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The map label for this gene is amsI [H]

Identifier: 15602882

GI number: 15602882

Start: 1199452

End: 1199895

Strand: Direct

Name: amsI [H]

Synonym: PM1017

Alternate gene names: 15602882

Gene position: 1199452-1199895 (Clockwise)

Preceding gene: 15602881

Following gene: 15602883

Centisome position: 53.13

GC content: 38.06

Gene sequence:

>444_bases
ATGTTTGAGCAAATTCTAGTCGTATGTATGGGAAATATCTGTCGTTCCCCTGTAGGGGAAAAATTACTGCAACACCATTT
CCCAGAAAAAAACATTACCTCTGCAGGTCTTGTCACTGAACGTAGTCGATTAGTTGACAAGCCTGCCGACGATATGATGA
TAAAAATTGCTAGCCAGCACGGATTAAATCTTTCGGCTCATCGTGCTAAGCAGTTGACACATGCATTATGTCATAAAGCC
GATTTGATTCTTGTTATGGAAAAAGCGCATATTGAATTAGTTCATCAAATATGCCCTTTATCTCAAGGTAAAGTCATGTT
GTTTGGACATGGATTAACTGCGTCAAAAGAGATTCCCGATCCCTATAAAAAGAGTCAAGAAATGTTTGAGTATGCTTATC
AAATGCTAGATAGCGCAGCATATCAATGGAAAAAAATGTTATAG

Upstream 100 bases:

>100_bases
CCAGTTGCCCGCTGGAATCGTGTGATTAGTCAGATCGTGCCTACTTTGTCAGGGTTCGACTCAATCACTGAAAGTATGTT
AAGAATCCGAAATTGGTAAT

Downstream 100 bases:

>100_bases
GATATGAAATAATGAACGAGAAAAACCATGAAGAAACACTTGATGTAATGAAATTCCTCGGCATTTTATTAGATCATAAA
GGGTGGATTGCCCTGTTTAC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MFEQILVVCMGNICRSPVGEKLLQHHFPEKNITSAGLVTERSRLVDKPADDMMIKIASQHGLNLSAHRAKQLTHALCHKA
DLILVMEKAHIELVHQICPLSQGKVMLFGHGLTASKEIPDPYKKSQEMFEYAYQMLDSAAYQWKKML

Sequences:

>Translated_147_residues
MFEQILVVCMGNICRSPVGEKLLQHHFPEKNITSAGLVTERSRLVDKPADDMMIKIASQHGLNLSAHRAKQLTHALCHKA
DLILVMEKAHIELVHQICPLSQGKVMLFGHGLTASKEIPDPYKKSQEMFEYAYQMLDSAAYQWKKML
>Mature_147_residues
MFEQILVVCMGNICRSPVGEKLLQHHFPEKNITSAGLVTERSRLVDKPADDMMIKIASQHGLNLSAHRAKQLTHALCHKA
DLILVMEKAHIELVHQICPLSQGKVMLFGHGLTASKEIPDPYKKSQEMFEYAYQMLDSAAYQWKKML

Specific function: May function as a phosphatase required for amylovoran (an exopolysaccharide that functions as a virulence factor) production [H]

COG id: COG0394

COG function: function code T; Protein-tyrosine-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the low molecular weight phosphotyrosine protein phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788375, Length=147, Percent_Identity=50.3401360544218, Blast_Score=147, Evalue=4e-37,
Organism=Escherichia coli, GI87081812, Length=146, Percent_Identity=47.945205479452, Blast_Score=130, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000106
- InterPro:   IPR017867 [H]

Pfam domain/function: PF01451 LMWPc [H]

EC number: =3.1.3.48 [H]

Molecular weight: Translated: 16671; Mature: 16671

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEQILVVCMGNICRSPVGEKLLQHHFPEKNITSAGLVTERSRLVDKPADDMMIKIASQH
CHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHC
GLNLSAHRAKQLTHALCHKADLILVMEKAHIELVHQICPLSQGKVMLFGHGLTASKEIPD
CCCCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
PYKKSQEMFEYAYQMLDSAAYQWKKML
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFEQILVVCMGNICRSPVGEKLLQHHFPEKNITSAGLVTERSRLVDKPADDMMIKIASQH
CHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHC
GLNLSAHRAKQLTHALCHKADLILVMEKAHIELVHQICPLSQGKVMLFGHGLTASKEIPD
CCCCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
PYKKSQEMFEYAYQMLDSAAYQWKKML
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596293 [H]