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The map label for this gene is miaA [H]

Identifier: 15602770

GI number: 15602770

Start: 1063595

End: 1064668

Strand: Direct

Name: miaA [H]

Synonym: PM0905

Alternate gene names: 15602770

Gene position: 1063595-1064668 (Clockwise)

Preceding gene: 15602769

Following gene: 15602771

Centisome position: 47.11

GC content: 43.58

Gene sequence:

>1074_bases
ATGCCGATGCCGTTACCTTACTCACTGAAACAGAGCAATTGCTTAATCAGAAACAAGCTATCCGATTATCCGAATTATTC
ATCGAGCTTAATTGGCAAGCTTACTTGGAAAAAATGTAATCACATGCCTTTAACAACACCTACTGCCATTTTCTTAATGG
GTCCCACTGCCTCTGGCAAAACAGACTTAGCAATTCAGTTGCGTCAGACGCTACCCGTTGAAGTTATCAGTGTCGATTCC
GCTTTAATCTATCGTGGTATGGATATTGGAACTGCCAAACCGAGCGCAGAAGAACTCGCTTTAGCGCCTCATCGTTTAAT
TGATATTTGTGATCCTGCTGAAAGTTACTCTGCGGCTAATTTTCGTCAAGATGCGCTACGGGAAATGGCCGATATCATTG
CAGCTGGCAAAATTCCGCTTTTAGTCGGCGGAACGATGTTGTATTACAAAGCCTTGTTAGAAGGGCTTTCCCCGTTGCCT
TCAGCAGATGAAAAAGTGCGGTCAGAAATTGAAGAAAAAGCGCAGCTGCAAGGCTGGGCAGCCCTACACCAAGAACTGGC
TAAGATCGATCCTCTCGCGGCACAACGCATTAATCCAAATGATTCGCAGCGTATTAATCGTGCGTTAGAAGTTTTCTATT
TAACCGGGAAATCCCTGAGCGAACTGAGCCAGCAAAAAGGGGACAGCCTCCCCTACCAAATCCTGCAATTTGCTATTGCA
CCGAAAGATCGCAGTATCTTACATGATCGCATTGCGTTACGTTTCCAAAAAATGATCGAACAGGGTTTCCAACAAGAAGT
TGAAAAGCTCTATCAACGTGAGGATTTACATTTGGATCTTCCTGCTATGCGCTGTGTAGGCTATCGACAAATGTGGGAAT
ATTTACGTGGTGATTACGATCACGACGAGATGATTTTCCGTGGTATTTGTGCCACTCGCCAGTTGGCAAAACGTCAAATT
ACTTGGTTACGAGGTTGGAAATATCCAATTGAATGGCTTGATAGTTTAGCAATCGAAAGTGCTAAACAAACGATTATTCA
TGCGGTAACGAAGATAAGTCATTCAAACAGTTAA

Upstream 100 bases:

>100_bases
CAGAAATGTATCATCGTGTTACTCTCGCAGCCTCTTGATACCTTTTCTCATTTCTTAACAACGCTTTGTGACGTGATCGA
ATTGGAACAAATAACGGTCT

Downstream 100 bases:

>100_bases
AAAATAATTACGGTTCAGTGATGACAATAAATTAATTTATTGTTATATTGAGATTTGGAGGAAATACAGAACTCTCTGTG
TTTAGCTAGTTGTATCATTT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MPMPLPYSLKQSNCLIRNKLSDYPNYSSSLIGKLTWKKCNHMPLTTPTAIFLMGPTASGKTDLAIQLRQTLPVEVISVDS
ALIYRGMDIGTAKPSAEELALAPHRLIDICDPAESYSAANFRQDALREMADIIAAGKIPLLVGGTMLYYKALLEGLSPLP
SADEKVRSEIEEKAQLQGWAALHQELAKIDPLAAQRINPNDSQRINRALEVFYLTGKSLSELSQQKGDSLPYQILQFAIA
PKDRSILHDRIALRFQKMIEQGFQQEVEKLYQREDLHLDLPAMRCVGYRQMWEYLRGDYDHDEMIFRGICATRQLAKRQI
TWLRGWKYPIEWLDSLAIESAKQTIIHAVTKISHSNS

Sequences:

>Translated_357_residues
MPMPLPYSLKQSNCLIRNKLSDYPNYSSSLIGKLTWKKCNHMPLTTPTAIFLMGPTASGKTDLAIQLRQTLPVEVISVDS
ALIYRGMDIGTAKPSAEELALAPHRLIDICDPAESYSAANFRQDALREMADIIAAGKIPLLVGGTMLYYKALLEGLSPLP
SADEKVRSEIEEKAQLQGWAALHQELAKIDPLAAQRINPNDSQRINRALEVFYLTGKSLSELSQQKGDSLPYQILQFAIA
PKDRSILHDRIALRFQKMIEQGFQQEVEKLYQREDLHLDLPAMRCVGYRQMWEYLRGDYDHDEMIFRGICATRQLAKRQI
TWLRGWKYPIEWLDSLAIESAKQTIIHAVTKISHSNS
>Mature_356_residues
PMPLPYSLKQSNCLIRNKLSDYPNYSSSLIGKLTWKKCNHMPLTTPTAIFLMGPTASGKTDLAIQLRQTLPVEVISVDSA
LIYRGMDIGTAKPSAEELALAPHRLIDICDPAESYSAANFRQDALREMADIIAAGKIPLLVGGTMLYYKALLEGLSPLPS
ADEKVRSEIEEKAQLQGWAALHQELAKIDPLAAQRINPNDSQRINRALEVFYLTGKSLSELSQQKGDSLPYQILQFAIAP
KDRSILHDRIALRFQKMIEQGFQQEVEKLYQREDLHLDLPAMRCVGYRQMWEYLRGDYDHDEMIFRGICATRQLAKRQIT
WLRGWKYPIEWLDSLAIESAKQTIIHAVTKISHSNS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=28.9389067524116, Blast_Score=125, Evalue=4e-29,
Organism=Escherichia coli, GI1790613, Length=309, Percent_Identity=66.0194174757282, Blast_Score=440, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI25144712, Length=303, Percent_Identity=29.042904290429, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324848, Length=299, Percent_Identity=29.4314381270903, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24649830, Length=277, Percent_Identity=32.4909747292419, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 40427; Mature: 40296

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMPLPYSLKQSNCLIRNKLSDYPNYSSSLIGKLTWKKCNHMPLTTPTAIFLMGPTASGK
CCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
TDLAIQLRQTLPVEVISVDSALIYRGMDIGTAKPSAEELALAPHRLIDICDPAESYSAAN
CCEEEEECCCCCEEEEECCHHHHHCCCCCCCCCCCHHHHHHCHHHHHHHCCCHHHHHHHH
FRQDALREMADIIAAGKIPLLVGGTMLYYKALLEGLSPLPSADEKVRSEIEEKAQLQGWA
HHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
ALHQELAKIDPLAAQRINPNDSQRINRALEVFYLTGKSLSELSQQKGDSLPYQILQFAIA
HHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHC
PKDRSILHDRIALRFQKMIEQGFQQEVEKLYQREDLHLDLPAMRCVGYRQMWEYLRGDYD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCC
HDEMIFRGICATRQLAKRQITWLRGWKYPIEWLDSLAIESAKQTIIHAVTKISHSNS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PMPLPYSLKQSNCLIRNKLSDYPNYSSSLIGKLTWKKCNHMPLTTPTAIFLMGPTASGK
CCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
TDLAIQLRQTLPVEVISVDSALIYRGMDIGTAKPSAEELALAPHRLIDICDPAESYSAAN
CCEEEEECCCCCEEEEECCHHHHHCCCCCCCCCCCHHHHHHCHHHHHHHCCCHHHHHHHH
FRQDALREMADIIAAGKIPLLVGGTMLYYKALLEGLSPLPSADEKVRSEIEEKAQLQGWA
HHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
ALHQELAKIDPLAAQRINPNDSQRINRALEVFYLTGKSLSELSQQKGDSLPYQILQFAIA
HHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHC
PKDRSILHDRIALRFQKMIEQGFQQEVEKLYQREDLHLDLPAMRCVGYRQMWEYLRGDYD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCC
HDEMIFRGICATRQLAKRQITWLRGWKYPIEWLDSLAIESAKQTIIHAVTKISHSNS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]