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The map label for this gene is glyS

Identifier: 15602967

GI number: 15602967

Start: 1295412

End: 1297481

Strand: Direct

Name: glyS

Synonym: PM1102

Alternate gene names: 15602967

Gene position: 1295412-1297481 (Clockwise)

Preceding gene: 15602966

Following gene: 15602969

Centisome position: 57.38

GC content: 45.65

Gene sequence:

>2070_bases
ATGACAACACAAAACTTCCTCGCCGAGATCGGCACTGAAGAGCTGCCACCGAAGGCACTCAAAAAATTAGCGACTGCGTT
TGCAGAGAATATGGAATTGGAGCTAAACCAAGCGGGTTTGGCGTTTGAAAGCGTGCAATGGTTTGCCGCACCACGTCGTT
TGGCGGTGAAAGTGTTGGCGTTAGCCACCAGCCAGCCAAGCAAAGAGATTGAAAAACGTGGGCCGGCAGTGTCTGCGGCA
TTTGATGCGGAAGGTAAACCAACTAAAGCGGCTGAAGGTTGGGCACGTGGTTGTGGGATCAGCGTTGAGCAAGCTGAGCG
TGTTGCCACTGACAAAGGTGAGTGGTTAGTGCATCGTGCCACAATCGAAGGTCAACCGACCAAAAACTTATTGAAAGACA
TGGTGGCGAATGCGTTGGCGAAGTTGCCTATTCCAAAACCGATGCGTTGGGCGGACAAAACTGTGCAATTTATTCGTCCT
GTTCACACGGTGACGATGTTACTAGGCGATGAGCTGATTGAAGGCGAAATTTTAGGCGTGGAAAGCGCTCGTACCCTTCG
TGGTCACCGTTTCCTTGGTGAGCGTGAGTTCCAAATTTCACACGCGGATCAATACCCGGCGCTATTAAAAGAAAAAGGCT
CAGTAGTAGCTGACTTCAATGATCGTAAAGCGTTAATTTTGGCGAAATCGCAAGAAAAAGCGACCGCACTTGGTGGCGTA
GCAGATATTGAAGACGATTTACTTGATGAAGTCACCTCATTGGTGGAATATCCAAATGTATTAGCGGCGAAATTTGAAGA
ACGTTTCCTTGCTGTCCCAGCGGAAGCCTTGGTTTATACCATGAAAGGTGACCAAAAATATTTCCCAATTTATGACAAAG
ACGGCAAATTATTACCGCACTTTATTTTTGTGTCGAACATTAACCCTGACGATCCAAGCAAGATCATTGAAGGGAATGAA
AAAGTGGTGCGTCCACGTTTAACCGATGCGGAATTTTTCTTTAAAACCGACTTAAAACAACGTTTAGAAGATCAATTACC
ACGTTTAGAAACCGTGTTATTCCAACAACAGCTTGGTACATTACGTGATAAAACGGCACGTATTGAACAACTGGCTGGCG
AAATTGCAAAACAAATCGGTGCTGATGAAGTCAAAGCCAAACGCGCTGGTTTATTGTCAAAATGTGACTTAATGACCAAC
ATGGTGTTTGAATTTACTGACACGCAAGGTGTCATGGGGATGCATTATGCTCGTCACGATGGTGAAGATGAAGAAGTAGC
AGTAGCATTAAATGAACAATATATGCCACGTTTTGCTGGCGATGAGTTACCAAAATCCTTAGTCGCCAGTGCGGTAGCAT
TAGCCGATAAATTCGATACGCTCACCGGAATCTTCGGGATCGGTCAACAACCCAAAGGCAGTGCAGACCCGTTTGCCTTA
CGTCGTGCCGCATTAGGTGCATTACGCATTATTGTGGAGAAAAATTTACCGCTAGATTTAGCCGAAATTGTGAAAAAATC
GAGCGCCCTTTTTGGCGATAAATTAACAAATGCGAATGTCGTGGAAGATGTAGTTGAGTTTATGCTCGGTCGTTTCCGTG
CTTGGTATCAAGATGAAGGGATTGCCGTGGATGTGATTCAAGCGGTGCTTGCTCGTCGTCCAACCAAACCATCGGACTTT
GATGCCCGTGTACGTGCGGTGTCACACTTCCGTGCGTTAGAGGCGGCAGAGGCTTTAGCGGCAGCCAATAAGCGTGTAAG
CAATATTTTAGCGAAAGTTGAAGGGGAATTACCAGCCAATATTGATACCACATTATGTGCAGAAGCAGCAGAAAAAGTGT
TAGCTGAACAGGTGATTGCGTTACAAGCTGAGCTTGCGCCACTATTTGCTAAAGGCGAATACCAAGTGGCACTGGATCGT
TTAGCGGCATTGCGTGAGCCCGTGGATACTTTCTTCGATAATGTCATGGTGAACGCGGAAAATCCACAATTACGCCAAAA
CCGTTTAGCAATCTTAAATAACCTACGTAATCTATTCTTACAAGTGGCAGATATTTCCCTCTTACAATAA

Upstream 100 bases:

>100_bases
ATTATCATGCGGAATTTGGAACTGATGTTAAAACAGAAAAAATAACAATTAAATGTACGATAAAGAAATAGAATCTTGAT
ATTTTTTAAAGAGAAACAAA

Downstream 100 bases:

>100_bases
TAGATAACTGCATCACGAAATGGCACGGTAAAGTGCCATTTTTTCTTGTCGAGGTATAACAAAGAAAATTTCCAAAAAAG
AAACCGCACTTTAAGCAAGC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MTTQNFLAEIGTEELPPKALKKLATAFAENMELELNQAGLAFESVQWFAAPRRLAVKVLALATSQPSKEIEKRGPAVSAA
FDAEGKPTKAAEGWARGCGISVEQAERVATDKGEWLVHRATIEGQPTKNLLKDMVANALAKLPIPKPMRWADKTVQFIRP
VHTVTMLLGDELIEGEILGVESARTLRGHRFLGEREFQISHADQYPALLKEKGSVVADFNDRKALILAKSQEKATALGGV
ADIEDDLLDEVTSLVEYPNVLAAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPHFIFVSNINPDDPSKIIEGNE
KVVRPRLTDAEFFFKTDLKQRLEDQLPRLETVLFQQQLGTLRDKTARIEQLAGEIAKQIGADEVKAKRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQQPKGSADPFAL
RRAALGALRIIVEKNLPLDLAEIVKKSSALFGDKLTNANVVEDVVEFMLGRFRAWYQDEGIAVDVIQAVLARRPTKPSDF
DARVRAVSHFRALEAAEALAAANKRVSNILAKVEGELPANIDTTLCAEAAEKVLAEQVIALQAELAPLFAKGEYQVALDR
LAALREPVDTFFDNVMVNAENPQLRQNRLAILNNLRNLFLQVADISLLQ

Sequences:

>Translated_689_residues
MTTQNFLAEIGTEELPPKALKKLATAFAENMELELNQAGLAFESVQWFAAPRRLAVKVLALATSQPSKEIEKRGPAVSAA
FDAEGKPTKAAEGWARGCGISVEQAERVATDKGEWLVHRATIEGQPTKNLLKDMVANALAKLPIPKPMRWADKTVQFIRP
VHTVTMLLGDELIEGEILGVESARTLRGHRFLGEREFQISHADQYPALLKEKGSVVADFNDRKALILAKSQEKATALGGV
ADIEDDLLDEVTSLVEYPNVLAAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPHFIFVSNINPDDPSKIIEGNE
KVVRPRLTDAEFFFKTDLKQRLEDQLPRLETVLFQQQLGTLRDKTARIEQLAGEIAKQIGADEVKAKRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQQPKGSADPFAL
RRAALGALRIIVEKNLPLDLAEIVKKSSALFGDKLTNANVVEDVVEFMLGRFRAWYQDEGIAVDVIQAVLARRPTKPSDF
DARVRAVSHFRALEAAEALAAANKRVSNILAKVEGELPANIDTTLCAEAAEKVLAEQVIALQAELAPLFAKGEYQVALDR
LAALREPVDTFFDNVMVNAENPQLRQNRLAILNNLRNLFLQVADISLLQ
>Mature_688_residues
TTQNFLAEIGTEELPPKALKKLATAFAENMELELNQAGLAFESVQWFAAPRRLAVKVLALATSQPSKEIEKRGPAVSAAF
DAEGKPTKAAEGWARGCGISVEQAERVATDKGEWLVHRATIEGQPTKNLLKDMVANALAKLPIPKPMRWADKTVQFIRPV
HTVTMLLGDELIEGEILGVESARTLRGHRFLGEREFQISHADQYPALLKEKGSVVADFNDRKALILAKSQEKATALGGVA
DIEDDLLDEVTSLVEYPNVLAAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPHFIFVSNINPDDPSKIIEGNEK
VVRPRLTDAEFFFKTDLKQRLEDQLPRLETVLFQQQLGTLRDKTARIEQLAGEIAKQIGADEVKAKRAGLLSKCDLMTNM
VFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQQPKGSADPFALR
RAALGALRIIVEKNLPLDLAEIVKKSSALFGDKLTNANVVEDVVEFMLGRFRAWYQDEGIAVDVIQAVLARRPTKPSDFD
ARVRAVSHFRALEAAEALAAANKRVSNILAKVEGELPANIDTTLCAEAAEKVLAEQVIALQAELAPLFAKGEYQVALDRL
AALREPVDTFFDNVMVNAENPQLRQNRLAILNNLRNLFLQVADISLLQ

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=69.2307692307692, Blast_Score=966, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_PASMU (P57905)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246039.1
- ProteinModelPortal:   P57905
- GeneID:   1244449
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1102
- NMPDR:   fig|272843.1.peg.1102
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- BioCyc:   PMUL272843:PM1102-MONOMER
- BRENDA:   6.1.1.14
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 76020; Mature: 75889

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQNFLAEIGTEELPPKALKKLATAFAENMELELNQAGLAFESVQWFAAPRRLAVKVLA
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHH
LATSQPSKEIEKRGPAVSAAFDAEGKPTKAAEGWARGCGISVEQAERVATDKGEWLVHRA
HHCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEE
TIEGQPTKNLLKDMVANALAKLPIPKPMRWADKTVQFIRPVHTVTMLLGDELIEGEILGV
EECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
ESARTLRGHRFLGEREFQISHADQYPALLKEKGSVVADFNDRKALILAKSQEKATALGGV
CCHHHHHHHHCCCCCCEEECCCCCCHHHHHCCCCEEEECCCCEEEEEECCCHHHHHHCCC
ADIEDDLLDEVTSLVEYPNVLAAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHEEEEECCCCEECCEECCCCCCCCE
FIFVSNINPDDPSKIIEGNEKVVRPRLTDAEFFFKTDLKQRLEDQLPRLETVLFQQQLGT
EEEEECCCCCCCHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRDKTARIEQLAGEIAKQIGADEVKAKRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCC
GEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQQPKGSADPFAL
CCCCEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGALRIIVEKNLPLDLAEIVKKSSALFGDKLTNANVVEDVVEFMLGRFRAWYQDEG
HHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
IAVDVIQAVLARRPTKPSDFDARVRAVSHFRALEAAEALAAANKRVSNILAKVEGELPAN
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IDTTLCAEAAEKVLAEQVIALQAELAPLFAKGEYQVALDRLAALREPVDTFFDNVMVNAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEECCC
NPQLRQNRLAILNNLRNLFLQVADISLLQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTQNFLAEIGTEELPPKALKKLATAFAENMELELNQAGLAFESVQWFAAPRRLAVKVLA
CCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHH
LATSQPSKEIEKRGPAVSAAFDAEGKPTKAAEGWARGCGISVEQAERVATDKGEWLVHRA
HHCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEE
TIEGQPTKNLLKDMVANALAKLPIPKPMRWADKTVQFIRPVHTVTMLLGDELIEGEILGV
EECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
ESARTLRGHRFLGEREFQISHADQYPALLKEKGSVVADFNDRKALILAKSQEKATALGGV
CCHHHHHHHHCCCCCCEEECCCCCCHHHHHCCCCEEEECCCCEEEEEECCCHHHHHHCCC
ADIEDDLLDEVTSLVEYPNVLAAKFEERFLAVPAEALVYTMKGDQKYFPIYDKDGKLLPH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHEEEEECCCCEECCEECCCCCCCCE
FIFVSNINPDDPSKIIEGNEKVVRPRLTDAEFFFKTDLKQRLEDQLPRLETVLFQQQLGT
EEEEECCCCCCCHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRDKTARIEQLAGEIAKQIGADEVKAKRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCC
GEDEEVAVALNEQYMPRFAGDELPKSLVASAVALADKFDTLTGIFGIGQQPKGSADPFAL
CCCCEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGALRIIVEKNLPLDLAEIVKKSSALFGDKLTNANVVEDVVEFMLGRFRAWYQDEG
HHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
IAVDVIQAVLARRPTKPSDFDARVRAVSHFRALEAAEALAAANKRVSNILAKVEGELPAN
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IDTTLCAEAAEKVLAEQVIALQAELAPLFAKGEYQVALDRLAALREPVDTFFDNVMVNAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEECCC
NPQLRQNRLAILNNLRNLFLQVADISLLQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100