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The map label for this gene is cysB [H]

Identifier: 15602525

GI number: 15602525

Start: 767591

End: 768562

Strand: Reverse

Name: cysB [H]

Synonym: PM0660

Alternate gene names: 15602525

Gene position: 768562-767591 (Counterclockwise)

Preceding gene: 15602526

Following gene: 15602523

Centisome position: 34.05

GC content: 38.48

Gene sequence:

>972_bases
ATGAAAATACAACAATTACGTTATATTGTAGAAATTGTTAATCAAAATTTAAATGTAACAGAGGCAGCAAACGCGCTTTT
TACTTCTCAACCGGGGATCAGCAAACAAGTCCGTTTATTAGAAGATGAACTCGGTTTAGAAATTTTTGAACGCAATGGGA
AGCATATTAAATCCTTAACGCCGGCAGGACAAAAAATCGTAGCGATTGCACGGGAGCTCTTAGTCAAAACACAAAGTATT
AGAGCGGTAGCAAATGAATACACTCAACCCAACCATGGCGTTTTGCGTATTGCTACCACTAATACACAAGCACGATATGT
GTTACCGCCAGTGATTGAACGTTTTTCAAAGAAGTATCCTGATGTCAGTTTACATATTCATCAAGGTTCACCAACGCAGA
TTTATGATGCGTTGTTATCTGGTGAAGTGGATATTGCGATTACCACTGAAGCGCAGTATTTATTTGATGATGTGGTGCTT
TTACCTTGTTATATGTGGAATCGTTCGGTACTGATTAAGCATGATCATCCCCTTGCACAATGTAAAAACCTGACCATTGA
AGAATTAGGAAAATATCCTCTTGTGACCTATACCTTTGGTTTTACGGGAGTGTCCGATTTGGATTATGCTTTTAATCGTG
CTGGGATTTTACCTAATATCGTTTTTACGGCTACCGATGCGGATGTGATCAAAACCTATGTCCGTTTAGGTTTAGGGGTG
GGCATTATGGCGTCTATGGCACATACAAAAGATGATGGTGATTTACTTGCTTTAAATGCGGATCATCTCTTTAATCCAAG
TACGGTACAAATTGCATTTAAACACAGCACTTTCTTACGTGCGTACATGTATGATTTTATTCAGTATTTCTCACCGCACT
TAACACGTGAATTAGTTGAAAAAGCAGAGCGATTACGTGATAACAATGCAGTGAAAAAACTGTTTGAAGGCATTGAATTA
GCGATACGTTAG

Upstream 100 bases:

>100_bases
GGTCAAATTCGTAAGGCTGTGAAGCGTTATGCTGAATTAAATAAACGTTATAAAAAATAAGTTTTTTCTTATACAGCCAG
TGCTGTGAGAGGATAACTGA

Downstream 100 bases:

>100_bases
TGCTGATGCGTTTTATTTTCAGTGCTGAAAACAGATAAAAAAATGCCCACCAAATTGGTGGGCATTTTTGTTAGTTGGGA
TTATTTGCGGACTTTAATTT

Product: transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKIQQLRYIVEIVNQNLNVTEAANALFTSQPGISKQVRLLEDELGLEIFERNGKHIKSLTPAGQKIVAIARELLVKTQSI
RAVANEYTQPNHGVLRIATTNTQARYVLPPVIERFSKKYPDVSLHIHQGSPTQIYDALLSGEVDIAITTEAQYLFDDVVL
LPCYMWNRSVLIKHDHPLAQCKNLTIEELGKYPLVTYTFGFTGVSDLDYAFNRAGILPNIVFTATDADVIKTYVRLGLGV
GIMASMAHTKDDGDLLALNADHLFNPSTVQIAFKHSTFLRAYMYDFIQYFSPHLTRELVEKAERLRDNNAVKKLFEGIEL
AIR

Sequences:

>Translated_323_residues
MKIQQLRYIVEIVNQNLNVTEAANALFTSQPGISKQVRLLEDELGLEIFERNGKHIKSLTPAGQKIVAIARELLVKTQSI
RAVANEYTQPNHGVLRIATTNTQARYVLPPVIERFSKKYPDVSLHIHQGSPTQIYDALLSGEVDIAITTEAQYLFDDVVL
LPCYMWNRSVLIKHDHPLAQCKNLTIEELGKYPLVTYTFGFTGVSDLDYAFNRAGILPNIVFTATDADVIKTYVRLGLGV
GIMASMAHTKDDGDLLALNADHLFNPSTVQIAFKHSTFLRAYMYDFIQYFSPHLTRELVEKAERLRDNNAVKKLFEGIEL
AIR
>Mature_323_residues
MKIQQLRYIVEIVNQNLNVTEAANALFTSQPGISKQVRLLEDELGLEIFERNGKHIKSLTPAGQKIVAIARELLVKTQSI
RAVANEYTQPNHGVLRIATTNTQARYVLPPVIERFSKKYPDVSLHIHQGSPTQIYDALLSGEVDIAITTEAQYLFDDVVL
LPCYMWNRSVLIKHDHPLAQCKNLTIEELGKYPLVTYTFGFTGVSDLDYAFNRAGILPNIVFTATDADVIKTYVRLGLGV
GIMASMAHTKDDGDLLALNADHLFNPSTVQIAFKHSTFLRAYMYDFIQYFSPHLTRELVEKAERLRDNNAVKKLFEGIEL
AIR

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=321, Percent_Identity=62.9283489096573, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1788296, Length=309, Percent_Identity=42.7184466019417, Blast_Score=257, Evalue=6e-70,
Organism=Escherichia coli, GI157672245, Length=242, Percent_Identity=28.9256198347107, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1790399, Length=265, Percent_Identity=27.5471698113208, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787879, Length=252, Percent_Identity=28.968253968254, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788887, Length=268, Percent_Identity=28.7313432835821, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI145693105, Length=188, Percent_Identity=25, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787601, Length=160, Percent_Identity=31.25, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1790262, Length=203, Percent_Identity=25.615763546798, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788481, Length=246, Percent_Identity=25.609756097561, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI2367136, Length=242, Percent_Identity=23.5537190082645, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788748, Length=261, Percent_Identity=23.3716475095785, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=23.5772357723577, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87082132, Length=126, Percent_Identity=29.3650793650794, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081904, Length=201, Percent_Identity=25.8706467661692, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36422; Mature: 36422

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQQLRYIVEIVNQNLNVTEAANALFTSQPGISKQVRLLEDELGLEIFERNGKHIKSLT
CCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHCCCCEEECCC
PAGQKIVAIARELLVKTQSIRAVANEYTQPNHGVLRIATTNTQARYVLPPVIERFSKKYP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCHHHHHHHHHCC
DVSLHIHQGSPTQIYDALLSGEVDIAITTEAQYLFDDVVLLPCYMWNRSVLIKHDHPLAQ
CEEEEEECCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCHHH
CKNLTIEELGKYPLVTYTFGFTGVSDLDYAFNRAGILPNIVFTATDADVIKTYVRLGLGV
HCCCCHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCH
GIMASMAHTKDDGDLLALNADHLFNPSTVQIAFKHSTFLRAYMYDFIQYFSPHLTRELVE
HHHHHHHCCCCCCCEEEEEHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHCHHHHHHHHH
KAERLRDNNAVKKLFEGIELAIR
HHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIQQLRYIVEIVNQNLNVTEAANALFTSQPGISKQVRLLEDELGLEIFERNGKHIKSLT
CCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHCCCCEEECCC
PAGQKIVAIARELLVKTQSIRAVANEYTQPNHGVLRIATTNTQARYVLPPVIERFSKKYP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCHHHHHHHHHCC
DVSLHIHQGSPTQIYDALLSGEVDIAITTEAQYLFDDVVLLPCYMWNRSVLIKHDHPLAQ
CEEEEEECCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCHHH
CKNLTIEELGKYPLVTYTFGFTGVSDLDYAFNRAGILPNIVFTATDADVIKTYVRLGLGV
HCCCCHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCH
GIMASMAHTKDDGDLLALNADHLFNPSTVQIAFKHSTFLRAYMYDFIQYFSPHLTRELVE
HHHHHHHCCCCCCCEEEEEHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHCHHHHHHHHH
KAERLRDNNAVKKLFEGIELAIR
HHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]