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The map label for this gene is cspD [H]

Identifier: 15602346

GI number: 15602346

Start: 564068

End: 564277

Strand: Direct

Name: cspD [H]

Synonym: PM0481

Alternate gene names: 15602346

Gene position: 564068-564277 (Clockwise)

Preceding gene: 15602345

Following gene: 15602348

Centisome position: 24.99

GC content: 39.05

Gene sequence:

>210_bases
ATGGAAGTTGGTGTAGTTAAATGGTTCAATAACGCGAAAGGATTTGGATTCATTTCTGCAGAAGGAACAGACGCAGATAT
TTTTGCGCATTACTCAGTCATCGAAATGGAAGGCTACCGTTCATTAAAAGCGGGTCAAAAAGTGCAATTTGAAGTCGTAC
ATGGCGATAAAGGATCACATGCGACGAAAATTATTCCAATCGTTGAATAA

Upstream 100 bases:

>100_bases
TTCCTAGAAGATTACCCTGAATAAAATTTGGGTGGTAAGACAATCTTGTCTTATTAAAATAAAAATAGTTAAAGTCGTTA
GACGATTAGAGGTTTTAATT

Downstream 100 bases:

>100_bases
GGTTTTCTCTTAACATTTAGTCATCGTAACAAAAAAGACAAGGATGTAACCTTGTCTTTTTTTCTCGGTTCATTTTAATA
AGTCTTTGAGCCCTGCTTTC

Product: CspD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 69; Mature: 69

Protein sequence:

>69_residues
MEVGVVKWFNNAKGFGFISAEGTDADIFAHYSVIEMEGYRSLKAGQKVQFEVVHGDKGSHATKIIPIVE

Sequences:

>Translated_69_residues
MEVGVVKWFNNAKGFGFISAEGTDADIFAHYSVIEMEGYRSLKAGQKVQFEVVHGDKGSHATKIIPIVE
>Mature_69_residues
MEVGVVKWFNNAKGFGFISAEGTDADIFAHYSVIEMEGYRSLKAGQKVQFEVVHGDKGSHATKIIPIVE

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=68.6567164179104, Blast_Score=101, Evalue=9e-24,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=46.875, Blast_Score=73, Evalue=4e-15,
Organism=Escherichia coli, GI1789979, Length=65, Percent_Identity=46.1538461538462, Blast_Score=68, Evalue=1e-13,
Organism=Escherichia coli, GI1787839, Length=63, Percent_Identity=49.2063492063492, Blast_Score=66, Evalue=5e-13,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=45.3125, Blast_Score=65, Evalue=6e-13,
Organism=Escherichia coli, GI2367114, Length=65, Percent_Identity=38.4615384615385, Blast_Score=64, Evalue=2e-12,
Organism=Escherichia coli, GI1787834, Length=65, Percent_Identity=38.4615384615385, Blast_Score=60, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7586; Mature: 7586

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVGVVKWFNNAKGFGFISAEGTDADIFAHYSVIEMEGYRSLKAGQKVQFEVVHGDKGSH
CCCEEEEECCCCCCCEEEEECCCCCEEEEEEEEEEECCCCCCCCCCEEEEEEEECCCCCC
ATKIIPIVE
CEEEEEEEC
>Mature Secondary Structure
MEVGVVKWFNNAKGFGFISAEGTDADIFAHYSVIEMEGYRSLKAGQKVQFEVVHGDKGSH
CCCEEEEECCCCCCCEEEEECCCCCEEEEEEEEEEECCCCCCCCCCEEEEEEEECCCCCC
ATKIIPIVE
CEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]