAGGCAAAGGCGGATTTTTTATTTTAATCTGAATACAAGGTACCCATTGTATATTCTCTGATTATCCCAGTGGGTTTCC
AATTTAATGACAGGAGCGTT

Product: hypothetical protein

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSDTTAIANVFKLIEEYDIKFVLLRFTDIKGKEHGVSLPVNLVDEDLFEDGKMFDGSSVEGWKAINKADMLLMPMPETAV
VDPFAQIPTLSLRCSIYEPSTMQSYDRDPRSIAIRAENYMRSTGIADEALFGPEPEFFLFDDVRFDVSMNRSSYSVDDIE
AAWNTNKKYEDGNNAYRPLKKGGYCAVAPIDSAHDIRSEMCLILEEMGLVIEAHHHEVATAGQNEIATRFNTLTTKADET
QIYKYVVQNVAYEHGKTACFMPKPITGDNGSGMHCNMSLSKDGKNVFQGDKYAGLSETALYYIGGIIKHAKALNAFTNPS
TNSYKRLVPGFEAPVLLAYSASNRSASIRIPAVTNPKAIRIEARFPDPLANPYLAFAALLMAGLDGIINKIHPGDAMDKN
LYDLPPEELQNIPAVASSLEEALNALEQDYEFLTKGNVFTQAFIDAFITIKRKEVERLNMTPHPVEFEMYYA

Sequences:

>Translated_472_residues
MSDTTAIANVFKLIEEYDIKFVLLRFTDIKGKEHGVSLPVNLVDEDLFEDGKMFDGSSVEGWKAINKADMLLMPMPETAV
VDPFAQIPTLSLRCSIYEPSTMQSYDRDPRSIAIRAENYMRSTGIADEALFGPEPEFFLFDDVRFDVSMNRSSYSVDDIE
AAWNTNKKYEDGNNAYRPLKKGGYCAVAPIDSAHDIRSEMCLILEEMGLVIEAHHHEVATAGQNEIATRFNTLTTKADET
QIYKYVVQNVAYEHGKTACFMPKPITGDNGSGMHCNMSLSKDGKNVFQGDKYAGLSETALYYIGGIIKHAKALNAFTNPS
TNSYKRLVPGFEAPVLLAYSASNRSASIRIPAVTNPKAIRIEARFPDPLANPYLAFAALLMAGLDGIINKIHPGDAMDKN
LYDLPPEELQNIPAVASSLEEALNALEQDYEFLTKGNVFTQAFIDAFITIKRKEVERLNMTPHPVEFEMYYA
>Mature_471_residues
SDTTAIANVFKLIEEYDIKFVLLRFTDIKGKEHGVSLPVNLVDEDLFEDGKMFDGSSVEGWKAINKADMLLMPMPETAVV
DPFAQIPTLSLRCSIYEPSTMQSYDRDPRSIAIRAENYMRSTGIADEALFGPEPEFFLFDDVRFDVSMNRSSYSVDDIEA
AWNTNKKYEDGNNAYRPLKKGGYCAVAPIDSAHDIRSEMCLILEEMGLVIEAHHHEVATAGQNEIATRFNTLTTKADETQ
IYKYVVQNVAYEHGKTACFMPKPITGDNGSGMHCNMSLSKDGKNVFQGDKYAGLSETALYYIGGIIKHAKALNAFTNPST
NSYKRLVPGFEAPVLLAYSASNRSASIRIPAVTNPKAIRIEARFPDPLANPYLAFAALLMAGLDGIINKIHPGDAMDKNL
YDLPPEELQNIPAVASSLEEALNALEQDYEFLTKGNVFTQAFIDAFITIKRKEVERLNMTPHPVEFEMYYA

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=258, Percent_Identity=27.906976744186, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1790301, Length=464, Percent_Identity=70.0431034482759, Blast_Score=701, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=175, Percent_Identity=31.4285714285714, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52581; Mature: 52450

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTTAIANVFKLIEEYDIKFVLLRFTDIKGKEHGVSLPVNLVDEDLFEDGKMFDGSSVE
CCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
GWKAINKADMLLMPMPETAVVDPFAQIPTLSLRCSIYEPSTMQSYDRDPRSIAIRAENYM
HHHHCCCCCEEEECCCCHHHCCCHHHCCCEEEEEEEECCCHHHHHCCCCCEEEEEEHHHH
RSTGIADEALFGPEPEFFLFDDVRFDVSMNRSSYSVDDIEAAWNTNKKYEDGNNAYRPLK
HHCCCCHHHCCCCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCHHC
KGGYCAVAPIDSAHDIRSEMCLILEEMGLVIEAHHHEVATAGQNEIATRFNTLTTKADET
CCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCHHHHHHHHHHCCCCCHH
QIYKYVVQNVAYEHGKTACFMPKPITGDNGSGMHCNMSLSKDGKNVFQGDKYAGLSETAL
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCCCCHHHH
YYIGGIIKHAKALNAFTNPSTNSYKRLVPGFEAPVLLAYSASNRSASIRIPAVTNPKAIR
HHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCEEE
IEARFPDPLANPYLAFAALLMAGLDGIINKIHPGDAMDKNLYDLPPEELQNIPAVASSLE
EEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHH
EALNALEQDYEFLTKGNVFTQAFIDAFITIKRKEVERLNMTPHPVEFEMYYA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
>Mature Secondary Structure 
SDTTAIANVFKLIEEYDIKFVLLRFTDIKGKEHGVSLPVNLVDEDLFEDGKMFDGSSVE
CCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
GWKAINKADMLLMPMPETAVVDPFAQIPTLSLRCSIYEPSTMQSYDRDPRSIAIRAENYM
HHHHCCCCCEEEECCCCHHHCCCHHHCCCEEEEEEEECCCHHHHHCCCCCEEEEEEHHHH
RSTGIADEALFGPEPEFFLFDDVRFDVSMNRSSYSVDDIEAAWNTNKKYEDGNNAYRPLK
HHCCCCHHHCCCCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCHHC
KGGYCAVAPIDSAHDIRSEMCLILEEMGLVIEAHHHEVATAGQNEIATRFNTLTTKADET
CCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCHHHHHHHHHHCCCCCHH
QIYKYVVQNVAYEHGKTACFMPKPITGDNGSGMHCNMSLSKDGKNVFQGDKYAGLSETAL
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCCCCHHHH
YYIGGIIKHAKALNAFTNPSTNSYKRLVPGFEAPVLLAYSASNRSASIRIPAVTNPKAIR
HHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCEEE
IEARFPDPLANPYLAFAALLMAGLDGIINKIHPGDAMDKNLYDLPPEELQNIPAVASSLE
EEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHH
EALNALEQDYEFLTKGNVFTQAFIDAFITIKRKEVERLNMTPHPVEFEMYYA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]