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The map label for this gene is truA

Identifier: 15602502

GI number: 15602502

Start: 739029

End: 739823

Strand: Reverse

Name: truA

Synonym: PM0637

Alternate gene names: 15602502

Gene position: 739823-739029 (Counterclockwise)

Preceding gene: 15602504

Following gene: 15602501

Centisome position: 32.77

GC content: 42.64

Gene sequence:

>795_bases
ATGAAAATTGCATTAGGCATTCAATATAACGGAAAAAATTATTGTGGTTGGCAACGTCAGGAAAATGTGGCGAGTGTACA
AGAGCAATTAGAAAAAGCGATTTCTTTTGTCGCTAACCAACCCTGTCAGATTTTTTGTGCAGGGCGAACGGATTCAGGGG
TGCATGCCACAGGGCAAGTTGTGCATTTTGAAACCGATGCCATTCGGGCAGAAAAAGCATGGAGTTTTGGTGTGAATGCG
AATTTGCCTGATGATATTTCAGTGAGCTGGGCAAAATGTGTTACTGATGATTTTCATGCCCGCTTTAGTGCTACTGCACG
CCGTTATCGCTATATTTTGTATTGTAATAAATTGCGCTCGGCAATTTTGCCTGAAGGTGTCACACATTGTCATTTAGACT
TAGATCACCACTTGATGCATCAAGCGGGACAAGCGTTATTAGGTGAACAAGACTTTTCTTCTTTTCGTGCAGCACAATGT
CAATCACATACACCATTTCGCAATGTTCATCATTTAAAAGTGGTGCGACGTGGGCAATATATTATTGTGGATATCCAAGC
CAATGCATTTGTTCATCACATGGTGCGTAATATTGTGGGCAGTCTGATTGAAGTCGGCGCGGGTCATCAACCTGTAGAAT
GGATAGGGTGGTTGCTGGCGCAAAAAGATCGTAGATTAGCGGCACCCACCGCAAAACCGGAAGGGCTTTATTTGGTCAAT
GTGAGTTATCCTACGCAATTTCAGTTACCTCAAAATGCGTTAGGGCCTTTGTTTTTAGAAAATGAATTAGGCTAG

Upstream 100 bases:

>100_bases
CCTGACTCAAATTGTGCCTGATTTTTCTGCTTATCGCTAGCGTAAATCTCCTCTATAATAGGGTGTTTTACAGCATCCTA
TTTAAAAAGATATATAAACC

Downstream 100 bases:

>100_bases
TCACATCAGACCTTTATTGCTAGCATATTGCCTTAAATTATGGTGTAATACATCGAATTTTCTCGTTAATAAAAAGGGTT
AACATGAGTTGGATTGATCG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKIALGIQYNGKNYCGWQRQENVASVQEQLEKAISFVANQPCQIFCAGRTDSGVHATGQVVHFETDAIRAEKAWSFGVNA
NLPDDISVSWAKCVTDDFHARFSATARRYRYILYCNKLRSAILPEGVTHCHLDLDHHLMHQAGQALLGEQDFSSFRAAQC
QSHTPFRNVHHLKVVRRGQYIIVDIQANAFVHHMVRNIVGSLIEVGAGHQPVEWIGWLLAQKDRRLAAPTAKPEGLYLVN
VSYPTQFQLPQNALGPLFLENELG

Sequences:

>Translated_264_residues
MKIALGIQYNGKNYCGWQRQENVASVQEQLEKAISFVANQPCQIFCAGRTDSGVHATGQVVHFETDAIRAEKAWSFGVNA
NLPDDISVSWAKCVTDDFHARFSATARRYRYILYCNKLRSAILPEGVTHCHLDLDHHLMHQAGQALLGEQDFSSFRAAQC
QSHTPFRNVHHLKVVRRGQYIIVDIQANAFVHHMVRNIVGSLIEVGAGHQPVEWIGWLLAQKDRRLAAPTAKPEGLYLVN
VSYPTQFQLPQNALGPLFLENELG
>Mature_264_residues
MKIALGIQYNGKNYCGWQRQENVASVQEQLEKAISFVANQPCQIFCAGRTDSGVHATGQVVHFETDAIRAEKAWSFGVNA
NLPDDISVSWAKCVTDDFHARFSATARRYRYILYCNKLRSAILPEGVTHCHLDLDHHLMHQAGQALLGEQDFSSFRAAQC
QSHTPFRNVHHLKVVRRGQYIIVDIQANAFVHHMVRNIVGSLIEVGAGHQPVEWIGWLLAQKDRRLAAPTAKPEGLYLVN
VSYPTQFQLPQNALGPLFLENELG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=270, Percent_Identity=26.2962962962963, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1788657, Length=260, Percent_Identity=67.3076923076923, Blast_Score=370, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24648455, Length=269, Percent_Identity=26.3940520446097, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281362146, Length=269, Percent_Identity=26.3940520446097, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_PASMU (P57861)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245574.1
- ProteinModelPortal:   P57861
- SMR:   P57861
- GeneID:   1243984
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0637
- NMPDR:   fig|272843.1.peg.637
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   PMUL272843:PM0637-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29642; Mature: 29642

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIALGIQYNGKNYCGWQRQENVASVQEQLEKAISFVANQPCQIFCAGRTDSGVHATGQV
CEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEE
VHFETDAIRAEKAWSFGVNANLPDDISVSWAKCVTDDFHARFSATARRYRYILYCNKLRS
EEEECHHHHHHHCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEHHHHH
AILPEGVTHCHLDLDHHLMHQAGQALLGEQDFSSFRAAQCQSHTPFRNVHHLKVVRRGQY
HHCCCCCEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHEEEECCCE
IIVDIQANAFVHHMVRNIVGSLIEVGAGHQPVEWIGWLLAQKDRRLAAPTAKPEGLYLVN
EEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEECCCCCCCCEEEEE
VSYPTQFQLPQNALGPLFLENELG
ECCCCEECCCCCCCCCEEECCCCC
>Mature Secondary Structure
MKIALGIQYNGKNYCGWQRQENVASVQEQLEKAISFVANQPCQIFCAGRTDSGVHATGQV
CEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEE
VHFETDAIRAEKAWSFGVNANLPDDISVSWAKCVTDDFHARFSATARRYRYILYCNKLRS
EEEECHHHHHHHCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEHHHHH
AILPEGVTHCHLDLDHHLMHQAGQALLGEQDFSSFRAAQCQSHTPFRNVHHLKVVRRGQY
HHCCCCCEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHEEEECCCE
IIVDIQANAFVHHMVRNIVGSLIEVGAGHQPVEWIGWLLAQKDRRLAAPTAKPEGLYLVN
EEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEECCCCCCCCEEEEE
VSYPTQFQLPQNALGPLFLENELG
ECCCCEECCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100