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The map label for this gene is nudF [H]

Identifier: 15602885

GI number: 15602885

Start: 1203107

End: 1203715

Strand: Reverse

Name: nudF [H]

Synonym: PM1020

Alternate gene names: 15602885

Gene position: 1203715-1203107 (Counterclockwise)

Preceding gene: 15602886

Following gene: 15602884

Centisome position: 53.32

GC content: 43.68

Gene sequence:

>609_bases
ATGGAAATTCAACAGTTTCGTCAGCAAGATATTGACATTCTTAAAGAAGAAACCTTATACCAAGGTTTTTTTCAATTAAA
AAAGATTCAATTTAAACACAAGCTGTTTGCTGGCGGATACAGTGGGGTAGTCACTCGTGAATTGTTAGTCAAAGGGGCCG
CATCTGCGGTGATTGCTTACGATCCGATTAAAGATGCCGTTGTATTGGTTGAGCAAGTGCGTATTGGTGCTTATCAACCT
GATTCAGCACAGTCACCTTGGTTGTTAGAGTTGATCGCCGGCATGGTAGAAGAAGGAGAAAAACCAGAAGAGGTTGCGCT
ACGTGAAAGCGAAGAAGAAGCCGGCGTGCAAGTGCAAGATTTGCAACATTGTTTAAGTGTGTGGGACAGCCCAGGTGGTG
TGCTTGAGCGTATCCATTTGTTTGTGGGCAAAGTGGATAGCACAACAGCGAAAGGGCTACACGGTTTGAGCGAAGAGAAT
GAAGATATCCGTGTCCATGTAGTGAGCCGTGAGCAAGCTTATCAATGGGTTAATGAGGGCAAAATTGATAATAGCATTGC
GGTGTTGGGATTACAGTGGTTACAGTTGAATTATAAGACCCTCTCATAA

Upstream 100 bases:

>100_bases
ATTATGTGAGGCATGGCTGCAGGAAGAAATTGCGGGCAAAGCCTGTTTGCTTGCGAATCTCGCGCAGATTTTTTGTCGTT
TTTTTATTTAAGGAGATAAC

Downstream 100 bases:

>100_bases
TTCAATTTTCTAAATCCACACGATAAAAGTGTGATTTAGGTTTAATTTTCAGAAAATTTTTTATCAATAAATATGACATA
GCTAACATTTTTTTATTTTG

Product: hypothetical protein

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MEIQQFRQQDIDILKEETLYQGFFQLKKIQFKHKLFAGGYSGVVTRELLVKGAASAVIAYDPIKDAVVLVEQVRIGAYQP
DSAQSPWLLELIAGMVEEGEKPEEVALRESEEEAGVQVQDLQHCLSVWDSPGGVLERIHLFVGKVDSTTAKGLHGLSEEN
EDIRVHVVSREQAYQWVNEGKIDNSIAVLGLQWLQLNYKTLS

Sequences:

>Translated_202_residues
MEIQQFRQQDIDILKEETLYQGFFQLKKIQFKHKLFAGGYSGVVTRELLVKGAASAVIAYDPIKDAVVLVEQVRIGAYQP
DSAQSPWLLELIAGMVEEGEKPEEVALRESEEEAGVQVQDLQHCLSVWDSPGGVLERIHLFVGKVDSTTAKGLHGLSEEN
EDIRVHVVSREQAYQWVNEGKIDNSIAVLGLQWLQLNYKTLS
>Mature_202_residues
MEIQQFRQQDIDILKEETLYQGFFQLKKIQFKHKLFAGGYSGVVTRELLVKGAASAVIAYDPIKDAVVLVEQVRIGAYQP
DSAQSPWLLELIAGMVEEGEKPEEVALRESEEEAGVQVQDLQHCLSVWDSPGGVLERIHLFVGKVDSTTAKGLHGLSEEN
EDIRVHVVSREQAYQWVNEGKIDNSIAVLGLQWLQLNYKTLS

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=196, Percent_Identity=55.6122448979592, Blast_Score=233, Evalue=9e-63,
Organism=Escherichia coli, GI1788810, Length=200, Percent_Identity=28.5, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 22678; Mature: 22678

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIQQFRQQDIDILKEETLYQGFFQLKKIQFKHKLFAGGYSGVVTRELLVKGAASAVIAY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEE
DPIKDAVVLVEQVRIGAYQPDSAQSPWLLELIAGMVEEGEKPEEVALRESEEEAGVQVQD
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHCCCHHH
LQHCLSVWDSPGGVLERIHLFVGKVDSTTAKGLHGLSEENEDIRVHVVSREQAYQWVNEG
HHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHCC
KIDNSIAVLGLQWLQLNYKTLS
CCCCHHHHHHHHHHEEECEECC
>Mature Secondary Structure
MEIQQFRQQDIDILKEETLYQGFFQLKKIQFKHKLFAGGYSGVVTRELLVKGAASAVIAY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEE
DPIKDAVVLVEQVRIGAYQPDSAQSPWLLELIAGMVEEGEKPEEVALRESEEEAGVQVQD
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHCCCHHH
LQHCLSVWDSPGGVLERIHLFVGKVDSTTAKGLHGLSEENEDIRVHVVSREQAYQWVNEG
HHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHCC
KIDNSIAVLGLQWLQLNYKTLS
CCCCHHHHHHHHHHEEECEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10542272 [H]