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The map label for this gene is dapE

Identifier: 15602887

GI number: 15602887

Start: 1204408

End: 1205544

Strand: Reverse

Name: dapE

Synonym: PM1022

Alternate gene names: 15602887

Gene position: 1205544-1204408 (Counterclockwise)

Preceding gene: 15602888

Following gene: 15602886

Centisome position: 53.4

GC content: 43.98

Gene sequence:

>1137_bases
ATGAAAAATTCCATTATTGAATTAGCCCGTGAATTGATTCGCCGACCATCCATTAGCCCTGACGATCAAGGTTGTCAACA
GATCATCGCTGAGCGTTTAGAACGCTTAGGTTTTCAAATCGAATGGCTCCCTTTTAATGATACGTTAAATTTATGGGCGA
AACATGGTTCGGGTTCGCCAGTCATTGCCTTTGCAGGACATACCGATGTGGTGCCTGTGGGGGATACAACCCAATGGCAG
TATCCGCCTTTTTCCGCACAGCTAGTGGATAATGTGCTGTATGGACGTGGTGCGGCAGACATGAAAGGATCGCTTGCGGC
GATGGTGGTGGCGGCAGAACACTATGTGAAAGCCAATCCAGAGCATTCAGGTACTGTTGCTTTATTAATTACCTCAGATG
AAGAAGCCGCCGCTAAAGATGGTACAGTACGTGTAGTCGAGACATTAATGGCAAGAGGTGAGCCGATTGATTATTGTATT
GTCGGCGAGCCTTCAAGTGCTCAGCAGTTTGGTGATATTGTCAAAAATGGTCGCCGTGGTTCGATTACAGCAAACCTTTA
TATTCAAGGGATACAAGGGCATGTGGCTTATCCGCATTTAGCACAAAATCCAGTGCATAAGGCACTTGGCTTTTTAACGG
AATTGACCACTTATCAATGGGATAACGGTAATGACTTTTTTCCACCGACTTCATTGCAAATTGCTAATATTCAGGCGGGA
ACAGGTAGCAATAATGTGATCCCGGGTGAGCTTTATGTGCAATTTAATTTACGCTATTGCACAGAAGTGACCGATGACAT
CATTAAAAAGAAAGTTGCAGAGATGTTGGCAAAACATCAGTTAAATTATCGTATTGAGTGGCATCTATCGGGCAAACCGT
TTTTAACAGCGAAAGGCAAATTGGTGGATACCCTGCTAGATGTCGTGGAAAAAATCACGCAAAACCGACCGCACTTAGAT
ACCGGTGGTGGCACGTCAGATGCCCGTTTTATTGCGTTAATGGGGGCAGAAGTGGTGGAATTTGGACCGTTGAATAAAAC
TATTCATAAAGTCGATGAATGTGTCAATGTAGATGATCTGGCAAAATGTGGTGAAGTATATCAGCACGTTTTGTGTAATA
TGTTGGAACGCGTATGA

Upstream 100 bases:

>100_bases
TATGCAGCCTGTTTTAAATAAGGCGATCGTGGCATGGTTGCCCTGATCTTTTTTGACAGAAACCCTGTTTTACCTTCTTG
AGCAAAAGTGCGGTATAAAA

Downstream 100 bases:

>100_bases
GCTTTCTCTTTACACCCGAGCAATTAACCGGTAAGGCACGCACACATTTAGTTGCACTCCCTTGCCCATTTTCTGTCCAT
CATTTCTTACACCAAGATTG

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKNSIIELARELIRRPSISPDDQGCQQIIAERLERLGFQIEWLPFNDTLNLWAKHGSGSPVIAFAGHTDVVPVGDTTQWQ
YPPFSAQLVDNVLYGRGAADMKGSLAAMVVAAEHYVKANPEHSGTVALLITSDEEAAAKDGTVRVVETLMARGEPIDYCI
VGEPSSAQQFGDIVKNGRRGSITANLYIQGIQGHVAYPHLAQNPVHKALGFLTELTTYQWDNGNDFFPPTSLQIANIQAG
TGSNNVIPGELYVQFNLRYCTEVTDDIIKKKVAEMLAKHQLNYRIEWHLSGKPFLTAKGKLVDTLLDVVEKITQNRPHLD
TGGGTSDARFIALMGAEVVEFGPLNKTIHKVDECVNVDDLAKCGEVYQHVLCNMLERV

Sequences:

>Translated_378_residues
MKNSIIELARELIRRPSISPDDQGCQQIIAERLERLGFQIEWLPFNDTLNLWAKHGSGSPVIAFAGHTDVVPVGDTTQWQ
YPPFSAQLVDNVLYGRGAADMKGSLAAMVVAAEHYVKANPEHSGTVALLITSDEEAAAKDGTVRVVETLMARGEPIDYCI
VGEPSSAQQFGDIVKNGRRGSITANLYIQGIQGHVAYPHLAQNPVHKALGFLTELTTYQWDNGNDFFPPTSLQIANIQAG
TGSNNVIPGELYVQFNLRYCTEVTDDIIKKKVAEMLAKHQLNYRIEWHLSGKPFLTAKGKLVDTLLDVVEKITQNRPHLD
TGGGTSDARFIALMGAEVVEFGPLNKTIHKVDECVNVDDLAKCGEVYQHVLCNMLERV
>Mature_378_residues
MKNSIIELARELIRRPSISPDDQGCQQIIAERLERLGFQIEWLPFNDTLNLWAKHGSGSPVIAFAGHTDVVPVGDTTQWQ
YPPFSAQLVDNVLYGRGAADMKGSLAAMVVAAEHYVKANPEHSGTVALLITSDEEAAAKDGTVRVVETLMARGEPIDYCI
VGEPSSAQQFGDIVKNGRRGSITANLYIQGIQGHVAYPHLAQNPVHKALGFLTELTTYQWDNGNDFFPPTSLQIANIQAG
TGSNNVIPGELYVQFNLRYCTEVTDDIIKKKVAEMLAKHQLNYRIEWHLSGKPFLTAKGKLVDTLLDVVEKITQNRPHLD
TGGGTSDARFIALMGAEVVEFGPLNKTIHKVDECVNVDDLAKCGEVYQHVLCNMLERV

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=371, Percent_Identity=62.2641509433962, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI1790395, Length=267, Percent_Identity=31.4606741573034, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789236, Length=256, Percent_Identity=25, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17551016, Length=241, Percent_Identity=26.1410788381743, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_PASMU (Q9CM22)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245959.1
- ProteinModelPortal:   Q9CM22
- SMR:   Q9CM22
- MEROPS:   M20.010
- GeneID:   1244369
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1022
- NMPDR:   fig|272843.1.peg.1022
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- ProtClustDB:   PRK13009
- BioCyc:   PMUL272843:PM1022-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41592; Mature: 41592

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 69-69 ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSIIELARELIRRPSISPDDQGCQQIIAERLERLGFQIEWLPFNDTLNLWAKHGSGSP
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEHEEECCCCCC
VIAFAGHTDVVPVGDTTQWQYPPFSAQLVDNVLYGRGAADMKGSLAAMVVAAEHYVKANP
EEEECCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
EHSGTVALLITSDEEAAAKDGTVRVVETLMARGEPIDYCIVGEPSSAQQFGDIVKNGRRG
CCCCEEEEEEECCCHHHCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCC
SITANLYIQGIQGHVAYPHLAQNPVHKALGFLTELTTYQWDNGNDFFPPTSLQIANIQAG
CEEEEEEEEECCCCEECCCHHCCHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEEECC
TGSNNVIPGELYVQFNLRYCTEVTDDIIKKKVAEMLAKHQLNYRIEWHLSGKPFLTAKGK
CCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEECCH
LVDTLLDVVEKITQNRPHLDTGGGTSDARFIALMGAEVVEFGPLNKTIHKVDECVNVDDL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCHHHHCCCCHHHHHHHHHHCCHHHH
AKCGEVYQHVLCNMLERV
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNSIIELARELIRRPSISPDDQGCQQIIAERLERLGFQIEWLPFNDTLNLWAKHGSGSP
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEHEEECCCCCC
VIAFAGHTDVVPVGDTTQWQYPPFSAQLVDNVLYGRGAADMKGSLAAMVVAAEHYVKANP
EEEECCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCC
EHSGTVALLITSDEEAAAKDGTVRVVETLMARGEPIDYCIVGEPSSAQQFGDIVKNGRRG
CCCCEEEEEEECCCHHHCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCC
SITANLYIQGIQGHVAYPHLAQNPVHKALGFLTELTTYQWDNGNDFFPPTSLQIANIQAG
CEEEEEEEEECCCCEECCCHHCCHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEEECC
TGSNNVIPGELYVQFNLRYCTEVTDDIIKKKVAEMLAKHQLNYRIEWHLSGKPFLTAKGK
CCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEECCH
LVDTLLDVVEKITQNRPHLDTGGGTSDARFIALMGAEVVEFGPLNKTIHKVDECVNVDDL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCHHHHCCCCHHHHHHHHHHCCHHHH
AKCGEVYQHVLCNMLERV
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100