Click here to switch to the map view.

The map label for this gene is nagK

Identifier: 15602540

GI number: 15602540

Start: 783999

End: 784913

Strand: Reverse

Name: nagK

Synonym: PM0675

Alternate gene names: 15602540

Gene position: 784913-783999 (Counterclockwise)

Preceding gene: 15602541

Following gene: 15602539

Centisome position: 34.77

GC content: 40.22

Gene sequence:

>915_bases
ATGCGATATGGATTTGATATTGGCGGAACAAAAATTGAACTTGCCGTATTTAATGATCAGTTAGAAAAACAATATTCAGA
AAGAGTAGAAACCCCTAAAGAAAGTTATGAGGAATGGTTAAACACAATTGTGCGTTTAGTCCATAAAGCAGATGAGATGT
TTCAGTGTCAAGGCACTGTAGGATTAGGTATTCCCGGTTTTGTTAATCCAGCCACAGGTATTGCGGAAATAGTGAATATT
CGTGCGGCAGATCATAAACCGATTATTCAAGATCTTGAAACGCGATTAGGGCGTGAAGTTCGCGCTGAAAATGATGCGAA
TTGTTTTGCGTTATCAGAAGCTTGGGATGAGGAAAATGCCCAATATCCAAGCGTGCTAGGGCTAATTCTCGGGACTGGAT
TTGGGGGCGGTCTGATTTTTAATGGTAAGGTACATTCTGGTCAAACAGGGATGGCGGGTGAAGTAGGACATACACAATTA
AATTACCATGCGTTAAGATTGTTGGGCGGGGATAATGCACCGATTTATGATTGTGGTTGTGGTAATCGTGCTTGTTTAGA
TACGTATTTATCAGGCAGAGGCTTTGAAATGTTGTATCGTGATATGCAAGGTGAAGCATTAAGTGCGCAAGCGATTATTC
AACGTTTTTATGCCGGTGACCAAAGTGCGGTCAAATTTGTTGAACTTTTTATTGAGCTTTGTGCCATTTCAATAGGAAAT
TTGATTACAGTATTAGATCCTCACGTGATTGTTTTAGGTGGTGGATTATCTAATTTTGATTATTTATATGAAGCCTTACC
TAAAGCCCTACCTGCACGTTTATTACGCAGTGCGAATGTGCCACTAATTAAAAAAGCGAAGTATGGTGATTCTGGTGGTG
TACGAGGGGCCGCAGCGTTGTTTTTGAGCAAATAA

Upstream 100 bases:

>100_bases
TGGGGAATTTTTACGTTTTTGCAAAAAAAATCGATATTTATCAAGAAATAGACGTGAAATCATTGCTAGAATTAAAATTA
TTTTTTTAAGTGAGGTGAAC

Downstream 100 bases:

>100_bases
ATATGGCGAGATAGCGAGGTGGAAACATCTCGTTATTTTTTTATGTGATGCAACGGAATTGTTTATTTTTCTACGATACG
TTGAAATTTTATTCAAAAAT

Product: N-acetyl-D-glucosamine kinase

Products: NA

Alternate protein names: GlcNAc kinase

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MRYGFDIGGTKIELAVFNDQLEKQYSERVETPKESYEEWLNTIVRLVHKADEMFQCQGTVGLGIPGFVNPATGIAEIVNI
RAADHKPIIQDLETRLGREVRAENDANCFALSEAWDEENAQYPSVLGLILGTGFGGGLIFNGKVHSGQTGMAGEVGHTQL
NYHALRLLGGDNAPIYDCGCGNRACLDTYLSGRGFEMLYRDMQGEALSAQAIIQRFYAGDQSAVKFVELFIELCAISIGN
LITVLDPHVIVLGGGLSNFDYLYEALPKALPARLLRSANVPLIKKAKYGDSGGVRGAAALFLSK

Sequences:

>Translated_304_residues
MRYGFDIGGTKIELAVFNDQLEKQYSERVETPKESYEEWLNTIVRLVHKADEMFQCQGTVGLGIPGFVNPATGIAEIVNI
RAADHKPIIQDLETRLGREVRAENDANCFALSEAWDEENAQYPSVLGLILGTGFGGGLIFNGKVHSGQTGMAGEVGHTQL
NYHALRLLGGDNAPIYDCGCGNRACLDTYLSGRGFEMLYRDMQGEALSAQAIIQRFYAGDQSAVKFVELFIELCAISIGN
LITVLDPHVIVLGGGLSNFDYLYEALPKALPARLLRSANVPLIKKAKYGDSGGVRGAAALFLSK
>Mature_304_residues
MRYGFDIGGTKIELAVFNDQLEKQYSERVETPKESYEEWLNTIVRLVHKADEMFQCQGTVGLGIPGFVNPATGIAEIVNI
RAADHKPIIQDLETRLGREVRAENDANCFALSEAWDEENAQYPSVLGLILGTGFGGGLIFNGKVHSGQTGMAGEVGHTQL
NYHALRLLGGDNAPIYDCGCGNRACLDTYLSGRGFEMLYRDMQGEALSAQAIIQRFYAGDQSAVKFVELFIELCAISIGN
LITVLDPHVIVLGGGLSNFDYLYEALPKALPARLLRSANVPLIKKAKYGDSGGVRGAAALFLSK

Specific function: Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family. NagK subfamily

Homologues:

Organism=Escherichia coli, GI1787363, Length=303, Percent_Identity=49.8349834983498, Blast_Score=303, Evalue=7e-84,
Organism=Escherichia coli, GI87081733, Length=304, Percent_Identity=40.7894736842105, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1790522, Length=307, Percent_Identity=27.6872964169381, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786891, Length=275, Percent_Identity=21.4545454545455, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NAGK_PASMU (Q9CMX5)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245612.1
- HSSP:   Q8ZPZ9
- ProteinModelPortal:   Q9CMX5
- SMR:   Q9CMX5
- GeneID:   1244022
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0675
- NMPDR:   fig|272843.1.peg.675
- HOGENOM:   HBG730260
- OMA:   GMEDADD
- ProtClustDB:   PRK13310
- BioCyc:   PMUL272843:PM0675-MONOMER
- BRENDA:   2.7.1.59
- HAMAP:   MF_01271
- InterPro:   IPR000600

Pfam domain/function: PF00480 ROK

EC number: =2.7.1.59

Molecular weight: Translated: 33039; Mature: 33039

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYGFDIGGTKIELAVFNDQLEKQYSERVETPKESYEEWLNTIVRLVHKADEMFQCQGTV
CCCCCCCCCCEEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCC
GLGIPGFVNPATGIAEIVNIRAADHKPIIQDLETRLGREVRAENDANCFALSEAWDEENA
CCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHCCCCCCCEEEEHHHHCCCCC
QYPSVLGLILGTGFGGGLIFNGKVHSGQTGMAGEVGHTQLNYHALRLLGGDNAPIYDCGC
CCHHHHHHHHHCCCCCCEEECCEECCCCCCCCCCCCCCCHHHEEEEEECCCCCCEEECCC
GNRACLDTYLSGRGFEMLYRDMQGEALSAQAIIQRFYAGDQSAVKFVELFIELCAISIGN
CCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
LITVLDPHVIVLGGGLSNFDYLYEALPKALPARLLRSANVPLIKKAKYGDSGGVRGAAAL
EEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHH
FLSK
EECC
>Mature Secondary Structure
MRYGFDIGGTKIELAVFNDQLEKQYSERVETPKESYEEWLNTIVRLVHKADEMFQCQGTV
CCCCCCCCCCEEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCC
GLGIPGFVNPATGIAEIVNIRAADHKPIIQDLETRLGREVRAENDANCFALSEAWDEENA
CCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCHHHCCCCCCCEEEEHHHHCCCCC
QYPSVLGLILGTGFGGGLIFNGKVHSGQTGMAGEVGHTQLNYHALRLLGGDNAPIYDCGC
CCHHHHHHHHHCCCCCCEEECCEECCCCCCCCCCCCCCCHHHEEEEEECCCCCCEEECCC
GNRACLDTYLSGRGFEMLYRDMQGEALSAQAIIQRFYAGDQSAVKFVELFIELCAISIGN
CCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
LITVLDPHVIVLGGGLSNFDYLYEALPKALPARLLRSANVPLIKKAKYGDSGGVRGAAAL
EEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHH
FLSK
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100